Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0526.Seq
(670 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C) 147 5e-36
At3g53870.1 68416.m05951 40S ribosomal protein S3 (RPS3B) riboso... 146 2e-35
At2g31610.1 68415.m03862 40S ribosomal protein S3 (RPS3A) 146 2e-35
At3g06190.2 68416.m00712 speckle-type POZ protein-related simila... 32 0.40
At3g06190.1 68416.m00711 speckle-type POZ protein-related simila... 32 0.40
At2g25150.1 68415.m03008 transferase family protein similar to 1... 30 1.6
At1g33811.1 68414.m04180 GDSL-motif lipase/hydrolase family prot... 29 3.7
At1g09830.1 68414.m01105 phosphoribosylamine--glycine ligase (PU... 28 6.5
At5g19000.1 68418.m02257 speckle-type POZ protein-related contai... 27 8.5
At3g09850.1 68416.m01175 D111/G-patch domain-containing protein ... 27 8.5
>At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C)
Length = 248
Score = 147 bits (357), Expect = 5e-36
Identities = 72/85 (84%), Positives = 78/85 (91%)
Frame = +3
Query: 3 SKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGRR 182
SKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGRR
Sbjct: 6 SKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRR 65
Query: 183 IRELTSVVQKRFNIPEQSVELYAER 257
IRELTS+VQKRF P+ SVELYAE+
Sbjct: 66 IRELTSLVQKRFKFPQDSVELYAEK 90
Score = 145 bits (352), Expect = 2e-35
Identities = 66/85 (77%), Positives = 74/85 (87%)
Frame = +2
Query: 254 KVATRGLCAIAQAESLRYKLIGGLAVRRACYGVLRFIMESGARGCEVVVSGKLRGQRAKS 433
KVA RGLCAIAQAESLRYKL+GGLAVRRACYGVLRF+MESGA+GCEV+VSGKLR RAKS
Sbjct: 90 KVANRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAARAKS 149
Query: 434 MKFVDGLMIHSGDPCNDYVNTATRH 508
MKF DG M+ SG P +Y++ A RH
Sbjct: 150 MKFKDGYMVSSGQPTKEYIDAAVRH 174
Score = 65.3 bits (152), Expect = 3e-11
Identities = 28/52 (53%), Positives = 37/52 (71%)
Frame = +1
Query: 508 LLLRQGVLGIKVKIMLPWDQQGKNGPKKPQPDHILVTEPKDEPVPLEPTSEV 663
+LLRQGVLG+KVKIML WD +GK GP P PD +++ PK++ V + P V
Sbjct: 175 VLLRQGVLGLKVKIMLDWDPKGKQGPMTPLPDVVIIHTPKEDDVYIAPAQVV 226
>At3g53870.1 68416.m05951 40S ribosomal protein S3 (RPS3B) ribosomal
protein S3a - Xenopus laevis, PIR:R3XL3A
Length = 249
Score = 146 bits (353), Expect = 2e-35
Identities = 72/85 (84%), Positives = 77/85 (90%)
Frame = +3
Query: 3 SKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGRR 182
SKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGRR
Sbjct: 6 SKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRR 65
Query: 183 IRELTSVVQKRFNIPEQSVELYAER 257
IRELTS+VQKRF P SVELYAE+
Sbjct: 66 IRELTSLVQKRFKFPVDSVELYAEK 90
Score = 143 bits (347), Expect = 8e-35
Identities = 65/85 (76%), Positives = 74/85 (87%)
Frame = +2
Query: 254 KVATRGLCAIAQAESLRYKLIGGLAVRRACYGVLRFIMESGARGCEVVVSGKLRGQRAKS 433
KV RGLCAIAQAESLRYKL+GGLAVRRACYGVLRF+MESGA+GCEV+VSGKLR RAKS
Sbjct: 90 KVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAARAKS 149
Query: 434 MKFVDGLMIHSGDPCNDYVNTATRH 508
MKF DG M+ SG P +Y+++A RH
Sbjct: 150 MKFKDGYMVSSGQPTKEYIDSAVRH 174
Score = 63.3 bits (147), Expect = 1e-10
Identities = 27/48 (56%), Positives = 35/48 (72%)
Frame = +1
Query: 508 LLLRQGVLGIKVKIMLPWDQQGKNGPKKPQPDHILVTEPKDEPVPLEP 651
+LLRQGVLGIKVK+ML WD +G +GPK P PD +++ PK+E P
Sbjct: 175 VLLRQGVLGIKVKVMLDWDPKGISGPKTPLPDVVIIHSPKEEEAIYAP 222
>At2g31610.1 68415.m03862 40S ribosomal protein S3 (RPS3A)
Length = 250
Score = 146 bits (353), Expect = 2e-35
Identities = 72/85 (84%), Positives = 77/85 (90%)
Frame = +3
Query: 3 SKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGRR 182
SKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGRR
Sbjct: 6 SKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRR 65
Query: 183 IRELTSVVQKRFNIPEQSVELYAER 257
IRELTS+VQKRF P SVELYAE+
Sbjct: 66 IRELTSLVQKRFKFPVDSVELYAEK 90
Score = 143 bits (346), Expect = 1e-34
Identities = 65/85 (76%), Positives = 73/85 (85%)
Frame = +2
Query: 254 KVATRGLCAIAQAESLRYKLIGGLAVRRACYGVLRFIMESGARGCEVVVSGKLRGQRAKS 433
KV RGLCAIAQAESLRYKL+GGLAVRRACYGVLRF+MESGA+GCEV+VSGKLR RAKS
Sbjct: 90 KVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAARAKS 149
