BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0536.Seq (722 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 148 7e-37 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 147 2e-36 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 124 1e-29 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 107 2e-24 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 79 8e-16 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 66 4e-12 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 64 1e-11 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 60 2e-10 SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 26 4.7 SPAC3G6.05 |||Mvp17/PMP22 family|Schizosaccharomyces pombe|chr 1... 26 4.7 SPAC2C4.15c |ubx2|ucp13|UBX domain protein Ubx2|Schizosaccharomy... 26 6.3 SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 26 6.3 SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces p... 25 8.3 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 148 bits (359), Expect = 7e-37 Identities = 72/84 (85%), Positives = 79/84 (94%) Frame = +3 Query: 3 ACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAK 182 ACERAKRTLSSS QASIEIDSLFEGIDFYTSITRARFEEL ADLFR TMEPVE+ LRD+K Sbjct: 264 ACERAKRTLSSSAQASIEIDSLFEGIDFYTSITRARFEELCADLFRKTMEPVERVLRDSK 323 Query: 183 MDKAQIHDIVLVGGSTRIPKVQKL 254 +DKA +++IVLVGGSTRIP+VQKL Sbjct: 324 VDKASVNEIVLVGGSTRIPRVQKL 347 Score = 102 bits (244), Expect = 6e-23 Identities = 48/53 (90%), Positives = 50/53 (94%) Frame = +1 Query: 508 QIGVLIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILN 666 Q GVLIQVFEGERA TKD NLLGKFEL+GIPPAPRGVPQIEVTFD+DANGILN Sbjct: 433 QPGVLIQVFEGERARTKDCNLLGKFELSGIPPAPRGVPQIEVTFDVDANGILN 485 Score = 74.9 bits (176), Expect = 1e-14 Identities = 54/162 (33%), Positives = 64/162 (39%) Frame = +2 Query: 236 PQGAEALQDFFNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGI 415 P+ + + DFFNGKE KSINPDE +L GD SE+ QD GI Sbjct: 342 PRVQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAVLTGDTSEKTQDLLLLDVAPLSMGI 401 Query: 416 ETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNKSEYSSKYLRVSVL*PKITTCSVNSS*PG 595 ETAGGVMT LIKRN YSDN+ + K G Sbjct: 402 ETAGGVMTPLIKRNTTIPTKKSEIFSTYSDNQPGVLIQVFEGERARTKDCNLLGKFELSG 461 Query: 596 SHRRRVACLKLRXXXXXXXXXXXXXXAIEKSTNKENKITITN 721 ++ A+EK T K KITITN Sbjct: 462 IPPAPRGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITN 503 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 147 bits (356), Expect = 2e-36 Identities = 70/84 (83%), Positives = 80/84 (95%) Frame = +3 Query: 3 ACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAK 182 ACERAKRTLSSS QASIEIDSL+EGIDFYTSITRARFEEL ADLFR+TMEPVEK LRD+K Sbjct: 264 ACERAKRTLSSSAQASIEIDSLYEGIDFYTSITRARFEELCADLFRNTMEPVEKVLRDSK 323 Query: 183 MDKAQIHDIVLVGGSTRIPKVQKL 254 +DK+ +++IVLVGGSTRIP++QKL Sbjct: 324 IDKSSVNEIVLVGGSTRIPRIQKL 347 Score = 102 bits (244), Expect = 6e-23 Identities = 48/53 (90%), Positives = 50/53 (94%) Frame = +1 Query: 508 QIGVLIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILN 666 Q GVLIQVFEGERA TKD NLLGKFEL+GIPPAPRGVPQIEVTFD+DANGILN Sbjct: 433 QPGVLIQVFEGERARTKDCNLLGKFELSGIPPAPRGVPQIEVTFDVDANGILN 485 Score = 73.3 bits (172), Expect = 3e-14 Identities = 54/162 (33%), Positives = 64/162 (39%) Frame = +2 Query: 236 PQGAEALQDFFNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGI 415 P+ + + DFFNGKE KSINPDE IL GD SE+ QD GI Sbjct: 342 PRIQKLVSDFFNGKEPCKSINPDEAVAYGAAVQAAILVGDTSEKTQDLLLLDVAPLSLGI 401 Query: 416 ETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNKSEYSSKYLRVSVL*PKITTCSVNSS*PG 