Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0347X.Seq
(434 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1223.08c |dfr1||dihydrofolate reductase Dfr1|Schizosaccharom... 31 0.077
SPAC1F3.07c |rsc58||RSC complex subunit Rsc58|Schizosaccharomyce... 29 0.31
SPCC63.08c |ppk36|atg1|serine/threonine protein kinase Ppk36|Sch... 27 1.6
SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ... 26 2.2
SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo... 26 2.9
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 2.9
SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 26 2.9
SPAC23C11.05 |||inorganic pyrophosphatase |Schizosaccharomyces p... 25 5.0
SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyc... 25 5.0
SPBC660.05 |||conserved fungal protein|Schizosaccharomyces pombe... 25 6.7
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 25 6.7
SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz... 25 6.7
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.7
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 25 6.7
SPCC1672.08c |tfa2||transcription factor TFIIE beta subunit Tfa2... 24 8.8
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 24 8.8
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 24 8.8
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 24 8.8
>SPCC1223.08c |dfr1||dihydrofolate reductase
Dfr1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 461
Score = 31.1 bits (67), Expect = 0.077
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 74 PKVTSPVLVIHGTEDEVIDFSHGLAIYERCPRA 172
PK+T+P L I GT D ++ + + ERC A
Sbjct: 162 PKLTTPSLHIAGTSDTLVPLARSKQLVERCENA 194
>SPAC1F3.07c |rsc58||RSC complex subunit Rsc58|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 403
Score = 29.1 bits (62), Expect = 0.31
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = -3
Query: 222 NSSTSLCPAPSTHSGSTARGHRSYMASPWEKSI 124
NSS+S+ P S HS + R Y +P EKSI
Sbjct: 218 NSSSSVIPDSSFHSVACYRASSHYKEAPVEKSI 250
>SPCC63.08c |ppk36|atg1|serine/threonine protein kinase
Ppk36|Schizosaccharomyces pombe|chr 3|||Manual
Length = 830
Score = 26.6 bits (56), Expect = 1.6
Identities = 23/73 (31%), Positives = 30/73 (41%), Gaps = 5/73 (6%)
Frame = -1
Query: 317 SSPCVVWPVACSLCQLSNSTDQNVSVSRGTD*TVPHRYAQHP-----LPIVAPLHADTAH 153
S+P P L Q + ++ S T VPHRYAQ P+ P A A
Sbjct: 346 SNPGAPAPPGGLLRQAFQAQGSSIQPSEITGRRVPHRYAQDGNTLPYTPVFPPESAPAAS 405
Query: 152 IWRVRGRSQLPHP 114
I+ R S+ P P
Sbjct: 406 IFPPRLTSKQPIP 418
>SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 2.2
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +2
Query: 152 YERCPRAVEPLWVEGAGHNDVELFNQYLERLKR 250
Y+ ++++ L + ++VE FN+YL RLK+
Sbjct: 624 YDLALQSLQSLRLHSILEDEVERFNEYLTRLKK 656
>SPBC16D10.07c |sir2||Sir2 family histone deacetylase
Sir2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 25.8 bits (54), Expect = 2.9
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = -3
Query: 225 LNSSTSLCPAPSTHSGSTARGHRSYMASPWEKSIT 121
LN +LC P HSGS R + + +E+ T
Sbjct: 430 LNELQALCDLPECHSGSKTRAFETDLDIKFEEPST 464
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.8 bits (54), Expect = 2.9
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -1
Query: 347 PELNVDAGFCSSPCVVWPVACSLCQL 270
PE+ + G+ S PC VW + C L +L
Sbjct: 548 PEIILGLGW-SYPCDVWSIGCILVEL 572
>SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 378
Score = 25.8 bits (54), Expect = 2.9
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +2
Query: 59 SIDKIPKVTSPVLVIHGTEDEVIDFSHGLAIYE 157
+I+ P+ P+L+ HGT+D V YE
Sbjct: 200 TIELAPQFFLPLLITHGTDDNVTCSDSSKEFYE 232
>SPAC23C11.05 |||inorganic pyrophosphatase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 289
Score = 25.0 bits (52), Expect = 5.0
