Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0368.Seq
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 141 7e-35
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 91 2e-19
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 89 7e-19
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 67 2e-12
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 27 1.9
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 27 3.3
SPBC365.12c |ish1||LEA domain protein|Schizosaccharomyces pombe|... 27 3.3
SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 26 4.3
SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 26 4.3
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 26 5.7
SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyce... 26 5.7
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 26 5.7
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 25 7.6
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 7.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 141 bits (342), Expect = 7e-35
Identities = 62/82 (75%), Positives = 73/82 (89%)
Frame = +1
Query: 256 ELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 435
ELTQQMFDA NMM A DPRHGRYLTVAA+FRG++SMKEVDEQ+ ++Q KNS+YFVEWIP+
Sbjct: 288 ELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPD 347
Query: 436 NVKTAVCDIPPRGLKMAATFIG 501
NV AVC +PP+ LKM+ATFIG
Sbjct: 348 NVLKAVCSVPPKDLKMSATFIG 369
Score = 126 bits (305), Expect = 2e-30
Identities = 58/85 (68%), Positives = 69/85 (81%)
Frame = +2
Query: 2 DNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPR 181
DNEAL I TLK+ +P+Y DLNHLVS M+GVTT RFPG+LN+DLRKLAVNMVPFPR
Sbjct: 203 DNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPR 262
Query: 182 LHFFMPGFAPLTSRGSRQYRALTVP 256
LHFFM GFAPL + GS ++A++VP
Sbjct: 263 LHFFMVGFAPLAAIGSSSFQAVSVP 287
Score = 111 bits (267), Expect = 9e-26
Identities = 48/55 (87%), Positives = 54/55 (98%)
Frame = +3
Query: 510 AIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 674
+IQE+F+R+ +QF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE
Sbjct: 373 SIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 90.6 bits (215), Expect = 2e-19
Identities = 39/94 (41%), Positives = 57/94 (60%)
Frame = +2
Query: 2 DNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPR 181
DNEA YDIC R L + PTY +LN L++ +S +T LRF G LN DL + N+VP+PR
Sbjct: 209 DNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPR 268
Query: 182 LHFFMPGFAPLTSRGSRQYRALTVPSSRNRCSTP 283
+HF + ++P+ S + + +V N+C P
Sbjct: 269 IHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEP 302
Score = 65.3 bits (152), Expect = 8e-12
Identities = 25/72 (34%), Positives = 42/72 (58%)
Frame = +1
Query: 256 ELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 435
E+T Q F+ N M CDPR GRY+ ++RG + ++V + +I+++ + FV+W P
Sbjct: 294 EITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPT 353
Query: 436 NVKTAVCDIPPR 471
K +C PP+
Sbjct: 354 GFKIGICYEPPQ 365
Score = 56.0 bits (129), Expect = 5e-09
Identities = 21/52 (40%), Positives = 36/52 (69%)
Frame = +3
Query: 510 AIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 665
+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 387 SIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 438
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 88.6 bits (210), Expect = 7e-19
Identities = 38/94 (40%), Positives = 57/94 (60%)
Frame = +2
Query: 2 DNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPR 181
DNE+ YDIC R L + P+Y +LN L++ +S +T LRF G LN DL + N+VP+PR
Sbjct: 205 DNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPR 264
Query: 182 LHFFMPGFAPLTSRGSRQYRALTVPSSRNRCSTP 283
+HF + +AP+ S + + +V N+C P
Sbjct: 265 IHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEP 298
Score = 68.9 bits (161), Expect = 6e-13
Identities = 27/75 (36%), Positives = 43/75 (57%)
Frame = +1
Query: 256 ELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 435
E+T Q F+ N M CDPR GRY+ ++RG + ++V + I+ K + FV+W P
Sbjct: 290 EITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPT 349
Query: 436 NVKTAVCDIPPRGLK 480
K +CD PP+ ++
Sbjct: 350 GFKIGICDRPPQHIE 364
Score = 56.0 bits (129), Expect = 5e-09
Identities = 21/52 (40%), Positives = 36/52 (69%)
Frame = +3
Query: 510 AIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 665
+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 383 SIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 434
