Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0299.Seq
(748 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 209 3e-55
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 116 4e-27
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 113 3e-26
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 96 5e-21
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.70
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 29 0.93
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.1
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.8
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.8
SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc... 26 5.0
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 5.0
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 26 6.6
SPAC2E1P5.05 |||U3 snoRNP-associated protein Rrp9 |Schizosacchar... 26 6.6
SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyce... 25 8.7
SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein Pop2|Schizosacchar... 25 8.7
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 8.7
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 209 bits (511), Expect = 3e-55
Identities = 97/136 (71%), Positives = 112/136 (82%)
Frame = +3
Query: 252 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 431
LEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 432 DCLQGFQLTHFPRWRHRVRYGHPPHLKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATL 611
D LQGFQLTH G KIREEYPDR+M T+SV P+PK SDTVVEPYNATL
Sbjct: 128 DALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATL 187
Query: 612 SVHQLVENTDETYCID 659
S+HQLVEN+DET+CID
Sbjct: 188 SMHQLVENSDETFCID 203
Score = 123 bits (296), Expect = 3e-29
Identities = 51/70 (72%), Positives = 60/70 (85%)
Frame = +1
Query: 52 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 231
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 232 PRAILVDLSP 261
PRA+LVDL P
Sbjct: 61 PRAVLVDLEP 70
Score = 37.9 bits (84), Expect = 0.002
Identities = 17/27 (62%), Positives = 20/27 (74%)
Frame = +1
Query: 661 NEALYDICFRTLKLSTPTYGDLNHLVS 741
NEAL I TLK+ +P+Y DLNHLVS
Sbjct: 204 NEALSSIFANTLKIKSPSYDDLNHLVS 230
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 116 bits (279), Expect = 4e-27
Identities = 54/136 (39%), Positives = 85/136 (62%)
Frame = +3
Query: 252 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 431
LEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++C
Sbjct: 74 LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNC 133
Query: 432 DCLQGFQLTHFPRWRHRVRYGHPPHLKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATL 611
LQGF + H G ++ EY + +SV P+P+VS +VVEPYN+ L
Sbjct: 134 SGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVL 193
Query: 612 SVHQLVENTDETYCID 659
+ H ++N+D T+ +D
Sbjct: 194 TTHATLDNSDCTFMVD 209
Score = 58.4 bits (135), Expect = 1e-09
Identities = 29/76 (38%), Positives = 41/76 (53%), Gaps = 6/76 (7%)
Frame = +1
Query: 52 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 213
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 214 SGGKYVPRAILVDLSP 261
GK+VPR+I VDL P
Sbjct: 61 GQGKFVPRSIYVDLEP 76
Score = 37.1 bits (82), Expect = 0.003
Identities = 15/27 (55%), Positives = 19/27 (70%)
Frame = +1
Query: 661 NEALYDICFRTLKLSTPTYGDLNHLVS 741
NEA YDIC R L + PTY +LN L++
Sbjct: 210 NEACYDICRRNLDIERPTYENLNRLIA 236
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 113 bits (271), Expect = 3e-26
Identities = 53/136 (38%), Positives = 82/136 (60%)
Frame = +3
Query: 252 LEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 431
LEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++C
Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129
Query: 432 DCLQGFQLTHFPRWRHRVRYGHPPHLKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATL 611
LQGF + H +G ++ EY + +SV P+P+VS +VVEPYN+ L
Sbjct: 130 SGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVL 189
Query: 612 SVHQLVENTDETYCID 659
+ H ++ D T+ +D
Sbjct: 190 TTHATLDLADCTFMVD 205
Score = 64.5 bits (150), Expect = 2e-11
Identities = 31/72 (43%), Positives = 42/72 (58%), Gaps = 2/72 (2%)
Frame = +1
Query: 52 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 225
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 226 YVPRAILVDLSP 261
YVPR+I VDL P
Sbjct: 61 YVPRSIYVDLEP 72
Score = 34.3 bits (75), Expect = 0.019
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +1
Query: 661 NEALYDICFRTLKLSTPTYGDLNHLVS 741
NE+ YDIC R L + P+Y +LN L++
Sbjct: 206 NESCYDICRRNLDIERPSYENLNRLIA 232
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 95.9 bits (228), Expect = 5e-21
Identities = 48/147 (32%), Positives = 84/147 (57%), Gaps = 3/147 (2%)
Frame = +3
