Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0539.Seq
(663 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g08580.1 68418.m01021 calcium-binding EF hand family protein ... 36 0.032
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct... 36 0.032
At5g39420.1 68418.m04775 protein kinase family protein contains ... 31 0.68
At5g53970.1 68418.m06714 aminotransferase, putative similar to n... 31 0.90
At4g21450.2 68417.m03102 vesicle-associated membrane family prot... 30 1.2
At4g21450.1 68417.m03103 vesicle-associated membrane family prot... 30 1.2
At4g11720.1 68417.m01870 hypothetical protein 29 2.8
At1g43720.1 68414.m05023 hypothetical protein 29 2.8
At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64... 29 3.6
At5g61910.3 68418.m07772 expressed protein 28 4.8
At5g61910.2 68418.m07771 expressed protein 28 4.8
At5g61910.1 68418.m07770 expressed protein 28 4.8
At4g14500.1 68417.m02235 expressed protein weak similarity to SP... 28 4.8
At2g30230.1 68415.m03678 hypothetical protein 28 4.8
At1g73360.1 68414.m08491 homeobox-leucine zipper family protein ... 28 4.8
At5g40520.1 68418.m04916 expressed protein 28 6.4
At2g25870.1 68415.m03105 haloacid dehalogenase-like hydrolase fa... 28 6.4
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 28 6.4
At5g47800.1 68418.m05904 phototropic-responsive NPH3 family prot... 27 8.4
At5g27710.1 68418.m03324 expressed protein 27 8.4
At5g10020.1 68418.m01161 leucine-rich repeat transmembrane prote... 27 8.4
At4g27860.1 68417.m04000 integral membrane family protein contai... 27 8.4
>At5g08580.1 68418.m01021 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 391
Score = 35.5 bits (78), Expect = 0.032
Identities = 19/84 (22%), Positives = 39/84 (46%), Gaps = 1/84 (1%)
Frame = -1
Query: 489 RYHEPEAHAAQTLQQSQHDNEDDVPIILDAEHRYHEDEHQRRLSAHHH-ELGDDVREQDL 313
++H + ++ + ++HD P++ D E R + E +R+ H H EL +++
Sbjct: 39 QHHRLKLRSSFNFKPTRHDPVPFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETT 98
Query: 312 SGRHSRHPGSVQETLHPFHVNADD 241
G H PG + ++A+D
Sbjct: 99 GGGHEHAPGHESQPEWEEFMDAED 122
>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
PIR2:A28490
Length = 725
Score = 35.5 bits (78), Expect = 0.032
Identities = 13/53 (24%), Positives = 28/53 (52%)
Frame = -1
Query: 474 EAHAAQTLQQSQHDNEDDVPIILDAEHRYHEDEHQRRLSAHHHELGDDVREQD 316
E + LQ+ + +N+D VP++ + + H DE + ++ + D V+ +D
Sbjct: 68 EVKEEEQLQEPKEENQDSVPLVEEVQDPIHADESENKICSVDQPTDDQVKPED 120
>At5g39420.1 68418.m04775 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 644
Score = 31.1 bits (67), Expect = 0.68
Identities = 25/88 (28%), Positives = 37/88 (42%), Gaps = 2/88 (2%)
Frame = +3
Query: 345 RGDVRTDGAGAHLHDIGVRRQE*WEHRLRYH-ADFAARSVR-HVLRVRDIGRLRARANAI 518
R D+R A L D GV R+ HR Y ++A +R L V++I +RA
Sbjct: 415 RDDMRRKRANLKLRDSGVGRKHKRPHRAEYDPKNYAKLPIRKDTLEVKNIPNEASRATT- 473
Query: 519 QLALGSFYPTLLLSGVIWPIEGMPWILR 602
G++Y L P G W ++
Sbjct: 474 -TTHGNYYKVSDLPMTTGPASGFAWAVK 500
>At5g53970.1 68418.m06714 aminotransferase, putative similar to
nicotianamine aminotransferase from Hordeum vulgare
[GI:6498122, GI:6469087]; contains Pfam profile PF00155
aminotransferase, classes I and II
Length = 414
Score = 30.7 bits (66), Expect = 0.90
Identities = 23/70 (32%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +2
Query: 89 LLETCDYNPKLGDIPIDFMDPIYGNKNPSFTDFVAPGVILTIVFFLAVALTSSALTWNGW 268
L++ + KLG + I D +YG+ FV GV +IV L + S GW
Sbjct: 193 LMKIAESAKKLGFLVI--ADEVYGHLAFGSKPFVPMGVFGSIVPVLTLGSLSKRWIVPGW 250
Query: 269 RVSW---TDP 289
R+ W TDP
Sbjct: 251 RLGWFVTTDP 260
>At4g21450.2 68417.m03102 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 212
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = -1
Query: 396 HRYHEDEHQRRLSAHHHELGDDVREQDLSGRHSRHP 289
H +H+ HQ HHH+LG + D SG+ ++HP
Sbjct: 55 HNHHQHHHQHH-HQHHHQLGYNGPHGDGSGQ-NQHP 88
