Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0509.Seq
(738 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10220.1 68416.m01223 tubulin folding cofactor B identical to... 62 3e-10
At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferlin... 42 4e-04
At3g11960.1 68416.m01475 cleavage and polyadenylation specificit... 31 0.80
At3g52640.2 68416.m05800 nicastrin-related contains weak similar... 29 3.2
At3g52640.1 68416.m05799 nicastrin-related contains weak similar... 29 3.2
At1g57600.1 68414.m06536 membrane bound O-acyl transferase (MBOA... 29 4.3
At4g28950.1 68417.m04136 Rac-like GTP-binding protein (ARAC7) id... 28 5.6
At5g13000.1 68418.m01490 glycosyl transferase family 48 protein ... 28 7.4
At4g27910.1 68417.m04006 PHD finger protein-related / SET domain... 28 7.4
At3g11960.2 68416.m01476 cleavage and polyadenylation specificit... 28 7.4
At1g62320.1 68414.m07032 early-responsive to dehydration protein... 28 7.4
At1g33170.1 68414.m04096 dehydration-responsive family protein s... 28 7.4
At5g64270.1 68418.m08074 splicing factor, putative similar to sp... 27 9.8
At5g43040.1 68418.m05254 DC1 domain-containing protein contains ... 27 9.8
At5g43030.1 68418.m05250 DC1 domain-containing protein contains ... 27 9.8
At4g21370.1 68417.m03088 S-locus protein kinase, putative simila... 27 9.8
At3g13300.2 68416.m01675 transducin family protein / WD-40 repea... 27 9.8
At3g13300.1 68416.m01674 transducin family protein / WD-40 repea... 27 9.8
At3g09400.1 68416.m01116 protein phosphatase 2C family protein /... 27 9.8
At2g33060.1 68415.m04054 leucine-rich repeat family protein cont... 27 9.8
At2g31960.1 68415.m03905 glycosyl transferase family 48 protein ... 27 9.8
At1g54100.2 68414.m06167 aldehyde dehydrogenase, putative / anti... 27 9.8
At1g54100.1 68414.m06166 aldehyde dehydrogenase, putative / anti... 27 9.8
At1g27370.2 68414.m03335 squamosa promoter-binding protein-like ... 27 9.8
At1g27370.1 68414.m03334 squamosa promoter-binding protein-like ... 27 9.8
At1g05570.1 68414.m00575 callose synthase 1 (CALS1) / 1,3-beta-g... 27 9.8
>At3g10220.1 68416.m01223 tubulin folding cofactor B identical to
tubulin folding cofactor B GI:20514259 from [Arabidopsis
thaliana]; identical to cDNA tubulin folding cofactor B
GI:20514258
Length = 243
Score = 62.5 bits (145), Expect = 3e-10
Identities = 27/59 (45%), Positives = 37/59 (62%), Gaps = 2/59 (3%)
Frame = +3
Query: 264 IGERVWVG-GTKPGQIAYIGETQ-FAPGEWAGIVLDEPIGKNDGSVAGVRYFQCPEKRG 434
+G+R V G K G + Y+G + PG W GI DEP+GK+DG V G R+F+CP +G
Sbjct: 161 VGDRCQVEPGEKRGMVKYVGRAESLGPGYWVGIQYDEPLGKHDGMVKGTRFFECPRLQG 219
>At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferling
(PFI) almost identical to tubulin folding cofactor E
(Pfifferling; PFI) GI:20514267 from [Arabidopsis
thaliana]; identical to cDNA tubulin folding cofactor E,
GI:20514266
Length = 531
Score = 41.9 bits (94), Expect = 4e-04
