Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0416.Seq
(774 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic... 185 1e-45
UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor... 176 6e-43
UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;... 126 6e-28
UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor... 123 4e-27
UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=... 119 9e-26
UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor... 114 2e-24
UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative; ... 106 5e-22
UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release fa... 90 5e-17
UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor... 81 3e-14
UniRef50_P58227 Cluster: Peptide chain release factor subunit 1;... 76 9e-13
UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2; Cr... 75 2e-12
UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus ... 75 3e-12
UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;... 61 3e-08
UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;... 61 3e-08
UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;... 58 2e-07
UniRef50_Q9YAF1 Cluster: Peptide chain release factor subunit 1;... 56 1e-06
UniRef50_Q46FF0 Cluster: Peptide chain release factor, subunit 1... 55 2e-06
UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:... 54 5e-06
UniRef50_Q8ZU81 Cluster: Peptide chain release factor subunit 1;... 53 9e-06
UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-... 50 6e-05
UniRef50_UPI000150A1F7 Cluster: eRF1 domain 1 family protein; n=... 43 0.010
UniRef50_Q3UUT4 Cluster: 16 days neonate thymus cDNA, RIKEN full... 35 2.6
UniRef50_Q97DK8 Cluster: Uncharacterized conserved protein, phnB... 35 2.6
UniRef50_A2DQW1 Cluster: DHHC zinc finger domain containing prot... 33 6.0
UniRef50_UPI00006CC094 Cluster: hypothetical protein TTHERM_0021... 33 7.9
UniRef50_Q8I368 Cluster: Putative uncharacterized protein PFI040... 33 7.9
>UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic
release factor 1 CG5605-PA, isoform A; n=1; Apis
mellifera|Rep: PREDICTED: similar to eukaryotic release
factor 1 CG5605-PA, isoform A - Apis mellifera
Length = 265
Score = 185 bits (450), Expect = 1e-45
Identities = 87/88 (98%), Positives = 87/88 (98%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN
Sbjct: 62 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 121
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
TSLYLCDNKFHTEALTALLADDNK GFI
Sbjct: 122 TSLYLCDNKFHTEALTALLADDNKFGFI 149
Score = 116 bits (278), Expect = 9e-25
Identities = 59/64 (92%), Positives = 61/64 (95%), Gaps = 1/64 (1%)
Frame = +1
Query: 334 SEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-I 510
+EE+SADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS I
Sbjct: 3 NEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNI 62
Query: 511 SSHV 522
S V
Sbjct: 63 KSRV 66
>UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=137; Eukaryota|Rep: Eukaryotic peptide
chain release factor subunit 1 - Homo sapiens (Human)
Length = 437
Score = 176 bits (428), Expect = 6e-43
Identities = 82/88 (93%), Positives = 85/88 (96%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKSRVNRLSVLGAITSVQ RLKLY KVPPNGLV+YCGTIVTEEGKEKKVNIDFEPFKPIN