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 67.3 bits (157), Expect = 2e-12
Identities = 31/82 (37%), Positives = 45/82 (54%)
Frame = +2
Query: 2 DNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPR 181
DN AL I L PT+ N LVS MS TT LR+PG +N DL + +++P PR
Sbjct: 206 DNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPR 265
Query: 182 LHFFMPGFAPLTSRGSRQYRAL 247
HF + + P T++ + +A+
Sbjct: 266 CHFLLTSYTPFTNQQVEEAKAI 287
Score = 37.1 bits (82), Expect = 0.002
Identities = 19/58 (32%), Positives = 34/58 (58%), Gaps = 3/58 (5%)
Frame = +3
Query: 510 AIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMN---DLVSEYQQYQE 674
+I LFKR +Q+ + +R AFL Y E + E + E +S+ + DL++EY+ ++
Sbjct: 383 SIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYEACED 440
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 27.5 bits (58), Expect = 1.9
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = +3
Query: 507 HAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 671
H + L+K + E+F+ +F RK L WY G+ E + N+N SE + Q
Sbjct: 1707 HLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
orphan|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1563
Score = 26.6 bits (56), Expect = 3.3
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +3
Query: 3 TTRLYTTSASALSNCPHPPTVTLTT 77
T + TTS S ++CP P T+ + T
Sbjct: 174 TVLIVTTSGSTSTSCPPPTTILIVT 198
>SPBC365.12c |ish1||LEA domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 684
Score = 26.6 bits (56), Expect = 3.3
Identities = 20/74 (27%), Positives = 30/74 (40%)
Frame = +3
Query: 432 QQREDRRVRHSPSWSQDGRHLHRKLHAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEM 611
Q+ RR R W+ G + H I +QF A RR+ +L + +
Sbjct: 287 QEISKRRGRFGNWWANSGLKAYFDAHGIPAYQPSPIDQFYAHLRRQYYLKTNGYQALKSK 346
Query: 612 EFTEAESNMNDLVS 653
+ EA SN+ D S
Sbjct: 347 AYEEA-SNVADSAS 359
>SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC
subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 962
Score = 26.2 bits (55), Expect = 4.3
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = -3
Query: 425 HSTKYEEFLFWMLSICSSTSFMDMRPRKMAATV 327
+ST +E+ + W++++ SST MRP + ATV
Sbjct: 199 NSTVFEDIMSWVVAMSSST----MRPIRHTATV 227
>SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex
subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1522
Score = 26.2 bits (55), Expect = 4.3
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +2
Query: 392 SKTRTPHTSWNGSPTT*RPPCATFPLVVSRWPPPSSETPRHPGAVQ 529
S T TP S + +PT+ PP F + P+ E R AVQ
Sbjct: 434 SATATPKRSPSATPTSALPPIGKFAPPTTAKAQPAPEKRRGEPAVQ 479
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 25.8 bits (54), Expect = 5.7
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +3
Query: 462 SPSWSQDGRHLHRKLHAIQELF 527
S WS D +LH HA QE+F
Sbjct: 342 SLGWSVDDSNLHEVYHANQEVF 363
>SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 399
Score = 25.8 bits (54), Expect = 5.7
Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +1
Query: 319 RYLTVAAIFRGRMSMKEVDEQMLNIQNKN--SSYFVEWIPNNVK 444
RYLT + + +++K V + +LN N++ + F+ W P K
Sbjct: 297 RYLTGKVVEQEYLTVKLVSKTLLNFSNQSLCKAVFIVWDPPGSK 340
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 25.8 bits (54), Expect = 5.7
Identities = 11/53 (20%), Positives = 26/53 (49%)
Frame = +1
Query: 340 IFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFI 498
++ + ++ + + ++NI+ N S W+PN + +P G K++ I
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLI 395
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.4 bits (53), Expect = 7.6
Identities = 11/39 (28%), Positives = 17/39 (43%)
Frame = +1
Query: 319 RYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 435
+Y+ ++F G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.4 bits (53), Expect = 7.6
Identities = 20/77 (25%), Positives = 37/77 (48%), Gaps = 5/77 (6%)
Frame = +3
Query: 24 SASALSNCPHPPTVTLTT*FPSQCLVSPLALGSPVS*TLISENW--P*TWFPS---RVST 188
+A +LS CP PT L++ + +V + G + S NW +W PS RV+
Sbjct: 262 AALSLSWCPEDPTFLLSSGKDGRAMVWNVETGESLGSFPRSGNWYTKSSWCPSNSNRVAV 321
Query: 189 SSCQVSLPSHPVEADST 239
+S + + +++ +T
Sbjct: 322 ASLEGKVSIFSIQSTNT 338
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,851,456
Number of Sequences: 5004
Number of extensions: 58174
Number of successful extensions: 204
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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