Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0406.Seq
(650 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g18640.2 68414.m02324 3-phosphoserine phosphatase (PSP) nearl... 101 5e-22
At1g18640.1 68414.m02323 3-phosphoserine phosphatase (PSP) nearl... 101 5e-22
At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative ... 36 0.023
At5g30510.1 68418.m03752 30S ribosomal protein S1, putative simi... 30 1.5
At5g24160.1 68418.m02842 squalene monooxygenase 1,2 / squalene e... 30 1.5
At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 fac... 30 1.5
At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 fac... 30 1.5
At4g11110.1 68417.m01803 WD-40 repeat family protein / phytochro... 29 2.7
At5g55820.1 68418.m06956 expressed protein 29 3.5
At5g24150.1 68418.m02839 squalene monooxygenase 1,1 / squalene e... 28 4.7
At5g61480.1 68418.m07714 leucine-rich repeat transmembrane prote... 28 6.2
At1g21590.1 68414.m02699 protein kinase family protein contains ... 28 6.2
At5g60350.1 68418.m07566 hypothetical protein 27 8.2
At4g21600.1 68417.m03128 bifunctional nuclease, putative similar... 27 8.2
At2g30340.1 68415.m03692 LOB domain protein 13 / lateral organ b... 27 8.2
>At1g18640.2 68414.m02324 3-phosphoserine phosphatase (PSP) nearly
identical to 3-phosphoserine phosphatase GI:3759177 from
[Arabidopsis thaliana]
Length = 295
Score = 101 bits (241), Expect = 5e-22
Identities = 46/89 (51%), Positives = 64/89 (71%)
Frame = +3
Query: 255 AEAMGGNMTFQEALKKRLDIIRPNVGQIREFIEKFPVRLTPGITELVKELHERGVIVYLV 434
A AMGG++ F+EAL RL + +P++ ++ E+++K P RL+PGI ELVK+L + VYL+
Sbjct: 118 ARAMGGSVPFEEALAARLSLFKPSLSKVEEYLDKRPPRLSPGIEELVKKLRANNIDVYLI 177
Query: 435 SGGFRSLIEPVAERLNIPTINIFANDSSF 521
SGGFR +I PVA L IP NIFAN+ F
Sbjct: 178 SGGFRQMINPVASILGIPRENIFANNLLF 206
Score = 60.5 bits (140), Expect = 9e-10
Identities = 26/48 (54%), Positives = 34/48 (70%)
Frame = +1
Query: 118 PQQTVQELFRTADCVCFDVDSTVIQDEGIDELAKFCGKGDEVKRLTRK 261
P + + +L+R+ + VCFDVDSTV DEGIDELA+FCG G V T +
Sbjct: 72 PSKEILDLWRSVEAVCFDVDSTVCVDEGIDELAEFCGAGKAVAEWTAR 119
Score = 54.0 bits (124), Expect = 8e-08
Identities = 23/46 (50%), Positives = 34/46 (73%)
Frame = +2
Query: 512 LKFYFNGEYAGFDENEPTSRSGGKGLVVRRLKELHGYQRLVIVGDG 649
L F +GE+ GFDENEPTSRSGGK V+++++ Y+ + ++GDG
Sbjct: 204 LLFGNSGEFLGFDENEPTSRSGGKAKAVQQIRKGRLYKTMAMIGDG 249
>At1g18640.1 68414.m02323 3-phosphoserine phosphatase (PSP) nearly
identical to 3-phosphoserine phosphatase GI:3759177 from
[Arabidopsis thaliana]
Length = 295
Score = 101 bits (241), Expect = 5e-22
Identities = 46/89 (51%), Positives = 64/89 (71%)
Frame = +3
Query: 255 AEAMGGNMTFQEALKKRLDIIRPNVGQIREFIEKFPVRLTPGITELVKELHERGVIVYLV 434
A AMGG++ F+EAL RL + +P++ ++ E+++K P RL+PGI ELVK+L + VYL+
Sbjct: 118 ARAMGGSVPFEEALAARLSLFKPSLSKVEEYLDKRPPRLSPGIEELVKKLRANNIDVYLI 177
Query: 435 SGGFRSLIEPVAERLNIPTINIFANDSSF 521
SGGFR +I PVA L IP NIFAN+ F
Sbjct: 178 SGGFRQMINPVASILGIPRENIFANNLLF 206