Sbjct: 62 IKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPIN 121
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
TSLYLCDNKFHTEALTALL+DD+K GFI
Sbjct: 122 TSLYLCDNKFHTEALTALLSDDSKFGFI 149
Score = 108 bits (259), Expect = 2e-22
Identities = 55/63 (87%), Positives = 58/63 (92%), Gaps = 1/63 (1%)
Frame = +1
Query: 337 EESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-IS 513
+ S+ADRNVEIWKIKKLIKSLE ARGNGTSMISLIIPPKDQISRV+KMLADEFGTAS I
Sbjct: 4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIK 63
Query: 514 SHV 522
S V
Sbjct: 64 SRV 66
>UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;
n=1; Encephalitozoon cuniculi|Rep: PEPTIDE CHAIN RELEASE
FACTOR SUBUNIT 1 - Encephalitozoon cuniculi
Length = 386
Score = 126 bits (304), Expect = 6e-28
Identities = 55/88 (62%), Positives = 71/88 (80%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKSRVNRLSVLGAITS Q +LK Y+K PPNGL I+ GT++T++ KEKK+++ EP KP+N
Sbjct: 55 IKSRVNRLSVLGAITSAQSKLKQYSKTPPNGLGIFVGTVLTDQNKEKKISVGIEPIKPVN 114
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
TSLY+CD+KFH + L AL D+ + GFI
Sbjct: 115 TSLYMCDSKFHVDDLLALFEDEARYGFI 142
Score = 84.2 bits (199), Expect = 3e-15
Identities = 40/55 (72%), Positives = 49/55 (89%), Gaps = 1/55 (1%)
Frame = +1
Query: 361 VEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-ISSHV 522
VE+W++KKL+++L +RGNGTSMIS+IIPPKDQISRVSKML DE+GTAS I S V
Sbjct: 5 VEMWRMKKLLRNLRDSRGNGTSMISIIIPPKDQISRVSKMLTDEYGTASNIKSRV 59
>UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=6; Trypanosomatidae|Rep: Eukaryotic peptide
chain release factor subunit 1 - Trypanosoma brucei
brucei
Length = 452
Score = 123 bits (297), Expect = 4e-27
Identities = 54/88 (61%), Positives = 67/88 (76%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKS N+LSV AIT+ RLK Y ++PPNGL++YCGT++T E KEKK+ +D EPFKP++
Sbjct: 63 IKSHTNKLSVQSAITASLGRLKQYNRLPPNGLLLYCGTVLTAENKEKKLTLDIEPFKPVS 122
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
SLYLCDNKFHTE L +L D K GFI
Sbjct: 123 RSLYLCDNKFHTEELHRMLESDEKFGFI 150
Score = 62.5 bits (145), Expect = 1e-08
Identities = 32/62 (51%), Positives = 46/62 (74%), Gaps = 1/62 (1%)
Frame = +1
Query: 337 EESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-IS 513
E S +++ +E +K+KKLI+ LE ARG GTS+IS+ + PK+QIS + L +E+GTAS I
Sbjct: 5 ELSESEKAIERYKVKKLIQMLESARGMGTSVISIYMTPKEQISGMVTKLNNEYGTASNIK 64
Query: 514 SH 519
SH
Sbjct: 65 SH 66
>UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=1;
Guillardia theta|Rep: Eukaryotic release factor 1
homolog - Guillardia theta (Cryptomonas phi)
Length = 409
Score = 119 bits (286), Expect = 9e-26
Identities = 52/88 (59%), Positives = 65/88 (73%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKSR+NR SVL AI+SVQ +LK YTK P NGL+IYCGT E KEKK+ I+ EP KP+N
Sbjct: 58 IKSRINRSSVLSAISSVQQKLKYYTKTPNNGLIIYCGTFTDENNKEKKILIEIEPLKPLN 117
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
+S Y+CD+KFHT L ++ D K+ FI
Sbjct: 118 SSFYICDSKFHTNILLEMIQVDQKIAFI 145
Score = 66.1 bits (154), Expect = 9e-10
Identities = 30/48 (62%), Positives = 37/48 (77%)
Frame = +1
Query: 364 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 507
