Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0311.Seq
(472 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R... 95 2e-20
At4g28470.1 68417.m04073 26S proteasome regulatory subunit, puta... 89 1e-18
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 28 2.8
At5g46270.1 68418.m05696 disease resistance protein (TIR-NBS-LRR... 28 3.7
At3g01760.1 68416.m00114 lysine and histidine specific transport... 28 3.7
At2g36340.1 68415.m04460 DNA-binding storekeeper protein-related... 27 4.8
At2g32640.2 68415.m03985 expressed protein 27 4.8
At2g32640.1 68415.m03984 expressed protein 27 4.8
At5g53840.1 68418.m06690 F-box family protein (FBL13) contains F... 27 6.4
At5g22370.1 68418.m02610 ATP-binding family protein contains Pfa... 27 6.4
At5g03760.1 68418.m00339 glycosyl transferase family 2 protein s... 27 6.4
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 27 6.4
At4g31160.1 68417.m04423 transducin family protein / WD-40 repea... 27 8.5
At2g32415.1 68415.m03961 3'-5' exonuclease domain-containing pro... 27 8.5
At1g75420.1 68414.m08761 glycosyl transferase family 1 protein c... 27 8.5
>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
(RPN1) contains an APC-complex (cyclosome) and
proteasome component repeat ( PS50248)
Length = 891
Score = 95.1 bits (226), Expect = 2e-20
Identities = 46/71 (64%), Positives = 57/71 (80%)
Frame = -2
Query: 471 LLNQPALAGXLVVLTAFLDCKTIILGKSHYLLYVLATAMQPRWLVTLDENLQPLNVSVRV 292
LL+ ALAG + +L A LD K IILGK HY+LY L AMQPR ++T+DENL+PL+V VRV
Sbjct: 769 LLSPTALAGIVTLLHACLDMKPIILGKYHYVLYFLVLAMQPRMMLTVDENLKPLSVPVRV 828
Query: 291 GQAVDVIGKAG 259
GQAVDV+G+AG
Sbjct: 829 GQAVDVVGQAG 839
Score = 56.0 bits (129), Expect = 1e-08
Identities = 24/29 (82%), Positives = 28/29 (96%)
Frame = -3
Query: 203 GERAELATDEYIPLSPIMEGFVILKKNED 117
GERAELATD+YIPLSPI+EGF+ILK+N D
Sbjct: 859 GERAELATDKYIPLSPILEGFIILKENPD 887
Score = 27.5 bits (58), Expect = 4.8
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = -1
Query: 253 KTIAGSHTHTTPVLLSS 203
KTI G TH+TPVLL++
Sbjct: 842 KTITGFQTHSTPVLLAA 858
>At4g28470.1 68417.m04073 26S proteasome regulatory subunit, putative
contains Pfam domain PF01851: Proteasome/cyclosome repeat
Length = 1103
Score = 89.0 bits (211), Expect = 1e-18
Identities = 41/66 (62%), Positives = 55/66 (83%)
Frame = -2
Query: 456 ALAGXLVVLTAFLDCKTIILGKSHYLLYVLATAMQPRWLVTLDENLQPLNVSVRVGQAVD 277
ALAG + +L A LD K+IILGK HY+LY L AMQPR ++T+D++L+P++V VRVGQAVD
Sbjct: 877 ALAGIVTLLHACLDMKSIILGKYHYVLYFLVLAMQPRMMLTVDQSLKPISVPVRVGQAVD 936
Query: 276 VIGKAG 259
V+G+AG
Sbjct: 937 VVGQAG 942
Score = 28.3 bits (60), Expect = 2.8
Identities = 12/19 (63%), Positives = 17/19 (89%), Gaps = 1/19 (5%)
Frame = -3
Query: 203 GERAELATDEY-IPLSPIM 150
GERAELAT++Y +PL P++
Sbjct: 962 GERAELATEKYVVPLIPLL 980
Score = 27.5 bits (58), Expect = 4.8
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = -1
Query: 253 KTIAGSHTHTTPVLLSS 203
KTI G TH+TPVLL++
Sbjct: 945 KTITGFQTHSTPVLLAA 961
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 28.3 bits (60), Expect = 2.8
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +1
Query: 127 FLRITNPSIIGESGMYSSVASSARSPKTAAPASCE 231
F+ I N +I SGM ++ + SA P +AAP E
Sbjct: 904 FIEILNSMLIIGSGMEATTSKSADVPTSAAPVPME 938
>At5g46270.1 68418.m05696 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1145
Score = 27.9 bits (59), Expect = 3.7
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +1
Query: 235 GNRLLSSCTGFSDNIHSLSHTHADVQGLKIFVEGDQPAG 351
G RLL C+ + + + +H + VQGL +E ++ +G
Sbjct: 1074 GIRLLEDCSSVENRLVNPTHAYTPVQGLVNEIEHNEDSG 1112
>At3g01760.1 68416.m00114 lysine and histidine specific transporter,
putative similar to lysine and histidine specific
transporter [Arabidopsis thaliana] GI:2576361; contains
Pfam profile PF01490: Transmembrane amino acid
transporter protein
Length = 479
Score = 27.9 bits (59), Expect = 3.7
Identities = 18/69 (26%), Positives = 29/69 (42%)
Frame = -3
Query: 353 SPAGWSPSTKIFSP*TSACVWDRLWMLSEKPVHEDNSRFPHSHDAGAAVFGERAELATDE 174
S GW P + ++ LW + E + RF H+ G A FG++ L
Sbjct: 84 SELGWGPGVVVLILSWVITLYT-LWQMIEMHEMFEGQRFDRYHELGQAAFGKKLGLYI-- 140
Query: 173 YIPLSPIME 147
+PL ++E
Sbjct: 141 IVPLQLLVE 149
