Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0505X.Seq
(443 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 139 2e-34
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 79 2e-16
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 77 9e-16
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 72 5e-14
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 58 6e-10
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 56 3e-09
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 44 8e-06
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 33 0.026
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 32 0.034
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.18
SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|... 26 3.0
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 6.9
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 6.9
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 24 9.1
SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces pomb... 24 9.1
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 24 9.1
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 24 9.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 139 bits (337), Expect = 2e-34
Identities = 61/69 (88%), Positives = 68/69 (98%)
Frame = +2
Query: 50 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDKAQSK 229
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGD+AQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 230 RGILTLKYP 256
RGILTLKYP
Sbjct: 62 RGILTLKYP 70
Score = 131 bits (317), Expect = 4e-32
Identities = 55/62 (88%), Positives = 59/62 (95%)
Frame = +1
Query: 253 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFNS 432
PIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FE FN+
Sbjct: 70 PIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNA 129
Query: 433 PA 438
PA
Sbjct: 130 PA 131
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 79.4 bits (187), Expect = 2e-16
Identities = 33/61 (54%), Positives = 40/61 (65%)
Frame = +1
Query: 247 EVPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENF 426
+ PI HG I NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+F
Sbjct: 91 DYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESF 150
Query: 427 N 429
N
Sbjct: 151 N 151
Score = 44.4 bits (100), Expect = 8e-06
Identities = 21/52 (40%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +2
Query: 68 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDK 217
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 77.4 bits (182), Expect = 9e-16
Identities = 34/67 (50%), Positives = 47/67 (70%)
Frame = +2
Query: 56 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDKAQSKRG 235
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG +AQ+ RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 236 ILTLKYP 256
+L ++ P
Sbjct: 68 LLKIQRP 74
Score = 73.3 bits (172), Expect = 2e-14
Identities = 32/64 (50%), Positives = 43/64 (67%), Gaps = 1/64 (1%)
Frame = +1
Query: 253 PIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFN 429
PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +E N
Sbjct: 74 PIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLN 133
Query: 430 SPAM 441
PA+
Sbjct: 134 VPAL 137
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 71.7 bits (168), Expect = 5e-14
Identities = 27/58 (46%), Positives = 44/58 (75%)
Frame = +1
Query: 253 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENF 426
P+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE +
Sbjct: 70 PMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERY 127
Score = 62.9 bits (146), Expect = 2e-11
Identities = 32/67 (47%), Positives = 44/67 (65%), Gaps = 2/67 (2%)
Frame = +2
Query: 62 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDKAQSKRG 235
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGD+A++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 236 ILTLKYP 256
+L +KYP
Sbjct: 64 LLQVKYP 70
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 58.0 bits (134), Expect = 6e-10
Identities = 25/63 (39%), Positives = 41/63 (65%)
Frame = +1
Query: 253 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFNS 432
P E +I+NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N+
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 433 PAM 441
P++
Sbjct: 145 PSV 147
Score = 36.7 bits (81), Expect = 0.002
Identities = 17/42 (40%), Positives = 27/42 (64%)
Frame = +2
Query: 38 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 163
F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 55.6 bits (128), Expect = 3e-09
Identities = 24/61 (39%), Positives = 34/61 (55%)
Frame = +1
Query: 256 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFNSP 435
I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MFE+ P
Sbjct: 69 IRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCP 128
Query: 436 A 438
A
Sbjct: 129 A 129
Score = 40.7 bits (91), Expect = 1e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +2
Query: 53 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 154
D+V+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 44.4 bits (100), Expect = 8e-06
Identities = 18/59 (30%), Positives = 34/59 (57%)
Frame = +1
Query: 262 HGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFNSPA 438
+GII +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPA 132
Score = 34.7 bits (76), Expect = 0.006
Identities = 17/57 (29%), Positives = 33/57 (57%)
Frame = +2
Query: 53 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDKAQ 223
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV D+ Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 32.7 bits (71), Expect = 0.026
Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 4/70 (5%)
Frame = +1
Query: 238 PHSEVPIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMT 405
P+ PI+ G + +W+ ++ W H Y+ L P + +PV L +RE T
Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166
Query: 406 QIMFENFNSP 435
Q FE P
Sbjct: 167 QFFFEECQVP 176
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 32.3 bits (70), Expect = 0.034
Identities = 16/53 (30%), Positives = 29/53 (54%)
Frame = +2
Query: 113 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDKAQSKRGILTLKYPSSTVS 271
G+ PRA F ++ P H G+++ M KD G++ S +G + + P + +S
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTPENEIS 552
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.9 bits (64), Expect = 0.18
Identities = 14/43 (32%), Positives = 26/43 (60%), Gaps = 2/43 (4%)
Frame = +1
Query: 313 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFENFNSP 435
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 25.8 bits (54), Expect = 3.0
Identities = 14/47 (29%), Positives = 29/47 (61%), Gaps = 3/47 (6%)
Frame = +1
Query: 250 VPIEHGIITNWDDME-KIWH--HTFYNELRVAPEEHPVLLTEAPLNP 381
VP + +I + DD +W+ HTF+++ + P EH + ++++ +NP
Sbjct: 265 VPQGNFVIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 24.6 bits (51), Expect = 6.9
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +2
Query: 68 LVVDNGSGMCKAGFAG 115
+V+DNG+ KAGFAG
Sbjct: 8 IVLDNGAYHIKAGFAG 23
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 24.6 bits (51), Expect = 6.9
Identities = 9/21 (42%), Positives = 11/21 (52%)
Frame = +1
Query: 229 ERYPHSEVPIEHGIITNWDDM 291
E YP E PI+ I W+ M
Sbjct: 1369 EHYPSGEFPIDRKFILYWEPM 1389
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 24.2 bits (50), Expect = 9.1
Identities = 9/25 (36%), Positives = 17/25 (68%)
Frame = +1
Query: 355 LLTEAPLNPKANREKMTQIMFENFN 429
+L APL+ + +KM +++FE +N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC16C4.09 |sts5|orb4|RNB-like protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1066
Score = 24.2 bits (50), Expect = 9.1
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -1
Query: 260 SMGTSE*GYLSCSGPCHPRRSLSGPYRP 177
S+G+S G+ S G +PR++L PY P
Sbjct: 266 SVGSSGSGF-SSGGSGNPRKNLFSPYLP 292
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 24.2 bits (50), Expect = 9.1
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -3
Query: 156 RPTMDGKTARGASSPAKPALHMPEPL 79
RP+ DG+ A G A P++ P P+
Sbjct: 950 RPSNDGRKASGPRPAAPPSIPPPLPV 975
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 24.2 bits (50), Expect = 9.1
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +2
Query: 173 VMVGMGQKDSYVGDKAQSKRGILTLK 250
+M+G +Y+G AQ KR +LT +
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTAR 190
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,869,887
Number of Sequences: 5004
Number of extensions: 39725
Number of successful extensions: 149
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 162176800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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