E+WKIKK + L +GNGTSMISL+IP + IS+VSKML+DE TAS
Sbjct: 9 ELWKIKKTYQYLSTIKGNGTSMISLVIPSNEHISKVSKMLSDELATAS 56
>UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=2; Giardia intestinalis|Rep: Eukaryotic
peptide chain release factor subunit 1 - Giardia lamblia
(Giardia intestinalis)
Length = 457
Score = 114 bits (275), Expect = 2e-24
Identities = 53/88 (60%), Positives = 66/88 (75%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKSRVN+ +VL AITS RLKLY ++PPNGL +YCG I E+GK KV ID+ P+KPI+
Sbjct: 68 IKSRVNKNAVLSAITSAMSRLKLYNRLPPNGLAVYCGEI-EEDGKNTKVVIDYTPYKPIS 126
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
+YLCD+KFHTE L LL +D+ GFI
Sbjct: 127 NFIYLCDSKFHTEHLRELLTNDDTFGFI 154
Score = 64.1 bits (149), Expect = 4e-09
Identities = 30/58 (51%), Positives = 44/58 (75%), Gaps = 1/58 (1%)
Frame = +1
Query: 352 DRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-ISSHV 522
+R + IW++K+ I+ LE G+GTS+ISL+IPP +Q+S V+ ML +E+GTAS I S V
Sbjct: 15 ERQIAIWQLKRTIEKLESYTGSGTSVISLLIPPGEQLSSVTGMLTNEYGTASNIKSRV 72
>UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative;
n=2; Trichomonas vaginalis|Rep: Eukaryotic release
factor 1, putative - Trichomonas vaginalis G3
Length = 438
Score = 106 bits (255), Expect = 5e-22
Identities = 49/88 (55%), Positives = 63/88 (71%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IK+RVNR SV AIT+ +L + PPNGL ++CGTI+ EEGKEKK+ DFEPF+P++
Sbjct: 64 IKNRVNRQSVEAAITAAIQKLGQFNSTPPNGLCVFCGTII-EEGKEKKLMKDFEPFRPLH 122
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
SLY+CD +FH E L LL + K GFI
Sbjct: 123 QSLYICDRRFHVEPLADLLQTNEKFGFI 150
Score = 58.4 bits (135), Expect = 2e-07
Identities = 25/49 (51%), Positives = 37/49 (75%)
Frame = +1
Query: 361 VEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 507
+E++KIK+LI L A G+GTSM++++IPP D + R+ + L DE GTAS
Sbjct: 14 IELFKIKRLINYLRDAHGDGTSMVTILIPPGDAVHRMRQKLTDELGTAS 62
>UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release
factor 1; n=3; Paramecium tetraurelia|Rep: Eukaryotic
polypeptide chain release factor 1 - Paramecium
tetraurelia
Length = 437
Score = 90.2 bits (214), Expect = 5e-17
Identities = 43/89 (48%), Positives = 59/89 (66%), Gaps = 1/89 (1%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGK-EKKVNIDFEPFKPI 686
IK + NR+SV AI + RL+ Y K P NGLV++CG + +GK EKK+++ EP++P+
Sbjct: 62 IKDKGNRISVQEAIQAAILRLRPYNKAPNNGLVVFCGIVQQADGKGEKKISVVIEPYRPL 121
Query: 687 NTSLYLCDNKFHTEALTALLADDNKLGFI 773
+ SLY CD +FH E L ALL D GFI
Sbjct: 122 DLSLYFCDPQFHVEELRALLNIDPPFGFI 150
Score = 43.6 bits (98), Expect = 0.006
Identities = 21/55 (38%), Positives = 36/55 (65%)
Frame = +1
Query: 343 SSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 507
+ A+ +E +++KKLIK+L R GTS++S+ IPPK IS ++ L ++ A+
Sbjct: 6 NDAEIALEQFRLKKLIKTLSQERTAGTSVVSVYIPPKRIISDITNRLNTQYAEAA 60
>UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=5; Eukaryota|Rep: Eukaryotic peptide chain
release factor subunit 1 - Loxodes striatus
Length = 436
Score = 81.0 bits (191), Expect = 3e-14
Identities = 36/89 (40%), Positives = 59/89 (66%), Gaps = 1/89 (1%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGK-EKKVNIDFEPFKPI 686
IKS+ + +V AI S + +L + ++PPNGL++YCG + E+ K EK++ EP + I
