Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0335.Seq
(768 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 97 1e-20
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 97 2e-20
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 94 8e-20
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 94 8e-20
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 93 2e-19
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct... 28 6.0
At1g58230.1 68414.m06618 WD-40 repeat family protein / beige-rel... 28 6.0
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 97.1 bits (231), Expect = 1e-20
Identities = 49/97 (50%), Positives = 62/97 (63%)
Frame = +1
Query: 4 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSL 183
E MPLT VLA KL K+ E+R+NG W RPD KTQVT EY+ GA VP RVHTV++S
Sbjct: 136 ELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAMVPVRVHTVLIST 195
Query: 184 QHSEKITLETLRDEIREKVIKEVILHNILMREL*FTL 294
QH E +T + + +++E VIK VI L + F L
Sbjct: 196 QHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHL 232
Score = 95.9 bits (228), Expect = 3e-20
Identities = 44/78 (56%), Positives = 52/78 (66%)
Frame = +3
Query: 255 PAQYLDERTVIHINPCGLFIIGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVD 434
P +YLDE+T+ H+NP G F+IGGP DAGLTGRKII+DTY KD TKVD
Sbjct: 220 PEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVD 279
Query: 435 RSAAYAARWVAKSLVKAG 488
RS AY R AKS+V +G
Sbjct: 280 RSGAYIVRQAAKSIVASG 297
Score = 75.4 bits (177), Expect = 4e-14
Identities = 44/100 (44%), Positives = 60/100 (60%), Gaps = 8/100 (8%)
Frame = +2
Query: 464 RQVARQ---GGLVPVLHGQVAYAISVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPG 634
RQ A+ GL + QV+YAI V EPLS+ V YGT +++L IV+++FD RPG
Sbjct: 287 RQAAKSIVASGLARRVIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILEIVKESFDFRPG 346
Query: 635 KIVKELNLRA---PIYQRTSTYGHFGR--AGFPWEIPKPL 739
I L+L+ + +T+ YGHFGR A F WE+ KPL
Sbjct: 347 MISINLDLKRGGNGRFLKTAAYGHFGRDDADFTWEVVKPL 386
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 96.7 bits (230), Expect = 2e-20
Identities = 45/78 (57%), Positives = 52/78 (66%)
Frame = +3
Query: 255 PAQYLDERTVIHINPCGLFIIGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVD 434
PA+YLD+ T+ H+NP G F+IGGP DAGLTGRKII+DTY KD TKVD
Sbjct: 220 PAKYLDDNTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVD 279
Query: 435 RSAAYAARWVAKSLVKAG 488
RS AY R AKS+V AG
Sbjct: 280 RSGAYIVRQAAKSVVAAG 297
Score = 92.7 bits (220), Expect = 2e-19
Identities = 45/84 (53%), Positives = 57/84 (67%)
Frame = +1
Query: 4 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSL 183
E MPLT VLA KL K+ E+R+N W RPD KTQVT EY GGA +P RVHTV++S
Sbjct: 136 ELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAMIPIRVHTVLIST 195
Query: 184 QHSEKITLETLRDEIREKVIKEVI 255
QH E +T + + +++E VIK VI
Sbjct: 196 QHDETVTNDEIAADLKEHVIKPVI 219
Score = 67.3 bits (157), Expect = 1e-11
Identities = 35/82 (42%), Positives = 50/82 (60%), Gaps = 5/82 (6%)
Frame = +2
Query: 509 QVAYAISVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRAP---IYQR 679
QV+YAI V EPLS+ V Y T + +L ++++ FD RPG + L+L+ +Q+
Sbjct: 305 QVSYAIGVPEPLSVFVDTYKTGTIPDKDILVLIKEAFDFRPGMMAINLDLKRGGNFRFQK 364
Query: 680 TSTYGHFGR--AGFPWEIPKPL 739
T+ YGHFGR F WE+ KPL
Sbjct: 365 TAAYGHFGRDDPDFTWEVVKPL 386
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 94.3 bits (224), Expect = 8e-20
Identities = 44/78 (56%), Positives = 51/78 (65%)
Frame = +3
Query: 255 PAQYLDERTVIHINPCGLFIIGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVD 434
P +YLDE+T+ H+NP G F+IGGP DAGLTGRKII+DTY KD TKVD
Sbjct: 220 PEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVD 279
Query: 435 RSAAYAARWVAKSLVKAG 488
RS AY R AKS+V G
Sbjct: 280 RSGAYIVRQAAKSVVANG 297
Score = 91.5 bits (217), Expect = 6e-19
Identities = 47/97 (48%), Positives = 61/97 (62%)
Frame = +1
Query: 4 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSL 183
E MPL+ VLA KL ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S
Sbjct: 136 ELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLIST 195
Query: 184 QHSEKITLETLRDEIREKVIKEVILHNILMREL*FTL 294
QH E +T + + +++E VIK VI L + F L
Sbjct: 196 QHDETVTNDEIARDLKEHVIKPVIPEKYLDEKTIFHL 232
Score = 68.1 bits (159), Expect = 6e-12
