BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0305.Seq (664 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g76965.1 68414.m08961 glycine-rich protein 30 1.2 At5g16980.1 68418.m01989 NADP-dependent oxidoreductase, putative... 30 1.6 At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containi... 30 1.6 At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 29 3.6 At5g42370.1 68418.m05159 expressed protein 29 3.6 At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identic... 29 3.6 At2g02800.2 68415.m00225 protein kinase (APK2b) identical to pro... 29 3.6 At2g02800.1 68415.m00224 protein kinase (APK2b) identical to pro... 29 3.6 At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative... 28 4.8 At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 4.8 At5g22390.1 68418.m02612 expressed protein 27 8.4 At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative... 27 8.4 >At1g76965.1 68414.m08961 glycine-rich protein Length = 158 Score = 30.3 bits (65), Expect = 1.2 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -1 Query: 454 PGTGCIRFPSKPDTPRSSEPILIPKLRIQFAD 359 PG + FP KP+ P P +P+L + F D Sbjct: 93 PGAAIVVFPKKPEEPVKVVPTPMPQLNLFFGD 124 >At5g16980.1 68418.m01989 NADP-dependent oxidoreductase, putative strong similarity to probable NADP-dependent oxidoreductase (zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430], Arabidopsis thaliana Length = 239 Score = 29.9 bits (64), Expect = 1.6 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = -2 Query: 585 RVRIQSET*DDFRECHIKYIQFLRPHI 505 R+RIQ DF + + K+++FL PHI Sbjct: 172 RIRIQGFVVSDFYDEYSKFLEFLHPHI 198 >At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containing protein Length = 964 Score = 29.9 bits (64), Expect = 1.6 Identities = 23/69 (33%), Positives = 27/69 (39%), Gaps = 3/69 (4%) Frame = -2 Query: 408 GPQSQSLFRSYGSNLPTSLTYIILSTRGSS---PWRPAADMGTNQSDISTYIPHRIFKVR 238 G Q Y S P S +Y T GS+ P P A G S +S Y P R +V Sbjct: 519 GSQKSQYGLPYKSGSPNSHSYYGSPTFGSNMSYPGSPLAHHGMPNSLMSPYSPMRRGEVN 578 Query: 237 REYPDTAAN 211 YP N Sbjct: 579 MRYPAATRN 587 >At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to autophagy 7 [Arabidopsis thaliana] GI:19912147; contains Pfam profile PF00899: ThiF family Length = 697 Score = 28.7 bits (61), Expect = 3.6 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = -1 Query: 241 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 107 P ++G CG +V NH++LL +S+ L+ ++S +R + Sbjct: 42 PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86 >At5g42370.1 68418.m05159 expressed protein Length = 447 Score = 28.7 bits (61), Expect = 3.6 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -2 Query: 180 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSNTR 70 P Y + + Q+ +K +P PL + R L W TPS R Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTMR 322 >At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identical to low affinity calcium antiporter CAX2 (GI:1488267) [Arabidopsis thaliana]; Ca2+:Cation Antiporter (CaCA) Family member PMID:11500563 Length = 441 Score = 28.7 bits (61), Expect = 3.6 Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%) Frame = +1 Query: 43 SIIVPSSLKTSVRRGNPKWPEDAAERSGKSFLFC--LSVRVPWNPI 174 S++ SL TS + PK P+++ S K +FC L++ +P+ P+ Sbjct: 41 SLMEQGSLSTSFPQHTPKAPKNSVLNSIKIVIFCNKLNLLLPFGPL 86 >At2g02800.2 68415.m00225 protein kinase (APK2b) identical to protein kinase APK2b [Arabidopsis thaliana] gi|2852449|dbj|BAA24695 Length = 426 Score = 28.7 bits (61), Expect = 3.6 Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Frame = -1 Query: 463 STRPGTGCIRFPSKPDTPRSSEPILIPKLRIQFA-DFPYLH 344 ST+PGTG ++ D+PR S ++ K +++ D P LH Sbjct: 368 STKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSYDRPLLH 408 >At2g02800.1 68415.m00224 protein kinase (APK2b) identical to protein kinase APK2b [Arabidopsis thaliana] gi|2852449|dbj|BAA24695 Length = 426 Score = 28.7 bits (61), Expect = 3.6 Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Frame = -1 Query: 463 STRPGTGCIRFPSKPDTPRSSEPILIPKLRIQFA-DFPYLH 344 ST+PGTG ++ D+PR S ++ K +++ D P LH Sbjct: 368 STKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSYDRPLLH 408 >At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative (P1) identical to probable NADP-dependent oxidoreductase P1, zeta-crystallin homolog [SP|Q39172][gi:886428], Arabidopsis thaliana; similar to allyl alcohol dehydrogenase [Nicotiana tabacum] GI:6692816; contains Pfam profile PF00107: oxidoreductase, zinc-binding dehydrogenase family Length = 345 Score = 28.3 bits (60), Expect = 4.8 Identities = 11/27 (40%), Positives = 18/27 (66%) Frame = -2 Query: 585 RVRIQSET*DDFRECHIKYIQFLRPHI 505 R+RIQ DF + + K+++F+ PHI Sbjct: 278 RIRIQGFVVSDFYDKYSKFLEFVLPHI 304 >At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140 [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis thaliana] Length = 926 Score = 28.3 bits (60), Expect = 4.8 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -3 Query: 236 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 138 +S + P R ++PYLPS G RTLR+K Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247 >At5g22390.1 68418.m02612 expressed protein Length = 202 Score = 27.5 bits (58), Expect = 8.4 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = +1 Query: 115 ERSGKSFLFCLSVRVPWNPIEG 180 + S KSFL LS PWNP +G Sbjct: 17 DNSPKSFLDTLSSSSPWNPSKG 38 >At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative strong similarity to probable NADP-dependent oxidoreductase (zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430], Arabidopsis thaliana Length = 345 Score = 27.5 bits (58), Expect = 8.4 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = -2 Query: 585 RVRIQSET*DDFRECHIKYIQFLRPHI 505 R+RIQ DF E + K++ F+ PHI Sbjct: 278 RIRIQGFAVFDFYEKYSKFLDFVLPHI 304 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,573,638 Number of Sequences: 28952 Number of extensions: 341901 Number of successful extensions: 1020 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 984 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1020 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1393347168 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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