Sbjct: 59 IKSKAVQKAVQDAIISTKQKLSVIKQIPPNGLILYCGKFIGEDEKTEKQILEVLEPLRAI 118
Query: 687 NTSLYLCDNKFHTEALTALLADDNKLGFI 773
NT+ +LC+N F+T+ L +L + +K GFI
Sbjct: 119 NTTFFLCENTFYTQPLRDMLQEQDKFGFI 147
Score = 63.3 bits (147), Expect = 6e-09
Identities = 28/45 (62%), Positives = 37/45 (82%)
Frame = +1
Query: 370 WKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 504
WKI++LI+ LE A+GNGTS+ISLIIPPK+QIS +++ L D G A
Sbjct: 12 WKIRRLIEYLEKAKGNGTSLISLIIPPKEQISLINQKLTDAHGRA 56
>UniRef50_P58227 Cluster: Peptide chain release factor subunit 1;
n=5; Thermoplasmatales|Rep: Peptide chain release factor
subunit 1 - Thermoplasma volcanium
Length = 418
Score = 76.2 bits (179), Expect = 9e-13
Identities = 39/88 (44%), Positives = 51/88 (57%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKS+ R +VL AI S+ RLK Y PPNGLV + G I T + + EP +PI
Sbjct: 60 IKSKSTRKNVLAAIESIMSRLKYYRFPPPNGLVFFVGHIATRGDQTEMYTKIIEPPEPIT 119
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
T +Y CD++FHTE L A+L + G I
Sbjct: 120 TFMYKCDSEFHTEMLKAMLDEKEIYGLI 147
Score = 46.8 bits (106), Expect = 6e-04
Identities = 21/52 (40%), Positives = 31/52 (59%)
Frame = +1
Query: 352 DRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 507
D + ++ K+ ++ L G GT +ISL IPP+ QIS V L DE+ T+S
Sbjct: 7 DEQIRRYEFKRALEELSKLHGRGTELISLYIPPEKQISDVVAYLRDEYSTSS 58
>UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2;
Crenarchaeota|Rep: Peptide chain release factor 1 -
Cenarchaeum symbiosum
Length = 411
Score = 74.9 bits (176), Expect = 2e-12
Identities = 37/92 (40%), Positives = 52/92 (56%), Gaps = 4/92 (4%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTE----EGKEKKVNIDFEPF 677
IKS + R V+ ++ V RL+LY K P GLVI+CG + E G E + +P
Sbjct: 49 IKSDLTRTHVVDSLAKVVQRLRLYKKTPERGLVIFCGALPPEGGGPTGSETVRAFEIDPP 108
Query: 678 KPINTSLYLCDNKFHTEALTALLADDNKLGFI 773
K + T LY CD+ FH + L +L DDN +GF+
Sbjct: 109 KELRTFLYRCDDHFHVDILKGMLKDDNLIGFL 140
Score = 38.3 bits (85), Expect = 0.21
Identities = 17/46 (36%), Positives = 28/46 (60%)
Frame = +1
Query: 367 IWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 504
++KI+K++ L G GT +IS+ IP Q+ + +L +E GTA
Sbjct: 1 MYKIRKMLDELSGISGRGTELISVYIPKGKQLHEIISVLKEEQGTA 46
>UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus
Nitrosopumilus maritimus SCM1|Rep: ERF1 domain 2 protein
- Candidatus Nitrosopumilus maritimus SCM1
Length = 426
Score = 74.5 bits (175), Expect = 3e-12
Identities = 38/92 (41%), Positives = 55/92 (59%), Gaps = 4/92 (4%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEG---KEKKVNI-DFEPF 677
IKS + R V+ ++ V RLKLY K P GLV++CG + EEG + V + + +P
Sbjct: 62 IKSDLTRSHVVDSLGKVVQRLKLYKKTPERGLVMFCGALPPEEGGPLGSEVVKVWEIDPP 121
Query: 678 KPINTSLYLCDNKFHTEALTALLADDNKLGFI 773
K +N LY CD+ FH + L +L DDN +GF+
Sbjct: 122 KDLNQYLYRCDDHFHVDILKDMLKDDNLIGFL 153
Score = 38.3 bits (85), Expect = 0.21
Identities = 17/49 (34%), Positives = 29/49 (59%)
Frame = +1
Query: 358 NVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 504
+V+++KI+K ++ L G GT +I++ IP Q+ + L E GTA
Sbjct: 11 SVKVYKIRKTLEELSKKSGRGTELITVYIPKGKQLHEIIGTLQQEQGTA 59
>UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;
n=5; Archaea|Rep: Peptide chain release factor subunit 1
- Pyrococcus furiosus
Length = 420