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = +2
Query: 5 MRVAFPNTKRTWFFDAFPSIDKIPKVTSPVLVIHGTED 118
+R FP+ W + AFP + P V P G D
Sbjct: 82 VRNCFPHHGYIWNYGAFPQTYEDPNVVHPETKAKGDSD 119
>SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 313
Score = 25.0 bits (52), Expect = 5.0
Identities = 14/53 (26%), Positives = 23/53 (43%)
Frame = -1
Query: 206 YAQHPLPIVAPLHADTAHIWRVRGRSQLPHPRFHESQELEK*LWVFCQYWEMH 48
Y QH + + + HI + R HP FHE+ + + + C Y + H
Sbjct: 244 YVQHFKCNIGTIRHNYPHINQWLKRLYWKHPAFHETTDFKH---IKCHYTQSH 293
>SPBC660.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 143
Score = 24.6 bits (51), Expect = 6.7
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -3
Query: 183 SGSTARGHRSYMASP 139
+GS RGHR+Y A P
Sbjct: 86 AGSVLRGHRAYYAPP 100
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 24.6 bits (51), Expect = 6.7
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3
Query: 339 QCGCRILFITLCCLASGLQSLSVEQL 262
QCG L TLC L +G + L+++ L
Sbjct: 706 QCGTDALRFTLCSLTTGGRDLNLDIL 731
>SPBC354.05c |sre2||membrane-tethered transcription factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 793
Score = 24.6 bits (51), Expect = 6.7
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = -3
Query: 240 LSRY*LNSS--TSLCPAPSTHSGSTARGHRSYMASPWEKSI 124
L+R ++SS T L P S H + A+ H +S W+ SI
Sbjct: 106 LARNSIDSSINTELGPLESNHWFTQAQDHSRQYSSSWDPSI 146
>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 24.6 bits (51), Expect = 6.7
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -3
Query: 219 SSTSLCPAPSTHSGSTARGHRSYMASPWEKSITSS 115
SSTS A + S +T+ S ++P SITSS
Sbjct: 140 SSTSSSTATPSSSSTTSSSSSSSSSTPISSSITSS 174
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 24.6 bits (51), Expect = 6.7
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -2
Query: 109 SMNHKNWRSNFGYFVNTGKCIEKPSSLGVWKCYSH 5
S+N SNF Y T K +E +++GV + +SH
Sbjct: 269 SVNESRIVSNFLYIPLTDKEMEAINNIGVVRRFSH 303
>SPCC1672.08c |tfa2||transcription factor TFIIE beta subunit
Tfa2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 285
Score = 24.2 bits (50), Expect = 8.8
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +2
Query: 125 IDFSHGLAIYERCPRAVEPLWVEGAGHN 208
+D + L Y P +V+P V+ AGHN
Sbjct: 227 LDLASELGKYGLKPTSVDPSTVKRAGHN 254
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 24.2 bits (50), Expect = 8.8
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = -2
Query: 151 YGESVGEVNYLILGSMNHKNWRSNFGYFVNTGKCI 47
YG S+ N L++ W + FG F N +CI
Sbjct: 722 YGYSLSSPNALVV-------WEAQFGDFANNAQCI 749
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 24.2 bits (50), Expect = 8.8
Identities = 17/72 (23%), Positives = 34/72 (47%)
Frame = -3
Query: 219 SSTSLCPAPSTHSGSTARGHRSYMASPWEKSITSSSVP*ITRTGEVTLGILSILGNASKN 40
SST L + + S S++ S ++SP ++SSS + + + + I +I S +
Sbjct: 508 SSTLLSGSSAIPSTSSSTPSSSIISSPMTSVLSSSSSIPTSSSSDFSSSITTISSGISSS 567
Query: 39 QVLLVFGNATRI 4
+ F + + I
Sbjct: 568 SIPSTFSSVSSI 579
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 24.2 bits (50), Expect = 8.8
Identities = 22/82 (26%), Positives = 36/82 (43%), Gaps = 4/82 (4%)
Frame = +2
Query: 2 GMRVAFPNTKRTWFFDAFPSIDKIPKVT----SPVLVIHGTEDEVIDFSHGLAIYERCPR 169
G +AFP + R++ D D+IP T + +I G+ D ++ H L I +
Sbjct: 311 GSILAFPRSCRSFALDGSFHSDEIPNFTMSGAAYARIIIGSHDYMVQ-QHLLEIEQLQQI 369
Query: 170 AVEPLWVEGAGHNDVELFNQYL 235
A E + + G+ E Q L
Sbjct: 370 AAEDISLYGSADTYYERITQIL 391
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,949,289
Number of Sequences: 5004
Number of extensions: 41264
Number of successful extensions: 122
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 156095170
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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