Identities = 23/56 (41%), Positives = 33/56 (58%), Gaps = 3/56 (5%)
Frame = +3
Query: 255 SFIIGERVWV--GGTKPGQIAYIGETQFAPGEWAGIVLDEP-IGKNDGSVAGVRYF 413
SFIIG+RV + G + Y+G+ + G W G+ D+ GK++GSV GV YF
Sbjct: 9 SFIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYF 64
>At3g11960.1 68416.m01475 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1379
Score = 31.1 bits (67), Expect = 0.80
Identities = 13/43 (30%), Positives = 26/43 (60%)
Frame = -2
Query: 329 LRFSNISDLSRLRTAYPNTFTNNEASVSSVNITLWSDRCFLDR 201
L S++ D+ R +YP+ N +S+ +++ T+WS CF+ +
Sbjct: 184 LSTSSMGDIIHQRISYPSEDGGNGSSIQAISGTIWS-MCFISK 225
>At3g52640.2 68416.m05800 nicastrin-related contains weak similarity
to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
sapiens]
Length = 705
Score = 29.1 bits (62), Expect = 3.2
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +3
Query: 312 YIGETQFAPGEWAGIVLDEPIGK 380
YI T PG +AG++L EP K
Sbjct: 535 YISPTNTCPGNYAGVILGEPSSK 557
>At3g52640.1 68416.m05799 nicastrin-related contains weak similarity
to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
sapiens]
Length = 676
Score = 29.1 bits (62), Expect = 3.2
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +3
Query: 312 YIGETQFAPGEWAGIVLDEPIGK 380
YI T PG +AG++L EP K
Sbjct: 506 YISPTNTCPGNYAGVILGEPSSK 528
>At1g57600.1 68414.m06536 membrane bound O-acyl transferase (MBOAT)
family protein low similarity to skinny hedgehog
[Drosophila melanogaster] GI:15420842; contains Pfam
profile PF03062: MBOAT family
Length = 533
Score = 28.7 bits (61), Expect = 4.3
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -2
Query: 380 FTNWLVEYYPSPLSGSKLRFSNI 312
F WL+ Y PL GS+ +F N+
Sbjct: 385 FNRWLIRYMYIPLGGSRRKFLNV 407
>At4g28950.1 68417.m04136 Rac-like GTP-binding protein (ARAC7)
identical to rac GTP binding protein Arac7 GI:3702962
from [Arabidopsis thaliana]
Length = 209
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2
Query: 548 PRRKEVVRTRSPHRHGRCIVRCVYSEG 468
PRRKEV R R HR C + + G
Sbjct: 179 PRRKEVPRRRKNHRRSGCSIASIVCGG 205
>At5g13000.1 68418.m01490 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1889
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -3
Query: 532 SFEHAPRIDTGDASCGVCIAKGTLVSRVNRENTPRFSGH*K 410
+FE A R+D + GV K L+ R+ RE+ P G K
Sbjct: 70 AFEKAHRLDPTSSGRGVRQFKTALLQRLEREHDPTLMGRVK 110
>At4g27910.1 68417.m04006 PHD finger protein-related / SET
domain-containing protein (TX4) nearly identical over
285 amino acids to trithorax 4 [Arabidopsis thaliana]
GI:16118405; contains Pfam profiles PF00856: SET domain,
PF00855: PWWP domain; identical to cDNA trithorax 4
(TX4) partial cds GI:16118404
Length = 1027
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +2
Query: 503 RVDAGSVFERPPSGAARPKRTLSPNGSIRSIVSSKMNASI 622
R D+G+V + PP+ +RP + G I+ ++ S+ N S+
Sbjct: 82 RSDSGTVRDSPPAEVSRPPLVRTSRGRIQ-VLPSRFNDSV 120
>At3g11960.2 68416.m01476 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1329