>At2g36340.1 68415.m04460 DNA-binding storekeeper protein-related
contains similarity to storekeeper protein [Solanum
tuberosum] gi|14268476|emb|CAC39398; contains PF04504:
Protein of unknown function, DUF573
Length = 414
Score = 27.5 bits (58), Expect = 4.8
Identities = 15/30 (50%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -1
Query: 271 RKSRYTKTIAGSHTHTTPV-LLSSASEQNW 185
RK T T + T TTP+ L SSAS+ NW
Sbjct: 34 RKQLRTTTTRTTTTRTTPLSLSSSASKMNW 63
>At2g32640.2 68415.m03985 expressed protein
Length = 385
Score = 27.5 bits (58), Expect = 4.8
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 340 QPAGLHRSRQYVQQVVGFAEDYGL 411
QP G ++Q V +V GF++DY +
Sbjct: 81 QPTGPQETQQIVSRVSGFSQDYSM 104
>At2g32640.1 68415.m03984 expressed protein
Length = 585
Score = 27.5 bits (58), Expect = 4.8
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 340 QPAGLHRSRQYVQQVVGFAEDYGL 411
QP G ++Q V +V GF++DY +
Sbjct: 81 QPTGPQETQQIVSRVSGFSQDYSM 104
>At5g53840.1 68418.m06690 F-box family protein (FBL13) contains
F-box domain PF:00646
Length = 444
Score = 27.1 bits (57), Expect = 6.4
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -3
Query: 191 ELATDEYIPLSPIMEGFVILKKNEDSV 111
E D+ I SP++E I+K +ED+V
Sbjct: 177 ETTLDKIISCSPVLEELTIVKSSEDNV 203
>At5g22370.1 68418.m02610 ATP-binding family protein contains Pfam
domain, PF03029: Conserved hypothetical ATP binding
protein
Length = 291
Score = 27.1 bits (57), Expect = 6.4
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 120 LVFLENHESLHYRRERYVLVSRQFCSLAEDSS 215
L +LE+H S R +Y ++++ CS+ ED S
Sbjct: 197 LSYLEHHLSQDPRSAKYRKLTKELCSVIEDYS 228
>At5g03760.1 68418.m00339 glycosyl transferase family 2 protein
similar to beta-(1-3)-glucosyl transferase GB:AAC62210
GI:3687658 from [Bradyrhizobium japonicum], cellulose
synthase from Agrobacterium tumeficiens [gi:710492] and
Agrobacterium radiobacter [gi:710493]; contains Pfam
glycosyl transferase, group 2 family protein domain
PF00535
Length = 533
Score = 27.1 bits (57), Expect = 6.4
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -2
Query: 423 FLDCKTIILGKSHYLLYVLATAM 355
F+ C GK+HY LY+ A A+
Sbjct: 494 FVGCYDAFFGKNHYYLYLFAQAI 516
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 27.1 bits (57), Expect = 6.4
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 127 FLRITNPSIIGESGMYSSVASSARSPKTAAPASCE 231
F+ I N +I SGM ++ + SA P AAP E
Sbjct: 657 FIEILNSILIIGSGMEATTSKSADVPTDAAPVPME 691
>At4g31160.1 68417.m04423 transducin family protein / WD-40 repeat
family protein contains 2 WD-40 repeats (PF00400);
KIAA0800 protein, Homo sapiens GI:3882321 EMBL:AB018343
Length = 1846
Score = 26.6 bits (56), Expect = 8.5
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +3
Query: 282 QPVPHAR*RSRAEDFRRG*PASGAASQSPIRTASSGI 392
QPVP + D G ++G A Q+P+ ASSG+
Sbjct: 891 QPVPGQATTRPSTDVAVGTQSTGNAPQTPVAPASSGL 927
>At2g32415.1 68415.m03961 3'-5' exonuclease domain-containing
protein similar to SP|Q12149 Exosome complex exonuclease
RRP6 (EC 3.1.13.-) (Ribosomal RNA processing protein 6)
{Saccharomyces cerevisiae}
Length = 880
Score = 26.6 bits (56), Expect = 8.5
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 99 NRGHDAILVFLENHESLHYRRERY 170
+R HD +L+ ++ HE H ERY
Sbjct: 601 HRSHDIVLLCVDCHEVAHAAAERY 624
>At1g75420.1 68414.m08761 glycosyl transferase family 1 protein
contains Pfam glycosyl transferase, group 1 family
protein domain PF00534
Length = 463
Score = 26.6 bits (56), Expect = 8.5
Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 3/64 (4%)
Frame = -3
Query: 290 DRLWMLSEKPVHEDNSRFPHSH---DAGAAVFGERAELATDEYIPLSPIMEGFVILKKNE 120
D +W+ ++KP+ +D + H D G V + + A D + I+ + K
Sbjct: 98 DVVWITNQKPLEDDEVVYSLEHKMLDRGVQVISAKGQKAVDTSLKADLIVLNTAVAGKWL 157
Query: 119 DSVM 108
D+V+
Sbjct: 158 DAVL 161
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,777,129
Number of Sequences: 28952
Number of extensions: 173566
Number of successful extensions: 500
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 489
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 500
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 801831960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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