Query: 434 MKFVDGLMIHSGDPCNDYVNTATRH 508
MKF DG M+ SG P +Y++ A RH
Sbjct: 150 MKFKDGYMVSSGQPTKEYIDAAVRH 174
Score = 66.9 bits (156), Expect = 1e-11
Identities = 30/48 (62%), Positives = 36/48 (75%)
Frame = +1
Query: 508 LLLRQGVLGIKVKIMLPWDQQGKNGPKKPQPDHILVTEPKDEPVPLEP 651
+LLRQGVLGIKVKIML WD GK+GPK P PD +++ PKD+ V P
Sbjct: 175 VLLRQGVLGIKVKIMLDWDPTGKSGPKTPLPDVVIIHAPKDDVVYSAP 222
>At3g06190.2 68416.m00712 speckle-type POZ protein-related similar
to SPOP (novel nuclear speckle-type protein)
(SP:O43791) [Homo sapiens]; contains Pfam PF00651 :
BTB/POZ domain; contains Pfam PF00917: MATH domain
Length = 295
Score = 31.9 bits (69), Expect = 0.40
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3
Query: 197 SQLTDSASFLSEHTLCPGGHNNDLRADGSD 108
S+L + + LSEH+L GH +L ADG D
Sbjct: 255 SELLEYVARLSEHSLTSSGHRKELFADGCD 284
>At3g06190.1 68416.m00711 speckle-type POZ protein-related similar
to SPOP (novel nuclear speckle-type protein)
(SP:O43791) [Homo sapiens]; contains Pfam PF00651 :
BTB/POZ domain; contains Pfam PF00917: MATH domain
Length = 406
Score = 31.9 bits (69), Expect = 0.40
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3
Query: 197 SQLTDSASFLSEHTLCPGGHNNDLRADGSD 108
S+L + + LSEH+L GH +L ADG D
Sbjct: 366 SELLEYVARLSEHSLTSSGHRKELFADGCD 395
>At2g25150.1 68415.m03008 transferase family protein similar to
10-deacetylbaccatin III-10-O-acetyl transferase
[gi:6746554], 2-debenzoyl-7,13-diacetylbaccatin
III-2-O-benzoyl transferase [gi:11559716] from Taxus
cuspidata; contains Pfam transferase family domain
PF00248; contains EST gb:R65039
Length = 461
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -3
Query: 128 LRADGSDPHFHAGVAVLGQLPSEELIEFRLE 36
L+ SDP GV V+ +LP + ++EF+ E
Sbjct: 418 LKPSKSDPSMEGGVKVIMKLPRDAMVEFKRE 448
>At1g33811.1 68414.m04180 GDSL-motif lipase/hydrolase family protein
similar to family II lipases EXL3 GI:15054386, EXL1
GI:15054382, EXL2 GI:15054384 from [Arabidopsis
thaliana]; contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase
Length = 370
Score = 28.7 bits (61), Expect = 3.7
Identities = 20/64 (31%), Positives = 29/64 (45%)
Frame = -1
Query: 427 GTLTTQLARYNNFTTTGTRFHDETENTIASTTYSETSDKLVS*RFGLGDSAKTTSSHLSA 248
G + QLARYNN + R +++ N I ++ KLV R G +L +
Sbjct: 228 GCIPYQLARYNNRNNSTGRCNEKINNAI--VVFNTQVKKLVD-RLNKGQLKGAKFVYLDS 284
Query: 247 YNST 236
Y ST
Sbjct: 285 YKST 288
>At1g09830.1 68414.m01105 phosphoribosylamine--glycine ligase (PUR2)
Identical to phosphoribosylamine--glycine ligase,
chloroplast [precursor] SP:P52420 from [Arabidopsis
thaliana]
Length = 532
Score = 27.9 bits (59), Expect = 6.5
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +2
Query: 374 GARGCEVVVSGKLRGQRAKSMKFVDG 451
G+ GC+VVV L G+ A VDG
Sbjct: 273 GSAGCQVVVEEFLEGEEASFFALVDG 298
>At5g19000.1 68418.m02257 speckle-type POZ protein-related contains
Pfam PF00651 : BTB/POZ domain; contains Pfam PF00917:
MATH domain; similar to Speckle-type POZ protein
(SP:O43791) [Homo sapiens]
Length = 442
Score = 27.5 bits (58), Expect = 8.5
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = -3
Query: 197 SQLTDSASFLSEHTLCPGGHNNDLRADGSD 108
++L + LSEH++ GH ++ ADG D
Sbjct: 402 TELLQYVARLSEHSVIVSGHRKEIFADGCD 431
>At3g09850.1 68416.m01175 D111/G-patch domain-containing protein
contains Pfam profile PF01585: G-patch domain
Length = 781
Score = 27.5 bits (58), Expect = 8.5
Identities = 15/59 (25%), Positives = 29/59 (49%)
Frame = +3
Query: 6 KKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGRR 182
K RK G + KA + E+ ++ GYSG P+ + ++ +S+ G+K ++
Sbjct: 349 KTRKLTGIRLQKASM-EYGKKKTTRSGYSGHGKSTNPLAMDDLMFVKDPRSLSGKKNKK 406
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,335,683
Number of Sequences: 28952
Number of extensions: 332309
Number of successful extensions: 909
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 864
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 909
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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