Score = 27.9 bits (59), Expect = 7.4
Identities = 14/44 (31%), Positives = 26/44 (59%)
Frame = -2
Query: 332 KLRFSNISDLSRLRTAYPNTFTNNEASVSSVNITLWSDRCFLDR 201
KL F + S+ R +YP+ N +S+ +++ T+WS CF+ +
Sbjct: 126 KLSFLSFSNEMH-RISYPSEDGGNGSSIQAISGTIWS-MCFISK 167
>At1g62320.1 68414.m07032 early-responsive to dehydration
protein-related / ERD protein-related similar to ERD4
protein (early-responsive to dehydration stress)
[Arabidopsis thaliana] GI:15375406; contains Pfam
profile PF02714: Domain of unknown function DUF221
Length = 769
Score = 27.9 bits (59), Expect = 7.4
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -1
Query: 612 FILLLTILRILPFGERVRLGRAAPEGGRSN 523
F+LL ILRI PF +RV + +G RS+
Sbjct: 21 FLLLFAILRIQPFNDRVYFPKWYLKGVRSS 50
>At1g33170.1 68414.m04096 dehydration-responsive family protein
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 639
Score = 27.9 bits (59), Expect = 7.4
Identities = 19/79 (24%), Positives = 33/79 (41%), Gaps = 2/79 (2%)
Frame = -2
Query: 467 NSSEPSQSRKHTSFLWTLKITNACH*SIVFTNWLVEYYPSPLSG--SKLRFSNISDLSRL 294
NSS K W L ++ C S V +W P+ S S++ S +R
Sbjct: 5 NSSHSLAEAKRKRLTWILCVSGLCILSYVLGSWQTNTVPTSSSEAYSRMGCDETSTTTRA 64
Query: 293 RTAYPNTFTNNEASVSSVN 237
+T T +++ + SS++
Sbjct: 65 QTTQTQTNPSSDDTSSSLS 83
>At5g64270.1 68418.m08074 splicing factor, putative similar to
splicing factor 3B subunit 1 (Spliceosome associated
protein 155) (SAP 155) (SF3b155) (Pre-mRNA splicing
factor SF3b 155 kDa subunit) (146 kDa nuclear protein)
SP:O57683 from [Xenopus laevis]
Length = 1269
Score = 27.5 bits (58), Expect = 9.8
Identities = 26/88 (29%), Positives = 39/88 (44%), Gaps = 8/88 (9%)
Frame = -3
Query: 553 TSRAGRRS-FEHAP---RIDTGDASCGVCIAKGTLVSRVNREN----TPRFSGH*K*RTP 398
T AGRR+ ++ P R+ DA+ G + G S V + TP+ TP
Sbjct: 238 TPSAGRRNRWDETPTPGRVTDSDATPGGGVTPGATPSGVTWDGLATPTPKRQRSRWDETP 297
Query: 397 ATDPSFLPIGSSSTIPAHSPGANCVSPI 314
AT S P+G + A++PG + I
Sbjct: 298 ATMGSATPMGGVTPGAAYTPGVTPIGGI 325
>At5g43040.1 68418.m05254 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 551
Score = 27.5 bits (58), Expect = 9.8
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -3
Query: 523 HAPRIDTGDASCGVC 479
H PR+ +G+ SCGVC
Sbjct: 182 HVPRLGSGNWSCGVC 196
>At5g43030.1 68418.m05250 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 564
Score = 27.5 bits (58), Expect = 9.8
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -3
Query: 523 HAPRIDTGDASCGVC 479
H PR+ +G+ SCGVC
Sbjct: 177 HVPRLGSGNWSCGVC 191
>At4g21370.1 68417.m03088 S-locus protein kinase, putative similar
to SRKa [Arabidopsis lyrata] gi|13620927|dbj|BAB40986;
contains Pfam domains PF00954: S-locus glycoprotein
family, PF00069: Protein kinase domain and PF01453:
Lectin (probable mannose binding)
Length = 844
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -2
Query: 518 SPHRHGRCIVRCVYSEGNSSEPSQSRKHTSFLWTLKIT 405
SP R G C+ R +SS ++ H LW++ T
Sbjct: 803 SPRRPGYCVRRSSLDTDDSSSSTERDNHRRHLWSVLCT 840
