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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0253.Seq
         (465 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g14520.1 68415.m01625 CBS domain-containing protein contains ...    28   3.6  
At2g01340.1 68415.m00051 expressed protein                             27   6.2  
At2g33560.1 68415.m04113 spindle checkpoint protein-related simi...    27   8.3  

>At2g14520.1 68415.m01625 CBS domain-containing protein contains
           Pfam profiles PF00571: CBS domain, PF01595: Domain of
           unknown function
          Length = 423

 Score = 27.9 bits (59), Expect = 3.6
 Identities = 11/43 (25%), Positives = 23/43 (53%)
 Frame = +1

Query: 310 KGPVEPQNRRRRRGSTWEAWCGKYRSHGPKARRFHHDRQGSVL 438
           + P E + +RRR    W+++  +  S G +++R+  D    +L
Sbjct: 336 RSPQETKLKRRRSLQKWKSFPNRANSLGSRSKRWSKDNDADIL 378


>At2g01340.1 68415.m00051 expressed protein 
          Length = 215

 Score = 27.1 bits (57), Expect = 6.2
 Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = +2

Query: 227 PTKESPPPS-RDGVHDASSEERLGHIQLSKVPSS 325
           P KE PP   R G+H  S++ERL  + L++  SS
Sbjct: 74  PVKECPPRRVRSGIH-VSAKERLESLMLARRSSS 106


>At2g33560.1 68415.m04113 spindle checkpoint protein-related similar
           to spindle checkpoint protein BubR1 (GI:22128593)
           [Xenopus laevis]; similar to Mitotic checkpoint
           serine/threonine-protein kinase BUB1 beta (EC 2.7.1.-)
           (MAD3/BUB1-related protein kinase) (Mitotic checkpoint
           kinase MAD3L) (Swiss-Prot:Q9Z1S0) [Mus musculus]
          Length = 395

 Score = 26.6 bits (56), Expect = 8.3
 Identities = 20/88 (22%), Positives = 38/88 (43%), Gaps = 2/88 (2%)
 Frame = -2

Query: 326 GSTGPLRVVYVPIVLHYWHHERHLAKVAVTLLLERY*RNCLR*DE-KKYVSVNKL-NDK* 153
           G    L V+Y   V  +WH ER+   +    +   Y  +C   +   K++ VN++     
Sbjct: 100 GECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEHCADAEVIYKFLEVNEIGKTHA 159

Query: 152 *Y*FYFSLNAYISGKFQVSVEIYRSNLS 69
            Y   ++L+     K + + EI+   +S
Sbjct: 160 VYYIAYALHIEFKNKVKTANEIFNLGIS 187


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,659,636
Number of Sequences: 28952
Number of extensions: 183740
Number of successful extensions: 562
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 553
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 562
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 782033640
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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