595 ETAGGVMT LIKRN Y+DN+ + K G Sbjct: 402 ETAGGVMTPLIKRNTTIPTKKSEVFSTYADNQPGVLIQVFEGERARTKDCNLLGKFELSG 461 Query: 596 SHRRRVACLKLRXXXXXXXXXXXXXXAIEKSTNKENKITITN 721 ++ A+EK T K KITITN Sbjct: 462 IPPAPRGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITN 503 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 124 bits (299), Expect = 1e-29 Identities = 57/84 (67%), Positives = 72/84 (85%) Frame = +3 Query: 3 ACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAK 182 ACERAKR LSS TQ ++E+DSL GIDF +SITRARFE++NA F++T++PV K L+D+K Sbjct: 268 ACERAKRALSSVTQTTVEVDSLSNGIDFSSSITRARFEDINATTFKATIDPVAKVLKDSK 327 Query: 183 MDKAQIHDIVLVGGSTRIPKVQKL 254 + KA +HDIVLVGGSTRIPKVQ+L Sbjct: 328 VPKADVHDIVLVGGSTRIPKVQRL 351 Score = 64.5 bits (150), Expect = 1e-11 Identities = 28/52 (53%), Positives = 37/52 (71%) Frame = +1 Query: 508 QIGVLIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGIL 663 Q V V++GER +N LG+F+LTGIPP PRG ++E TF++DANGIL Sbjct: 438 QTTVTFPVYQGERVNCAENEPLGEFQLTGIPPMPRGQAELEATFELDANGIL 489 Score = 40.3 bits (90), Expect = 3e-04 Identities = 25/94 (26%), Positives = 38/94 (40%), Gaps = 1/94 (1%) Frame = +2 Query: 236 PQGAEALQDFFNGKELNKSINPDEXXXXXXXXXXXIL-HGDKSEEVQDXXXXXXXXXXXG 412 P+ + DFF+G+ LNKSINPDE +L + S++ QD G Sbjct: 346 PKVQRLVSDFFDGRALNKSINPDEAVAYGAAVQAAVLTNKADSDKTQDLLLLDVVPLSLG 405 Query: 413 IETAGGVMTTLIKRNXXXXXXXXXXXXXYSDNKS 514 + G V + RN +DN++ Sbjct: 406 VAMEGNVFGVVCPRNTPIPTIKKRTFTTVADNQT 439 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 107 bits (257), Expect = 2e-24 Identities = 50/95 (52%), Positives = 72/95 (75%) Frame = +3 Query: 9 ERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMD 188 E+AKRTLSS IEI+S F G DF +++RA+FEE+N DLF+ T++PVE+ L+D+ + Sbjct: 298 EKAKRTLSSQKSVRIEIESFFNGQDFSETLSRAKFEEINMDLFKKTLKPVEQVLKDSNLK 357 Query: 189 KAQIHDIVLVGGSTRIPKVQKLCKISLMERSSTNL 293 K++I DIVLVGGSTRIPKVQ+L + +++S + Sbjct: 358 KSEIDDIVLVGGSTRIPKVQELLESFFGKKASKGI 392 Score = 94.7 bits (225), Expect = 1e-20 Identities = 41/49 (83%), Positives = 47/49 (95%) Frame = +1 Query: 517 VLIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGIL 663 VLIQV+EGER +TKDNNLLGKF+L GIPPAPRGVPQIEVTF++DANG+L Sbjct: 465 VLIQVYEGERTLTKDNNLLGKFDLRGIPPAPRGVPQIEVTFEVDANGVL 513 Score = 46.4 bits (105), Expect = 4e-06 Identities = 28/74 (37%), Positives = 37/74 (50%) Frame = +2 Query: 236 PQGAEALQDFFNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXXXXXXGI 415 P+ E L+ FF GK+ +K INPDE +L G++ + + GI Sbjct: 374 PKVQELLESFF-GKKASKGINPDEAVAYGAAVQAGVLSGEEGSD--NIVLLDVIPLTLGI 430 Query: 416 ETAGGVMTTLIKRN 457 ET GGVMT LI RN Sbjct: 431 ETTGGVMTKLIGRN 444 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 78.6 bits (185), Expect = 8e-16 Identities = 33/53 (62%), Positives = 44/53 (83%) Frame = +1 Query: 508 QIGVLIQVFEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILN 666 Q V I+VF+GER + +DN L+G F+LTGI PAP+G PQIEV+FD+DA+GI+N Sbjct: 476 QTAVEIRVFQGERELVRDNKLIGNFQLTGIAPAPKGQPQIEVSFDVDADGIIN 528 Score = 58.8 bits (136), Expect = 7e-10 Identities = 33/96 (34%), Positives = 59/96 (61%), Gaps = 4/96 (4%) Frame = +3 Query: 3 ACERAKRTLSSSTQASIEIDSLFEGID----FYTSITRARFEELNADLFRSTMEPVEKSL 170 A E+AK LSS ++ I + + I+RA+FE+L L R T++P +++L Sbjct: 308 AAEKAKCELSSLSKTDISLPFITADATGPKHINMEISRAQFEKLVDPLVRRTIDPCKRAL 367 Query: 171 RDAKMDKAQIHDIVLVGGSTRIPKVQKLCKISLMER 278 +DA + ++I++++LVGG TR+P+V + K S+ +R Sbjct: 368 KDANLQTSEINEVILVGGMTRMPRVVETVK-SIFKR 402 Score = 39.9 bits (89), Expect = 4e-04 Identities = 27/80 (33%), Positives = 35/80 (43%) Frame = +2 Query: 218 