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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0288.Seq
         (656 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g20810.1 68417.m03020 transcription initiation factor IIE (TF...    41   6e-04
At1g03280.1 68414.m00306 transcription initiation factor IIE (TF...    39   0.003
At4g20340.1 68417.m02969 transcription initiation factor IIE (TF...    33   0.22 
At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive eff...    31   0.51 
At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive eff...    30   1.6  
At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive eff...    28   4.8  
At5g24740.1 68418.m02920 expressed protein                             27   8.3  

>At4g20810.1 68417.m03020 transcription initiation factor IIE
           (TFIIE) alpha subunit family protein / general
           transcription factor TFIIE family protein contains Pfam
           profile: PF02002 TFIIE alpha subunit
          Length = 416

 Score = 41.1 bits (92), Expect = 6e-04
 Identities = 27/93 (29%), Positives = 50/93 (53%), Gaps = 9/93 (9%)
 Frame = +3

Query: 261 LIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFI-QVRLKMETGL--DGKAQ- 428
           L++D L R   ++E+D+ + LK   K LR  +   +  +FI +V  K ++    +G+ + 
Sbjct: 34  LVLDALTRRQWVREEDLAKELKLNTKQLRTILRYFEEQQFIMRVHRKEKSSATTNGRGED 93

Query: 429 --KVNYYF---INYKTFVNVVKYKLDLMRKRLK 512
             KV+ Y    ++Y    +V++YKL  M+K  K
Sbjct: 94  KVKVHMYSYCCLDYSQIYDVIRYKLHRMKKEFK 126


>At1g03280.1 68414.m00306 transcription initiation factor IIE
           (TFIIE) alpha subunit family protein / general
           transcription factor TFIIE family protein contains Pfam
           profile: PF02002 TFIIE alpha subunit
          Length = 479

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 35/124 (28%), Positives = 61/124 (49%), Gaps = 19/124 (15%)
 Frame = +3

Query: 258 ALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQVRLKMETG--------- 410
           A+++D L R   ++E+D+ + L+   K LR  I + + +K I    + ET          
Sbjct: 51  AVVLDALARRQWVREEDLAKDLQLHAKQLRKIIRLFEEEKLIMRDHRKETAKGAKMYSAA 110

Query: 411 ----LDGKAQ-KV-----NYYFINYKTFVNVVKYKLDLMRKRLKQRNVTLQAEPVSSVQL 560
                DG+A+ KV     +Y  ++Y    +VV+++L  M+KRLK      + E  ++VQ 
Sbjct: 111 VAATTDGRAEDKVKLHTHSYCCLDYAQICDVVRFRLHRMKKRLKD-----ELEDKNTVQE 165

Query: 561 VGRP 572
            G P
Sbjct: 166 YGCP 169


>At4g20340.1 68417.m02969 transcription initiation factor IIE
           (TFIIE) alpha subunit family protein / general
           transcription factor TFIIE family protein contains Pfam
           profile: PF02002 TFIIE alpha subunit
          Length = 527

 Score = 32.7 bits (71), Expect = 0.22
 Identities = 25/103 (24%), Positives = 48/103 (46%), Gaps = 19/103 (18%)
 Frame = +3

Query: 261 LIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQVRLKMETG---------- 410
           +++D L R   ++E+D+ + +K   K LR  I   +  KF+    + ET           
Sbjct: 52  IVLDALTRRQWVREEDLAKEVKRNAKELRKLIRHFEEQKFVMRYHRKETAKRAKMYSYAV 111

Query: 411 ---LDGKAQ------KVNYYFINYKTFVNVVKYKLDLMRKRLK 512
               DG+A+        +Y  ++Y    ++V+YKL  ++K+ K
Sbjct: 112 GGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKFK 154


>At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive
           effector-related contains similarity to SEN1, a positive
           effector of tRNA-splicing endonuclease [Saccharomyces
           cerevisiae] gi|172574|gb|AAB63976
          Length = 839

 Score = 31.5 bits (68), Expect = 0.51
 Identities = 24/105 (22%), Positives = 45/105 (42%)
 Frame = +3

Query: 210 KTASKISRQRLLHNRDALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQV 389
           K    ++  +L   R A+++    + P M  +++CE  KF R +    + +  N   + V
Sbjct: 539 KECESVAALQLPGLRHAILIGDEFQLPAMVHNEMCEKAKFGRSLFERLVLLGHNKHLLDV 598

Query: 390 RLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRLKQRNV 524
           + +M   +     K  +Y    K   NV   K  + +KR  Q N+
Sbjct: 599 QYRMHPSISRFPNK-EFYGGRIKDAENV---KESIYQKRFLQGNM 639


>At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive
           effector-related contains similarity to SEN1, a positive
           effector of tRNA-splicing endonuclease [Saccharomyces
           cerevisiae] gi|172574|gb|AAB63976
          Length = 676

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 23/105 (21%), Positives = 45/105 (42%)
 Frame = +3

Query: 210 KTASKISRQRLLHNRDALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQV 389
           K    ++  +L   R A+++    + P M  +++CE  KF R +    + +  N   + V
Sbjct: 376 KECESVAALQLPGLRHAILIGDEFQLPAMVHNEMCEKAKFGRSLFERLVLLGHNKHLLDV 435

Query: 390 RLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRLKQRNV 524
           + +M   +     K  +Y    K   NV   K  + +KR  + N+
Sbjct: 436 QYRMHPSISRFPNK-EFYGGRIKDAENV---KESIYQKRFLKGNM 476


>At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive
           effector-related contains similarity to SEN1, a positive
           effector of tRNA-splicing endonuclease [Saccharomyces
           cerevisiae] gi|172574|gb|AAB63976
          Length = 638

 Score = 28.3 bits (60), Expect = 4.8
 Identities = 23/105 (21%), Positives = 43/105 (40%)
 Frame = +3

Query: 210 KTASKISRQRLLHNRDALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQV 389
           K    ++  +L   R A+++    + P M  +D CE  KF R +    + +      + V
Sbjct: 360 KECESVAALQLPGLRHAILIGDEFQLPAMVHNDQCEKAKFGRSLFERLVLLGHKKHLLDV 419

Query: 390 RLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRLKQRNV 524
           + +M   +     K  +Y    K   NV +    + +KR  Q N+
Sbjct: 420 QYRMHPSISRFPYK-EFYGGRIKDAANVQE---SIYQKRFLQGNM 460


>At5g24740.1 68418.m02920 expressed protein
          Length = 3306

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 16/57 (28%), Positives = 32/57 (56%)
 Frame = +3

Query: 375  KFIQVRLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLMRKRLKQRNVTLQAEPV 545
            +F+  +++++  L     + +  FI ++ FVN++  KL     RL++   TL +EPV
Sbjct: 1242 QFLHTKMRLKQELSVAPLEPSTSFIKFRIFVNLLTSKL----HRLRKAPGTLLSEPV 1294


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,988,814
Number of Sequences: 28952
Number of extensions: 214408
Number of successful extensions: 534
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 525
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 534
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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