BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0400X.Seq (492 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subun... 59 3e-10 SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy... 30 0.16 SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S... 28 0.66 SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr... 26 2.7 SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 26 3.5 SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 25 8.2 >SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subunit Cgs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 59.3 bits (137), Expect = 3e-10 Identities = 38/91 (41%), Positives = 57/91 (62%), Gaps = 11/91 (12%) Frame = +3 Query: 252 GDDGDNFYVIENGVFDVLVTGDDRV--EKVVHTYEGS-------GS-FGELALMYNMPLA 401 G GD FY++E G FDV + + E+V+ + G+ G FGELALMYN P A Sbjct: 175 GAVGDYFYIVEQGEFDVYKRPELNITPEEVLSSGYGNYITTISPGEYFGELALMYNAPRA 234 Query: 402 ASVRSQTA-GALWAMDRHTFRRILLKSAFKK 491 ASV S+T ++A+DR +FRRI+ ++A+++ Sbjct: 235 ASVVSKTPNNVIYALDRTSFRRIVFENAYRQ 265 Score = 45.6 bits (103), Expect = 4e-06 Identities = 28/73 (38%), Positives = 42/73 (57%) Frame = +3 Query: 252 GDDGDNFYVIENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMYNMPLAASVRSQTAGA 431 GD G+ FY+IE+G +V+ G + VV T FGELAL++ A+V+++T Sbjct: 309 GDIGNQFYLIEDGEAEVVKNG----KGVVVTLTKGDYFGELALIHETVRNATVQAKTRLK 364 Query: 432 LWAMDRHTFRRIL 470 L D+ TF R+L Sbjct: 365 LATFDKPTFNRLL 377 Score = 25.0 bits (52), Expect = 6.2 Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%) Frame = +2 Query: 32 RRKSVFAETYDPEEDDSDEGAPAVFPKSDAQRA-RLAEAVRGILLFRSLD 178 RR+SV E+ +P + FP D + RL +V G LF++LD Sbjct: 101 RRQSVSTESMNPSAFALE--TKRTFPPKDPEDLKRLKRSVAGNFLFKNLD 148 >SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces pombe|chr 1|||Manual Length = 348 Score = 30.3 bits (65), Expect = 0.16 Identities = 13/40 (32%), Positives = 24/40 (60%) Frame = +3 Query: 324 VEKVVHTYEGSGSFGELALMYNMPLAASVRSQTAGALWAM 443 ++ +HTY+G S + L+ ++PL++ S TA L +M Sbjct: 267 IDSFIHTYDGFSSMTQCLLIISLPLSSLWASSTALKLQSM 306 >SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1206 Score = 28.3 bits (60), Expect = 0.66 Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = +3 Query: 279 IENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMY--NMPLAASVRSQTAGALW 437 ++ F +L TGD + K+ ++G G+ EL L Y +PLA + G L+ Sbjct: 313 MKGSFFYLLQTGDGDLLKLTIEHDGQGNVVELRLKYFDTVPLAVQLNILKTGFLF 367 >SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 872 Score = 26.2 bits (55), Expect = 2.7 Identities = 29/123 (23%), Positives = 53/123 (43%), Gaps = 3/123 (2%) Frame = -3 Query: 475 LSRMRRNVWRSMAHRAPA-VWDRTDAASGMLYIRASSPNEPEPSYVWTTFST-RSSPVTR 302 +S + R +W++ H +P + + +Y R+SS + Y + + R+ + Sbjct: 539 MSVLNRLLWKN-EHGSPKDIRSAASTPANPVYNRSSS--QTSKGYNSSDYDLLRTHSLDE 595 Query: 301 TSKTPFSMT*KLSPSSPV*LRTRRARISFRTSHPEPAAFVRVKRTEQQYAPD-RLSETGS 125 +P S++ S SSP T + S T+ P+ F+R+ E R S+T S Sbjct: 596 NVNSPTSISSPSSKSSPFTKTTSKDTKSATTTDERPSDFIRLNSEESVGTSSLRTSQTTS 655 Query: 124 LCV 116 V Sbjct: 656 TIV 658 >SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.8 bits (54), Expect = 3.5 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 66 PKRMILTKEPLPCSPSRTHREPVSLRRSG 152 P R +++PLP SPSRT + + SG Sbjct: 78 PVRSPSSRKPLPASPSRTRDHSLRVPVSG 106 >SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1079 Score = 24.6 bits (51), Expect = 8.2 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = -2 Query: 335 HLFDAVVSGDQDVENAVLDDVEVVAVIPCLI 243 HLFDA + + + ++ VAV PC++ Sbjct: 917 HLFDAFTAHQKKILEQKREESSDVAVFPCVL 947 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,988,129 Number of Sequences: 5004 Number of extensions: 40905 Number of successful extensions: 169 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 152 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 166 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 192109570 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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