BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0529X.Seq (351 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g20780.1 68417.m03017 calcium-binding protein, putative simil... 29 0.87 At4g30190.1 68417.m04292 ATPase 2, plasma membrane-type, putativ... 29 1.2 At2g18960.1 68415.m02213 ATPase 1, plasma membrane-type, putativ... 27 2.7 At1g06140.1 68414.m00645 pentatricopeptide (PPR) repeat-containi... 27 2.7 At1g67930.1 68414.m07757 Golgi transport complex protein-related... 27 4.6 At2g27900.1 68415.m03382 expressed protein 26 6.1 At1g79190.1 68414.m09233 expressed protein 26 6.1 At1g32330.1 68414.m03983 heat shock transcription factor family ... 26 6.1 At2g39330.1 68415.m04828 jacalin lectin family protein similar t... 26 8.1 At1g78130.1 68414.m09105 transporter-related low similarity to s... 26 8.1 At1g56290.1 68414.m06471 CwfJ-like family protein contains Pfam ... 26 8.1 >At4g20780.1 68417.m03017 calcium-binding protein, putative similar to SP|Q09011 Calcium-binding protein CAST {Solanum tuberosum}; contains INTERPRO:IPR002048 calcium-binding EF-hand domain Length = 191 Score = 29.1 bits (62), Expect = 0.87 Identities = 14/39 (35%), Positives = 22/39 (56%) Frame = -3 Query: 211 ETAGAAERRLDLFHGIQVFNVNRIGFLFFRILQNILESV 95 + + AAE DL +VF+ N GF+ R LQ +L+ + Sbjct: 110 DPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKL 148 >At4g30190.1 68417.m04292 ATPase 2, plasma membrane-type, putative / proton pump 2, putative / proton-exporting ATPase, putative strong similarity to SP|P19456 ATPase 2, plasma membrane-type (EC 3.6.3.6) (Proton pump 2) {Arabidopsis thaliana}; contains InterPro accession IPR001757: ATPase, E1-E2 type; contains Pfam profile PF00690: Cation transporter/ATPase, N-terminus Length = 948 Score = 28.7 bits (61), Expect = 1.2 Identities = 14/36 (38%), Positives = 19/36 (52%) Frame = +1 Query: 31 KEHGSGYPESHILLVQRNYLGLQIQECFEVFERKGS 138 K++G E+ L QR GLQ +E +F KGS Sbjct: 864 KDYGKEEREAQWALAQRTLHGLQPKEAVNIFPEKGS 899 >At2g18960.1 68415.m02213 ATPase 1, plasma membrane-type, putative / proton pump 1, putative / proton-exporting ATPase, putative strong similarity to SP|P20649 ATPase 1, plasma membrane-type (EC 3.6.3.6) (Proton pump 1) {Arabidopsis thaliana}; contains InterPro accession IPR001757: ATPase, E1-E2 type; contains Pfam profile PF00690: Cation transporter/ATPase, N-terminus Length = 949 Score = 27.5 bits (58), Expect = 2.7 Identities = 14/36 (38%), Positives = 19/36 (52%) Frame = +1 Query: 31 KEHGSGYPESHILLVQRNYLGLQIQECFEVFERKGS 138 K++G G E+ QR GLQ +E +F KGS Sbjct: 864 KDYGIGEREAQWAQAQRTLHGLQPKEDVNIFPEKGS 899 >At1g06140.1 68414.m00645 pentatricopeptide (PPR) repeat-containing protein contains INTERPRO:IPR002885 PPR repeats Length = 558 Score = 27.5 bits (58), Expect = 2.7 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = +2 Query: 83 IIWGYRFKNVLKYSKEKEAYAI 148 ++WG K LKYSK+ E + + Sbjct: 176 VLWGVLMKGYLKYSKDPEVFRL 197 >At1g67930.1 68414.m07757 Golgi transport complex protein-related similar to golgi transport complex protein (GTC90) GB:5453670 [Homo