>At3g13300.2 68416.m01675 transducin family protein / WD-40 repeat
family protein contains 2 WD-40 repeats (PF00400);
autoantigen locus HUMAUTANT (GI:533202) [Homo sapiens]
and autoantigen locus HSU17474 (GI:596134) [Homo
sapiens]
Length = 1309
Score = 27.5 bits (58), Expect = 9.8
Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 10/82 (12%)
Frame = +3
Query: 372 IGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRV-------PFAIHTPHDASPVSMRGACSN 530
+GK+DG V + Q R V S + ++ P + PHD PVS ++
Sbjct: 317 VGKHDGEVTDLSMCQWMTTRLVSSSVDGTIKIWQDRKAQPLVVLRPHDGHPVSSATFVTS 376
Query: 531 DLLPAR--LVPSGP-SRQMEVF 587
P L+ GP +R+M+++
Sbjct: 377 PERPDHIILITGGPLNREMKIW 398
>At3g13300.1 68416.m01674 transducin family protein / WD-40 repeat
family protein contains 2 WD-40 repeats (PF00400);
autoantigen locus HUMAUTANT (GI:533202) [Homo sapiens]
and autoantigen locus HSU17474 (GI:596134) [Homo
sapiens]
Length = 1344
Score = 27.5 bits (58), Expect = 9.8
Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 10/82 (12%)
Frame = +3
Query: 372 IGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRV-------PFAIHTPHDASPVSMRGACSN 530
+GK+DG V + Q R V S + ++ P + PHD PVS ++
Sbjct: 352 VGKHDGEVTDLSMCQWMTTRLVSSSVDGTIKIWQDRKAQPLVVLRPHDGHPVSSATFVTS 411
Query: 531 DLLPAR--LVPSGP-SRQMEVF 587
P L+ GP +R+M+++
Sbjct: 412 PERPDHIILITGGPLNREMKIW 433
>At3g09400.1 68416.m01116 protein phosphatase 2C family protein /
PP2C family protein similar to protein phosphatase-2c
(GI:3608412) [Mesembryanthemum crystallinum]; contains
Pfam PF00481 : Protein phosphatase 2C domain
Length = 650
Score = 27.5 bits (58), Expect = 9.8
Identities = 21/77 (27%), Positives = 31/77 (40%), Gaps = 1/77 (1%)
Frame = +2
Query: 494 CIAR-VDAGSVFERPPSGAARPKRTLSPNGSIRSIVSSKMNASISTTTNGDIRTGDRVIV 670
C R V GS PP RP + RSI + ++A+ ST + + T I
Sbjct: 40 CYVRPVLTGSKSSFPPEPPLRPDPIPGTTTTFRSISGASVSANTSTALSTSLSTDTSGIA 99
Query: 671 SSSRGSKSWVILRFRKV 721
S+ S + L + V
Sbjct: 100 SAFESSNRFASLPLQPV 116
>At2g33060.1 68415.m04054 leucine-rich repeat family protein
contains leucine rich-repeat domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Hcr2-0B
[Lycopersicon esculentum] gi|3894387|gb|AAC78593
Length = 808
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = -2
Query: 347 PLSGSKLRFSNISDLSRLRTAYPNTFTNNEASVSSVN 237
PL+ KLR ISD + + PN F N EAS +N
Sbjct: 529 PLAFPKLRILEISDNNFTGSLPPNYFVNWEASSLQMN 565
>At2g31960.1 68415.m03905 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase;
contains non-consensus splice aite AC at exon 33
Length = 1959
Score = 27.5 bits (58), Expect = 9.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -3
Query: 532 SFEHAPRIDTGDASCGVCIAKGTLVSRVNRENTPRFSGH*K 410
+FE A R+D + GV K L+ R+ REN +G K
Sbjct: 67 AFEKAHRLDPTSSGRGVRQFKTALLQRLERENETTLAGRQK 107
>At1g54100.2 68414.m06167 aldehyde dehydrogenase, putative /
antiquitin, putative strong similarity to SP|Q41247
