Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0499.Seq
(633 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 124 8e-30
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 80 3e-16
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 69 6e-13
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 68 1e-12
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 44 2e-05
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 38 0.001
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 38 0.002
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 36 0.005
SPAC16E8.07c |vph1||V-type ATPase subunit a|Schizosaccharomyces ... 29 0.42
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 28 1.3
SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomy... 27 2.3
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 26 5.2
SPCC663.10 |||methyltransferase, DUF1613 family |Schizosaccharom... 26 5.2
SPAC13G7.08c |crb3||WD repeat protein Crb3|Schizosaccharomyces p... 25 6.9
SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 25 9.1
SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces p... 25 9.1
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 124 bits (300), Expect = 8e-30
Identities = 59/79 (74%), Positives = 67/79 (84%)
Frame = +2
Query: 257 AEIMFESFNVPGLYIAVQAVLALAASWKSRTSAERTFTGIVVDSGDGVTHIVPVAEGYVI 436
AEIMFESFN GLYIAVQAVLALAASW S +R+ TG VVDSGDGVTHI+PVAEGYVI
Sbjct: 143 AEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVI 202
Query: 437 GSCIKHIPIAGRNITSFIQ 493
GS IK +P+AGR++T F+Q
Sbjct: 203 GSSIKTMPLAGRDVTYFVQ 221
Score = 113 bits (273), Expect = 2e-26
Identities = 48/74 (64%), Positives = 62/74 (83%), Gaps = 2/74 (2%)
Frame = +3
Query: 36 KAVEDLDFFIGDEAFE--ATGYSVKYPVRHGIVEDWDLMERYIEQCVFKYLRAEPEDHHF 209
+A EDLDFFIG++A + + GYS+ YP+RHG +E+WD MER+ +Q +FKYLR EPEDH+F
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 210 LMTEPPLNTPENRE 251
L+TEPPLN PENRE
Sbjct: 127 LLTEPPLNPPENRE 140
Score = 49.6 bits (113), Expect = 4e-07
Identities = 21/52 (40%), Positives = 35/52 (67%)
Frame = +1
Query: 478 YFIYSIITP*REVGIPPEQSLETAKAIKAKYSYICPDIAKEFAKYDSDPGKW 633
YF+ S++ E P+ SL+TA+ IK + Y+CPDI KEF+++D +P ++
Sbjct: 218 YFVQSLLRDRNE----PDSSLKTAERIKEECCYVCPDIVKEFSRFDREPDRY 265
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 79.8 bits (188), Expect = 3e-16
Identities = 37/79 (46%), Positives = 55/79 (69%)
Frame = +2
Query: 254 LAEIMFESFNVPGLYIAVQAVLALAASWKSRTSAERTFTGIVVDSGDGVTHIVPVAEGYV 433
+ +I+FE+FN P Y+A+QAVL+L AS ++ TGIV+DSGDGVTH VP+ EGY
Sbjct: 119 MTQIIFETFNAPAFYVAIQAVLSLYASGRT--------TGIVLDSGDGVTHTVPIYEGYA 170
Query: 434 IGSCIKHIPIAGRNITSFI 490
+ I + +AGR++T ++
Sbjct: 171 LPHAIMRLDLAGRDLTDYL 189
Score = 68.1 bits (159), Expect = 1e-12
Identities = 31/67 (46%), Positives = 41/67 (61%), Gaps = 1/67 (1%)
Frame = +3
Query: 54 DFFIGDEAFEATGY-SVKYPVRHGIVEDWDLMERYIEQCVFKYLRAEPEDHHFLMTEPPL 230
D ++GDEA G ++KYP+ HGIV +WD ME+ + LR PE+H L+TE PL
Sbjct: 51 DSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPL 110
Query: 231 NTPENRE 251
N NRE
Sbjct: 111 NPKSNRE 117
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 68.9 bits (161), Expect = 6e-13
Identities = 35/79 (44%), Positives = 50/79 (63%)
Frame = +2
Query: 254 LAEIMFESFNVPGLYIAVQAVLALAASWKSRTSAERTFTGIVVDSGDGVTHIVPVAEGYV 433
+ E MFE + G+Y+A+QAVL+L A S +G+VVDSGDGVTHIVPV E V