Score = 60.9 bits (141), Expect = 3e-08
Identities = 33/88 (37%), Positives = 43/88 (48%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKS+ R +VLGA+ LKLY + P NGL ++ G + EG P +P+N
Sbjct: 59 IKSKTTRKNVLGALERAMQHLKLYKQTPENGLALFVGNVSEMEGVTDIRLWAIIPPEPLN 118
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
LY CD F TE L +L G I
Sbjct: 119 VRLYRCDQTFVTEPLEEMLRVKEAYGLI 146
Score = 45.6 bits (103), Expect = 0.001
Identities = 20/50 (40%), Positives = 33/50 (66%)
Frame = +1
Query: 355 RNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 504
R+ +++++KK I L+ RG GT +ISL IP +S+V + L +E+ TA
Sbjct: 7 RDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREEYSTA 56
>UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;
n=7; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Halobacterium salinarium (Halobacterium
halobium)
Length = 416
Score = 60.9 bits (141), Expect = 3e-08
Identities = 34/90 (37%), Positives = 50/90 (55%), Gaps = 2/90 (2%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPP-NGLVIYCGTIVTEEGKEKKVNIDFE-PFKP 683
IKS+ R +V A+TS++ RL+ Y PP NG+V++ G + + G+ V E P +P
Sbjct: 62 IKSKQTRTNVQDALTSIKDRLRYYDTFPPDNGMVVFSGAVDSGGGRTDMVTEVLESPPQP 121
Query: 684 INTSLYLCDNKFHTEALTALLADDNKLGFI 773
I + Y CD+ F TE L +L D G I
Sbjct: 122 IESFRYHCDSAFLTEPLAEMLGDKGLYGLI 151
Score = 37.1 bits (82), Expect = 0.49
Identities = 20/61 (32%), Positives = 34/61 (55%)
Frame = +1
Query: 325 NKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 504
++ E S DR ++ +K+I+ L+ G+GT ++++ IPP QIS V + E A
Sbjct: 2 SEQDEVPSEDRRK--YEFRKVIEELKDYEGSGTQLVTIYIPPDKQISDVVAHVTQEHSEA 59
Query: 505 S 507
S
Sbjct: 60 S 60
>UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;
n=12; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Methanococcus jannaschii
Length = 421
Score = 58.0 bits (134), Expect = 2e-07
Identities = 33/89 (37%), Positives = 47/89 (52%), Gaps = 1/89 (1%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTE-EGKEKKVNIDFEPFKPI 686
IKS+ R +V AI ++ RLKL + G+VI+ G + G EK EP +PI
Sbjct: 57 IKSKSTRKNVQSAIEAILQRLKLLKEPLEKGVVIFAGMVPRSGPGTEKMETYVIEPPEPI 116
Query: 687 NTSLYLCDNKFHTEALTALLADDNKLGFI 773
T +Y CD++F+ E L L D + G I
Sbjct: 117 KTYIYRCDSEFYLEPLEEFLEDKDAYGVI 145
Score = 41.1 bits (92), Expect = 0.030
Identities = 19/48 (39%), Positives = 31/48 (64%)
Frame = +1
Query: 364 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 507
+++ KK++K L+ +G GT +ISL IP +IS V++ L +E AS
Sbjct: 8 QLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQAS 55
>UniRef50_Q9YAF1 Cluster: Peptide chain release factor subunit 1;
n=9; Thermoprotei|Rep: Peptide chain release factor
subunit 1 - Aeropyrum pernix
Length = 373
Score = 55.6 bits (128), Expect = 1e-06
Identities = 31/88 (35%), Positives = 50/88 (56%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IK + R +V A+++ RL++ T PPNGLV++CG ++ GK + F P +PI
Sbjct: 62 IKLKRTRQAVKRALSAAMDRLQMLTSTPPNGLVLFCGEDMS-TGKFE--CFMFSPPEPIR 118
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
Y D +F T+ L ++ D+N +G I
Sbjct: 119 VFYYRTDKRFITDFLEDMVEDNNAIGII 146
>UniRef50_Q46FF0 Cluster: Peptide chain release factor, subunit 1;
n=2; Methanosarcinaceae|Rep: Peptide chain release
factor, subunit 1 - Methanosarcina barkeri (strain
