BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0359.Seq
(628 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 27 2.2
SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz... 26 3.9
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 26 3.9
SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact... 26 5.1
SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces... 26 5.1
SPBC11G11.04 |trs20||TRAPP complex subunit Trs20 |Schizosaccharo... 26 5.1
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 25 6.8
SPAC637.10c |rpn10|pus1|19S proteasome regulatory subunit Rpn10|... 25 6.8
SPAC31A2.12 |||arrestin/PY protein 1|Schizosaccharomyces pombe|c... 25 6.8
SPBP8B7.17c |||phosphomethylpyrimidine kinase|Schizosaccharomyce... 25 9.0
SPAC24B11.06c |sty1|spc1, phh1|MAP kinase Sty1|Schizosaccharomyc... 25 9.0
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 27.1 bits (57), Expect = 2.2
Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Frame = +2
Query: 296 KNTNFFISTLVTYVLTYHLGWVST-VSPYDTEVHQTAATKSNDSKRPYNVLWAQL 457
K + IS L + L +S +S + EVHQ A NDSKRP + L++ L
Sbjct: 299 KRVSRMISRLTSLTCVPMLRLLSNMISNFPNEVHQFA----NDSKRPLSKLFSNL 349
>SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 900
Score = 26.2 bits (55), Expect = 3.9
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = -1
Query: 559 VKITSSLLTNWVLVAQL**CERARWKTNISAEIIELCPQNVVR 431
+KI SLL NW+L L A+ + E +EL P+NV++
Sbjct: 464 MKIQKSLLVNWLLELML-----AKLNSLDEKERLELFPENVMQ 501
>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 166
Score = 26.2 bits (55), Expect = 3.9
Identities = 9/33 (27%), Positives = 17/33 (51%)
Frame = +3
Query: 228 GASNKMAESCCPISVPYWPLETRRIQTFSSVHL 326
G +M + + +P+W E I +F S+H+
Sbjct: 28 GHEGRMVPTGSKVELPFWLAEVLAINSFVSIHM 60
>SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor
complex associated protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 710
Score = 25.8 bits (54), Expect = 5.1
Identities = 12/48 (25%), Positives = 25/48 (52%)
Frame = -1
Query: 202 NHIGCKRGPYIRSVSHLAASSAARCKVDTKAPPVPAASIASLNLHTMA 59
NH PY+ + SH + + + ++T + VP +S + +N+ +A
Sbjct: 129 NHTSISANPYVNNPSHTSRNPDSGSSLNTASHEVP-SSKSDVNVQMLA 175
>SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 618
Score = 25.8 bits (54), Expect = 5.1
Identities = 13/31 (41%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +3
Query: 108 GAFVSTLHRA-AEDAAKWLTDLMYGPRLQPM 197
G F+S L +DAA W L+Y RL P+
Sbjct: 491 GQFLSCLPAPKVDDAANWNAGLLYSKRLGPV 521
>SPBC11G11.04 |trs20||TRAPP complex subunit Trs20
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 136
Score = 25.8 bits (54), Expect = 5.1
Identities = 19/64 (29%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
Frame = -2
Query: 465 RSLSCAHRTLYGRLESFDFVAAVWWTSVS*GL-TVDTHPRWYVSTYVTSVLMKKFVFFVS 289
RSL+ +H + S D V + WTS + + T+D Y+S YVT M+ + +
Sbjct: 29 RSLN-SHLNQFIVHSSLDIVDQLQWTSNAFYMKTIDQFHEMYISAYVTPSNMRFMLLHQN 87
Query: 288 PTAN 277
+A+
Sbjct: 88 QSAD 91
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 25.4 bits (53), Expect = 6.8
Identities = 9/37 (24%), Positives = 21/37 (56%)
Frame = +2
Query: 254 VLSDLCSVLAVGDTKNTNFFISTLVTYVLTYHLGWVS 364
VLS ++++ + KN NFF + ++ Y + +++
Sbjct: 645 VLSSCFTIISTSNNKNDNFFKAETALLIIGYTISYLA 681
>SPAC637.10c |rpn10|pus1|19S proteasome regulatory subunit
Rpn10|Schizosaccharomyces pombe|chr 1|||Manual
Length = 243
Score = 25.4 bits (53), Expect = 6.8
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +2
Query: 416 NDSKRPYNVLWAQLNDLCGNIGFPPRALALSQLRDQHPVRQ 538
N+S + + A +D C + PP LS L +Q P+ Q
Sbjct: 149 NESALQHFIDAANSSDSCHLVSIPPSPQLLSDLVNQSPIGQ 189
>SPAC31A2.12 |||arrestin/PY protein 1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 596
Score = 25.4 bits (53), Expect = 6.8
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = -2
Query: 300 FFVSPTANTEQRSDSTTRPFYSKLRASHS*LDSTTSAANGARTS 169
FFVSPT +T Q DS R + +S S + + S+A+ A S
Sbjct: 511 FFVSPTESTRQSLDS--RRSFEHSTSSSSGISPSHSSASLAHLS 552
>SPBP8B7.17c |||phosphomethylpyrimidine kinase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 506
Score = 25.0 bits (52), Expect = 9.0
Identities = 9/33 (27%), Positives = 18/33 (54%)
Frame = +3
Query: 114 FVSTLHRAAEDAAKWLTDLMYGPRLQPMWLNLV 212
+ S L R + +++ + Y P + P WL++V
Sbjct: 277 YASALTRLPYEKGEFINFVRYHPSITPKWLSIV 309
>SPAC24B11.06c |sty1|spc1, phh1|MAP kinase Sty1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 349
Score = 25.0 bits (52), Expect = 9.0
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = -2
Query: 522 WSRNCDSASARGGKPIFPQR 463
WS C A GKP+FP R
Sbjct: 198 WSAGCIFAEMIEGKPLFPGR 217
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,839,597
Number of Sequences: 5004
Number of extensions: 59281
Number of successful extensions: 191
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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