Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0569.Seq
(786 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 150 9e-37
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 150 9e-37
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 150 9e-37
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 150 9e-37
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 99 2e-21
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 55 5e-08
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 48 5e-06
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 37 0.013
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 33 0.21
At4g27900.2 68417.m04005 expressed protein 32 0.50
At4g27900.1 68417.m04004 expressed protein 32 0.50
At1g46840.1 68414.m05218 F-box family protein contains F-box dom... 32 0.50
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 4.6
At4g03430.1 68417.m00470 pre-mRNA splicing factor-related simila... 28 6.1
At5g42860.1 68418.m05224 expressed protein 25 7.2
At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 28 8.1
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 150 bits (364), Expect = 9e-37
Identities = 65/84 (77%), Positives = 78/84 (92%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 581
VKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++N
Sbjct: 277 VKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMN 336
Query: 580 HPGQISNGYTPVLDCHTAHIACKF 509
HPGQI NGY PVLDCHT+HIA KF
Sbjct: 337 HPGQIGNGYAPVLDCHTSHIAVKF 360
Score = 119 bits (287), Expect = 2e-27
Identities = 57/94 (60%), Positives = 72/94 (76%), Gaps = 1/94 (1%)
Frame = -2
Query: 533 HCPH-CLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 357
H H ++ SEI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGR
Sbjct: 352 HTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGR 411
Query: 356 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEK 255
FAVRDMRQTVAVGVIK+V+ K+ G KVTKAA K
Sbjct: 412 FAVRDMRQTVAVGVIKSVDKKDPTGAKVTKAAVK 445
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 150 bits (364), Expect = 9e-37
Identities = 65/84 (77%), Positives = 78/84 (92%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 581
VKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++N
Sbjct: 277 VKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMN 336
Query: 580 HPGQISNGYTPVLDCHTAHIACKF 509
HPGQI NGY PVLDCHT+HIA KF
Sbjct: 337 HPGQIGNGYAPVLDCHTSHIAVKF 360
Score = 119 bits (287), Expect = 2e-27
Identities = 57/94 (60%), Positives = 72/94 (76%), Gaps = 1/94 (1%)
Frame = -2
Query: 533 HCPH-CLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 357
H H ++ SEI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGR
Sbjct: 352 HTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGR 411
Query: 356 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEK 255
FAVRDMRQTVAVGVIK+V+ K+ G KVTKAA K
Sbjct: 412 FAVRDMRQTVAVGVIKSVDKKDPTGAKVTKAAVK 445
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 150 bits (364), Expect = 9e-37
Identities = 65/84 (77%), Positives = 78/84 (92%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 581
VKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++N
Sbjct: 277 VKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMN 336
Query: 580 HPGQISNGYTPVLDCHTAHIACKF 509
HPGQI NGY PVLDCHT+HIA KF
Sbjct: 337 HPGQIGNGYAPVLDCHTSHIAVKF 360
Score = 119 bits (287), Expect = 2e-27
Identities = 57/94 (60%), Positives = 72/94 (76%), Gaps = 1/94 (1%)
Frame = -2
Query: 533 HCPH-CLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 357
H H ++ SEI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGR
Sbjct: 352 HTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGR 411
Query: 356 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEK 255
FAVRDMRQTVAVGVIK+V+ K+ G KVTKAA K
Sbjct: 412 FAVRDMRQTVAVGVIKSVDKKDPTGAKVTKAAVK 445
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 150 bits (364), Expect = 9e-37
Identities = 65/84 (77%), Positives = 78/84 (92%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 581
VKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++N
Sbjct: 277 VKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMN 336
Query: 580 HPGQISNGYTPVLDCHTAHIACKF 509
HPGQI NGY PVLDCHT+HIA KF
Sbjct: 337 HPGQIGNGYAPVLDCHTSHIAVKF 360
Score = 119 bits (287), Expect = 2e-27
Identities = 57/94 (60%), Positives = 72/94 (76%), Gaps = 1/94 (1%)
Frame = -2
Query: 533 HCPH-CLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 357
H H ++ SEI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGR
Sbjct: 352 HTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGR 411
Query: 356 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEK 255
FAVRDMRQTVAVGVIK+V+ K+ G KVTKAA K
Sbjct: 412 FAVRDMRQTVAVGVIKSVDKKDPTGAKVTKAAVK 445
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 99 bits (238), Expect = 2e-21
Identities = 46/87 (52%), Positives = 62/87 (71%), Gaps = 1/87 (1%)
Frame = -2
Query: 533 HCPH-CLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 357
H H ++ SEI K+D RTG E PK +K+ +AAI+N+ P+KP+ VE++ +PPLGR
Sbjct: 18 HTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTPTKPMVVEAYSAYPPLGR 77
Query: 356 FAVRDMRQTVAVGVIKAVNFKEAGGGK 276
