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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0416.Seq
         (774 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g47880.1 68418.m05915 eukaryotic peptide chain release factor...   150   9e-37
At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot...   149   2e-36
At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot...   143   1e-34
At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ...    29   2.6  
At4g14480.1 68417.m02233 protein kinase family protein contains ...    29   4.5  
At5g56240.1 68418.m07018 expressed protein                             28   7.9  
At3g59610.1 68416.m06651 F-box family protein / jacalin lectin f...    28   7.9  

>At5g47880.1 68418.m05915 eukaryotic peptide chain release factor
           subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic
           peptide chain release factor subunit 1-1 (eRF1-1)
           (Eukaryotic release factor 1-1) (Omnipotent suppressor
           protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis
           thaliana}, eukaryotic release factor 1 homolog
           GI:1155261 from [Arabidopsis thaliana]; contains Pfam
           profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2,
           PF03465 eRF1 domain 3
          Length = 436

 Score =  150 bits (364), Expect = 9e-37
 Identities = 70/88 (79%), Positives = 75/88 (85%)
 Frame = +3

Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
           IKSRVNR SVLGAITS Q RLKLY +VPPNGLV+Y GTIV E+GKEKKV IDFEPF+PIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120

Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
            SLYLCDNKFHTEAL  LL  D+K GFI
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFI 148



 Score =   99 bits (238), Expect = 2e-21
 Identities = 47/65 (72%), Positives = 57/65 (87%), Gaps = 1/65 (1%)
 Frame = +1

Query: 331 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS- 507
           M +++  D+N+EIWKIKKLIKSLE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTAS 
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 508 ISSHV 522
           I S V
Sbjct: 61  IKSRV 65


>At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein
           / eRF1 family protein contains Pfam profiles: PF03463
           eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
           domain 3
          Length = 435

 Score =  149 bits (361), Expect = 2e-36
 Identities = 70/88 (79%), Positives = 74/88 (84%)
 Frame = +3

Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
           IKSRVNR SVL AITS Q RLKLY KVPPNGLV+Y GTIVT++GKEKKV IDFEPFKPIN
Sbjct: 60  IKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPIN 119

Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
            SLYLCDNKFHTE L  LL  D+K GFI
Sbjct: 120 ASLYLCDNKFHTEPLNELLESDDKFGFI 147



 Score = 97.1 bits (231), Expect = 1e-20
 Identities = 45/62 (72%), Positives = 55/62 (88%), Gaps = 1/62 (1%)
 Frame = +1

Query: 340 ESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-ISS 516
           +  +D+N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ++RV+KMLADE+GTAS I S
Sbjct: 3   DQESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASNIKS 62

Query: 517 HV 522
            V
Sbjct: 63  RV 64


>At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein
           / eRF1 family protein contains Pfam profiles: PF03463
           eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
           domain 3
          Length = 434

 Score =  143 bits (346), Expect = 1e-34
 Identities = 67/88 (76%), Positives = 72/88 (81%)
 Frame = +3

Query: 510 IKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPIN 689
           IKSRVNR SVL AITS Q RLKLY KVP NGLV+Y GTIV ++GKEKKV  DFEPF+PIN
Sbjct: 59  IKSRVNRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPIN 118

Query: 690 TSLYLCDNKFHTEALTALLADDNKLGFI 773
            SLYLCDNKFHTEAL  LL  D+K GFI
Sbjct: 119 ASLYLCDNKFHTEALNELLESDDKFGFI 146



 Score = 97.1 bits (231), Expect = 1e-20
 Identities = 49/65 (75%), Positives = 56/65 (86%), Gaps = 1/65 (1%)
 Frame = +1

Query: 331 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS- 507
           M+EE  AD N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTAS 
Sbjct: 1   MAEE--ADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 58

Query: 508 ISSHV 522
           I S V
Sbjct: 59  IKSRV 63


>At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains
           similarity to NAD kinase [Homo sapiens]
           gi|20070086|gb|AAM01195; contains Pfam domain, PF01513:
           ATP-NAD kinase
          Length = 985

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = +1

Query: 391 KSLEMARGNGTSMISLIIPPKDQISR 468
           KS+   RGNG S   + +PP D +SR
Sbjct: 561 KSISDNRGNGFSAAPIAVPPSDNLSR 586


>At4g14480.1 68417.m02233 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 487

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 16/54 (29%), Positives = 30/54 (55%)
 Frame = +1

Query: 322 RNKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKML 483
           +N+  E    D+++ + K+K L KSLE  R   + +I  +   K++ SR  ++L
Sbjct: 407 KNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELL 460


>At5g56240.1 68418.m07018 expressed protein 
          Length = 986

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = +3

Query: 342 IVGRSKCRNMEDKETYKEFGNGQRKWNIDDIPDHST*GSNLESIKDVG 485
           I G++ C+  ED+    E G G     +D IP     GS L S +D+G
Sbjct: 668 IYGQTSCK--EDERFDSEVGQGSLFVEVDTIPIPGPPGSFLPSPRDMG 713


>At3g59610.1 68416.m06651 F-box family protein / jacalin lectin
           family protein contains Pfam profile PF01419:
           Jacalin-like lectin domain
          Length = 521

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 12/45 (26%), Positives = 25/45 (55%)
 Frame = +1

Query: 331 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQIS 465
           + +E    RN+EIW  K+ IK+ +       +++S+++P    +S
Sbjct: 251 LEQEIYNTRNIEIWVTKETIKNGDGEAVEWVNLMSVLVPEWSSLS 295


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,676,028
Number of Sequences: 28952
Number of extensions: 341268
Number of successful extensions: 908
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 873
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 908
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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