BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0509.Seq (738 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 56 5e-09 SPAC13D6.05 |alp11|SPAC4G9.01|tubulin specific chaperone cofacto... 54 2e-08 SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.52 SPBP19A11.04c |mor2|cps12|morphogenesis protein Mor2|Schizosacch... 29 0.52 SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch... 29 0.91 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 28 1.2 SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|... 28 1.2 SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1 |Schizosacch... 28 1.2 SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 27 2.1 SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 27 2.8 SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 27 3.7 SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 26 4.9 SPCC1183.04c |pet127||mitochondrial membrane protein Pet127|Schi... 26 6.4 SPCC1494.04c |tyr1||prephenate dehydrogenase [NADP+] |Schizosacc... 25 8.5 SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 25 8.5 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 56.0 bits (129), Expect = 5e-09 Identities = 26/61 (42%), Positives = 36/61 (59%) Frame = +3 Query: 264 IGERVWVGGTKPGQIAYIGETQFAPGEWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFS 443 +G+ V + G + G + + G T F G W G+ L GKNDGSV G RYF C + +G+F Sbjct: 6 VGDEVLIRG-ELGIVRFAGSTDFESGIWLGVELLNGKGKNDGSVKGKRYFSCEKGKGIFV 64 Query: 444 R 446 R Sbjct: 65 R 65 >SPAC13D6.05 |alp11|SPAC4G9.01|tubulin specific chaperone cofactor B|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 54.4 bits (125), Expect = 2e-08 Identities = 26/63 (41%), Positives = 33/63 (52%), Gaps = 2/63 (3%) Frame = +3 Query: 264 IGERVWVGGTKPGQIAYIGETQFAPGE--WAGIVLDEPIGKNDGSVAGVRYFQCPEKRGV 437 + R G + G I YIG + W G+ DEP+GKNDG+V+G RYF K G Sbjct: 154 LNSRCCAAGERYGTIRYIGLVPEINNDNLWVGVEFDEPVGKNDGTVSGKRYFNAKNKHGS 213 Query: 438 FSR 446 F R Sbjct: 214 FLR 216 >SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1379 Score = 29.5 bits (63), Expect = 0.52 Identities = 15/60 (25%), Positives = 34/60 (56%) Frame = +1 Query: 130 MPVETKISFSDGSSTDTLRKLSDDLSRKHLSDHSVILTEDTEASLLVNVFG*AVRSLDKS 309 +P++ K+S S SST +++ L + ++KH+ HS I D ++ ++ ++ +K+ Sbjct: 855 LPMKKKLSHSSASSTRSIKSLLTNANQKHI--HSSINETDDIPTMAAHIVATGQKNKEKA 912 >SPBP19A11.04c |mor2|cps12|morphogenesis protein Mor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2196 Score = 29.5 bits (63), Expect = 0.52 Identities = 14/50 (28%), Positives = 23/50 (46%) Frame = +1 Query: 370 QLVKTMDQWQAFVIFNVQRNEVCFLD*LGSLEFPSLYTHRTMHRPCRCGE 519 Q V + Q F++F + N C+LD + +LY + PC G+ Sbjct: 500 QFVHNLSQKLLFLVFQLSTNTNCYLDSKNATSLLALYINALKVFPCLMGK 549 >SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1092 Score = 28.7 bits (61), Expect = 0.91 Identities = 13/40 (32%), Positives = 23/40 (57%) Frame = -1 Query: 651 VRISPLVVVEIEAFILLLTILRILPFGERVRLGRAAPEGG 532 +++ PL+ E++ F L+TI R+L G + L + E G Sbjct: 47 IKVQPLLANEVQTFKALITIHRVLQEGHKSALVDSQSEKG 86 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 28.3 bits (60), Expect = 1.2 Identities = 26/95 (27%), Positives = 41/95 (43%), Gaps = 3/95 (3%) Frame = +2 Query: 413 SMSRETRCVFSIDSAH*SSLRYTHTARCIARVDAGSVFERPPSGAAR-PKRTLSPNGSIR 589 S S T +I S+ SS ++ T + + S P S ++ + SP+ S Sbjct: 296 SSSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSSSPSSSSF 355 Query: 590 SIV--SSKMNASISTTTNGDIRTGDRVIVSSSRGS 688 S SSK ++S S+T + T + SSS S Sbjct: 356 SSTTSSSKSSSSFSSTVSSSSSTSSSTLTSSSSSS 390 >SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 194 Score = 28.3 bits (60), Expect = 1.2 Identities = 12/15 (80%), Positives = 12/15 (80%) Frame = -2 Query: 545 RRKEVVRTRSPHRHG 501 RRKE RTRS HRHG Sbjct: 136 RRKEKERTRSNHRHG 150 >SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 469 Score = 28.3 bits (60), Expect = 1.2 Identities = 20/63 (31%), Positives = 36/63 (57%), Gaps = 3/63 (4%) Frame = +2 Query: 356 STRRANW*KRWISGRRSLFSMS---RETRCVFSIDSAH*SSLRYTHTARCIARVDAGSVF 526 S R+N + I GRR++FS+S +E R +F++ SA + + ++ + + DA S Sbjct: 194 SLERSNLKRLHIVGRRNIFSVSFTIKELRELFALSSAVFFAPSFNYSTKWMNETDA-SGL 252 Query: 527 ERP 535 +RP Sbjct: 253 DRP 255 >SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 27.5 bits (58), Expect = 2.1 Identities = 15/35 (42%), Positives = 23/35 (65%) Frame = +1 Query: 118 ENHKMPVETKISFSDGSSTDTLRKLSDDLSRKHLS 222 EN +PV +KI+ + DTL +++D LSR H+S Sbjct: 118 ENENLPV-SKIA-EVTNELDTLVRINDQLSRNHIS 150 >SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 27.1 bits (57), Expect = 2.8 Identities = 9/24 (37%), Positives = 18/24 (75%) Frame = +2 Query: 656 DRVIVSSSRGSKSWVILRFRKVLQ 727 D+++++S+ G SW+++R VLQ Sbjct: 469 DKLLINSALGFDSWLVMRHGSVLQ 492 >SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1072 Score = 26.6 bits (56), Expect = 3.7 Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 2/56 (3%) Frame = +1 Query: 112 FFENHKMPV--ETKISFSDGSSTDTLRKLSDDLSRKHLSDHSVILTEDTEASLLVN 273 F +N + + E + D STD L+K D + HL + +VI ++T+ S+ VN Sbjct: 599 FEQNQTLDILSENIVEIQD-KSTDPLQKSLDSSLKSHLPEATVI--QNTDGSVTVN 651 >SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2111 Score = 26.2 bits (55), Expect = 4.9 Identities = 10/37 (27%), Positives = 21/37 (56%) Frame = +2 Query: 587 RSIVSSKMNASISTTTNGDIRTGDRVIVSSSRGSKSW 697 +++V + + + T+G IRTG V ++ G++ W Sbjct: 1144 QTLVLNDLRGRVVIQTDGQIRTGRDVAIACLLGAEEW 1180 >SPCC1183.04c |pet127||mitochondrial membrane protein Pet127|Schizosaccharomyces pombe|chr 3|||Manual Length = 524 Score = 25.8 bits (54), Expect = 6.4 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 2/47 (4%) Frame = -2 Query: 335 SKLRFSNISDLSRLRTAYPN--TFTNNEASVSSVNITLWSDRCFLDR 201 SK +F N SDLS+ PN FT S SSV+I+ + +D+ Sbjct: 146 SKWKFPNFSDLSKGFYISPNERNFTRLCRSASSVHISYQNGLYCIDK 192 >SPCC1494.04c |tyr1||prephenate dehydrogenase [NADP+] |Schizosaccharomyces pombe|chr 3|||Manual Length = 431 Score = 25.4 bits (53), Expect = 8.5 Identities = 15/62 (24%), Positives = 32/62 (51%), Gaps = 1/62 (1%) Frame = +2 Query: 278 LGRRYEAWTNRLYWRNAVCSRRVGWDSTRRANW*KRWISGRRSLFSMSRETRCV-FSIDS 454 +GR Y + ++ WR VC R ++S +A + + + F +SR + + +S+++ Sbjct: 15 MGRLYAEYISKAGWRVNVCDRPENYESI-QATYGNGGYTVLKDGFQVSRTSDYILYSVEA 73 Query: 455 AH 460 H Sbjct: 74 EH 75 >SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosaccharomyces pombe|chr 1|||Manual Length = 1168 Score = 25.4 bits (53), Expect = 8.5 Identities = 14/45 (31%), Positives = 24/45 (53%), Gaps = 1/45 (2%) Frame = -2 Query: 635 WSLWKLKHSSYC*QYC-EYFHLARGSAWDEPRRKEVVRTRSPHRH 504 ++ WK+ S +C E++ ARG E RK+++R +RH Sbjct: 995 YTTWKMNRCSD--NWCYEHYIQARGMRRAEDVRKQLIRLMDRYRH 1037 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,219,523 Number of Sequences: 5004 Number of extensions: 68966 Number of successful extensions: 233 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 220 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 233 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 349251756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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