FA+RDMRQTV VGVIK+V K+ G K
Sbjct: 78 FAIRDMRQTVGVGVIKSVVKKDPSGAK 104
Score = 53.2 bits (122), Expect = 2e-07
Identities = 21/26 (80%), Positives = 23/26 (88%)
Frame = -3
Query: 586 LNHPGQISNGYTPVLDCHTAHIACKF 509
+NH GQI NGYTPVLDCHT+HIA KF
Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKF 26
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 55.2 bits (127), Expect = 5e-08
Identities = 23/68 (33%), Positives = 41/68 (60%)
Frame = -2
Query: 515 QISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMR 336
+I E+K ++D +T K + +K+G A + + + +C+E F +FP LGRF +R
Sbjct: 455 EIIELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTEG 514
Query: 335 QTVAVGVI 312
+T+AVG +
Sbjct: 515 KTIAVGKV 522
Score = 32.3 bits (70), Expect = 0.38
Identities = 20/92 (21%), Positives = 40/92 (43%), Gaps = 2/92 (2%)
Frame = -3
Query: 781 PRQHHY*VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFT 602
P + V ++ + ++ A PG+N+ + + +++ G+V S NP +F
Sbjct: 365 PNKEQVKVVAIYCDEDKVKRAGPGENLRVRITGIEDEDILSGFVL-SSIVNPVPAVTEFV 423
Query: 601 AQVIVLN--HPGQISNGYTPVLDCHTAHIACK 512
AQ+ +L + GY +L H C+
Sbjct: 424 AQLQILELLDNAIFTAGYKAILHIHAVVEECE 455
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 48.4 bits (110), Expect = 5e-06
Identities = 21/77 (27%), Positives = 43/77 (55%)
Frame = -2
Query: 533 HCPHCLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 354
H + ++ +D +TG+ T+ +P+ + + +A++ + P+CVE+F E LGR
Sbjct: 587 HAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRALGRV 646
Query: 353 AVRDMRQTVAVGVIKAV 303
+R +TVA+G + +
Sbjct: 647 FLRSSGRTVAMGKVTRI 663
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 37.1 bits (82), Expect = 0.013
Identities = 25/88 (28%), Positives = 38/88 (43%), Gaps = 3/88 (3%)
Frame = -3
Query: 766 Y*VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIV 587
Y V VEM + L EA+ GDNVG ++ + +++RG V + P + V+
Sbjct: 331 YTVTGVEMFQKILDEALAGDNVGLLLRGIQKADIQRGMVLAKPGSITPHTKFEAIIYVLK 390
Query: 586 LNHPGQIS---NGYTPVLDCHTAHIACK 512
G+ S GY P T + K
Sbjct: 391 KEEGGRHSPFFAGYRPQFYMRTTDVTGK 418
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 33.1 bits (72), Expect = 0.21
Identities = 28/91 (30%), Positives = 40/91 (43%), Gaps = 6/91 (6%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL- 584
V VEM + L GDNVG ++ + ++++RG V +K K F A++ VL
Sbjct: 316 VTGVEMFKKILDNGQAGDNVGLLLRGLKREDIQRGMVI--AKPGSCKTYKKFEAEIYVLT 373
Query: 583 -----NHPGQISNGYTPVLDCHTAHIACKFQ 506
H SN Y P TA I K +
Sbjct: 374 KDEGGRHTAFFSN-YRPQFYLRTADITGKVE 403
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.50
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 455 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 363
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.50
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 455 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 363
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At1g46840.1 68414.m05218 F-box family protein contains F-box domain
Pfam:PF00646; contains 1 predicted transmembrane domain;
similar to Probable disease resistance RPP8-like protein
2 (Swiss-Prot:Q9MAG6) [Arabidopsis thaliana]
Length = 475
Score = 31.9 bits (69), Expect = 0.50
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = -1
Query: 603 QLKSLCLTILVKSQTVTHQSWIATLPTLPANFRN 502
Q K LC+T++ K QT +++ + TL T P +RN
Sbjct: 182 QFKVLCMTVVNKQQTTSYKYQVLTLGTGPLLWRN 215
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 28.7 bits (61), Expect = 4.6
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -3
Query: 133 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 35
I+ K C+ V LFY ++ N S FCY C+L+C
Sbjct: 619 IYLKPCHIFKVGLFYKEVEIARNDGNSRLFCYICRLRC 656
>At4g03430.1 68417.m00470 pre-mRNA splicing factor-related similar
to pre-mRNA splicing factor pre-mRNA splicing factor
prp1 (SP:Q12381) [Fission yeast]
Length = 1029
Score = 28.3 bits (60), Expect = 6.1
Identities = 11/23 (47%), Positives = 17/23 (73%), Gaps = 4/23 (17%)
Frame = -2
Query: 542 GLPHCPHC----LQISEIKEKVD 486
GL HCPHC L +++++EKV+
Sbjct: 820 GLKHCPHCIPLWLSLADLEEKVN 842
>At5g42860.1 68418.m05224 expressed protein
Length = 320
Score = 25.0 bits (52), Expect(2) = 7.2
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -3
Query: 256 SHQGQEVARAVNSTIFHTTAILHSPKGVSKEKRATNS 146
SH G++ A T FH+T +L SP G +++S
Sbjct: 35 SHDGEKTA-----TSFHSTPVLTSPMGSPPHSHSSSS 66
Score = 21.4 bits (43), Expect(2) = 7.2
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -3
Query: 406 PSLYV*SPSRNSH 368
P+ +V SPSR+SH
Sbjct: 24 PAYFVQSPSRDSH 36
>At1g55380.1 68414.m06334 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 661
Score = 27.9 bits (59), Expect = 8.1
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -3
Query: 133 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 35
I+ K C+ V L+Y ++ N S FCY C+L+C
Sbjct: 579 IYLKPCHIFKVGLYYKEVEIARNDGNSRLFCYTCELRC 616
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,803,927
Number of Sequences: 28952
Number of extensions: 375566
Number of successful extensions: 1080
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1038
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1080
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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