BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0288.Seq (656 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g20810.1 68417.m03020 transcription initiation factor IIE (TF... 41 6e-04 At1g03280.1 68414.m00306 transcription initiation factor IIE (TF... 39 0.003 At4g20340.1 68417.m02969 transcription initiation factor IIE (TF... 33 0.22 At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive eff... 31 0.51 At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive eff... 30 1.6 At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive eff... 28 4.8 At5g24740.1 68418.m02920 expressed protein 27 8.3 >At4g20810.1 68417.m03020 transcription initiation factor IIE (TFIIE) alpha subunit family protein / general transcription factor TFIIE family protein contains Pfam profile: PF02002 TFIIE alpha subunit Length = 416 Score = 41.1 bits (92), Expect = 6e-04 Identities = 27/93 (29%), Positives = 50/93 (53%), Gaps = 9/93 (9%) Frame = +3 Query: 261 LIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFI-QVRLKMETGL--DGKAQ- 428 L++D L R ++E+D+ + LK K LR + + +FI +V K ++ +G+ + Sbjct: 34 LVLDALTRRQWVREEDLAKELKLNTKQLRTILRYFEEQQFIMRVHRKEKSSATTNGRGED 93 Query: 429 --KVNYYF---INYKTFVNVVKYKLDLMRKRLK 512 KV+ Y ++Y +V++YKL M+K K Sbjct: 94 KVKVHMYSYCCLDYSQIYDVIRYKLHRMKKEFK 126 >At1g03280.1 68414.m00306 transcription initiation factor IIE (TFIIE) alpha subunit family protein / general transcription factor TFIIE family protein contains Pfam profile: PF02002 TFIIE alpha subunit Length = 479 Score = 39.1 bits (87), Expect = 0.003 Identities = 35/124 (28%), Positives = 61/124 (49%), Gaps = 19/124 (15%) Frame = +3 Query: 258 ALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQVRLKMETG--------- 410 A+++D L R ++E+D+ + L+ K LR I + + +K I + ET Sbjct: 51 AVVLDALARRQWVREEDLAKDLQLHAKQLRKIIRLFEEEKLIMRDHRKETAKGAKMYSAA 110 Query: 411 ----LDGKAQ-KV-----NYYFINYKTFVNVVKYKLDLMRKRLKQRNVTLQAEPVSSVQL 560 DG+A+ KV +Y ++Y +VV+++L M+KRLK + E ++VQ Sbjct: 111 VAATTDGRAEDKVKLHTHSYCCLDYAQICDVVRFRLHRMKKRLKD-----ELEDKNTVQE 165 Query: 561 VGRP 572 G P Sbjct: 166 YGCP 169 >At4g20340.1 68417.m02969 transcription initiation factor IIE (TFIIE) alpha subunit family protein / general transcription factor TFIIE family protein contains Pfam profile: PF02002 TFIIE alpha subunit Length = 527 Score = 32.7 bits (71), Expect = 0.22 Identities = 25/103 (24%), Positives = 48/103 (46%), Gaps = 19/103 (18%) Frame = +3 Query: 261 LIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQVRLKMETG---------- 410 +++D L R ++E+D+ + +K K LR I + KF+ + ET Sbjct: 52 IVLDALTRRQWVREEDLAKEVKRNAKELRKLIRHFEEQKFVMRYHRKETAKRAKMYSYAV 111 Query: 411 ---LDGKAQ------KVNYYFINYKTFVNVVKYKLDLMRKRLK 512 DG+A+ +Y ++Y ++V+YKL ++K+ K Sbjct: 112 GGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKFK 154 >At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive effector-related contains similarity to SEN1, a positive effector of tRNA-splicing endonuclease [Saccharomyces cerevisiae] gi|172574|gb|AAB63976 Length = 839 Score = 31.5 bits (68), Expect = 0.51 Identities = 24/105 (22%), Positives = 45/105 (42%) Frame = +3 Query: 210 KTASKISRQRLLHNRDALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQV 389 K ++ +L R A+++ + P M +++CE KF R + + + N + V Sbjct: 539 KECESVAALQLPGLRHAILIGDEFQLPAMVHNEMCEKAKFGRSLFERLVLLGHNKHLLDV 598 Query: 390 RLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRLKQRNV 524 + +M + K +Y K NV K + +KR Q N+ Sbjct: 599 QYRMHPSISRFPNK-EFYGGRIKDAENV---KESIYQKRFLQGNM 639 >At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive effector-related contains similarity to SEN1, a positive effector of tRNA-splicing endonuclease [Saccharomyces cerevisiae] gi|172574|gb|AAB63976 Length = 676 Score = 29.9 bits (64), Expect = 1.6 Identities = 23/105 (21%), Positives = 45/105 (42%) Frame = +3 Query: 210 KTASKISRQRLLHNRDALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQV 389 K ++ +L R A+++ + P M +++CE KF R + + + N + V Sbjct: 376 KECESVAALQLPGLRHAILIGDEFQLPAMVHNEMCEKAKFGRSLFERLVLLGHNKHLLDV 435 Query: 390 RLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRLKQRNV 524 + +M + K +Y K NV K + +KR + N+ Sbjct: 436 QYRMHPSISRFPNK-EFYGGRIKDAENV---KESIYQKRFLKGNM 476 >At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive effector-related contains similarity to SEN1, a positive effector of tRNA-splicing endonuclease [Saccharomyces cerevisiae] gi|172574|gb|AAB63976 Length = 638 Score = 28.3 bits (60), Expect = 4.8 Identities = 23/105 (21%), Positives = 43/105 (40%) Frame = +3 Query: 210 KTASKISRQRLLHNRDALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQV 389 K ++ +L R A+++ + P M +D CE KF R + + + + V Sbjct: 360 KECESVAALQLPGLRHAILIGDEFQLPAMVHNDQCEKAKFGRSLFERLVLLGHKKHLLDV 419 Query: 390 RLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRLKQRNV 524 + +M + K +Y K NV + + +KR Q N+ Sbjct: 420 QYRMHPSISRFPYK-EFYGGRIKDAANVQE---SIYQKRFLQGNM 460 >At5g24740.1 68418.m02920 expressed protein Length = 3306 Score = 27.5 bits (58), Expect = 8.3 Identities = 16/57 (28%), Positives = 32/57 (56%) Frame = +3 Query: 375 KFIQVRLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRLKQRNVTLQAEPV 545 +F+ +++++ L + + FI ++ FVN++ KL RL++ TL +EPV Sbjct: 1242 QFLHTKMRLKQELSVAPLEPSTSFIKFRIFVNLLTSKL----HRLRKAPGTLLSEPV 1294 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,988,814 Number of Sequences: 28952 Number of extensions: 214408 Number of successful extensions: 534 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 525 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 534 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1373722560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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