Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0335.Seq
         (768 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosacch...   109   4e-25
SPCC306.05c |ins1||INSIG domain protein|Schizosaccharomyces pomb...    29   0.97 
SPAC110.02 |pds5||cohesin-associated protein Pds5|Schizosaccharo...    28   1.7  
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce...    27   2.2  
SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|c...    26   6.8  
SPCC70.06 |||nuclear export factor|Schizosaccharomyces pombe|chr...    25   9.0  
SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch...    25   9.0  

>SPBC14F5.05c |sam1||S-adenosylmethionine synthetase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 382

 Score =  109 bits (262), Expect = 4e-25
 Identities = 51/78 (65%), Positives = 57/78 (73%)
 Frame = +3

Query: 255 PAQYLDERTVIHINPCGLFIIGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVD 434
           PA  LDE+TV HI P G F++GGPQ DAGLTGRKIIVDTY             KD++KVD
Sbjct: 219 PAHLLDEKTVYHIQPSGRFVVGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYSKVD 278

Query: 435 RSAAYAARWVAKSLVKAG 488
           RSAAYAARW+AKSLV AG
Sbjct: 279 RSAAYAARWIAKSLVAAG 296



 Score = 99.5 bits (237), Expect = 5e-22
 Identities = 46/89 (51%), Positives = 63/89 (70%)
 Frame = +1

Query: 4   ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSL 183
           E +PLT++LAHKLN  ++  RR+G   W RPD+KTQVT EY    GA +P+RV T+VVS 
Sbjct: 135 EKLPLTILLAHKLNAAMSVARRDGSLPWLRPDTKTQVTIEYEEENGAVIPRRVDTIVVSA 194

Query: 184 QHSEKITLETLRDEIREKVIKEVILHNIL 270
           QH++ I+ E LR EI EK+IK  +  ++L
Sbjct: 195 QHADSISTEDLRSEILEKIIKPTVPAHLL 223



 Score = 97.5 bits (232), Expect = 2e-21
 Identities = 45/77 (58%), Positives = 55/77 (71%)
 Frame = +2

Query: 509 QVAYAISVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRAPIYQRTST 688
           Q++YAI VAEPLSI V  YGTS KT  +L+ I++KNFDLRPG +VK L L+ P Y  T++
Sbjct: 304 QLSYAIGVAEPLSIFVNTYGTSSKTSAELVEIIRKNFDLRPGVLVKSLKLQTPFYLSTAS 363

Query: 689 YGHFGRAGFPWEIPKPL 739
           YGHF     PWE PK L
Sbjct: 364 YGHFTDQSKPWEQPKEL 380


>SPCC306.05c |ins1||INSIG domain protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 281

 Score = 28.7 bits (61), Expect = 0.97
 Identities = 15/56 (26%), Positives = 29/56 (51%)
 Frame = -2

Query: 365 HDDFASRQARV*LRSSNYKESTWINVNYSSLIKILCRITSLMTFSLISSRKVSRVI 198
           ++D+ASRQ    L+    K  T+I+  YSSLI   C++  +     + +    +++
Sbjct: 62  YEDYASRQIDDDLKKQRKKGITFID--YSSLITFFCKLCVIFGLGFVFTYLAEQIV 115


>SPAC110.02 |pds5||cohesin-associated protein
           Pds5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1205

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
 Frame = -1

Query: 168 SMNSLWDCCTTSKYIFASNLCF*IWSCPPK--FSISPELCNFL 46
           S +SL D  TT ++IF+ NL   +W   P    +I P+  N L
Sbjct: 244 SRDSLSDSMTTPEFIFSHNLVLQLWKYAPTTLLNIIPQFENEL 286


>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1210

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 23/90 (25%), Positives = 35/90 (38%), Gaps = 6/90 (6%)
 Frame = +2

Query: 380 VGGARRGRFLWQGLHQGRPLRSIRRPM------GRQVARQGGLVPVLHGQVAYAISVAEP 541
           +G +     + Q L  G P    + P        RQ   Q   V V+  QVA++I    P
Sbjct: 616 IGSSENAELIHQRLRNGLPKSIEKGPFIYNPFNRRQDQNQSKSVLVVDEQVAFSIYFRNP 675

Query: 542 LSITVFDYGTSHKTQQQLLSIVQKNFDLRP 631
           LS++V       +T+          F +RP
Sbjct: 676 LSVSVEVQDVHLETKGVSAKCSHSTFTMRP 705


>SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 6.8
 Identities = 12/39 (30%), Positives = 22/39 (56%)
 Frame = -2

Query: 341  ARV*LRSSNYKESTWINVNYSSLIKILCRITSLMTFSLI 225
            AR   +SS+Y++  W   N+S  I IL  + + + F ++
Sbjct: 984  ARFCTKSSHYRKKLWDFYNFSCTITILMLLLAPLDFPVL 1022


>SPCC70.06 |||nuclear export factor|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 458

 Score = 25.4 bits (53), Expect = 9.0
 Identities = 13/25 (52%), Positives = 16/25 (64%)
 Frame = -3

Query: 502 QHRHKPALTSDLATHRAAYAAERST 428
           +HR KP+L    +T RAA AA  ST
Sbjct: 12  KHRPKPSLDRSDSTKRAARAARFST 36


>SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 752

 Score = 25.4 bits (53), Expect = 9.0
 Identities = 14/42 (33%), Positives = 22/42 (52%)
 Frame = +1

Query: 583 SATTALHRTKEFRFTTGQNRQRVEPQSTNLSENKYLWTLWKG 708
           SA  A     +  F  G++   ++P S++L   KY+ TL KG
Sbjct: 255 SAIQAFESILQNTFFNGKSNPTIDPNSSSLLSEKYI-TLDKG 295


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,185,665
Number of Sequences: 5004
Number of extensions: 64146
Number of successful extensions: 178
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)