BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0408.Seq (735 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g35890.1 68417.m05097 La domain-containing protein contains P... 32 0.34 At2g41300.1 68415.m05100 strictosidine synthase family protein s... 30 1.4 At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family... 30 1.8 At3g07780.1 68416.m00949 expressed protein 29 4.2 At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 28 5.6 At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 28 5.6 At5g13800.2 68418.m01610 hydrolase, alpha/beta fold family prote... 28 7.4 At5g13800.1 68418.m01609 hydrolase, alpha/beta fold family prote... 28 7.4 At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 28 7.4 At5g52280.1 68418.m06488 protein transport protein-related low s... 27 9.8 >At4g35890.1 68417.m05097 La domain-containing protein contains Pfam PF05383: La domain Length = 523 Score = 32.3 bits (70), Expect = 0.34 Identities = 19/57 (33%), Positives = 24/57 (42%) Frame = +1 Query: 172 PAPKQEGEGDPEFIKRQDQKRSDLDEQLRNTSTNGANSGPRRRMSSNALKRSRPSAR 342 PAPKQ G +P ++RS + S NG S P + S L PS R Sbjct: 182 PAPKQAGRANPNPTPNHSRQRSFKQRNGASGSANGTVSQPSAQGSFTELPSHNPSPR 238 >At2g41300.1 68415.m05100 strictosidine synthase family protein similar to strictosidine synthase [Rauvolfia serpentina][SP|P15324]; contains strictosidine synthase domain PF03088; protein alignments support a CG non-consensus donor splice site. Length = 394 Score = 30.3 bits (65), Expect = 1.4 Identities = 16/33 (48%), Positives = 18/33 (54%), Gaps = 1/33 (3%) Frame = -2 Query: 707 PRVPGPSVGVCRRRDPRWSAA*CGWTE-RFSSP 612 PR GP VGV R +WS GW E +SSP Sbjct: 86 PRGEGPYVGVTDGRILKWSGEDLGWIEFAYSSP 118 >At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family protein Length = 635 Score = 29.9 bits (64), Expect = 1.8 Identities = 15/45 (33%), Positives = 25/45 (55%) Frame = +2 Query: 482 PGYAPGHERASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 616 PGYAP HE + +G +F K + +S ++ +K+ E+L E Sbjct: 475 PGYAPTHEGDNSSGWSFPTSKGNIYISISASKPYLHKSHEELRWE 519 >At3g07780.1 68416.m00949 expressed protein Length = 566 Score = 28.7 bits (61), Expect = 4.2 Identities = 20/59 (33%), Positives = 35/59 (59%), Gaps = 2/59 (3%) Frame = +2 Query: 563 LSNAQLERNKTKEQLEEEKKISLSIRIKPLTIE--GLSVDKLRQKAQELWECIVKLETE 733 ++ Q+ER K K+Q+EE ++I +R+K E L ++ + +A+ L E IVK + E Sbjct: 434 VAELQMERQKKKQQIEEVERI---VRLKQAEAEMFQLKANEAKVEAERL-ERIVKAKKE 488 >At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation protein, putative nearly identical to SHEPHERD [Arabidopsis thaliana] GI:19570872; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 823 Score = 28.3 bits (60), Expect = 5.6 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = -3 Query: 724 EFDDALPEFLGLLSEFVDGETL 659 EFD+ LP++L L VD +TL Sbjct: 428 EFDELLPKYLSFLKGLVDSDTL 449 >At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation protein, putative nearly identical to SHEPHERD [Arabidopsis thaliana] GI:19570872; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 823 Score = 28.3 bits (60), Expect = 5.6 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = -3 Query: 724 EFDDALPEFLGLLSEFVDGETL 659 EFD+ LP++L L VD +TL Sbjct: 428 EFDELLPKYLSFLKGLVDSDTL 449 >At5g13800.2 68418.m01610 hydrolase, alpha/beta fold family protein low similarity to hydrolase [Terrabacter sp. DBF63] GI:14196240; contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 484 Score = 27.9 bits (59), Expect = 7.4 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = -2 Query: 635 WTERFSSPLPAAPWSCCAPAGHC 567 W ++ +P AP+ +PAGHC Sbjct: 372 WGKKIKKEIPNAPYYEISPAGHC 394 >At5g13800.1 68418.m01609 hydrolase, alpha/beta fold family protein low similarity to hydrolase [Terrabacter sp. DBF63] GI:14196240; contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 484 Score = 27.9 bits (59), Expect = 7.4 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = -2 Query: 635 WTERFSSPLPAAPWSCCAPAGHC 567 W ++ +P AP+ +PAGHC Sbjct: 372 WGKKIKKEIPNAPYYEISPAGHC 394 >At1g21630.1 68414.m02708 calcium-binding EF hand family protein contains INTERPRO:IPR002048 calcium-binding EF-hand domain; ESTs gb|T44428 and gb|AA395440 come from this gene Length = 1218 Score = 27.9 bits (59), Expect = 7.4 Identities = 17/49 (34%), Positives = 28/49 (57%) Frame = +2 Query: 581 ERNKTKEQLEEEKKISLSIRIKPLTIEGLSVDKLRQKAQELWECIVKLE 727 E ++ EE+ K S ++ K LTIE + +++K EL++ IVK E Sbjct: 656 ELESLAKKYEEKYKKSGNVGSK-LTIEEATFRDIQEKKMELYQAIVKFE 703 >At5g52280.1 68418.m06488 protein transport protein-related low similarity to SP|P25386 Intracellular protein transport protein USO1 {Saccharomyces cerevisiae} Length = 853 Score = 27.5 bits (58), Expect = 9.8 Identities = 15/56 (26%), Positives = 33/56 (58%) Frame = +2 Query: 527 NFTIQKKSENFGLSNAQLERNKTKEQLEEEKKISLSIRIKPLTIEGLSVDKLRQKA 694 N T+++ E ++ ++ + K + + EEK +LS++++ L E L + KLR ++ Sbjct: 619 NKTLEEMQEK---THTEITQEKEQRKHVEEKNKALSMKVQMLESEVLKLTKLRDES 671 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,592,259 Number of Sequences: 28952 Number of extensions: 177724 Number of successful extensions: 812 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 775 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 811 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1614253080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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