GWLHSYPQGAEALQDFFNGKELNKSINPDEXXXXXXXXXXXILHGDKSEEVQDXXXXXXX 397 G + P+ E ++ F +E KS+NPDE +L G V+D Sbjct: 384 GGMTRMPRVVETVKSIFK-REPAKSVNPDEAVAIGAAIQGGVLSG----HVKDLVLLDVT 438 Query: 398 XXXXGIETAGGVMTTLIKRN 457 GIET GGV T LI RN Sbjct: 439 PLSLGIETLGGVFTRLINRN 458 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 66.5 bits (155), Expect = 4e-12 Identities = 31/79 (39%), Positives = 52/79 (65%) Frame = +3 Query: 12 RAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDK 191 R K LS++++A I+ L +GIDF ITR+ E L D+ + +EP+ K+L+ A + Sbjct: 303 RVKHILSANSEAIASIEELADGIDFRLKITRSVLESLCKDMEDAAVEPINKALKKANLTF 362 Query: 192 AQIHDIVLVGGSTRIPKVQ 248 ++I+ I+L GG++RIP +Q Sbjct: 363 SEINSIILFGGASRIPFIQ 381 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 64.5 bits (150), Expect = 1e-11 Identities = 31/92 (33%), Positives = 53/92 (57%) Frame = +3 Query: 6 CERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKM 185 CE KR LS+ T AS +DSL +GIDF++SI R R++ + + V +++ A M Sbjct: 291 CEITKRVLSNGTTASAAVDSLADGIDFHSSINRLRYDLAASATLNRMADLVTEAVEKANM 350 Query: 186 DKAQIHDIVLVGGSTRIPKVQKLCKISLMERS 281 + I +++L GG++ PK+ L + E++ Sbjct: 351 EPFDISEVILAGGASNTPKLTSLMESIFPEQT 382 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 60.5 bits (140), Expect = 2e-10 Identities = 30/84 (35%), Positives = 49/84 (58%) Frame = +3 Query: 3 ACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAK 182 A ER K+ LS++ A + ++ + ID + I R+ FEEL L P+EK+L A Sbjct: 272 AVERLKKVLSANANAPLNVEMIMNDIDASSFIKRSDFEELIKPLLERLTPPIEKALELAG 331 Query: 183 MDKAQIHDIVLVGGSTRIPKVQKL 254 + K ++ I +VGG TR+P V+++ Sbjct: 332 IKKEDLYSIEMVGGCTRVPIVKEV 355 >SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 932 Score = 26.2 bits (55), Expect = 4.7 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = -3 Query: 204 RGFEPYPSWHHGETSPLAP 148 + +P P+ HG+TSPL+P Sbjct: 420 KAIKPMPTSIHGQTSPLSP 438 >SPAC3G6.05 |||Mvp17/PMP22 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 206 Score = 26.2 bits (55), Expect = 4.7 Identities = 13/28 (46%), Positives = 17/28 (60%) Frame = -2 Query: 304 VRVNRFVELLSIKEILQSFCTLGIRVEP 221 VR+N E+ SIK +LQ F T G + P Sbjct: 55 VRLNTHPEIPSIKRVLQ-FVTFGFAISP 81 >SPAC2C4.15c |ubx2|ucp13|UBX domain protein Ubx2|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 25.8 bits (54), Expect = 6.3 Identities = 16/41 (39%), Positives = 21/41 (51%) Frame = -1 Query: 656 PLASMSKVTSI*GTPRGAGGIPVSSNLPSKLLSLVIARSPS 534 PLA MS TSI G G GG P + + + + +SPS Sbjct: 81 PLADMSAGTSIMGNNFGFGGFPRMNRRQRRRMG-IFDQSPS 120 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 25.8 bits (54), Expect = 6.3 Identities = 17/53 (32%), Positives = 27/53 (50%) Frame = +3 Query: 63 SLFEGIDFYTSITRARFEELNADLFRSTMEPVEKSLRDAKMDKAQIHDIVLVG 221 SL G+D+YT I E +A +S+ E +K D + D++ D + VG Sbjct: 331 SLARGLDYYTGIIYEAVTEASAPKIKSSAE--KKKSADPEADRSN-DDSIGVG 380 >SPAC9.10 |||amino acid permease, unknown 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.4 bits (53), Expect = 8.3 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 4/34 (11%) Frame = +1 Query: 130 ICSGLPWSQ----WRSLSVMPRWIRLKSTILYWW 219 ICS LP + W + S PR+ R S ++ WW Sbjct: 133 ICSALPAAGSIYLWAAESAGPRFGRFVSFLVAWW 166 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,822,816 Number of Sequences: 5004 Number of extensions: 56497 Number of successful extensions: 192 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 174 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 191 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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