Fusaro / DSM 804)
Length = 415
Score = 55.2 bits (127), Expect = 2e-06
Identities = 29/88 (32%), Positives = 48/88 (54%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IKS++ +V GA+ S+ +L+ K+P NG+V + G I T + V P +P+
Sbjct: 57 IKSKLTSNNVQGALDSLLAKLRSLNKIPENGIVYFTGVIDTGANRTGMVTEVLIPPEPVV 116
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
+Y CD+ F+ E L +L + + GFI
Sbjct: 117 DYIYHCDSVFYLEPLEEMLRECSTYGFI 144
Score = 34.3 bits (75), Expect = 3.4
Identities = 19/48 (39%), Positives = 26/48 (54%)
Frame = +1
Query: 364 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 507
E + KK +++L G T +ISL IP QIS V+ L +E AS
Sbjct: 8 EKYVFKKKLETLRNKNGKSTELISLYIPSDKQISDVTNHLREEHEQAS 55
>UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:
NEQ052 - Nanoarchaeum equitans
Length = 358
Score = 53.6 bits (123), Expect = 5e-06
Identities = 29/88 (32%), Positives = 45/88 (51%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IK R +V A+ + + LK + K+P NGLVI+ G I ++ ++ P K N
Sbjct: 54 IKDDTTRKNVQAALAKIINYLKDFKKIPENGLVIFAGNIAGRGDQDVRLWAIVPPEKS-N 112
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
LY CD+KF+ E L +L + G +
Sbjct: 113 LKLYRCDSKFYVEPLEEMLEEKKLYGIV 140
Score = 38.3 bits (85), Expect = 0.21
Identities = 17/48 (35%), Positives = 28/48 (58%)
Frame = +1
Query: 364 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 507
E +K+K L++ L+ G GT ++SL IP I ++ L E+ TA+
Sbjct: 5 EKYKLKSLLRQLKSIEGRGTELVSLYIPSNYPIGKILDQLNYEYATAA 52
>UniRef50_Q8ZU81 Cluster: Peptide chain release factor subunit 1;
n=5; Thermoproteaceae|Rep: Peptide chain release factor
subunit 1 - Pyrobaculum aerophilum
Length = 388
Score = 52.8 bits (121), Expect = 9e-06
Identities = 26/75 (34%), Positives = 38/75 (50%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
IK + R V + + + LK K P NG+ ++ G + +G + V P +PIN
Sbjct: 64 IKDKTTRTHVQDTLERIINNLKGEAKAPENGMAVFAGFHMINQGNYEWVYYVVIPPQPIN 123
Query: 690 TSLYLCDNKFHTEAL 734
T Y+CD FHTE L
Sbjct: 124 TFKYICDTAFHTELL 138
>UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-2;
n=9; Euryarchaeota|Rep: Peptide chain release factor
subunit 1-2 - Methanosarcina acetivorans
Length = 415
Score = 50.0 bits (114), Expect = 6e-05
Identities = 27/88 (30%), Positives = 47/88 (53%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
I S++ R +V A+ S+ +L+ K+P NG+V + GT+ T + VN P +P+
Sbjct: 57 IISKLIRNNVQEALHSLLAKLRSLHKIPENGIVYFTGTVDTGANRTGMVNEVLFPPEPVA 116
Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
+Y CD+ F+ E L +L + G +
Sbjct: 117 DYIYRCDSVFYLEPLEEMLRECTTYGLL 144
Score = 40.3 bits (90), Expect = 0.052
Identities = 21/48 (43%), Positives = 29/48 (60%)
Frame = +1
Query: 364 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 507
E + KK +++L+ G T +ISL IPP QIS V+K L +E AS
Sbjct: 8 EKYVFKKQLETLKSKNGRSTELISLYIPPDKQISDVTKHLREEHEQAS 55
>UniRef50_UPI000150A1F7 Cluster: eRF1 domain 1 family protein; n=1;
Tetrahymena thermophila SB210|Rep: eRF1 domain 1 family
protein - Tetrahymena thermophila SB210
Length = 519
Score = 42.7 bits (96), Expect = 0.010
Identities = 26/90 (28%), Positives = 46/90 (51%), Gaps = 2/90 (2%)
Frame = +3
Query: 510 IKSRVNRLSVLGAITSVQHRLKLYT--KVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKP 683
