Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0538X.Seq
         (499 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct...    36   0.020
At5g08580.1 68418.m01021 calcium-binding EF hand family protein ...    35   0.035
At4g21450.2 68417.m03102 vesicle-associated membrane family prot...    31   0.43 
At4g21450.1 68417.m03103 vesicle-associated membrane family prot...    31   0.43 
At4g11720.1 68417.m01870 hypothetical protein                          28   3.0  
At5g40520.1 68418.m04916 expressed protein                             28   4.0  
At5g10820.1 68418.m01257 integral membrane transporter family pr...    28   4.0  
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim...    28   4.0  
At5g47800.1 68418.m05904 phototropic-responsive NPH3 family prot...    27   5.3  
At2g40650.1 68415.m05016 pre-mRNA splicing factor PRP38 family p...    27   5.3  
At5g48560.1 68418.m06005 basic helix-loop-helix (bHLH) family pr...    27   7.0  
At4g38225.2 68417.m05396 expressed protein                             27   7.0  
At2g25870.1 68415.m03105 haloacid dehalogenase-like hydrolase fa...    27   7.0  
At1g49850.1 68414.m05589 zinc finger (C3HC4-type RING finger) fa...    27   7.0  
At3g29075.1 68416.m03637 glycine-rich protein                          27   9.3  

>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
           RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
           PIR2:A28490
          Length = 725

 Score = 35.5 bits (78), Expect = 0.020
 Identities = 13/53 (24%), Positives = 28/53 (52%)
 Frame = -3

Query: 473 EAHAAQTLQQSQHDNEDDVPMILDAEHRYHEDEHQRRLSAHHHELGDDVREQD 315
           E    + LQ+ + +N+D VP++ + +   H DE + ++ +      D V+ +D
Sbjct: 68  EVKEEEQLQEPKEENQDSVPLVEEVQDPIHADESENKICSVDQPTDDQVKPED 120


>At5g08580.1 68418.m01021 calcium-binding EF hand family protein
           contains INTERPRO:IPR002048 calcium-binding EF-hand
           domain
          Length = 391

 Score = 34.7 bits (76), Expect = 0.035
 Identities = 17/69 (24%), Positives = 33/69 (47%), Gaps = 1/69 (1%)
 Frame = -3

Query: 488 RYHEPEAHAAQTLQQSQHDNEDDVPMILDAEHRYHEDEHQRRLSAHHH-ELGDDVREQDL 312
           ++H  +  ++   + ++HD     P++ D E R  + E +R+   H H EL    +++  
Sbjct: 39  QHHRLKLRSSFNFKPTRHDPVPFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETT 98

Query: 311 SGRHSRHPG 285
            G H   PG
Sbjct: 99  GGGHEHAPG 107


>At4g21450.2 68417.m03102 vesicle-associated membrane family protein
           / VAMP family protein similar to VAP27 GI:6688926
           [Nicotiana plumbaginifolia]
          Length = 212

 Score = 31.1 bits (67), Expect = 0.43
 Identities = 19/53 (35%), Positives = 28/53 (52%)
 Frame = -3

Query: 395 HRYHEDEHQRRLSAHHHELGDDVREQDLSGRHSRHPGSVQETLHPSTSTLTMS 237
           H +H+  HQ     HHH+LG +    D SG+ ++HP     T  PS S++  S
Sbjct: 55  HNHHQHHHQHH-HQHHHQLGYNGPHGDGSGQ-NQHP-----TPSPSVSSVAKS 100


>At4g21450.1 68417.m03103 vesicle-associated membrane family protein
           / VAMP family protein similar to VAP27 GI:6688926
           [Nicotiana plumbaginifolia]
          Length = 295

 Score = 31.1 bits (67), Expect = 0.43
 Identities = 19/53 (35%), Positives = 28/53 (52%)
 Frame = -3

Query: 395 HRYHEDEHQRRLSAHHHELGDDVREQDLSGRHSRHPGSVQETLHPSTSTLTMS 237
           H +H+  HQ     HHH+LG +    D SG+ ++HP     T  PS S++  S
Sbjct: 55  HNHHQHHHQHH-HQHHHQLGYNGPHGDGSGQ-NQHP-----TPSPSVSSVAKS 100


>At4g11720.1 68417.m01870 hypothetical protein
          Length = 658

 Score = 28.3 bits (60), Expect = 3.0
 Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
 Frame = -3

Query: 398 EHRYHEDEHQR--RLSAHHHELGDDVREQDLSGR-HS 297
           +HR+H + H+R  +   HHH   DDV ++ +  R HS
Sbjct: 576 KHRHHHNHHRRTHQRHKHHHGQDDDVLQKMMLERDHS 612


>At5g40520.1 68418.m04916 expressed protein
          Length = 693

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 20/49 (40%), Positives = 27/49 (55%), Gaps = 4/49 (8%)
 Frame = -1

Query: 169 VLIPVYGVHEVNR-DVAQL--RVVVASLQQTLSEVPVRELYV-PVEHKS 35
           VL P    HE N+ D +    RVVV  L ++++ V  R LYV P  +KS
Sbjct: 490 VLDPTDSTHETNQHDFSSSVNRVVVRDLPESITRVYPRSLYVTPTSNKS 538


