Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0490.Seq
(773 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 31 1.1
At3g29580.1 68416.m03717 hypothetical protein weak similarity to... 29 2.6
At3g08870.1 68416.m01031 lectin protein kinase, putative similar... 29 2.6
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 29 2.6
At4g02570.1 68417.m00351 cullin family protein similar to cullin... 29 4.5
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 28 6.0
At4g05560.1 68417.m00847 hypothetical protein 28 6.0
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 28 6.0
At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident... 28 7.9
At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly... 28 7.9
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 30.7 bits (66), Expect = 1.1
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = +3
Query: 534 SVVDTKFRVRNVTGLRVMDASIMPSVIRGNTNAPSIMIGE-KGADMIKKH 680
SVV+ +++V V LRV+D S N A +M+G +G ++K+H
Sbjct: 454 SVVNEEYKVNGVKRLRVVDGSTFEESPGTNPMATVLMLGRYQGIKILKEH 503
>At3g29580.1 68416.m03717 hypothetical protein weak similarity to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471
Length = 306
Score = 29.5 bits (63), Expect = 2.6
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 358 GETKAFKESGFKLEWIELDACKSFD-KNSDEFLECIAREI 474
G ++ KE+GFKL+W+E + + K +E E REI
Sbjct: 243 GLLRSMKEAGFKLDWLEKKLNEVLEKKEKEESYETRMREI 282
>At3g08870.1 68416.m01031 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 693
Score = 29.5 bits (63), Expect = 2.6
Identities = 22/78 (28%), Positives = 35/78 (44%)
Frame = -1
Query: 239 YPQIWVSTKYCKRAWCICSRSFYGTYRGLLRLNRAYGTCPISCRSLEGILSSGTACQKQE 60
Y ++++TK K + I + F YRG L + I+ SL+G+ + E
Sbjct: 358 YRDLYLATKKFKESEIIGTGGFGIVYRGNLSSSGPIAVKKITSNSLQGVRE---FMAEIE 414
Query: 59 SSGRLCFRKR*LCVGWCR 6
S GRL + GWC+
Sbjct: 415 SLGRLGHKNLVNLQGWCK 432
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 29.5 bits (63), Expect = 2.6
Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +3
Query: 534 SVVDTKFRVRNVTGLRVMDASIMPSVIRGNTNAPSIMIGE-KGADMIKK 677
SVVD +++V V LRV+D S N A +M+G +G ++K+
Sbjct: 434 SVVDEEYKVNGVKRLRVVDGSTFEESPGTNPMATVLMLGRYQGIKILKE 482
>At4g02570.1 68417.m00351 cullin family protein similar to cullin 3
[Homo sapiens] GI:3639052; contains Pfam profile
PF00888: Cullin family
Length = 738
Score = 28.7 bits (61), Expect = 4.5
Identities = 12/43 (27%), Positives = 25/43 (58%)
Frame = +2
Query: 56 NSPASDMQYHYLIFPPSSYNLLDMFRKHGLSEEVHDKFRKMNE 184
N PA D + + +++ + YN+ H S++++DK+R+ E
Sbjct: 30 NEPAFDSEQYMMLYT-TIYNMCTQKPPHDYSQQLYDKYREAFE 71
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/49 (28%), Positives = 27/49 (55%)
Frame = +1
Query: 235 G*IVIEDKNPFDKPLLYADYYKDIEDLYTVIRAFKQHSLRLGETKAFKE 381
G + + + NP D P + +YY++ EDL ++ +R+ +KAF +
Sbjct: 441 GHMKLRNTNPRDNPSVTFNYYQEPEDLNKCVKGLNT-IIRMINSKAFSK 488
Score = 27.9 bits (59), Expect = 7.9
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +3
Query: 537 VVDTKFRVRNVTGLRVMDASIMPSVIRGNTNAPSIMIG 650
VVD ++V + GLRV+D S N A +M+G
Sbjct: 544 VVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLG 581
>At4g05560.1 68417.m00847 hypothetical protein
Length = 104
Score = 28.3 bits (60), Expect = 6.0
Identities = 22/91 (24%), Positives = 38/91 (41%), Gaps = 2/91 (2%)
Frame = +2
Query: 11 TSPHRVIAFENTTDPNSPASDMQYHYLI--FPPSSYNLLDMFRKHGLSEEVHDKFRKMNE 184
++P +F+ +TD ++P S +HY P LLD+ G + +
Sbjct: 5 STPRSSRSFKISTDHSTPRSSRGHHYFTTSLDPEVEPLLDLITLPGSRVSPSPPLDCILD 64
Query: 185 NKYTMLVYNTLLKPKSAGRLLLKTRIHSTSH 277
+K L+Y+ L + IHSTS+
Sbjct: 65 DKLQSLLYSALNQTLEHKEEKKTPAIHSTSY 95
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 28.3 bits (60), Expect = 6.0
Identities = 14/47 (29%), Positives = 28/47 (59%)
Frame = +1
Query: 235 G*IVIEDKNPFDKPLLYADYYKDIEDLYTVIRAFKQHSLRLGETKAF 375
G + ++ +NP D P++ +Y++ +DL +R Q R+ ++KAF
Sbjct: 413 GHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGI-QTIERVVQSKAF 458
>At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b)
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50580;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 27.9 bits (59), Expect = 7.9
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +1
Query: 400 WIELDACKSFDKNSDEFLECIAREITFSLYHPTSTVRWGPMVTR 531
+++L K K DE +EC E+TF + +V W PM R
Sbjct: 166 FVDLQNYKYTKKKLDETVEC---ELTFPSFEEAVSVPWKPMPRR 206
>At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 27.9 bits (59), Expect = 7.9
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +1
Query: 400 WIELDACKSFDKNSDEFLECIAREITFSLYHPTSTVRWGPMVTR 531
+++L K K DE +EC E+TF + +V W PM R
Sbjct: 166 FVDLQNYKYTKKKLDETVEC---ELTFPSFEEAVSVPWKPMPRR 206
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,178,595
Number of Sequences: 28952
Number of extensions: 377727
Number of successful extensions: 1070
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1037
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1070
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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