Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0508.Seq
(560 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g22790.1 68415.m02703 expressed protein ; expression supporte... 32 0.23
At3g09100.2 68416.m01070 mRNA capping enzyme family protein simi... 29 1.6
At3g09100.1 68416.m01071 mRNA capping enzyme family protein simi... 29 1.6
At1g61080.1 68414.m06877 proline-rich family protein 29 1.6
At4g15270.1 68417.m02339 glucosyltransferase-related contains so... 29 2.1
At5g03730.2 68418.m00334 serine/threonine protein kinase (CTR1) ... 29 2.8
At5g03730.1 68418.m00333 serine/threonine protein kinase (CTR1) ... 29 2.8
At3g50350.1 68416.m05506 expressed protein 28 3.7
At1g09860.1 68414.m01110 purine permease-related low similarity ... 28 3.7
At5g58720.1 68418.m07356 PRLI-interacting factor, putative simil... 28 4.9
At2g40475.1 68415.m04995 expressed protein 28 4.9
At5g58400.1 68418.m07313 peroxidase, putative similar to peroxid... 27 6.5
At3g27210.1 68416.m03402 expressed protein 27 6.5
At1g71300.1 68414.m08228 Vps52/Sac2 family protein similar to SP... 27 6.5
At3g29150.1 68416.m03649 expressed protein 27 8.6
At1g72270.1 68414.m08355 expressed protein 27 8.6
>At2g22790.1 68415.m02703 expressed protein ; expression supported
by MPSS
Length = 325
Score = 32.3 bits (70), Expect = 0.23
Identities = 19/65 (29%), Positives = 32/65 (49%)
Frame = -3
Query: 471 ASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVPDLVMLDL*QMN 292
A G ++ ++ RDW+ A EG+ CLD + R ++ +V D++M+D N
Sbjct: 212 AEGWRFVVARDYTRDWWSALGFEGLTACLDDAFWKLREAGWTED--DVRDMMMMDSVDQN 269
Query: 291 DEIHQ 277
I Q
Sbjct: 270 TCIQQ 274
>At3g09100.2 68416.m01070 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF00782: Dual specificity phosphatase
catalytic domain
Length = 672
Score = 29.5 bits (63), Expect = 1.6
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -2
Query: 235 KYLANKNVKVWIRYVVVPGWSDDDDSAHRLG 143
+Y N+N K + RY + GW D S + +G
Sbjct: 68 QYYQNRNTKAYDRYKIPQGWLDCPPSGNEIG 98
>At3g09100.1 68416.m01071 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF00782: Dual specificity phosphatase
catalytic domain
Length = 471
Score = 29.5 bits (63), Expect = 1.6
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -2
Query: 235 KYLANKNVKVWIRYVVVPGWSDDDDSAHRLG 143
+Y N+N K + RY + GW D S + +G
Sbjct: 68 QYYQNRNTKAYDRYKIPQGWLDCPPSGNEIG 98
>At1g61080.1 68414.m06877 proline-rich family protein
Length = 907
Score = 29.5 bits (63), Expect = 1.6
Identities = 14/30 (46%), Positives = 16/30 (53%)
Frame = +2
Query: 437 NSACRIASPPDAVTPPPEAFIKWRRSPLPS 526
NS+ PP PPP +FIK PLPS
Sbjct: 410 NSSQLFPPPPPPPPPPPLSFIKTASLPLPS 439
>At4g15270.1 68417.m02339 glucosyltransferase-related contains some
similarity to glucosyltransferase GI:14349251 from
[Nicotiana tabacum]
Length = 311
Score = 29.1 bits (62), Expect = 2.1
Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 5/65 (7%)
Frame = -2
Query: 280 PKSGWSFQPRTLEFAKYLANKNVKVWIRYVVV--PGWSDDDDSAHRLGEFT---RDMGNV 116
P G T+E AK L + ++WI +++ P SDDD + T +D N
Sbjct: 9 PSPGIGHLRSTVELAKRLVRSDDRLWITVIIIPYPSISDDDVETTYIASLTTASQDRLNY 68
Query: 115 EKIEL 101
E I +
Sbjct: 69 EAISV 73
>At5g03730.2 68418.m00334 serine/threonine protein kinase (CTR1)
identical to serine/threonine-protein kinase CTR1
[Arabidopsis thaliana] SWISS-PROT:Q05609
Length = 821
Score = 28.7 bits (61), Expect = 2.8
Identities = 24/70 (34%), Positives = 30/70 (42%), Gaps = 2/70 (2%)
Frame = -3
Query: 543 YR*RFDEGSGDLRHFMNASGGGVTASGGEAILQAE--FVRDWFRACKKEGIHTCLDTNGF 370
+R F G GDLR M A G ++SG Q E + A + TC D F
Sbjct: 132 FRLGFGGGGGDLRIQMAADSAGGSSSGKSWAQQTEESYQLQLALALRLSSEATCADDPNF 191
Query: 369 VRRYDPVIDE 340
+ DPV DE
Sbjct: 192 L---DPVPDE 198
>At5g03730.1 68418.m00333 serine/threonine protein kinase (CTR1)
identical to serine/threonine-protein kinase CTR1
[Arabidopsis thaliana] SWISS-PROT:Q05609
Length = 821
Score = 28.7 bits (61), Expect = 2.8
Identities = 24/70 (34%), Positives = 30/70 (42%), Gaps = 2/70 (2%)
