BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0417.Seq (775 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g47880.1 68418.m05915 eukaryotic peptide chain release factor... 144 8e-35 At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot... 142 2e-34 At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot... 136 1e-32 At1g31510.1 68414.m03863 F-box family protein-related contains w... 30 2.0 At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ... 29 2.6 At4g14480.1 68417.m02233 protein kinase family protein contains ... 29 4.5 At5g56240.1 68418.m07018 expressed protein 28 7.9 At3g59610.1 68416.m06651 F-box family protein / jacalin lectin f... 28 7.9 >At5g47880.1 68418.m05915 eukaryotic peptide chain release factor subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic peptide chain release factor subunit 1-1 (eRF1-1) (Eukaryotic release factor 1-1) (Omnipotent suppressor protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis thaliana}, eukaryotic release factor 1 homolog GI:1155261 from [Arabidopsis thaliana]; contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 436 Score = 144 bits (348), Expect = 8e-35 Identities = 67/84 (79%), Positives = 72/84 (85%) Frame = +3 Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689 IKSRVNR SVLGAITS Q RLKLY +VPPNGLV+Y GTIV E+GKEKKV IDFEPF+PIN Sbjct: 61 IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120 Query: 690 TSLYLCDNKFHTEALTALLADDNK 761 SLYLCDNKFHTEAL LL D+K Sbjct: 121 ASLYLCDNKFHTEALNELLESDDK 144 Score = 99 bits (238), Expect = 2e-21 Identities = 47/65 (72%), Positives = 57/65 (87%), Gaps = 1/65 (1%) Frame = +1 Query: 331 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS- 507 M +++ D+N+EIWKIKKLIKSLE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTAS Sbjct: 1 MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60 Query: 508 ISSHV 522 I S V Sbjct: 61 IKSRV 65 >At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 435 Score = 142 bits (345), Expect = 2e-34 Identities = 67/84 (79%), Positives = 71/84 (84%) Frame = +3 Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689 IKSRVNR SVL AITS Q RLKLY KVPPNGLV+Y GTIVT++GKEKKV IDFEPFKPIN Sbjct: 60 IKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPIN 119 Query: 690 TSLYLCDNKFHTEALTALLADDNK 761 SLYLCDNKFHTE L LL D+K Sbjct: 120 ASLYLCDNKFHTEPLNELLESDDK 143 Score = 97.1 bits (231), Expect = 1e-20 Identities = 45/62 (72%), Positives = 55/62 (88%), Gaps = 1/62 (1%) Frame = +1 Query: 340 ESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-ISS 516 + +D+N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ++RV+KMLADE+GTAS I S Sbjct: 3 DQESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASNIKS 62 Query: 517 HV 522 V Sbjct: 63 RV 64 >At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 434 Score = 136 bits (330), Expect = 1e-32 Identities = 64/84 (76%), Positives = 69/84 (82%) Frame = +3 Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689 IKSRVNR SVL AITS Q RLKLY KVP NGLV+Y GTIV ++GKEKKV DFEPF+PIN Sbjct: 59 IKSRVNRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPIN 118 Query: 690 TSLYLCDNKFHTEALTALLADDNK 761 SLYLCDNKFHTEAL LL D+K Sbjct: 119 ASLYLCDNKFHTEALNELLESDDK 142 Score = 97.1 bits (231), Expect = 1e-20 Identities = 49/65 (75%), Positives = 56/65 (86%), Gaps = 1/65 (1%) Frame = +1 Query: 331 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS- 507 M+EE AD N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTAS Sbjct: 1 MAEE--ADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 58 Query: 508 ISSHV 522 I S V Sbjct: 59 IKSRV 63 >At1g31510.1 68414.m03863 F-box family protein-related contains weak hit to TIGRFAM TIGR01640 : F-box protein interaction domain; ; similar to F-box protein family, AtFBX9 (GI:20197985) [Arabidopsis thaliana] Length = 309 Score = 29.9 bits (64), Expect = 2.0 Identities = 14/40 (35%), Positives = 24/40 (60%) Frame = +3 Query: 651 KVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKLVS 770 K +++F+ FKP+++S L N FH + L ++ LVS Sbjct: 38 KPSLNFQVFKPLSSSDLLEHNMFHCDGLLLMVMGTKILVS 77 >At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains similarity to NAD kinase [Homo sapiens] gi|20070086|gb|AAM01195; contains Pfam domain, PF01513: ATP-NAD kinase Length = 985 Score = 29.5 bits (63), Expect = 2.6 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +1 Query: 391 KSLEMARGNGTSMISLIIPPKDQISR 468 KS+ RGNG S + +PP D +SR Sbjct: 561 KSISDNRGNGFSAAPIAVPPSDNLSR 586 >At4g14480.1 68417.m02233 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 487 Score = 28.7 bits (61), Expect = 4.5 Identities = 16/54 (29%), Positives = 30/54 (55%) Frame = +1 Query: 322 RNKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKML 483 +N+ E D+++ + K+K L KSLE R + +I + K++ SR ++L Sbjct: 407 KNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELL 460 >At5g56240.1 68418.m07018 expressed protein Length = 986 Score = 27.9 bits (59), Expect = 7.9 Identities = 17/48 (35%), Positives = 24/48 (50%) Frame = +3 Query: 342 IVGRSKCRNMEDKETYKEFGNGQRKWNIDDIPDHST*GSNLESIKDVG 485 I G++ C+ ED+ E G G +D IP GS L S +D+G Sbjct: 668 IYGQTSCK--EDERFDSEVGQGSLFVEVDTIPIPGPPGSFLPSPRDMG 713 >At3g59610.1 68416.m06651 F-box family protein / jacalin lectin family protein contains Pfam profile PF01419: Jacalin-like lectin domain Length = 521 Score = 27.9 bits (59), Expect = 7.9 Identities = 12/45 (26%), Positives = 25/45 (55%) Frame = +1 Query: 331 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQIS 465 + +E RN+EIW K+ IK+ + +++S+++P +S Sbjct: 251 LEQEIYNTRNIEIWVTKETIKNGDGEAVEWVNLMSVLVPEWSSLS 295 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,609,143 Number of Sequences: 28952 Number of extensions: 340307 Number of successful extensions: 907 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 872 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 907 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1726528800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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