Score = 60.5 bits (140), Expect = 9e-10
Identities = 26/48 (54%), Positives = 34/48 (70%)
Frame = +1
Query: 118 PQQTVQELFRTADCVCFDVDSTVIQDEGIDELAKFCGKGDEVKRLTRK 261
P + + +L+R+ + VCFDVDSTV DEGIDELA+FCG G V T +
Sbjct: 72 PSKEILDLWRSVEAVCFDVDSTVCVDEGIDELAEFCGAGKAVAEWTAR 119
Score = 54.0 bits (124), Expect = 8e-08
Identities = 23/46 (50%), Positives = 34/46 (73%)
Frame = +2
Query: 512 LKFYFNGEYAGFDENEPTSRSGGKGLVVRRLKELHGYQRLVIVGDG 649
L F +GE+ GFDENEPTSRSGGK V+++++ Y+ + ++GDG
Sbjct: 204 LLFGNSGEFLGFDENEPTSRSGGKAKAVQQIRKGRLYKTMAMIGDG 249
>At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative
strong similarity to hydroxymethylpyrimidine
kinase/thiamin-phosphate pyrophosphorylase (BTH1)
GI:7488455 from [Brassica napus]
Length = 522
Score = 35.9 bits (79), Expect = 0.023
Identities = 19/54 (35%), Positives = 25/54 (46%)
Frame = +3
Query: 282 FQEALKKRLDIIRPNVGQIREFIEKFPVRLTPGITELVKELHERGVIVYLVSGG 443
F+E L DII PNV + ++ F + + K LHE G LV GG
Sbjct: 152 FRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGG 205
>At5g30510.1 68418.m03752 30S ribosomal protein S1, putative similar
to Swiss-Prot:P29344 30S ribosomal protein S1,
chloroplast precursor (CS1) [Spinacia oleracea]
Length = 416
Score = 29.9 bits (64), Expect = 1.5
Identities = 19/52 (36%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
Frame = -1
Query: 305 SLLQRF--LECHITAHCFRVSLLTSSPFPQNLASSSMPSSCMTVESTSKQTQ 156
SL Q+F L C + R+S S FPQN ++S P+ V +S QT+
Sbjct: 3 SLAQQFSGLRCSPLSSSSRLSRRASKNFPQNKSASVSPTIVAAVAMSSGQTK 54
>At5g24160.1 68418.m02842 squalene monooxygenase 1,2 / squalene
epoxidase 1,2 (SQP1,2) identical to SP|O65402
Length = 517
Score = 29.9 bits (64), Expect = 1.5
Identities = 20/63 (31%), Positives = 27/63 (42%)
Frame = +3
Query: 264 MGGNMTFQEALKKRLDIIRPNVGQIREFIEKFPVRLTPGITELVKELHERGVIVYLVSGG 443
+G E + DI +P + F L G T+ KE +GV YL SGG
Sbjct: 369 LGDANKVSEVINSFYDIRKPMSATVNTLGNAFSQVLI-GSTDEAKEAMRQGVYDYLCSGG 427
Query: 444 FRS 452
FR+
Sbjct: 428 FRT 430
>At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 575
Score = 29.9 bits (64), Expect = 1.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 383 YRVSERVTRERSHRISSFGRIQKSNRT 463
YR ER++RE + R+ GRI + + T
Sbjct: 289 YREKERISREEAWRVQEIGRINREHET 315
>At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 498
Score = 29.9 bits (64), Expect = 1.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 383 YRVSERVTRERSHRISSFGRIQKSNRT 463
YR ER++RE + R+ GRI + + T
Sbjct: 212 YREKERISREEAWRVQEIGRINREHET 238
>At4g11110.1 68417.m01803 WD-40 repeat family protein / phytochrome
A-related contains 7 WD-40 repeats (PF00400); similar to
phytochrome A supressor spa1 (GI:4809171) [Arabidopsis
thaliana]; contains non-consensus (GC) donor splice
sites at introns 4 and 6
Length = 1017
Score = 29.1 bits (62), Expect = 2.7
Identities = 18/58 (31%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
Frame = +3