Frame = -3
Query: 543 YR*RFDEGSGDLRHFMNASGGGVTASGGEAILQAE--FVRDWFRACKKEGIHTCLDTNGF 370
+R F G GDLR M A G ++SG Q E + A + TC D F
Sbjct: 132 FRLGFGGGGGDLRIQMAADSAGGSSSGKSWAQQTEESYQLQLALALRLSSEATCADDPNF 191
Query: 369 VRRYDPVIDE 340
+ DPV DE
Sbjct: 192 L---DPVPDE 198
>At3g50350.1 68416.m05506 expressed protein
Length = 181
Score = 28.3 bits (60), Expect = 3.7
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = +2
Query: 422 NQSRTNSACRIASPPDAVTPPPEAFIKWRRSPLPSSNL 535
NQS+TN A + P TP P + PLPS++L
Sbjct: 2 NQSKTNPAIDRKTFPPPHTPSPGVVRRIHAPPLPSTSL 39
>At1g09860.1 68414.m01110 purine permease-related low similarity to
purine permease [Arabidopsis thaliana] GI:7620007;
contains Pfam profile PF03151: Domain of unknown
function, DUF250
Length = 383
Score = 28.3 bits (60), Expect = 3.7
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = +2
Query: 227 QIFSELQRAGLETPTRFWWISSFIC 301
Q+ SE R+ LE R WWIS FIC
Sbjct: 12 QMMSENPRS-LELNQRKWWISVFIC 35
>At5g58720.1 68418.m07356 PRLI-interacting factor, putative similar
to PRLI-interacting factor N [Arabidopsis thaliana]
GI:11139276; contains Pfam profile PF01713: Smr domain
Length = 519
Score = 27.9 bits (59), Expect = 4.9
Identities = 13/42 (30%), Positives = 25/42 (59%)
Frame = -2
Query: 409 KRRHSYLSGHQRFCSSLRSGD**TAGSTRPGNARSLTDERRD 284
K+++ + +R C++ SGD G+T GN + + +ER+D
Sbjct: 2 KQKNQHKKKKKRSCAAKPSGD----GTTSDGNKKDVEEERKD 39
>At2g40475.1 68415.m04995 expressed protein
Length = 193
Score = 27.9 bits (59), Expect = 4.9
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +2
Query: 371 KPLVSRQV*MPSFLQARNQSRTNSACRIASPPDAVTPPPEAFIKWRRS 514
K L+SR V PSF + S ++S+ +SPP V P RS
Sbjct: 94 KTLLSRHVSRPSFSWSSASSSSSSSYSSSSPPSKVEHRPRKCYSCSRS 141
>At5g58400.1 68418.m07313 peroxidase, putative similar to peroxidase
[Nicotiana tabacum] gi|5381253|dbj|BAA82306
Length = 325
Score = 27.5 bits (58), Expect = 6.5
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +1
Query: 247 ACGVGNSNQILVDLVVHLLEIEHYQVGYFQQFINHRIVTTNKTV 378
A G G++N ++DL E + YF Q +NHR + T+ V
Sbjct: 233 ATGSGDNNAAILDLRTP----EKFDGSYFMQLVNHRGLLTSDQV 272
>At3g27210.1 68416.m03402 expressed protein
Length = 234
Score = 27.5 bits (58), Expect = 6.5
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = -2
Query: 283 PPKSGWSFQPRTLEF-AKYLANKNVKVWIRYVVVPGWSDDDDSAHRL-GEFTRDMGNVEK 110
P KS WSF F +K + + W++ SD DD H + G+FT +GN K
Sbjct: 64 PVKSRWSFSSSKKSFGSKDETFFDSQPWLQ-------SDSDDDFHSVNGDFTPSLGNTPK 116
>At1g71300.1 68414.m08228 Vps52/Sac2 family protein similar to
SP|P39904 SAC2 protein {Saccharomyces cerevisiae};
contains Pfam profile PF04129: Vps52 / Sac2 family
Length = 701
Score = 27.5 bits (58), Expect = 6.5
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = +1
Query: 313 HYQVGYFQQF--INHRIVTTNKTVGVQTGMNAFFFAGAEPVTNKLSL 447
H+Q Y Q F + I T+N +GV T F EP+ N+ ++
Sbjct: 286 HFQ-SYIQAFEKLQLDIATSNDLIGVDTRSTGLFSRSKEPLKNRCAV 331
>At3g29150.1 68416.m03649 expressed protein
Length = 417
Score = 27.1 bits (57), Expect = 8.6
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -3
Query: 432 RDWFRACKKEGIHTCLDTNGFVRRYDP 352
R W + G+ CL T G++R +DP
Sbjct: 218 RKWDKCVFSNGMFFCLSTCGYLRVFDP 244
>At1g72270.1 68414.m08355 expressed protein
Length = 2777
Score = 27.1 bits (57), Expect = 8.6
Identities = 20/44 (45%), Positives = 27/44 (61%), Gaps = 5/44 (11%)
Frame = +1
Query: 16 IKQLL-VMKS--AEEDAFRRDLIDSIIRGRGE--ARFSQRCPYH 132
IK+LL +MKS A E + RDL+ +IIRGR + F PY+
Sbjct: 723 IKRLLALMKSLKATETGYPRDLLLAIIRGRPSLASAFFDEFPYN 766
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,610,335
Number of Sequences: 28952
Number of extensions: 279849
Number of successful extensions: 936
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 889
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 936
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1072696904
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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