Identities = 36/82 (43%), Positives = 51/82 (62%), Gaps = 5/82 (6%)
Frame = +2
Query: 509 QVAYAISVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA---PIYQR 679
QV+YAI V EPLS+ V Y T +++L IV+++FD RPG + L+L+ + +
Sbjct: 305 QVSYAIGVPEPLSVFVDTYETGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLK 364
Query: 680 TSTYGHFGR--AGFPWEIPKPL 739
T+ YGHFGR F WE+ KPL
Sbjct: 365 TAAYGHFGRDDPDFTWEVVKPL 386
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 94.3 bits (224), Expect = 8e-20
Identities = 44/78 (56%), Positives = 51/78 (65%)
Frame = +3
Query: 255 PAQYLDERTVIHINPCGLFIIGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVD 434
P +YLDE+T+ H+NP G F+IGGP DAGLTGRKII+DTY KD TKVD
Sbjct: 220 PEKYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVD 279
Query: 435 RSAAYAARWVAKSLVKAG 488
RS AY R AKS+V G
Sbjct: 280 RSGAYIVRQAAKSVVANG 297
Score = 91.5 bits (217), Expect = 6e-19
Identities = 47/97 (48%), Positives = 61/97 (62%)
Frame = +1
Query: 4 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSL 183
E MPL+ VLA KL ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S
Sbjct: 136 ELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLIST 195
Query: 184 QHSEKITLETLRDEIREKVIKEVILHNILMREL*FTL 294
QH E +T + + +++E VIK VI L + F L
Sbjct: 196 QHDETVTNDEIARDLKEHVIKPVIPEKYLDEKTIFHL 232
Score = 68.1 bits (159), Expect = 6e-12
Identities = 36/82 (43%), Positives = 51/82 (62%), Gaps = 5/82 (6%)
Frame = +2
Query: 509 QVAYAISVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA---PIYQR 679
QV+YAI V EPLS+ V Y T +++L IV+++FD RPG + L+L+ + +
Sbjct: 305 QVSYAIGVPEPLSVFVDTYETGLIPDKEILKIVKESFDFRPGMMTINLDLKRGGNGRFLK 364
Query: 680 TSTYGHFGR--AGFPWEIPKPL 739
T+ YGHFGR F WE+ KPL
Sbjct: 365 TAAYGHFGRDDPDFTWEVVKPL 386
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 93.1 bits (221), Expect = 2e-19
Identities = 43/78 (55%), Positives = 51/78 (65%)
Frame = +3
Query: 255 PAQYLDERTVIHINPCGLFIIGGPQSDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFTKVD 434
P +YLD++T+ H+NP G F+IGGP DAGLTGRKII+DTY KD TKVD
Sbjct: 220 PEKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVD 279
Query: 435 RSAAYAARWVAKSLVKAG 488
RS AY R AKS+V G
Sbjct: 280 RSGAYIVRQAAKSVVANG 297
Score = 91.1 bits (216), Expect = 7e-19
Identities = 42/84 (50%), Positives = 57/84 (67%)
Frame = +1
Query: 4 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSL 183
E MPL+ VLA K+ ++ E+R+NG W RPD KTQVT EY GA VP RVHTV++S
Sbjct: 136 ELMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLIST 195
Query: 184 QHSEKITLETLRDEIREKVIKEVI 255
QH E +T + + +++E VIK +I
Sbjct: 196 QHDETVTNDEIARDLKEHVIKPII 219
Score = 73.7 bits (173), Expect = 1e-13
Identities = 38/82 (46%), Positives = 52/82 (63%), Gaps = 5/82 (6%)
Frame = +2
Query: 509 QVAYAISVAEPLSITVFDYGTSHKTQQQLLSIVQKNFDLRPGKIVKELNLRA---PIYQR 679
QV+YAI V EPLS+ V YGT +++L IV++ FD RPG + L+L+ +Q+
Sbjct: 305 QVSYAIGVPEPLSVFVDTYGTGLIPDKEILKIVKETFDFRPGMMTINLDLKRGGNGRFQK 364
Query: 680 TSTYGHFGR--AGFPWEIPKPL 739
T+ YGHFGR F WE+ KPL
Sbjct: 365 TAAYGHFGRDDPDFTWEVVKPL 386
>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
PIR2:A28490
Length = 725
Score = 28.3 bits (60), Expect = 6.0
Identities = 16/39 (41%), Positives = 18/39 (46%)
Frame = +2
Query: 461 GRQVARQGGLVPVLHGQVAYAISVAEPLSITVFDYGTSH 577
GRQ A+ G VP G V PLS + YG SH
Sbjct: 434 GRQGAQSAGPVPPPSGPVPSPAFGGPPLSQVSYGYGQSH 472
>At1g58230.1 68414.m06618 WD-40 repeat family protein / beige-related
contains Pfam PF00400: WD domain, G-beta repeat; similar
to Lipopolysaccharide-responsive and beige-like anchor
protein (CDC4-like protein) (Beige-like protein)
(SP:P50851) [Homo sapiens}
Length = 1280
Score = 28.3 bits (60), Expect = 6.0
Identities = 13/45 (28%), Positives = 26/45 (57%)
Frame = +2
Query: 380 VGGARRGRFLWQGLHQGRPLRSIRRPMGRQVARQGGLVPVLHGQV 514
+ G++ G ++ L +GR +RS++ P G V++ L HG++
Sbjct: 1127 ISGSKDGTCVFHTLREGRYIRSLKHPSGSAVSK---LAASHHGRI 1168
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,047,213
Number of Sequences: 28952
Number of extensions: 351409
Number of successful extensions: 1019
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1013
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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