sapiens] (stimulates in vitro Golgi transport J. Biol. Chem. 273 (45), 29565-29576 (1998)) Length = 832 Score = 26.6 bits (56), Expect = 4.6 Identities = 15/35 (42%), Positives = 21/35 (60%) Frame = +1 Query: 241 KHPNRLTQQMSISPTDLSLSQEDSSTISKLGDSLQ 345 +HP L Q S+S D+SLS SS +S L S++ Sbjct: 104 RHPELLAQLSSLSHADVSLSSLRSS-VSSLQSSIR 137 >At2g27900.1 68415.m03382 expressed protein Length = 1124 Score = 26.2 bits (55), Expect = 6.1 Identities = 11/41 (26%), Positives = 23/41 (56%) Frame = +2 Query: 95 YRFKNVLKYSKEKEAYAIDIENLDTVEQVETPLCSACRLKD 217 Y + ++ ++ EKEA + +L T + V++ S+C +D Sbjct: 413 YSYHEIMSFTPEKEAEILVSPSLATTQMVDSVTGSSCDPQD 453 >At1g79190.1 68414.m09233 expressed protein Length = 1274 Score = 26.2 bits (55), Expect = 6.1 Identities = 14/45 (31%), Positives = 25/45 (55%) Frame = +2 Query: 104 KNVLKYSKEKEAYAIDIENLDTVEQVETPLCSACRLKDFEDNMKD 238 + V+ +K +EAY + E +T+E+V S +LKD+ + D Sbjct: 981 EGVVALAKVEEAYRAETETKETIEEV-IEFASFYQLKDYMNASDD 1024 >At1g32330.1 68414.m03983 heat shock transcription factor family protein contains Pfam profile: PF00447 HSF-type DNA-binding domain Length = 485 Score = 26.2 bits (55), Expect = 6.1 Identities = 12/27 (44%), Positives = 19/27 (70%), Gaps = 1/27 (3%) Frame = +1 Query: 232 ERLKHPNRLTQQMS-ISPTDLSLSQED 309 ++ KH + LTQQM +SP L LS+++ Sbjct: 458 DKTKHMDNLTQQMGLLSPETLDLSRQN 484 >At2g39330.1 68415.m04828 jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI:2997767, myrosinase binding protein [Brassica napus] GI:1711296; contains Pfam profile PF01419 jacalin-like lectin domain Length = 459 Score = 25.8 bits (54), Expect = 8.1 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +1 Query: 31 KEHGSGYPESHILLVQRNYLGLQI 102 KE YP+ HI+ V+ NY G+ + Sbjct: 66 KEFVLQYPDEHIIAVEGNYRGVAL 89 >At1g78130.1 68414.m09105 transporter-related low similarity to spinster type III [Drosophila melanogaster] GI:12003974; contains Pfam profile: PF00083 major facilitator superfamily protein Length = 490 Score = 25.8 bits (54), Expect = 8.1 Identities = 15/38 (39%), Positives = 19/38 (50%) Frame = -3 Query: 301 GLKIGLLAKWTFVGSSGWGV*VFHVIFEILETAGAAER 188 GL L+A TF+G GW V FH++ I G R Sbjct: 147 GLCSVLIAPLTFMGIPGWRV-AFHIVGVISVIVGVLVR 183 >At1g56290.1 68414.m06471 CwfJ-like family protein contains Pfam profiles PF04677: Protein similar to CwfJ N terminus 1, PF04676: Protein similar to CwfJ N terminus 2 Length = 692 Score = 25.8 bits (54), Expect = 8.1 Identities = 9/14 (64%), Positives = 12/14 (85%) Frame = +1 Query: 16 LNRFFKEHGSGYPE 57 LN + KE+G+GYPE Sbjct: 117 LNPYLKENGTGYPE 130 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,330,915 Number of Sequences: 28952 Number of extensions: 162334 Number of successful extensions: 436 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 433 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 436 length of database: 12,070,560 effective HSP length: 72 effective length of database: 9,986,016 effective search space used: 439384704 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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