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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0556.Seq
         (679 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g64390.2 68418.m08088 KH domain-containing RNA-binding protei...    31   0.53 
At5g64390.1 68418.m08089 KH domain-containing RNA-binding protei...    31   0.53 
At5g63420.1 68418.m07962 metallo-beta-lactamase family protein         30   1.6  
At2g03610.1 68415.m00321 F-box family protein contains F-box dom...    29   2.1  
At3g55040.1 68416.m06112 In2-1 protein, putative similar to In2-...    28   6.5  
At3g31540.1 68416.m04025 hypothetical protein                          27   8.6  
At1g31930.2 68414.m03924 extra-large guanine nucleotide binding ...    27   8.6  
At1g31930.1 68414.m03923 extra-large guanine nucleotide binding ...    27   8.6  

>At5g64390.2 68418.m08088 KH domain-containing RNA-binding protein
           (HEN4) contains similarity to RNA-binding protein;
           identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
           contains Pfam domain PF00013: KH domain; identical to
           cDNA HEN4 (HEN4) GI:28261402
          Length = 824

 Score = 31.5 bits (68), Expect = 0.53
 Identities = 26/94 (27%), Positives = 42/94 (44%)
 Frame = +1

Query: 394 RIRADTAALIHRARSVVPRAKSVAPLDTIYSYSYGEPIRTVSAMTLTLLSFWCPYAASRI 573
           R R D+   +HR   +VPR     P ++    +YG PI T++  TL   S    Y A R 
Sbjct: 359 RERDDSHDSLHRPFEMVPRDAMGMPFESFPRDAYGRPIETMTQETLRGQS--ADYLAHR- 415

Query: 574 ASTIFPSITSQPRSSLDAATSRACTTPVRRPSPT 675
               + ++ + P S   +A+     T   +P P+
Sbjct: 416 ----YSTLDTHPHSFTTSAS--MANTATMKPPPS 443


>At5g64390.1 68418.m08089 KH domain-containing RNA-binding protein
           (HEN4) contains similarity to RNA-binding protein;
           identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
           contains Pfam domain PF00013: KH domain; identical to
           cDNA HEN4 (HEN4) GI:28261402
          Length = 857

 Score = 31.5 bits (68), Expect = 0.53
 Identities = 26/94 (27%), Positives = 42/94 (44%)
 Frame = +1

Query: 394 RIRADTAALIHRARSVVPRAKSVAPLDTIYSYSYGEPIRTVSAMTLTLLSFWCPYAASRI 573
           R R D+   +HR   +VPR     P ++    +YG PI T++  TL   S    Y A R 
Sbjct: 359 RERDDSHDSLHRPFEMVPRDAMGMPFESFPRDAYGRPIETMTQETLRGQS--ADYLAHR- 415

Query: 574 ASTIFPSITSQPRSSLDAATSRACTTPVRRPSPT 675
               + ++ + P S   +A+     T   +P P+
Sbjct: 416 ----YSTLDTHPHSFTTSAS--MANTATMKPPPS 443


>At5g63420.1 68418.m07962 metallo-beta-lactamase family protein 
          Length = 911

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 26/68 (38%), Positives = 31/68 (45%), Gaps = 5/68 (7%)
 Frame = +1

Query: 475 TIYSYSYGEPIRTVSAMTLTLLSFWCPYA-----ASRIASTIFPSITSQPRSSLDAATSR 639
           +IYS     P  T S M        CPY      +SRI ST+  S+TS P S     TS 
Sbjct: 17  SIYS-DVRRPATTPSKMAAFSALSLCPYTFTFRQSSRIKSTVSCSVTSAPAS----GTSS 71

Query: 640 ACTTPVRR 663
           +  TP RR
Sbjct: 72  SSKTPRRR 79


>At2g03610.1 68415.m00321 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 216

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 13/31 (41%), Positives = 16/31 (51%)
 Frame = -2

Query: 303 DSVQLTEHHGWADPDSPT*CGTSGYVTSRVT 211
           DS+   E   W D D P+ CG     TSR+T
Sbjct: 166 DSICFVEDDLWPDFDRPSNCGIFNLATSRIT 196


>At3g55040.1 68416.m06112 In2-1 protein, putative similar to In2-1
           protein, Zea mays, P49248
          Length = 292

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 20/81 (24%), Positives = 35/81 (43%)
 Frame = +1

Query: 319 PALAISAVDDFLRSEATKTFEDETRRIRADTAALIHRARSVVPRAKSVAPLDTIYSYSYG 498
           P LA+S+ D  L S ++  + D  + +R  +     ++R   P    V         S  
Sbjct: 13  PTLALSSRDVSLSSSSSSLYLDR-KILRPGSGRRWCKSRRTEPILAVVESSRVPELDSSS 71

Query: 499 EPIRTVSAMTLTLLSFWCPYA 561
           EP++     T   +S+ CP+A
Sbjct: 72  EPVQVFDGSTRLYISYTCPFA 92


>At3g31540.1 68416.m04025 hypothetical protein
          Length = 699

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 22/70 (31%), Positives = 29/70 (41%), Gaps = 1/70 (1%)
 Frame = +1

Query: 331 ISAVDDFLRSEATKTFEDETRRIRADT-AALIHRARSVVPRAKSVAPLDTIYSYSYGEPI 507
           I  V D  R  A K    E RR+  ++ AA   RAR VVP   + A  D +       P 
Sbjct: 368 IREVYDKKRLAAEKKRAAEERRVMEESGAAEPSRAREVVPEVAAPAAPDVVDQVPADPPT 427

Query: 508 RTVSAMTLTL 537
            T +   + L
Sbjct: 428 ETATQAVIAL 437


>At1g31930.2 68414.m03924 extra-large guanine nucleotide binding
           protein, putative / G-protein, putative similar to
           extra-large G-protein (XLG) [Arabidopsis thaliana]
           GI:3201680; contains Pfam profile PF00503: G-protein
           alpha subunit
          Length = 848

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +1

Query: 313 RRPALAISAVDDFLRSEATKTFEDETRRIRADT 411
           RRP +    VDD  R E  +  E+E   + A+T
Sbjct: 120 RRPVVKFKPVDDHDRIEGREAAEEEDNNVEAET 152


>At1g31930.1 68414.m03923 extra-large guanine nucleotide binding
           protein, putative / G-protein, putative similar to
           extra-large G-protein (XLG) [Arabidopsis thaliana]
           GI:3201680; contains Pfam profile PF00503: G-protein
           alpha subunit
          Length = 848

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +1

Query: 313 RRPALAISAVDDFLRSEATKTFEDETRRIRADT 411
           RRP +    VDD  R E  +  E+E   + A+T
Sbjct: 120 RRPVVKFKPVDDHDRIEGREAAEEEDNNVEAET 152


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,647,600
Number of Sequences: 28952
Number of extensions: 308466
Number of successful extensions: 869
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 845
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 868
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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