Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0566.Seq
(683 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 138 2e-33
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 138 2e-33
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 138 2e-33
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.71
At1g29760.1 68414.m03639 expressed protein 31 0.71
At5g07660.1 68418.m00877 structural maintenance of chromosomes (... 29 2.9
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.9
At1g48490.1 68414.m05420 protein kinase, putative similar to inc... 29 2.9
At5g61460.1 68418.m07712 structural maintenance of chromosomes (... 28 6.6
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 8.8
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 8.8
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 8.8
At1g14210.1 68414.m01682 ribonuclease T2 family protein contains... 27 8.8
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 138 bits (335), Expect = 2e-33
Identities = 63/83 (75%), Positives = 71/83 (85%)
Frame = +1
Query: 1 AXGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
A G KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPA 249
+ I+ QR I+VDGKVRTD TYPA
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPA 84
Score = 127 bits (307), Expect = 6e-30
Identities = 58/95 (61%), Positives = 71/95 (74%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSLGTCV 539
IRYPDPLIK ND+I+LD+ KI++ +G V
Sbjct: 147 IRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVV 181
Score = 58.8 bits (136), Expect = 3e-09
Identities = 25/40 (62%), Positives = 31/40 (77%)
Frame = +1
Query: 562 GACGHIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIG 681
G G I +RE+H GSF+ +HI+DSTGH FATRL NV+ IG
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIG 229
Score = 39.5 bits (88), Expect = 0.002
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVG 574
FIKF+ GN+ M+TGGRN GRVG
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVG 193
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 138 bits (335), Expect = 2e-33
Identities = 63/83 (75%), Positives = 71/83 (85%)
Frame = +1
Query: 1 AXGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
A G KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPA 249
+ I+ QR I+VDGKVRTD TYPA
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPA 84
Score = 127 bits (306), Expect = 8e-30
Identities = 58/95 (61%), Positives = 71/95 (74%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSLGTCV 539
IRYPDPLIK ND+I+LD+ KI++ +G V
Sbjct: 147 IRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181
Score = 58.8 bits (136), Expect = 3e-09
Identities = 25/40 (62%), Positives = 31/40 (77%)
Frame = +1
Query: 562 GACGHIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIG 681
G G I +RE+H GSF+ +HI+DSTGH FATRL NV+ IG
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIG 229
Score = 39.5 bits (88), Expect = 0.002
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVG 574
FIKF+ GN+ M+TGGRN GRVG
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVG 193
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 138 bits (335), Expect = 2e-33
Identities = 63/83 (75%), Positives = 71/83 (85%)
Frame = +1
Query: 1 AXGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
A G KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPA 249
+ I+ QR I+VDGKVRTD TYPA
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPA 84
Score = 127 bits (306), Expect = 8e-30
Identities = 58/95 (61%), Positives = 71/95 (74%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSLGTCV 539
IRYPDPLIK ND+I+LD+ KI++ +G V
Sbjct: 147 IRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181
Score = 58.8 bits (136), Expect = 3e-09
Identities = 25/40 (62%), Positives = 31/40 (77%)
Frame = +1
Query: 562 GACGHIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIG 681
G G I +RE+H GSF+ +HI+DSTGH FATRL NV+ IG
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIG 229
Score = 39.5 bits (88), Expect = 0.002
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +2
Query: 509 FIKFESGNLCMITGGRNLGRVG 574
FIKF+ GN+ M+TGGRN GRVG
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVG 193
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.71
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 122 KHSRNLWGPVDGLGAYTPPSLSNIHAL 42
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.71
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -1
Query: 233 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 141
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At5g07660.1 68418.m00877 structural maintenance of chromosomes
(SMC) family protein similar to SMC-like protein (MIM)
[Arabidopsis thaliana] GI:5880614; contains Pfam profile
PF02463: RecF/RecN/SMC N terminal domain
Length = 1058
Score = 29.1 bits (62), Expect = 2.9
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = +1
Query: 532 LVYDHGRP*LGACGHIVSRERHPGSFDIVHIKDST 636
++YD RP L H++ + HP ++H +++T
Sbjct: 533 IIYDFSRPRLDIPRHMIPQTEHPTILSVLHSENTT 567
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -1
Query: 212 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 96
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At1g48490.1 68414.m05420 protein kinase, putative similar to
incomplete root hair elongation (IRE) [Arabidopsis
thaliana] gi|6729346|dbj|BAA89783
Length = 878
Score = 29.1 bits (62), Expect = 2.9
Identities = 19/72 (26%), Positives = 35/72 (48%), Gaps = 4/72 (5%)
Frame = +3
Query: 444 PDPLIKVNDSIQLDIATTKIMDSSSLSLGT--CV*SREAVTWGVWAHR--VPRETSRLLR 611
P ++K +I D+A K+ + SS +R ++WG W H+ + E+S + R
Sbjct: 137 PPQVVKKLVAISDDMAVAKLPEVSSAKASQEHMSKNRHNISWGYWGHQSCISEESSIICR 196
Query: 612 HCAHQGLHGTHL 647
C + + TH+
Sbjct: 197 ICEEE-IPTTHV 207
>At5g61460.1 68418.m07712 structural maintenance of chromosomes
(SMC) family protein very strong similarity to SMC-like
protein (MIM) [Arabidopsis thaliana] GI:5880614;
contains Pfam profile PF02463: RecF/RecN/SMC N terminal
domain
Length = 1057
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = +1
Query: 532 LVYDHGRP*LGACGHIVSRERHPGSFDIVHIKDST 636
++YD RP L H+V + HP F ++ + T
Sbjct: 532 IIYDFSRPRLNIPRHMVPQTEHPTIFSVIDSDNPT 566
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 8.8
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 8.8
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 27.5 bits (58), Expect = 8.8
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736
>At1g14210.1 68414.m01682 ribonuclease T2 family protein contains
similarity to RNase GI:7768564 from [Nicotiana tabacum];
contains Pfam profile PF00445: Ribonuclease T2 family
Length = 247
Score = 27.5 bits (58), Expect = 8.8
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = +3
Query: 495 TKIMDSSSLSLGTCV*SREAVTWGVWAHRVPRETSRLLRHCAHQGLHGTHLRHEVEQRVH 674
T + D S +S C ++ WGVWA P + L H HGT ++ +Q +
Sbjct: 79 TNLFDISKISDLVCQMEKKWTEWGVWA--CPSNETNLWEH--EWNKHGTCVQSIFDQHSY 134
Query: 675 NR 680
R
Sbjct: 135 FR 136
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,617,705
Number of Sequences: 28952
Number of extensions: 371764
Number of successful extensions: 1066
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1010
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1066
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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