Query: 252 LEPGTMDSVRSGPFGQIFRPDNFVFGQSG--AGNNWAKGHYTEGAELVDSVLDVVRKESE 425
LEP ++++ S +G ++ P+N + ++G AGNNWA G Y+ + + ++D++ +E++
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 426 SCDCLQGFQLTHFPRWRHRVRYGHPPHLKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYN 602
D L+GF L H G ++ + YP +I+ TYSV P S VSD VV+PYN
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187
Query: 603 ATLSVHQLVENTDETYCIDKRGSIRHL 683
+ L++ +L N D +D ++ H+
Sbjct: 188 SLLALKRLTLNADSVVVLD-NAALAHI 213
Score = 78.6 bits (185), Expect = 9e-16
Identities = 33/69 (47%), Positives = 49/69 (71%)
Frame = +1
Query: 55 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 234
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 235 RAILVDLSP 261
RAIL+DL P
Sbjct: 63 RAILIDLEP 71
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.1 bits (62), Expect = 0.70
Identities = 16/27 (59%), Positives = 17/27 (62%)
Frame = +2
Query: 437 PTGLPTYTFPSVAAPGPVWAPSSSKDP 517
P+G P PSVAAP PV APSS P
Sbjct: 1155 PSGAPPVPKPSVAAP-PVPAPSSGIPP 1180
Score = 27.1 bits (57), Expect = 2.8
Identities = 15/31 (48%), Positives = 18/31 (58%)
Frame = +2
Query: 437 PTGLPTYTFPSVAAPGPVWAPSSSKDP*RVP 529
P+G+P PSVAAP PV PS + P P
Sbjct: 1088 PSGIPPVPKPSVAAP-PVPKPSVAVPPVPAP 1117
Score = 27.1 bits (57), Expect = 2.8
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = +2
Query: 434 LPTGLPTYTFPSVAAPGPVWAPSSS 508
+P+G P PSVAAP PV APS +
Sbjct: 1135 VPSGAPPVPKPSVAAP-PVPAPSGA 1158
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 28.7 bits (61), Expect = 0.93
Identities = 19/49 (38%), Positives = 25/49 (51%), Gaps = 5/49 (10%)
Frame = +2
Query: 437 PTGL---PTYTFPSVAAP--GPVWAPSSSKDP*RVPRQNHEHILSSPLA 568
P GL P Y PS +P GPV +PSS K P + +H L P++
Sbjct: 57 PLGLSFSPGYISPSSQSPHHGPVRSPSSRKPLPASPSRTRDHSLRVPVS 105
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.1
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 354 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 443
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.8
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 91 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 192
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.8
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 285 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 386
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 849
Score = 26.2 bits (55), Expect = 5.0
Identities = 17/68 (25%), Positives = 28/68 (41%)
Frame = +3
Query: 453 LTHFPRWRHRVRYGHPPHLKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVE 632
L HFP+WR V L + Y + + + + S+ Y + HQL++
Sbjct: 377 LIHFPKWRGHVVLIQITSLPVGNNYSNEELKKTENLVAQINSEFGSLDYTPVIHFHQLLD 436
Query: 633 NTDETYCI 656
DE Y +
Sbjct: 437 -PDEYYAL 443
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 5.0
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 35 KITYTK*GKSFIYRPANVV 91
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1116
Score = 25.8 bits (54), Expect = 6.6
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +2
Query: 485 PVWAPSSSKDP*RVPRQNHEHILSSPLAQSIRHCRRTIQCDSLSSPAS*KHRR 643
P WA + +VP++NH H + S L+ + ++ + LSSP + ++ R
Sbjct: 628 PFWAIQPKQSSSQVPKENH-HNIQSKLSIN-TEAATDLKANELSSPKTPEYCR 678
>SPAC2E1P5.05 |||U3 snoRNP-associated protein Rrp9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 524
Score = 25.8 bits (54), Expect = 6.6
Identities = 15/57 (26%), Positives = 24/57 (42%)
Frame = -3
Query: 398 DRVNELSTLCVVSLGPVVASAGLSEDEVVRTEDLSERSRADRVHGAGLKSTRMARGT 228
D V E++ L + + G + + GL V+R E + H + M RGT
Sbjct: 206 DHVKEITCLAISNDGRWIVTGGLDHRIVIRDSVTLEPQHCWKHHRDAVMGLAMRRGT 262
>SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 428
Score = 25.4 bits (53), Expect = 8.7
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +2
Query: 467 SVAAPGPVWAPSSSKDP*RVPRQNHEHILSSPLAQS 574
S A P +PS+SK P R+N + SP+A +
Sbjct: 168 SAGAAAPTPSPSNSKSPPSENRKNRLQNVLSPIASN 203
>SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein
Pop2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 703
Score = 25.4 bits (53), Expect = 8.7
Identities = 18/61 (29%), Positives = 25/61 (40%)
Frame = +3
Query: 528 PDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDKRGSIRHLLPHSQTVH 707
P R++ + P V + TL V +L +NTD Y D SI + VH
Sbjct: 442 PSRLIRHGVEIVEPDQPYIVSGSRDHTLRVWKLPKNTDPPYLPDNTNSIDRWEKNPYFVH 501
Query: 708 T 710
T
Sbjct: 502 T 502
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 306 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 437
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,111,329
Number of Sequences: 5004
Number of extensions: 64650
Number of successful extensions: 199
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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