BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0325.Seq (565 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g30010.1 68415.m03651 expressed protein 31 0.53 At5g67300.1 68418.m08486 myb family transcription factor contain... 28 3.7 At5g53520.1 68418.m06651 oligopeptide transporter OPT family pro... 28 3.7 At1g10010.1 68414.m01129 amino acid permease, putative similar t... 28 3.7 At5g57670.1 68418.m07207 protein kinase family protein contains ... 28 5.0 At3g50950.2 68416.m05579 disease resistance protein (CC-NBS-LRR ... 27 6.5 At3g50950.1 68416.m05578 disease resistance protein (CC-NBS-LRR ... 27 6.5 At1g33170.1 68414.m04096 dehydration-responsive family protein s... 27 6.5 At5g15110.1 68418.m01770 pectate lyase family protein similar to... 27 8.7 At4g11420.1 68417.m01840 eukaryotic translation initiation facto... 27 8.7 At4g01525.1 68417.m00197 expressed protein 27 8.7 At3g43790.1 68416.m04678 transporter-related low similarity to S... 27 8.7 At3g19100.1 68416.m02427 calcium-dependent protein kinase, putat... 27 8.7 At2g33080.1 68415.m04056 leucine-rich repeat family protein cont... 27 8.7 At1g55050.1 68414.m06288 expressed protein ; expression supporte... 27 8.7 >At2g30010.1 68415.m03651 expressed protein Length = 398 Score = 31.1 bits (67), Expect = 0.53 Identities = 20/50 (40%), Positives = 26/50 (52%) Frame = -3 Query: 215 SVSPTHTVCAPSSWASRVKSTGIKVVGR*CCGQLNSIPPEIHGPSSPTSA 66 SVSPTH P+ W SR K++ I G+ C GQ G + PTS+ Sbjct: 276 SVSPTHY--NPNEWTSRSKTSTITQGGKSCYGQTT----PFSGTTYPTSS 319 >At5g67300.1 68418.m08486 myb family transcription factor contains PFAM profile: myb DNA binding domain PF00249 Length = 305 Score = 28.3 bits (60), Expect = 3.7 Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 2/39 (5%) Frame = +3 Query: 411 KRAVSAF--PIATGTYYKVDYSAGVDISRYKNVPVQPHI 521 KR+VSA P+ TG Y G D+S +P+ P + Sbjct: 125 KRSVSAGSPPVVTGLYMSPGSPTGSDVSDSSTIPILPSV 163 >At5g53520.1 68418.m06651 oligopeptide transporter OPT family protein similar to SP|P40900 Sexual differentiation process protein isp4 {Schizosaccharomyces pombe}, oligopeptide transporter Opt1p [Candida albicans] GI:2367386; contains Pfam profile PF03169: OPT oligopeptide transporter protein Length = 733 Score = 28.3 bits (60), Expect = 3.7 Identities = 13/45 (28%), Positives = 23/45 (51%), Gaps = 2/45 (4%) Frame = +2 Query: 29 SAILFITMK--SLNLRWWGCWGRGFPVGLSLTGRNIIARPPLCPL 157 S +LF+ ++ + + WWG G G PV T + ++ CP+ Sbjct: 690 SVLLFLALQRSEIAIDWWGNSGEGCPVAKCPTAKGVVVHG--CPV 732 >At1g10010.1 68414.m01129 amino acid permease, putative similar to amino acid permease I GI:22641 from [Arabidopsis thaliana]; GC splice site at position 1256 is predicted from alignment and not confirmed experimentally Length = 475 Score = 28.3 bits (60), Expect = 3.7 Identities = 21/61 (34%), Positives = 30/61 (49%) Frame = +1 Query: 187 AQTVWVGETEPMHGLQVMTGFTLALTGRRWKSPAASITGTPRRVISCGGPTRQ*KGGKGI 366 AQ WV T + ++T +T L ++SP SITGT R + G R GGK + Sbjct: 56 AQLGWVAGTTVLVAFAIITYYTSTLLADCYRSP-DSITGT--RNYNYMGVVRSYLGGKKV 112 Query: 367 R 369 + Sbjct: 113 Q 113 >At5g57670.1 68418.m07207 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 416 Score = 27.9 bits (59), Expect = 5.0 Identities = 14/36 (38%), Positives = 19/36 (52%) Frame = +3 Query: 273 LEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFP 380 LEI + N T +H L WA PA++ G + V P Sbjct: 297 LEIITGRRPVNPTQKHILLWAKPAMETGNTSELVDP 332 >At3g50950.2 68416.m05579 disease resistance protein (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists, suggestive of a disease resistance protein. Length = 852 Score = 27.5 bits (58), Expect = 6.5 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +3 Query: 207 RNGADAWFTGDDRFHPRPDRAALEIASRVYNG 302 RNG A +G+D F +R +E+ + Y+G Sbjct: 449 RNGRSATESGEDCFSGLTNRCLIEVVDKTYSG 480 >At3g50950.1 68416.m05578 disease resistance protein (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists, suggestive of a disease resistance protein. Length = 852 Score = 27.5 bits (58), Expect = 6.5 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +3 Query: 207 RNGADAWFTGDDRFHPRPDRAALEIASRVYNG 