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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0359.Seq
         (628 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    27   2.2  
SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz...    26   3.9  
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom...    26   3.9  
SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact...    26   5.1  
SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces...    26   5.1  
SPBC11G11.04 |trs20||TRAPP complex subunit Trs20 |Schizosaccharo...    26   5.1  
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc...    25   6.8  
SPAC637.10c |rpn10|pus1|19S proteasome regulatory subunit Rpn10|...    25   6.8  
SPAC31A2.12 |||arrestin/PY protein 1|Schizosaccharomyces pombe|c...    25   6.8  
SPBP8B7.17c |||phosphomethylpyrimidine kinase|Schizosaccharomyce...    25   9.0  
SPAC24B11.06c |sty1|spc1, phh1|MAP kinase Sty1|Schizosaccharomyc...    25   9.0  

>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1610

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
 Frame = +2

Query: 296 KNTNFFISTLVTYVLTYHLGWVST-VSPYDTEVHQTAATKSNDSKRPYNVLWAQL 457
           K  +  IS L +      L  +S  +S +  EVHQ A    NDSKRP + L++ L
Sbjct: 299 KRVSRMISRLTSLTCVPMLRLLSNMISNFPNEVHQFA----NDSKRPLSKLFSNL 349


>SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 900

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 16/43 (37%), Positives = 24/43 (55%)
 Frame = -1

Query: 559 VKITSSLLTNWVLVAQL**CERARWKTNISAEIIELCPQNVVR 431
           +KI  SLL NW+L   L     A+  +    E +EL P+NV++
Sbjct: 464 MKIQKSLLVNWLLELML-----AKLNSLDEKERLELFPENVMQ 501


>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 166

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 9/33 (27%), Positives = 17/33 (51%)
 Frame = +3

Query: 228 GASNKMAESCCPISVPYWPLETRRIQTFSSVHL 326
           G   +M  +   + +P+W  E   I +F S+H+
Sbjct: 28  GHEGRMVPTGSKVELPFWLAEVLAINSFVSIHM 60


>SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor
           complex associated protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 710

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 12/48 (25%), Positives = 25/48 (52%)
 Frame = -1

Query: 202 NHIGCKRGPYIRSVSHLAASSAARCKVDTKAPPVPAASIASLNLHTMA 59
           NH      PY+ + SH + +  +   ++T +  VP +S + +N+  +A
Sbjct: 129 NHTSISANPYVNNPSHTSRNPDSGSSLNTASHEVP-SSKSDVNVQMLA 175


>SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 618

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 13/31 (41%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
 Frame = +3

Query: 108 GAFVSTLHRA-AEDAAKWLTDLMYGPRLQPM 197
           G F+S L     +DAA W   L+Y  RL P+
Sbjct: 491 GQFLSCLPAPKVDDAANWNAGLLYSKRLGPV 521


>SPBC11G11.04 |trs20||TRAPP complex subunit Trs20
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 136

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 19/64 (29%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
 Frame = -2

Query: 465 RSLSCAHRTLYGRLESFDFVAAVWWTSVS*GL-TVDTHPRWYVSTYVTSVLMKKFVFFVS 289
           RSL+ +H   +    S D V  + WTS +  + T+D     Y+S YVT   M+  +   +
Sbjct: 29  RSLN-SHLNQFIVHSSLDIVDQLQWTSNAFYMKTIDQFHEMYISAYVTPSNMRFMLLHQN 87

Query: 288 PTAN 277
            +A+
Sbjct: 88  QSAD 91


>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1679

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 9/37 (24%), Positives = 21/37 (56%)
 Frame = +2

Query: 254 VLSDLCSVLAVGDTKNTNFFISTLVTYVLTYHLGWVS 364
           VLS   ++++  + KN NFF +     ++ Y + +++
Sbjct: 645 VLSSCFTIISTSNNKNDNFFKAETALLIIGYTISYLA 681


>SPAC637.10c |rpn10|pus1|19S proteasome regulatory subunit
           Rpn10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 243

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 13/41 (31%), Positives = 20/41 (48%)
 Frame = +2

Query: 416 NDSKRPYNVLWAQLNDLCGNIGFPPRALALSQLRDQHPVRQ 538
           N+S   + +  A  +D C  +  PP    LS L +Q P+ Q
Sbjct: 149 NESALQHFIDAANSSDSCHLVSIPPSPQLLSDLVNQSPIGQ 189


>SPAC31A2.12 |||arrestin/PY protein 1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 596

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 17/44 (38%), Positives = 24/44 (54%)
 Frame = -2

Query: 300 FFVSPTANTEQRSDSTTRPFYSKLRASHS*LDSTTSAANGARTS 169
           FFVSPT +T Q  DS  R  +    +S S +  + S+A+ A  S
Sbjct: 511 FFVSPTESTRQSLDS--RRSFEHSTSSSSGISPSHSSASLAHLS 552


>SPBP8B7.17c |||phosphomethylpyrimidine kinase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 506

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 9/33 (27%), Positives = 18/33 (54%)
 Frame = +3

Query: 114 FVSTLHRAAEDAAKWLTDLMYGPRLQPMWLNLV 212
           + S L R   +  +++  + Y P + P WL++V
Sbjct: 277 YASALTRLPYEKGEFINFVRYHPSITPKWLSIV 309


>SPAC24B11.06c |sty1|spc1, phh1|MAP kinase Sty1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 349

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 10/20 (50%), Positives = 11/20 (55%)
 Frame = -2

Query: 522 WSRNCDSASARGGKPIFPQR 463
           WS  C  A    GKP+FP R
Sbjct: 198 WSAGCIFAEMIEGKPLFPGR 217


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,839,597
Number of Sequences: 5004
Number of extensions: 59281
Number of successful extensions: 191
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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