BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0564.Seq (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 48 9e-07 SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 42 1e-04 SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 34 0.020 SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 29 0.77 SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomy... 28 1.0 SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 27 1.8 SPBC1348.05 |||membrane transporter|Schizosaccharomyces pombe|ch... 27 2.3 SPBPB2B2.16c |||MFS family membrane transporter |Schizosaccharom... 27 2.3 SPAC750.02c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 2.3 SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|... 27 3.1 SPBC651.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 4.1 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.1 SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 26 5.4 SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 25 7.2 SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe... 25 7.2 SPCC16A11.01 ||SPCC63.15|conserved fungal protein|Schizosaccharo... 25 9.5 SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 25 9.5 SPBC1683.08 |ght4||hexose transporter Ght4 |Schizosaccharomyces ... 25 9.5 >SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 48.4 bits (110), Expect = 9e-07 Identities = 17/61 (27%), Positives = 29/61 (47%) Frame = +2 Query: 59 YCEFCFEQYIAPACDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLEDGLPY 238 +C+ C++ A C KC I G + +H +C+ C C L G+ +F+ + P Sbjct: 365 WCQTCYDNKYAVKCKKCRKPILGISVKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPI 424 Query: 239 C 241 C Sbjct: 425 C 425 Score = 44.0 bits (99), Expect = 2e-05 Identities = 18/52 (34%), Positives = 27/52 (51%) Frame = +3 Query: 273 CFACXFPVEAGDRWVDALNNNYHSQCFNCTVCKKNLEGQSFFAKGGRPFCKI 428 C +C + AG R + A H QCF C C +NLE F+ + G+ +C + Sbjct: 258 CHSCGGSLRAG-RIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +2 Query: 95 ACDKCHAKIK-GDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLEDGLPYC 241 +C C ++ G ++A GK HP+CF C C + + F+ +G YC Sbjct: 257 SCHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYC 306 Score = 39.5 bits (88), Expect = 4e-04 Identities = 17/66 (25%), Positives = 28/66 (42%), Gaps = 1/66 (1%) Frame = +2 Query: 50 GQLYCEFCFEQYIAPACDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFG-NNPFFLED 226 G+ YC + + +P C C I+ ++ FH C C ++F N P D Sbjct: 302 GKFYCHLDYHEQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRD 361 Query: 227 GLPYCE 244 L +C+ Sbjct: 362 DLYWCQ 367 Score = 35.9 bits (79), Expect = 0.005 Identities = 16/55 (29%), Positives = 23/55 (41%) Frame = +3 Query: 261 FTTKCFACXFPVEAGDRWVDALNNNYHSQCFNCTVCKKNLEGQSFFAKGGRPFCK 425 + KC C P+ V + YHSQC+ C C L + +F P C+ Sbjct: 374 YAVKCKKCRKPILGIS--VKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPICR 426 >SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1150 Score = 41.5 bits (93), Expect = 1e-04 Identities = 20/61 (32%), Positives = 30/61 (49%), Gaps = 1/61 (1%) Frame = +2 Query: 62 CEFCFEQYIAPACDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLE-DGLPY 238 CE + + + C C ++G + A+ K FH E F C C +FG N + E +G Y Sbjct: 168 CETDYFRRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVY 227 Query: 239 C 241 C Sbjct: 228 C 228 Score = 36.7 bits (81), Expect = 0.003 Identities = 15/43 (34%), Positives = 21/43 (48%) Frame = +2 Query: 98 CDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLED 226 C C I G + A+G +H ECF C C L + F ++D Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDD 158 Score = 31.1 bits (67), Expect = 0.14 Identities = 18/52 (34%), Positives = 28/52 (53%) Frame = +3 Query: 243 KQLEELFTTKCFACXFPVEAGDRWVDALNNNYHSQCFNCTVCKKNLEGQSFF 398 K + ++ ++K A V +G ++V AL N YH +CF C C +L FF Sbjct: 105 KAVRKVSSSKICASCGQVISG-QYVRALGNIYHLECFRCHDC-NSLVASKFF 154 Score = 29.9 bits (64), Expect = 0.33 Identities = 13/54 (24%), Positives = 25/54 (46%), Gaps = 1/54 (1%) Frame = +3 Query: 273 CFACXFPVEAGDRWVDALNNNYHSQCFNCTVCKKNL-EGQSFFAKGGRPFCKIH 431 C +C + ++ ALN +H + F C++C S++ G+ +C H Sbjct: 180 CASCGMALRG--YYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYH 231 Score = 25.0 bits (52), Expect = 9.5 Identities = 9/30 (30%), Positives = 14/30 (46%) Frame = +2 Query: 98 CDKCHAKIKGDCLNAIGKHFHPECFNCVYC 187 C CH+ ++ DC+ H C +C C Sbjct: 485 CYVCHSALEEDCVLLGEIRCHIGCLSCTKC 514 >SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 33.9 bits (74), Expect = 0.020 Identities = 17/56 (30%), Positives = 23/56 (41%), Gaps = 3/56 (5%) Frame = +3 Query: 264 TTKCFACXFPVEAGDRWVDALNNNYHSQCFNCTVCKKNLEGQSFF---AKGGRPFC 422 +T CF C + + + + +H CF CT C K LE GRP C Sbjct: 16 STVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVC 71 Score = 31.1 bits (67), Expect = 0.14 Identities = 18/65 (27%), Positives = 28/65 (43%) Frame = +2 Query: 47 NGQLYCEFCFEQYIAPACDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLED 226 +G+ C C A C C +IK L + +H ECF C C K ++ F ++ Sbjct: 66 DGRPVCSSC-----AHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDN 120 Query: 227 GLPYC 241 +C Sbjct: 121 RTIFC 125 Score = 29.9 bits (64), Expect = 0.33 Identities = 14/50 (28%), Positives = 24/50 (48%) Frame = +3 Query: 273 CFACXFPVEAGDRWVDALNNNYHSQCFNCTVCKKNLEGQSFFAKGGRPFC 422 C AC ++ D + + ++YH +CF C C+K + +F FC Sbjct: 78 CTACRMRIK--DYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFC 125 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/37 (32%), Positives = 17/37 (45%), Gaps = 3/37 (8%) Frame = +2 Query: 146 GKHFHPECFNCVYCGKLFGNNPFFL---EDGLPYCEA 247 G +H +CF C C K ++ L DG P C + Sbjct: 37 GHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSS 73 >SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1375 Score = 28.7 bits (61), Expect = 0.77 Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 2/36 (5%) Frame = +2 Query: 50 GQLYCEFCFEQYI--APACDKCHAKIKGDCLNAIGK 151 G LYC FC E ++ + +C C K+ + IG+ Sbjct: 1108 GHLYCSFCLEAWLKHSSSCPMCKTKLNKNNAYYIGE 1143 >SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomyces pombe|chr 1|||Manual Length = 613 Score = 28.3 bits (60), Expect = 1.0 Identities = 11/25 (44%), Positives = 14/25 (56%) Frame = +3 Query: 327 NNNYHSQCFNCTVCKKNLEGQSFFA 401 N+ Y QCF C V K+ LE + A Sbjct: 551 NDTYFGQCFACAVVKRTLERNNITA 575 >SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosaccharomyces pombe|chr 2|||Manual Length = 933 Score = 27.5 bits (58), Expect = 1.8 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = +2 Query: 146 GKHFHPECFNCVYCGK 193 GK +H +CF CV C K Sbjct: 41 GKCWHSDCFKCVNCNK 56 Score = 27.5 bits (58), Expect = 1.8 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 3/34 (8%) Frame = +3 Query: 336 YHSQCFNCTVCKKNLEGQS-FFAKGGRP--FCKI 428 +HS CF C C K L+ S F++ + FCK+ Sbjct: 44 WHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKL 77 >SPBC1348.05 |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = +1 Query: 361 RCVKRTSKDRASSPREVGLSVKYTPVKXPIKIIYNSDTVYSTTTF 495 +C + K S+P E KY P PI++ + V S+ TF Sbjct: 9 KCDSSSIKHVPSTPLETNCPDKYNPKTWPIRLKVRNVIVISSMTF 53 >SPBPB2B2.16c |||MFS family membrane transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = +1 Query: 361 RCVKRTSKDRASSPREVGLSVKYTPVKXPIKIIYNSDTVYSTTTF 495 +C + K S+P E KY P PI++ + V S+ TF Sbjct: 9 KCDSSSIKHVPSTPLETNCPDKYNPKTWPIRLKVRNVIVISSMTF 53 >SPAC750.02c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = +1 Query: 361 RCVKRTSKDRASSPREVGLSVKYTPVKXPIKIIYNSDTVYSTTTF 495 +C + K S+P E KY P PI++ + V S+ TF Sbjct: 9 KCDSSSIKHVPSTPLETNCPDKYNPKTWPIRLKVRNVIVISSMTF 53 >SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 497 Score = 26.6 bits (56), Expect = 3.1 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = +3 Query: 495 SSGFALPITPPGFGTPSP 548 SSG ++P PPGFG P Sbjct: 450 SSGASIPSAPPGFGYQQP 467 >SPBC651.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 237 Score = 26.2 bits (55), Expect = 4.1 Identities = 11/31 (35%), Positives = 20/31 (64%) Frame = -1 Query: 413 PTSLGEEALSFEVLFTHRAVEALTVVVIVER 321 P +GE ++S E +FTH VE++ + + +R Sbjct: 104 PLVVGEASVSLEDIFTHGTVESVIPLFMKKR 134 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 4.1 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +3 Query: 138 TPSVNTSIQSASTAFIAESCSETTR 212 TP++++S+Q S AFI S S T+ Sbjct: 332 TPTISSSVQVNSLAFITSSLSAITK 356 >SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1060 Score = 25.8 bits (54), Expect = 5.4 Identities = 13/22 (59%), Positives = 16/22 (72%) Frame = -1 Query: 83 IAQNRIHSRAVRSLLRTPLVLE 18 IAQ+R SRAVR+ L +P V E Sbjct: 884 IAQSRYGSRAVRACLESPDVTE 905 >SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 764 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 2/30 (6%) Frame = -2 Query: 619 SVLRMSE--NSPPSKWRGVSNFRVLCGDGV 536 S+L +SE + PP++W G VLC D V Sbjct: 412 SLLSVSEVTSHPPTEWTGSPLAHVLCSDVV 441 >SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe|chr 1|||Manual Length = 697 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/44 (29%), Positives = 16/44 (36%) Frame = +2 Query: 98 CDKCHAKIKGDCLNAIGKHFHPECFNCVYCGKLFGNNPFFLEDG 229 C H +K + LN G H C + K G N E G Sbjct: 362 CGGAHIWLKREDLNHGGSHKINNCIGQILLAKRLGKNRIIAETG 405 >SPCC16A11.01 ||SPCC63.15|conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 25.0 bits (52), Expect = 9.5 Identities = 15/38 (39%), Positives = 22/38 (57%), Gaps = 1/38 (2%) Frame = +1 Query: 394 SSPREVGLS-VKYTPVKXPIKIIYNSDTVYSTTTFRPV 504 +S E G+S + T + P+ +YN D +TTT RPV Sbjct: 291 ASATEPGVSNIPETRPERPV--VYNMDVSQTTTTTRPV 326 >SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1117 Score = 25.0 bits (52), Expect = 9.5 Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 2/49 (4%) Frame = -1 Query: 347 LTVVVIVERIDPPVAR--LHGESASETFCCKELFQLLRSMAVRLLRRTG 207 + +++I E++DPPVA+ L GE+ F L +M + LLR G Sbjct: 602 IVILMIDEKMDPPVAKSMLKGEADR----LDSAFHLSYNMILNLLRVEG 646 >SPBC1683.08 |ght4||hexose transporter Ght4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 557 Score = 25.0 bits (52), Expect = 9.5 Identities = 20/65 (30%), Positives = 29/65 (44%), Gaps = 3/65 (4%) Frame = -1 Query: 374 LFTHRAVEALTVVV--IVERIDPPVARLHGESASETF-CCKELFQLLRSMAVRLLRRTGC 204 LFT + AL+V+ + PP R S + F C L +M LR+T Sbjct: 118 LFTGLTIGALSVLSPGYQSEVAPPQIRGAVVSTYQLFQTCGTLIAACINMGTHKLRKTAS 177 Query: 203 FRTTF 189 +RT+F Sbjct: 178 WRTSF 182 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,594,821 Number of Sequences: 5004 Number of extensions: 52242 Number of successful extensions: 198 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 180 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 198 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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