>At5g10820.1 68418.m01257 integral membrane transporter family
           protein contains 11 transmembrane domains; similar to
           folate/methotrexate transporter FT1 (GI:5813863)
           {Leishmania donovani};
           lignostilbene-alpha,beta-dioxygenase gene, Synechococcus
           PCC7942, EMBL:AF055873
          Length = 503

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
 Frame = +3

Query: 381 FMISVFGVKNH--GNIVFVIMLTLLQGLCGMCFGFVI 485
           F++  FGV  H  GN+  VI++  +  L  +CF F++
Sbjct: 434 FVLHAFGVTRHDFGNLWLVILIRNILRLVTVCFVFLV 470


>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
           similar to PRLI-interacting factor L [Arabidopsis
           thaliana] GI:11139268; contains Pfam profile PF02492:
           Cobalamin synthesis protein/P47K
          Length = 444

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 18/49 (36%), Positives = 22/49 (44%)
 Frame = -3

Query: 431 NEDDVPMILDAEHRYHEDEHQRRLSAHHHELGDDVREQDLSGRHSRHPG 285
           NEDD     D +H +H D H      HHH+ G D      S  H+  PG
Sbjct: 304 NEDDKGDHHDHDHDHHHD-HNHDHDHHHHD-GHDHHHH--SHDHTHDPG 348


>At5g47800.1 68418.m05904 phototropic-responsive NPH3 family protein
           contains NPH3 family domain, Pfam:PF03000
          Length = 559

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 12/19 (63%), Positives = 14/19 (73%)
 Frame = -2

Query: 219 NTMVNMTPGATKSVNEGFL 163
           NT+VNM P    SV+EGFL
Sbjct: 271 NTVVNMIPADKGSVSEGFL 289


>At2g40650.1 68415.m05016 pre-mRNA splicing factor PRP38 family
           protein contains Pfam profile PF03371: PRP38 family
          Length = 355

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 13/46 (28%), Positives = 21/46 (45%)
 Frame = -3

Query: 431 NEDDVPMILDAEHRYHEDEHQRRLSAHHHELGDDVREQDLSGRHSR 294
           +ED++     +  R  E +  RR  +H H   D  R+ D+   H R
Sbjct: 204 SEDEMDQRRKSPERERERDRDRRRDSHRHRDRDYDRDYDMDRDHDR 249


>At5g48560.1 68418.m06005 basic helix-loop-helix (bHLH) family
           protein contains Pfam profile: PF00010 helix-loop-helix
           DNA-binding domain
          Length = 498

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = -3

Query: 413 MILDAEHRYHEDEHQRRLSAHHHE 342
           MI  + +R HE+  Q + S+HHH+
Sbjct: 389 MIPSSNNRLHEEGLQSKSSSHHHQ 412


>At4g38225.2 68417.m05396 expressed protein
          Length = 308

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 10/22 (45%), Positives = 12/22 (54%)
 Frame = +3

Query: 93  WRLATTTRSWATSRLTSWTPYT 158
           WR+   T S+  S   SW PYT
Sbjct: 269 WRVLLATTSFHASDFVSWRPYT 290


>At2g25870.1 68415.m03105 haloacid dehalogenase-like hydrolase
           family protein contains Pfam profiles PF00702: haloacid
           dehalogenase-like hydrolase, PF02130: Uncharacterized
           protein family UPF0054
          Length = 584

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 14/50 (28%), Positives = 24/50 (48%)
 Frame = -3

Query: 473 EAHAAQTLQQSQHDNEDDVPMILDAEHRYHEDEHQRRLSAHHHELGDDVR 324
           E HA   L  SQH  E  +P+++  +     +   R+ +   H L D++R
Sbjct: 186 EDHATDVLSMSQHVPELKLPVLMMGDLVISVETAARQAAERGHTLLDEIR 235


>At1g49850.1 68414.m05589 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile: PF00097 zinc
           finger, C3HC4 type (RING finger)
          Length = 250

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 9/24 (37%), Positives = 12/24 (50%)
 Frame = -3

Query: 401 AEHRYHEDEHQRRLSAHHHELGDD 330
           + +R+H   H RR   HHH    D
Sbjct: 27  SSYRHHSHHHHRRHGVHHHNQRHD 50


>At3g29075.1 68416.m03637 glycine-rich protein 
          Length = 294

 Score = 26.6 bits (56), Expect = 9.3
 Identities = 11/42 (26%), Positives = 21/42 (50%)
 Frame = -3

Query: 473 EAHAAQTLQQSQHDNEDDVPMILDAEHRYHEDEHQRRLSAHH 348
           E H  +  ++ +H + DD       +HR  +DE +++   HH
Sbjct: 249 EYHDDEDKKKKKHYDNDDDEKKKKKDHRDDDDEKKKKKDKHH 290


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,084,954
Number of Sequences: 28952
Number of extensions: 229719
Number of successful extensions: 814
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 784
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 808
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)