IK+ NR SV A++ ++ RLK + K P G++ +C + + K + I +P K
Sbjct: 125 IKNTQNRKSVQSALSMLRERLKNFNLHKKYPKGMIFFCADSL--DSKRLLIEI-LDPPKA 181
Query: 684 INTSLYLCDNKFHTEALTALLADDNKLGFI 773
+ + Y C+ F+ + L +L D GF+
Sbjct: 182 VQSFRYSCNTIFYLDDLEYMLKDQPTYGFV 211
>UniRef50_Q3UUT4 Cluster: 16 days neonate thymus cDNA, RIKEN
full-length enriched library, clone:A130075O06
product:hypothetical protein, full insert sequence; n=1;
Mus musculus|Rep: 16 days neonate thymus cDNA, RIKEN
full-length enriched library, clone:A130075O06
product:hypothetical protein, full insert sequence - Mus
musculus (Mouse)
Length = 103
Score = 34.7 bits (76), Expect = 2.6
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = +3
Query: 645 EKKVNIDFEPFKPINTSLYLCD 710
+KK +DFEPFKP++TSL + D
Sbjct: 28 KKKARVDFEPFKPMSTSLRVAD 49
>UniRef50_Q97DK8 Cluster: Uncharacterized conserved protein, phnB
family; n=3; Bacteria|Rep: Uncharacterized conserved
protein, phnB family - Clostridium acetobutylicum
Length = 294
Score = 34.7 bits (76), Expect = 2.6
Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 1/62 (1%)
Frame = +3
Query: 543 GAITS-VQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKF 719
G++T + ++ YT + N + + E K K I+F FK NT+ DN F
Sbjct: 142 GSVTGKAEEAMEFYTSIFENSEITFINRYAEGEAKAKNAKINFASFKLNNTNFSASDNGF 201
Query: 720 HT 725
T
Sbjct: 202 DT 203
>UniRef50_A2DQW1 Cluster: DHHC zinc finger domain containing
protein; n=1; Trichomonas vaginalis G3|Rep: DHHC zinc
finger domain containing protein - Trichomonas vaginalis
G3
Length = 347
Score = 33.5 bits (73), Expect = 6.0
Identities = 19/55 (34%), Positives = 27/55 (49%)
Frame = -1
Query: 378 YLPYFDISIGRRFFRHFVSLFI*LINHKHYICINN*NNVFAHTKTLNKRILWKGS 214
Y P+ + IG R R+F+ I I Y CIN+ VF + + +RI W S
Sbjct: 172 YCPWVLVPIGERNHRYFLLFLIACIVASTYYCINDVFVVFFYFMQVYQRIPWTNS 226
>UniRef50_UPI00006CC094 Cluster: hypothetical protein
TTHERM_00215930; n=1; Tetrahymena thermophila SB210|Rep:
hypothetical protein TTHERM_00215930 - Tetrahymena
thermophila SB210
Length = 905
Score = 33.1 bits (72), Expect = 7.9
Identities = 17/41 (41%), Positives = 26/41 (63%)
Frame = +1
Query: 373 KIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEF 495
K++ LI+ L NGT+ +SL I K Q +++S+ L DEF
Sbjct: 182 KVEYLIEQLLEGSNNGTNTLSLKILQKQQANQLSQDLKDEF 222
>UniRef50_Q8I368 Cluster: Putative uncharacterized protein PFI0400c;
n=2; Plasmodium|Rep: Putative uncharacterized protein
PFI0400c - Plasmodium falciparum (isolate 3D7)
Length = 352
Score = 33.1 bits (72), Expect = 7.9
Identities = 18/58 (31%), Positives = 29/58 (50%)
Frame = -1
Query: 459 LILRWNDQGYHRCSISSGHFQTLYKFLYLPYFDISIGRRFFRHFVSLFI*LINHKHYI 286
+IL W+ Y +SS H QTLY FL + + I++ +FV + I + + I
Sbjct: 172 IILFWSQVYYASVLVSSPHLQTLYIFLNVLVYAINVILASLAYFVKAYFDYIYYNYII 229
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 752,677,981
Number of Sequences: 1657284
Number of extensions: 14691191
Number of successful extensions: 36551
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 35276
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36535
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 65027411410
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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