Sbjct: 119 MCETMFERYGFGGVYVAIQAVLSLYAQGLS--------SGVVVDSGDGVTHIVPVYESVV 170
Query: 434 IGSCIKHIPIAGRNITSFI 490
+ + + +AGR+ T ++
Sbjct: 171 LNHLVGRLDVAGRDATRYL 189
Score = 54.8 bits (126), Expect = 1e-08
Identities = 27/74 (36%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Frame = +3
Query: 33 TKAVEDLDFFIGDEAFEATGY-SVKYPVRHGIVEDWDLMERYIEQCVFKYLRAEPEDHHF 209
T V+ D +GDEA VKYP+ +GI+ D++ M + + F+ L+ +P
Sbjct: 44 TGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKI 103
Query: 210 LMTEPPLNTPENRE 251
L+TEPP+N NRE
Sbjct: 104 LLTEPPMNPVANRE 117
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 68.1 bits (159), Expect = 1e-12
Identities = 37/85 (43%), Positives = 55/85 (64%)
Frame = +2
Query: 239 RKQRILAEIMFESFNVPGLYIAVQAVLALAASWKSRTSAERTFTGIVVDSGDGVTHIVPV 418
R + +AE +E+ NVP L ++Q VLAL AS +RT TGIV++ GDG+TH VP+
Sbjct: 119 RNKEKIAEYFYETLNVPALSFSLQPVLALYAS--ART------TGIVLECGDGLTHSVPI 170
Query: 419 AEGYVIGSCIKHIPIAGRNITSFIQ 493
+G+ I S I+ I GR++T ++Q
Sbjct: 171 YDGFSIPSAIQQEEIGGRDVTDYLQ 195
Score = 48.0 bits (109), Expect = 1e-06
Identities = 28/85 (32%), Positives = 43/85 (50%), Gaps = 3/85 (3%)
Frame = +3
Query: 6 VGDQSTRRMTKAVEDLDFFIGDEAFEATGY-SVKYPVRHGIVEDWDLMERYIEQCVF--K 176
VG R+ + D F+G EA G ++ P+ GI+++W ME I ++ +
Sbjct: 39 VGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEE-IWSYIYSDQ 97
Query: 177 YLRAEPEDHHFLMTEPPLNTPENRE 251
L PE+H L+TEPPL N+E
Sbjct: 98 QLNTLPEEHPLLLTEPPLANIRNKE 122
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 44.0 bits (99), Expect = 2e-05
Identities = 23/68 (33%), Positives = 35/68 (51%)
Frame = +3
Query: 90 GYSVKYPVRHGIVEDWDLMERYIEQCVFKYLRAEPEDHHFLMTEPPLNTPENREY*PKSC 269
G +K +R+G VE+WD+ + + L+ P +H L+TEP N PENR ++
Sbjct: 62 GMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETM 121
Query: 270 LSHLMCQA 293
L C A
Sbjct: 122 FESLRCPA 129
Score = 28.3 bits (60), Expect = 0.97
Identities = 19/59 (32%), Positives = 27/59 (45%)
Frame = +2
Query: 260 EIMFESFNVPGLYIAVQAVLALAASWKSRTSAERTFTGIVVDSGDGVTHIVPVAEGYVI 436
E MFES P Y+A Q A AS K T +VD G + + + +G+V+
Sbjct: 119 ETMFESLRCPATYLAKQETCAAFASGKG--------TACLVDIGAERSSVSAIYDGFVL 169
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 38.3 bits (85), Expect = 0.001
Identities = 21/79 (26%), Positives = 40/79 (50%)
Frame = +2
Query: 254 LAEIMFESFNVPGLYIAVQAVLALAASWKSRTSAERTFTGIVVDSGDGVTHIVPVAEGYV 433
+ E++FE +N P + + + + + K +S GIV++ G+ +H++PV G
Sbjct: 134 MTELLFELYNAPSVAYGIDGLFSFYHNTKPSSS------GIVLNLGNAASHVIPVLNGER 187
Query: 434 IGSCIKHIPIAGRNITSFI 490
I S K I G +S++
Sbjct: 188 ILSEAKRISWGGSQSSSYL 206
Score = 32.3 bits (70), Expect = 0.060
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +3
Query: 69 DEAFEATGYSV-KYPVRHGIVEDWDLMERYIEQCVFKYLRAEPEDHHFLMTEPPLN 233
D E S+ + P ++ +WDLME+ ++ K L + +H MTEP N
Sbjct: 72 DTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFLK-LGIDRMEHPICMTEPLAN 126
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 37.5 bits (83), Expect = 0.002
Identities = 27/86 (31%), Positives = 42/86 (48%)
Frame = +2
Query: 236 SRKQRILAEIMFESFNVPGLYIAVQAVLALAASWKSRTSAERTFTGIVVDSGDGVTHIVP 415
S +Q+I+ E FE +VP Y+ QAV A+ KS T ++VD G + P
Sbjct: 115 SVRQQIM-EAAFEQLHVPAFYLTKQAVCVAFANSKS--------TALIVDIGSDNASVTP 165
Query: 416 VAEGYVIGSCIKHIPIAGRNITSFIQ 493
V +G +I I +AG + + I+
Sbjct: 166 VVDGLIIRKGIFKQSLAGDFLNANIE 191
Score = 34.3 bits (75), Expect = 0.015
Identities = 25/95 (26%), Positives = 42/95 (44%)