>At4g21450.1 68417.m03103 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 295
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = -1
Query: 396 HRYHEDEHQRRLSAHHHELGDDVREQDLSGRHSRHP 289
H +H+ HQ HHH+LG + D SG+ ++HP
Sbjct: 55 HNHHQHHHQHH-HQHHHQLGYNGPHGDGSGQ-NQHP 88
>At4g11720.1 68417.m01870 hypothetical protein
Length = 658
Score = 29.1 bits (62), Expect = 2.8
Identities = 17/66 (25%), Positives = 30/66 (45%), Gaps = 2/66 (3%)
Frame = -1
Query: 399 EHRYHEDEHQR--RLSAHHHELGDDVREQDLSGRHSRHPGSVQETLHPFHVNADDVRATA 226
+HR+H + H+R + HHH DDV ++ + R LH H ++ +
Sbjct: 576 KHRHHHNHHRRTHQRHKHHHGQDDDVLQKMMLER-DHSDSHYYHQLHRVHKDSKQKQRRR 634
Query: 225 RKNTMV 208
K+ +V
Sbjct: 635 AKHGIV 640
>At1g43720.1 68414.m05023 hypothetical protein
Length = 314
Score = 29.1 bits (62), Expect = 2.8
Identities = 17/70 (24%), Positives = 27/70 (38%), Gaps = 3/70 (4%)
Frame = -1
Query: 387 HEDEHQRRLSAHHH---ELGDDVREQDLSGRHSRHPGSVQETLHPFHVNADDVRATARKN 217
H +R H H E G+D + D QE + V DD +TAR+
Sbjct: 16 HSARQRREQLIHEHGVDEEGEDYSDSDSGNMPEAEVPETQEEEEVYRVTIDDDESTARRG 75
Query: 216 TMVNMTPGAT 187
+ + G++
Sbjct: 76 SSAQRSGGSS 85
>At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194
Lysosomal acid lipase/cholesteryl ester hydrolase
precursor (EC 3.1.1.13) {Rattus norvegicus}; contains
Pfam profile PF04083: ab-hydrolase associated lipase
region
Length = 418
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +3
Query: 504 RANAIQLALGSFYPTLLLSGVIWPIEGMPWILRYVSLCLPLTLA 635
RA A+ G P L+ G++ ++GM W+L LPL LA
Sbjct: 85 RAGAVAGDGGKRQPVLIQHGIL--VDGMSWLLNPADQNLPLILA 126
>At5g61910.3 68418.m07772 expressed protein
Length = 742
Score = 28.3 bits (60), Expect = 4.8
Identities = 19/85 (22%), Positives = 33/85 (38%), Gaps = 4/85 (4%)
Frame = -1
Query: 501 LASGRYHEPEAHAAQTLQQSQHDNEDDVPIILDAEHR--YHEDEHQRRLSAHHHELGDDV 328
+ +G YH PEA +S + V + Y H +G
Sbjct: 404 IPAGTYHHPEASTVSNTTKSMQPDMQAVSVAQSHTETAGYPTPAHGEASQPPAGAIGYTH 463
Query: 327 REQDLSGRHSRH--PGSVQETLHPF 259
+ Q ++G +S H PG+V+E+ +
Sbjct: 464 QPQSVAGNYSTHSQPGNVEESTQSY 488
>At5g61910.2 68418.m07771 expressed protein
Length = 738
Score = 28.3 bits (60), Expect = 4.8
Identities = 19/85 (22%), Positives = 33/85 (38%), Gaps = 4/85 (4%)
Frame = -1
Query: 501 LASGRYHEPEAHAAQTLQQSQHDNEDDVPIILDAEHR--YHEDEHQRRLSAHHHELGDDV 328
+ +G YH PEA +S + V + Y H +G
Sbjct: 400 IPAGTYHHPEASTVSNTTKSMQPDMQAVSVAQSHTETAGYPTPAHGEASQPPAGAIGYTH 459
Query: 327 REQDLSGRHSRH--PGSVQETLHPF 259
+ Q ++G +S H PG+V+E+ +
Sbjct: 460 QPQSVAGNYSTHSQPGNVEESTQSY 484
>At5g61910.1 68418.m07770 expressed protein
Length = 738
Score = 28.3 bits (60), Expect = 4.8
Identities = 19/85 (22%), Positives = 33/85 (38%), Gaps = 4/85 (4%)
Frame = -1
Query: 501 LASGRYHEPEAHAAQTLQQSQHDNEDDVPIILDAEHR--YHEDEHQRRLSAHHHELGDDV 328
+ +G YH PEA +S + V + Y H +G
Sbjct: 400 IPAGTYHHPEASTVSNTTKSMQPDMQAVSVAQSHTETAGYPTPAHGEASQPPAGAIGYTH 459
Query: 327 REQDLSGRHSRH--PGSVQETLHPF 259
+ Q ++G +S H PG+V+E+ +
Sbjct: 460 QPQSVAGNYSTHSQPGNVEESTQSY 484
>At4g14500.1 68417.m02235 expressed protein weak similarity to
SP|Q9UKL6 Phosphatidylcholine transfer protein (PC-TP)
{Homo sapiens}
Length = 433
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +2
Query: 164 KNPSFTDFVAPGVILTIVFFLAVALTSSALTWNGWRVSWT 283
KNPSFT+ ++ + +LAV + + W+ WR WT
Sbjct: 13 KNPSFTETFVDILLCAVPIWLAV-MIGLLIGWS-WRPRWT 50
>At2g30230.1 68415.m03678 hypothetical protein
Length = 177
Score = 28.3 bits (60), Expect = 4.8
Identities = 17/59 (28%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = -2
Query: 527 GQLDRVRSSSQAADI--TNPKHMPHRPCSKVSMITKTMFPLFLTPNTDIMKMSTSAVCP 357
G +D + S A +I NP H+ +PCS+ ++ K L L+P +++ + S + P
Sbjct: 28 GHVDEITSPMTAGEILQANPNHVLSKPCSQ-GVVRKI---LILSPESELKRGSIYFLIP 82
>At1g73360.1 68414.m08491 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein
protodermal factor2 (GI:14276060) [Arabidopsis
thaliana]; similar to homeobox protein GI:1173621 from [
Phalaenopsis sp.]