302 RNG A +G+D F +R +E+ + Y+G Sbjct: 449 RNGRSATESGEDCFSGLTNRCLIEVVDKTYSG 480 >At1g33170.1 68414.m04096 dehydration-responsive family protein similar to early-responsive to dehydration stress ERD3 protein [Arabidopsis thaliana] GI:15320410; contains Pfam profile PF03141: Putative methyltransferase Length = 639 Score = 27.5 bits (58), Expect = 6.5 Identities = 16/56 (28%), Positives = 22/56 (39%) Frame = -3 Query: 386 IRREDALMPFPPFHCRVGPPQEMTRRGVPVIDAAGDFQRRPVRARVKPVITCKPCI 219 ++R+ M F P Q RGVP I +R P AR + C C+ Sbjct: 250 LKRDIVAMSFAPRDTHEAQVQFALERGVPAIIGIMGSRRLPYPARAFDLAHCSRCL 305 >At5g15110.1 68418.m01770 pectate lyase family protein similar to pectate lyase P59 SP:P15722 from [Lycopersicon esculentum] Length = 472 Score = 27.1 bits (57), Expect = 8.7 Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 2/37 (5%) Frame = -2 Query: 510 GQAHFYSEKCPLQRSSPL--CSKCR*RWGKRRRPVCR 406 G+ + K P S+P+ C +CR W KRR+ + R Sbjct: 107 GKGKWSKLKGPCTASNPIDKCWRCRSDWAKRRKKLTR 143 >At4g11420.1 68417.m01840 eukaryotic translation initiation factor 3 subunit 10 / eIF-3 theta / eIF3a (TIF3A1) identical to eukaryotic translation initiation factor 3 subunit 10 (eIF-3 theta) (Eukaryotic translation initiation factor 3 large subunit) (eIF3a) (p114). [Arabidopsis thaliana] SWISS-PROT:Q9LD55 Length = 987 Score = 27.1 bits (57), Expect = 8.7 Identities = 15/61 (24%), Positives = 22/61 (36%) Frame = -2 Query: 438 RWGKRRRPVCRDQTPPLHPAGRRSDALPPLSLPGWPTTRNDAAWRSRYRRGWRFPAPPGQ 259 +W ++ V P RRS+ PP W + R A R+ PP + Sbjct: 899 KWKRQTTEVSGPSAPTSSETDRRSNRGPPPGDDHWGSNRGAAQNTDRWTSNRERSGPPAE 958 Query: 258 G 256 G Sbjct: 959 G 959 >At4g01525.1 68417.m00197 expressed protein Length = 142 Score = 27.1 bits (57), Expect = 8.7 Identities = 12/31 (38%), Positives = 18/31 (58%) Frame = +2 Query: 467 LRWSGHFSL*KCACPTSYMAEKSQYDFVGAW 559 L W G FSL +C+C + + +S YD + W Sbjct: 28 LDWCG-FSLLRCSCGSDVLEVESCYDSLRLW 57 >At3g43790.1 68416.m04678 transporter-related low similarity to SP|P39843 Multidrug resistance protein 2 (Multidrug-efflux transporter 2) {Bacillus subtilis}; contains Pfam profile PF00083: major facilitator superfamily protein Length = 478 Score = 27.1 bits (57), Expect = 8.7 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = +1 Query: 85 GPWISGGIEFNWPQHHRPTTFMPVD 159 GP GG+ F+W Q + TF+P D Sbjct: 428 GP-AGGGVLFSWAQKRQDATFLPGD 451 >At3g19100.1 68416.m02427 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium/calmodulin-dependent protein kinase CaMK3 [Nicotiana tabacum] gi|16904226|gb|AAL30820; contains protein kinase domain, Pfam:PF00069; contains serine/threonine protein kinase domain, INTERPRO:IPR002290 Length = 599 Score = 27.1 bits (57), Expect = 8.7 Identities = 14/30 (46%), Positives = 17/30 (56%) Frame = +3 Query: 120 AATSSPDHLYAR*FHPRSP*RRCTDGVGRR 209 + TS+P AR FHP SP R D + RR Sbjct: 76 SVTSTPLRQLARAFHPPSPARHIRDVLRRR 105 >At2g33080.1 68415.m04056 leucine-rich repeat family protein contains leucine rich-repeat domain Pfam:PF00560, INTERPRO:IPR001611; contains similarity to Cf-2.2 [Lycopersicon pimpinellifolium] gi|1184077|gb|AAC15780 Length = 740 Score = 27.1 bits (57), Expect = 8.7 Identities = 10/34 (29%), Positives = 18/34 (52%) Frame = +3 Query: 258 PDRAALEIASRVYNGNATPRHFLWWANPAVKGGE 359 P+ EIA ++ G +PR+F+ W ++ E Sbjct: 529 PELRIFEIADNMFTGTLSPRYFVNWKTSSLTVNE 562 >At1g55050.1 68414.m06288 expressed protein ; expression supported by MPSS Length = 914 Score = 27.1 bits (57), Expect = 8.7 Identities = 11/29 (37%), Positives = 14/29 (48%) Frame = -2 Query: 318 DAAWRSRYRRGWRFPAPPGQGEGETCHHL 232 DA W RGWR P QG ++ H+ Sbjct: 321 DAVWPRLLHRGWRSELPKDQGYIKSKEHI 349 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,880,485 Number of Sequences: 28952 Number of extensions: 369458 Number of successful extensions: 1033 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 994 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1032 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1082538160 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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