Query: 342 EFIEKFPVRLT-PGITELVKELHERGVIVYLVSGGFRSLIEPVAERLNIPTINIFAND 512
+F+ +FP+ PG + + K++ +RG + L + G SL E +L I +N AN+
Sbjct: 124 QFLHRFPLDGDLPGSSSMSKKVIDRGTVSILRNAGKMSLPETSNGQLAIIAVNGEANE 181
>At5g55820.1 68418.m06956 expressed protein
Length = 1826
Score = 28.7 bits (61), Expect = 3.5
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -2
Query: 487 GMFSLSATGSIRLLNPPETRYTMTPLSCNSFTNSVIP 377
G + G+ R + PP +R++ LSCNS +V P
Sbjct: 1442 GKENQGGAGAKRNVKPPSSRFSKPKLSCNSSLTTVGP 1478
>At5g24150.1 68418.m02839 squalene monooxygenase 1,1 / squalene
epoxidase 1,1 (SQP1,1) identical to SP|O65404
Length = 516
Score = 28.3 bits (60), Expect = 4.7
Identities = 18/63 (28%), Positives = 26/63 (41%)
Frame = +3
Query: 264 MGGNMTFQEALKKRLDIIRPNVGQIREFIEKFPVRLTPGITELVKELHERGVIVYLVSGG 443
+G + +K DI +P + F L T+ KE +G YL SGG
Sbjct: 368 LGNAQKISQVIKSFYDIRKPMSATVNTLGNAFSQVLVAS-TDEAKEAMRQGCYDYLSSGG 426
Query: 444 FRS 452
FR+
Sbjct: 427 FRT 429
>At5g61480.1 68418.m07714 leucine-rich repeat transmembrane protein
kinase, putative
Length = 1041
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/54 (31%), Positives = 27/54 (50%)
Frame = -2
Query: 529 IEIKLESLAKMLIVGMFSLSATGSIRLLNPPETRYTMTPLSCNSFTNSVIPGVS 368
I I++ L+ +L + + S GS T+ T +S NSF +S PG+S
Sbjct: 97 IPIQIRYLSSLLYLNLSGNSLEGSFPTSIFDLTKLTTLDISRNSFDSSFPPGIS 150
>At1g21590.1 68414.m02699 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 756
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/19 (73%), Positives = 14/19 (73%)
Frame = +3
Query: 354 KFPVRLTPGITELVKELHE 410
KFP LTP IT LVKEL E
Sbjct: 265 KFPEDLTPFITMLVKELPE 283
>At5g60350.1 68418.m07566 hypothetical protein
Length = 292
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/29 (44%), Positives = 20/29 (68%), Gaps = 2/29 (6%)
Frame = +2
Query: 395 ERVTRE--RSHRISSFGRIQKSNRTGRGE 475
E++T+E R+ + SFG I +S RTG+ E
Sbjct: 2 EKITKEERRNRCLPSFGSIYRSKRTGKKE 30
>At4g21600.1 68417.m03128 bifunctional nuclease, putative similar to
bifunctional nuclease [Zinnia elegans]
gi|4099833|gb|AAD00694
Length = 296
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 211 LAKFCGKGDEVKRLTRKQWAVI*H 282
LA FC DE+K+L++ QW H
Sbjct: 58 LADFCSWPDEIKKLSQWQWTSTLH 81
>At2g30340.1 68415.m03692 LOB domain protein 13 / lateral organ
boundaries domain protein 13 (LBD13) identical to LOB
DOMAIN 13 [Arabidopsis thaliana] GI:17227158 SP|Q9AT61
Length = 268
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -1
Query: 248 LLTSSPFPQNLASSSMPSSCMTVESTSKQTQSA 150
LL+S P P S+PS M V S+S SA
Sbjct: 207 LLSSQPAPPPTPPVSLPSPSMVVSSSSSSNSSA 239
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,322,950
Number of Sequences: 28952
Number of extensions: 272376
Number of successful extensions: 872
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 847
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 872
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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