Length = 722
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/59 (27%), Positives = 30/59 (50%)
Frame = -1
Query: 402 AEHRYHEDEHQRRLSAHHHELGDDVREQDLSGRHSRHPGSVQETLHPFHVNADDVRATA 226
+E Y +++ R +AH E + R ++ ++ P S TLHP H++ D+ T+
Sbjct: 131 SEDPYFDEQKLRIENAHLRE--ELERMSTIASKYMGRPISQLSTLHPMHISPLDLSMTS 187
>At5g40520.1 68418.m04916 expressed protein
Length = 693
Score = 27.9 bits (59), Expect = 6.4
Identities = 20/49 (40%), Positives = 27/49 (55%), Gaps = 4/49 (8%)
Frame = -3
Query: 169 VLIPVYGVHEVNR-DVAQL--RVVVASLQQTLSEVPVRELYV-PVEHKS 35
VL P HE N+ D + RVVV L ++++ V R LYV P +KS
Sbjct: 490 VLDPTDSTHETNQHDFSSSVNRVVVRDLPESITRVYPRSLYVTPTSNKS 538
>At2g25870.1 68415.m03105 haloacid dehalogenase-like hydrolase
family protein contains Pfam profiles PF00702: haloacid
dehalogenase-like hydrolase, PF02130: Uncharacterized
protein family UPF0054
Length = 584
Score = 27.9 bits (59), Expect = 6.4
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = -1
Query: 474 EAHAAQTLQQSQHDNEDDVPIILDAEHRYHEDEHQRRLSAHHHELGDDVR 325
E HA L SQH E +P+++ + + R+ + H L D++R
Sbjct: 186 EDHATDVLSMSQHVPELKLPVLMMGDLVISVETAARQAAERGHTLLDEIR 235
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.9 bits (59), Expect = 6.4
Identities = 18/49 (36%), Positives = 22/49 (44%)
Frame = -1
Query: 432 NEDDVPIILDAEHRYHEDEHQRRLSAHHHELGDDVREQDLSGRHSRHPG 286
NEDD D +H +H D H HHH+ G D S H+ PG
Sbjct: 304 NEDDKGDHHDHDHDHHHD-HNHDHDHHHHD-GHDHHHH--SHDHTHDPG 348
>At5g47800.1 68418.m05904 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 559
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -1
Query: 219 NTMVNMTPGATKSVNEGFL 163
NT+VNM P SV+EGFL
Sbjct: 271 NTVVNMIPADKGSVSEGFL 289
>At5g27710.1 68418.m03324 expressed protein
Length = 380
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = -1
Query: 291 PGSVQETLHPFHVNADDVRATARKNTMVNMTP 196
PGS+Q TL+P + DV + + + TP
Sbjct: 135 PGSIQRTLNPKMIPVPDVERSRKDKCQLTRTP 166
>At5g10020.1 68418.m01161 leucine-rich repeat transmembrane protein
kinase, putative receptor-like protein kinase ERECTA,
Arabidopsis thaliana, EMBL:AC004484
Length = 1048
Score = 27.5 bits (58), Expect = 8.4
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = +1
Query: 604 TCPSACPSPWPRTRCGP 654
T PS CP+ WP C P
Sbjct: 55 TDPSTCPNDWPGISCDP 71
>At4g27860.1 68417.m04000 integral membrane family protein contains
Pfam PF01988: Integral membrane protein
Length = 611
Score = 27.5 bits (58), Expect = 8.4
Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = -1
Query: 519 GSRSL*LASGRYHEPEAHA-AQTLQQSQHDNEDDVPIILDAEHRYH 385
GSRS+ +Y E E A+ LQ +DDV I++ +H+Y+
Sbjct: 60 GSRSV--DKNQYSETEVVVRAKDLQTEPDSLDDDVEIVIKNQHKYY 103
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,402,990
Number of Sequences: 28952
Number of extensions: 300339
Number of successful extensions: 1128
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1070
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1121
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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