Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0286.Seq
(627 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fat... 44 1e-04
At4g16800.1 68417.m02537 enoyl-CoA hydratase, putative similar t... 42 3e-04
At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP... 42 3e-04
At5g43280.1 68418.m05290 enoyl-CoA hydratase/isomerase family pr... 35 0.051
At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolas... 30 1.1
At4g36420.1 68417.m05174 ribosomal protein L12 family protein 30 1.1
At2g41750.1 68415.m05161 DTW domain-containing protein contains ... 30 1.1
At3g19320.1 68416.m02450 leucine-rich repeat family protein cont... 29 1.9
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 29 2.5
At4g33420.1 68417.m04749 peroxidase, putative identical to class... 29 3.3
At4g08780.1 68417.m01447 peroxidase, putative similar to peroxid... 29 3.3
At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolas... 28 4.4
At4g08113.1 68417.m01331 myosin heavy chain-related similar to M... 28 5.8
At1g60190.1 68414.m06780 armadillo/beta-catenin repeat family pr... 28 5.8
>At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fatty
acid multifunctional protein (AIM1) identical to
gi:4337025; contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 721
Score = 43.6 bits (98), Expect = 1e-04
Identities = 21/38 (55%), Positives = 26/38 (68%)
Frame = +1
Query: 511 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGG 624
LGGGLE A+AC R+A K GLPE+ LG++PG G
Sbjct: 111 LGGGLELAMACHARVAA--PKAQLGLPELTLGVIPGFG 146
>At4g16800.1 68417.m02537 enoyl-CoA hydratase, putative similar to
AU-binding protein/Enoyl-CoA hydratase [Homo sapiens]
GI:780241, [Mus musculus]GI:6840920; contains Pfam
profile PF00378 enoyl-CoA hydratase/isomerase family
protein
Length = 229
Score = 42.3 bits (95), Expect = 3e-04
Identities = 21/38 (55%), Positives = 26/38 (68%)
Frame = +1
Query: 511 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGG 624
LGGGLE ALAC RI +++ FGLPE L ++PG G
Sbjct: 78 LGGGLEMALACDLRICGENAV--FGLPETGLAIIPGAG 113
Score = 31.9 bits (69), Expect = 0.36
Identities = 22/77 (28%), Positives = 35/77 (45%)
Frame = +2
Query: 278 VMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENCKTKEEVVSLSKRGHEIF 457
+++ + N I ++ +I S PG F AGAD+ + EV + +F
Sbjct: 1 MLKSLQNAFESIHQDNSARVVMIRSLVPGVFCAGADLKE-RRTMSPSEVHTYVNSLRYMF 59
Query: 458 RRIEQSRKPYIAAIQGS 508
IE P IAAI+G+
Sbjct: 60 SFIEALSIPTIAAIEGA 76
>At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP2)
identical to fatty acid multifunctional protein (AtMFP2)
GB:AF123254 [gi:4337027] (Arabidopsis thaliana) (fatty
acid beta-oxidation); contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 725
Score = 41.9 bits (94), Expect = 3e-04
Identities = 20/38 (52%), Positives = 27/38 (71%)
Frame = +1
Query: 511 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGG 624
LGGGLE A+AC RI+ ++ GLPE+ LG++PG G
Sbjct: 114 LGGGLELAMACHARISAPAAQ--LGLPELQLGVIPGFG 149
Score = 32.3 bits (70), Expect = 0.27
Identities = 27/87 (31%), Positives = 43/87 (49%), Gaps = 4/87 (4%)
Frame = +2
Query: 257 VNSLNTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENCKTKEEVVSLS 436
VNSL+ V+ + + E + + ++A ++I+G G F G DIS + V
Sbjct: 28 VNSLSFDVLYNLKSNYEEALSRNDVKA-IVITGAKGRFSGGFDISGFGEMQKGN--VKEP 84
Query: 437 KRGHE----IFRRIEQSRKPYIAAIQG 505
K G+ I +E +RKP +AAI G
Sbjct: 85 KAGYISIDIITDLLEAARKPSVAAIDG 111
>At5g43280.1 68418.m05290 enoyl-CoA hydratase/isomerase family
protein similar to Delta 3,5-delta2,4-dienoyl-CoA
isomerase, mitochondrial (ECH1) from Rattus norvegicus
[SP|Q62651], from Homo sapiens [SP|Q13011]; contains
Pfam profile PF00378 enoyl-CoA hydratase/isomerase
family protein
Length = 278
Score = 34.7 bits (76), Expect = 0.051
Identities = 26/95 (27%), Positives = 45/95 (47%), Gaps = 11/95 (11%)
Frame = +2
Query: 257 VNSLNTQVMEEVSNIVNEIETNSGIEAAVIISGKPGCFIAGADISMIENCKTKEEVVSLS 436
+N+L+ E ++ ++ N + + +I+SG F +G D++ + + T+ +
Sbjct: 31 LNALSLDFFIEFPKALSSLDQNPDV-SVIILSGAGKHFCSGIDLNSLSSISTQSSSGNDR 89
Query: 437 KRGHEIFRR-----------IEQSRKPYIAAIQGS 508
R E RR IEQ RKP IAAI G+
Sbjct: 90 GRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIHGA 124
>At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolase /
CoA-thioester hydrolase (CHY1) identical to gi:8572760;
