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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0400X.Seq
         (492 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subun...    59   3e-10
SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy...    30   0.16 
SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S...    28   0.66 
SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr...    26   2.7  
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S...    26   3.5  
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo...    25   8.2  

>SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subunit
           Cgs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 412

 Score = 59.3 bits (137), Expect = 3e-10
 Identities = 38/91 (41%), Positives = 57/91 (62%), Gaps = 11/91 (12%)
 Frame = +3

Query: 252 GDDGDNFYVIENGVFDVLVTGDDRV--EKVVHTYEGS-------GS-FGELALMYNMPLA 401
           G  GD FY++E G FDV    +  +  E+V+ +  G+       G  FGELALMYN P A
Sbjct: 175 GAVGDYFYIVEQGEFDVYKRPELNITPEEVLSSGYGNYITTISPGEYFGELALMYNAPRA 234

Query: 402 ASVRSQTA-GALWAMDRHTFRRILLKSAFKK 491
           ASV S+T    ++A+DR +FRRI+ ++A+++
Sbjct: 235 ASVVSKTPNNVIYALDRTSFRRIVFENAYRQ 265



 Score = 45.6 bits (103), Expect = 4e-06
 Identities = 28/73 (38%), Positives = 42/73 (57%)
 Frame = +3

Query: 252 GDDGDNFYVIENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMYNMPLAASVRSQTAGA 431
           GD G+ FY+IE+G  +V+  G    + VV T      FGELAL++     A+V+++T   
Sbjct: 309 GDIGNQFYLIEDGEAEVVKNG----KGVVVTLTKGDYFGELALIHETVRNATVQAKTRLK 364

Query: 432 LWAMDRHTFRRIL 470
           L   D+ TF R+L
Sbjct: 365 LATFDKPTFNRLL 377



 Score = 25.0 bits (52), Expect = 6.2
 Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
 Frame = +2

Query: 32  RRKSVFAETYDPEEDDSDEGAPAVFPKSDAQRA-RLAEAVRGILLFRSLD 178
           RR+SV  E+ +P     +      FP  D +   RL  +V G  LF++LD
Sbjct: 101 RRQSVSTESMNPSAFALE--TKRTFPPKDPEDLKRLKRSVAGNFLFKNLD 148


>SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 348

 Score = 30.3 bits (65), Expect = 0.16
 Identities = 13/40 (32%), Positives = 24/40 (60%)
 Frame = +3

Query: 324 VEKVVHTYEGSGSFGELALMYNMPLAASVRSQTAGALWAM 443
           ++  +HTY+G  S  +  L+ ++PL++   S TA  L +M
Sbjct: 267 IDSFIHTYDGFSSMTQCLLIISLPLSSLWASSTALKLQSM 306


>SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1206

 Score = 28.3 bits (60), Expect = 0.66
 Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
 Frame = +3

Query: 279 IENGVFDVLVTGDDRVEKVVHTYEGSGSFGELALMY--NMPLAASVRSQTAGALW 437
           ++   F +L TGD  + K+   ++G G+  EL L Y   +PLA  +     G L+
Sbjct: 313 MKGSFFYLLQTGDGDLLKLTIEHDGQGNVVELRLKYFDTVPLAVQLNILKTGFLF 367


>SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 872

 Score = 26.2 bits (55), Expect = 2.7
 Identities = 29/123 (23%), Positives = 53/123 (43%), Gaps = 3/123 (2%)
 Frame = -3

Query: 475 LSRMRRNVWRSMAHRAPA-VWDRTDAASGMLYIRASSPNEPEPSYVWTTFST-RSSPVTR 302
           +S + R +W++  H +P  +       +  +Y R+SS  +    Y  + +   R+  +  
Sbjct: 539 MSVLNRLLWKN-EHGSPKDIRSAASTPANPVYNRSSS--QTSKGYNSSDYDLLRTHSLDE 595

Query: 301 TSKTPFSMT*KLSPSSPV*LRTRRARISFRTSHPEPAAFVRVKRTEQQYAPD-RLSETGS 125
              +P S++   S SSP    T +   S  T+   P+ F+R+   E       R S+T S
Sbjct: 596 NVNSPTSISSPSSKSSPFTKTTSKDTKSATTTDERPSDFIRLNSEESVGTSSLRTSQTTS 655

Query: 124 LCV 116
             V
Sbjct: 656 TIV 658


>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 25.8 bits (54), Expect = 3.5
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +3

Query: 66  PKRMILTKEPLPCSPSRTHREPVSLRRSG 152
           P R   +++PLP SPSRT    + +  SG
Sbjct: 78  PVRSPSSRKPLPASPSRTRDHSLRVPVSG 106


>SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1079

 Score = 24.6 bits (51), Expect = 8.2
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = -2

Query: 335  HLFDAVVSGDQDVENAVLDDVEVVAVIPCLI 243
            HLFDA  +  + +     ++   VAV PC++
Sbjct: 917  HLFDAFTAHQKKILEQKREESSDVAVFPCVL 947


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,988,129
Number of Sequences: 5004
Number of extensions: 40905
Number of successful extensions: 169
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 192109570
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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