Frame = +3
Query: 90 GYSVKYPVRHGIVEDWDLMERYIEQCVFKYLRAEPEDHHFLMTEPPLNTPENREY*PKSC 269
G VK +GI++DW+ E+ + + L+ P ++ ++TEP N P+S
Sbjct: 65 GMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWN--------PQSV 116
Query: 270 LSHLMCQAFTLLYKPFLLWLRHGSHVRLLNAPSLA 374
+M AF L+ P + V N+ S A
Sbjct: 117 RQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTA 151
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 35.9 bits (79), Expect = 0.005
Identities = 13/40 (32%), Positives = 26/40 (65%)
Frame = +2
Query: 374 IVVDSGDGVTHIVPVAEGYVIGSCIKHIPIAGRNITSFIQ 493
+V+DSG THI+PV + V +K + + G+ +T++++
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLK 198
>SPAC16E8.07c |vph1||V-type ATPase subunit a|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 805
Score = 29.5 bits (63), Expect = 0.42
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 270 LSHLMCQAFTLLYKPFLLWLRHGS 341
++ L+C + L+ KPF+LW RH +
Sbjct: 627 IAALICVPWLLIVKPFVLWRRHSN 650
>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 452
Score = 27.9 bits (59), Expect = 1.3
Identities = 12/48 (25%), Positives = 26/48 (54%)
Frame = -3
Query: 439 SNNISFCNWNNVSNAISAVYYDASEGAFSRRT*LP*RSQSKNGLYSNV 296
S+N ++ WN+ S+ S+V+ + + + S + +P S + Y N+
Sbjct: 348 SSNTTYFYWNSTSSLSSSVFTNTTSSSNSTNSSIPTTYPSNSTTYQNI 395
>SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 300
Score = 27.1 bits (57), Expect = 2.3
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +2
Query: 548 PKLLKQNTVISVLTLLKNLPNTIAILV 628
PK LK + VLT L+NLP + V
Sbjct: 67 PKFLKNQVFMEVLTALQNLPGMALLTV 93
>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 541
Score = 25.8 bits (54), Expect = 5.2
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +1
Query: 472 KHYFIYSIITP*REVGIPPEQSLETAK 552
K YF++S + P + + +PPE + T K
Sbjct: 142 KFYFVHSYMIPEKGLILPPEFKIATTK 168
>SPCC663.10 |||methyltransferase, DUF1613 family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 5.2
Identities = 14/50 (28%), Positives = 25/50 (50%), Gaps = 2/50 (4%)
Frame = +2
Query: 287 PGLYIAVQAVLA--LAASWKSRTSAERTFTGIVVDSGDGVTHIVPVAEGY 430
PG ++ +A L WK S+ + F+ + V G+G+ + + EGY
Sbjct: 249 PGKHVFEDLAIAAFLIELWKQTYSSNKEFSFVDVGCGNGLLVYLLLMEGY 298
>SPAC13G7.08c |crb3||WD repeat protein Crb3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 446
Score = 25.4 bits (53), Expect = 6.9
Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
Frame = +2
Query: 179 SPCGTRRSSFLNDRASAQHSRKQRIL----AEIMFESFNVPGLYIAVQA 313
SP + LN SAQH+R Q + EI+ +S +P + I VQ+
Sbjct: 36 SPAKNATCTTLNHLLSAQHTRPQLNIHNFGKEILDQSIILPEILICVQS 84
>SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 661
Score = 25.0 bits (52), Expect = 9.1
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -1
Query: 465 AIGMCFIHDPITYPS 421
A+G FIH ITYPS
Sbjct: 429 AVGPSFIHKVITYPS 443
>SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 832
Score = 25.0 bits (52), Expect = 9.1
Identities = 11/39 (28%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = +3
Query: 54 DFFIGDEAFEATGYSVKYPVRHGI-VEDWDLMERYIEQC 167
D F+G + +EA V +P +G+ ++L Y++ C
Sbjct: 683 DVFVGYKYYEAVEREVLFPFGYGLSYTTFELSNLYLKNC 721
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,626,747
Number of Sequences: 5004
Number of extensions: 52950
Number of successful extensions: 144
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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