contains Pfam profile PF00388 enoyl-CoA
hydratase/isomerase family protein
Length = 378
Score = 30.3 bits (65), Expect = 1.1
Identities = 15/38 (39%), Positives = 23/38 (60%)
Frame = +1
Query: 511 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGG 624
+GGG ++ ++RIA ++ T F +PE LGL P G
Sbjct: 117 MGGGAGVSVHGRFRIATEN--TVFAMPETALGLFPDVG 152
>At4g36420.1 68417.m05174 ribosomal protein L12 family protein
Length = 179
Score = 30.3 bits (65), Expect = 1.1
Identities = 18/57 (31%), Positives = 34/57 (59%), Gaps = 2/57 (3%)
Frame = +3
Query: 75 NLKVMAN--SKILSALKILRSRKELFISGVHSRKYAVPASQVHTKCKLVNGVYVVTL 239
NL+++++ S++L + + S +LF + SR Y+ PA+Q K+VN + +TL
Sbjct: 3 NLRIISSHFSRVLKSTETRSSSVQLFT--IQSRSYSSPATQSENVSKIVNELSNLTL 57
>At2g41750.1 68415.m05161 DTW domain-containing protein contains
Pfam domain, PF03942: DTW domain
Length = 253
Score = 30.3 bits (65), Expect = 1.1
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2
Query: 587 SPNPVLESFTAMRYLHARAVSNPPPRRCLV 498
SP + F ++ L+ R +SNPPP R +V
Sbjct: 102 SPAVTISEFKSLNLLNHREISNPPPLRLIV 131
>At3g19320.1 68416.m02450 leucine-rich repeat family protein
contains leucine-rich repeats, Pfam:PF00560;
Length = 493
Score = 29.5 bits (63), Expect = 1.9
Identities = 14/38 (36%), Positives = 18/38 (47%)
Frame = -2
Query: 623 PPPGKSPNMTSGSPNPVLESFTAMRYLHARAVSNPPPR 510
PPP ++ SP+P E + Y H S PPPR
Sbjct: 70 PPPPPPQSLPPPSPSPEPEHYPPPPYHHYITPSPPPPR 107
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 29.1 bits (62), Expect = 2.5
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +1
Query: 511 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGG 624
+GGG + ++RIA ++ T F +PE LGL P G
Sbjct: 157 MGGGAGLSTNGRFRIATEN--TVFAMPETALGLFPDVG 192
>At4g33420.1 68417.m04749 peroxidase, putative identical to class
III peroxidase ATP32 [Arabidopsis thaliana]
gi|17530547|gb|AAL40837; identical to cDNA class III
peroxidase ATP32 GI:17530546
Length = 325
Score = 28.7 bits (61), Expect = 3.3
Identities = 17/59 (28%), Positives = 31/59 (52%), Gaps = 5/59 (8%)
Frame = +2
Query: 293 SNIVNEIETNSGIEAAVIISGKPGCFIAGADISMI-----ENCKTKEEVVSLSKRGHEI 454
+++ N ++ + + A +I CFI G D S++ +N K+ +LS RG+EI
Sbjct: 55 NSVNNALQADPTLAAGLIRMLFHDCFIEGCDASILLDSTKDNTAEKDSPANLSLRGYEI 113
>At4g08780.1 68417.m01447 peroxidase, putative similar to peroxidase
isozyme [Armoracia rusticana] gi|217932|dbj|BAA14143
Length = 346
Score = 28.7 bits (61), Expect = 3.3
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +2
Query: 230 SHFGLSKC*VNSLNTQVMEEVSN-IVNEIETNSGIEAAVIISGKPGCFIAGADISMI 397
SH LS + QV + V+N IVN + ++ I A+++ CF+ G D S++
Sbjct: 20 SHAQLSPSFYDKTCPQVFDIVTNTIVNALRSDPRIAASILRLHFHDCFVNGCDASIL 76
>At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 378
Score = 28.3 bits (60), Expect = 4.4
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +1
Query: 511 LGGGLETALACKYRIAVKDSKTGFGLPEVMLGLLPGGG 624
+G G ++ ++RIA ++ T F +PE LGL P G
Sbjct: 113 MGAGAGVSIHGRFRIATEN--TVFAMPETSLGLFPDVG 148
>At4g08113.1 68417.m01331 myosin heavy chain-related similar to
Myosin heavy chain, skeletal muscle, extraocular
(MyHC-eo) (SP:Q9UKX3) {Homo sapiens}
Length = 764
Score = 27.9 bits (59), Expect = 5.8
Identities = 14/58 (24%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
Frame = +2
Query: 308 EIETNS-GIEAAVIISGKPGCFIAGADISMIENCKTKEEVVSLSKRGHEIFRRIEQSR 478
+++TN+ + IS +PG + GA + +T+ E+++L +RG + ++R
Sbjct: 173 QVKTNARSDQGTYYISARPGSLVLGARTKEVTLSETELELLTLLQRGQTNWSTFSRNR 230
>At1g60190.1 68414.m06780 armadillo/beta-catenin repeat family
protein / U-box domain-containing protein contains Pfam
domain, PF00514: Armadillo/beta-catenin-like repeats and
Pfam, PF04564: U-box domain
Length = 686
Score = 27.9 bits (59), Expect = 5.8
Identities = 14/47 (29%), Positives = 25/47 (53%)
Frame = +1
Query: 451 NIQKNRTITETVYCRNTRQRLGGGLETALACKYRIAVKDSKTGFGLP 591
++ KN +I ++Y ++ LGGG + + K ++ KTG G P
Sbjct: 629 SLAKNPSIMGSLYTASSNGELGGGKKASALIKMIHEFQERKTGPGEP 675
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,721,819
Number of Sequences: 28952
Number of extensions: 262794
Number of successful extensions: 768
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 763
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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