Aldehyde dehydrogenase family 7 member A1 (EC 1.2.1.3)
(Antiquitin 1) (Brassica
turgor-responsive/drought-induced gene 26 protein)
(Btg-26) {Brassica napus}; similar to turgor-responsive
protein 26G (aldehyde dehydrogenase family 7 member A1)
[Pisum sativum] SWISS-PROT:P25795
Length = 508
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -1
Query: 630 VVEIEAFILLLTILRILPFGERVRLGRAAPEG 535
VV+ E F +L +L+ FGE V + + P+G
Sbjct: 392 VVKEELFAPVLYVLKFKSFGEAVAINNSVPQG 423
>At1g54100.1 68414.m06166 aldehyde dehydrogenase, putative /
antiquitin, putative strong similarity to SP|Q41247
Aldehyde dehydrogenase family 7 member A1 (EC 1.2.1.3)
(Antiquitin 1) (Brassica
turgor-responsive/drought-induced gene 26 protein)
(Btg-26) {Brassica napus}; similar to turgor-responsive
protein 26G (aldehyde dehydrogenase family 7 member A1)
[Pisum sativum] SWISS-PROT:P25795
Length = 508
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -1
Query: 630 VVEIEAFILLLTILRILPFGERVRLGRAAPEG 535
VV+ E F +L +L+ FGE V + + P+G
Sbjct: 392 VVKEELFAPVLYVLKFKSFGEAVAINNSVPQG 423
>At1g27370.2 68414.m03335 squamosa promoter-binding protein-like 10
(SPL10) identical to squamosa promoter binding
protein-like 10 [Arabidopsis thaliana] GI:5931669;
contains Pfam profile PF03110: SBP domain
Length = 396
Score = 27.5 bits (58), Expect = 9.8
Identities = 21/62 (33%), Positives = 26/62 (41%)
Frame = -2
Query: 605 YC*QYCEYFHLARGSAWDEPRRKEVVRTRSPHRHGRCIVRCVYSEGNSSEPSQSRKHTSF 426
+C Q C FH S +DE +R R R H + R NS R+HTS
Sbjct: 216 FC-QQCSRFHAV--SEFDEKKRS--CRKRLSHHNARRRKPQGVFPLNSERVFDRRQHTSM 270
Query: 425 LW 420
LW
Sbjct: 271 LW 272
>At1g27370.1 68414.m03334 squamosa promoter-binding protein-like 10
(SPL10) identical to squamosa promoter binding
protein-like 10 [Arabidopsis thaliana] GI:5931669;
contains Pfam profile PF03110: SBP domain
Length = 396
Score = 27.5 bits (58), Expect = 9.8
Identities = 21/62 (33%), Positives = 26/62 (41%)
Frame = -2
Query: 605 YC*QYCEYFHLARGSAWDEPRRKEVVRTRSPHRHGRCIVRCVYSEGNSSEPSQSRKHTSF 426
+C Q C FH S +DE +R R R H + R NS R+HTS
Sbjct: 216 FC-QQCSRFHAV--SEFDEKKRS--CRKRLSHHNARRRKPQGVFPLNSERVFDRRQHTSM 270
Query: 425 LW 420
LW
Sbjct: 271 LW 272
>At1g05570.1 68414.m00575 callose synthase 1 (CALS1) /
1,3-beta-glucan synthase 1 nearly identical to callose
synthase 1 catalytic subunit [Arabidopsis thaliana]
GI:13649388
Length = 1922
Score = 27.5 bits (58), Expect = 9.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -3
Query: 532 SFEHAPRIDTGDASCGVCIAKGTLVSRVNRENTPRFSGH*K 410
+FE A R+D + GV K L+ R+ REN +G K
Sbjct: 39 AFEKAHRLDPTSSGRGVRQFKTALLQRLERENETTLAGRQK 79
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,943,067
Number of Sequences: 28952
Number of extensions: 368352
Number of successful extensions: 1185
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1182
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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