BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0253.Seq (465 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g14520.1 68415.m01625 CBS domain-containing protein contains ... 28 3.6 At2g01340.1 68415.m00051 expressed protein 27 6.2 At2g33560.1 68415.m04113 spindle checkpoint protein-related simi... 27 8.3 >At2g14520.1 68415.m01625 CBS domain-containing protein contains Pfam profiles PF00571: CBS domain, PF01595: Domain of unknown function Length = 423 Score = 27.9 bits (59), Expect = 3.6 Identities = 11/43 (25%), Positives = 23/43 (53%) Frame = +1 Query: 310 KGPVEPQNRRRRRGSTWEAWCGKYRSHGPKARRFHHDRQGSVL 438 + P E + +RRR W+++ + S G +++R+ D +L Sbjct: 336 RSPQETKLKRRRSLQKWKSFPNRANSLGSRSKRWSKDNDADIL 378 >At2g01340.1 68415.m00051 expressed protein Length = 215 Score = 27.1 bits (57), Expect = 6.2 Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%) Frame = +2 Query: 227 PTKESPPPS-RDGVHDASSEERLGHIQLSKVPSS 325 P KE PP R G+H S++ERL + L++ SS Sbjct: 74 PVKECPPRRVRSGIH-VSAKERLESLMLARRSSS 106 >At2g33560.1 68415.m04113 spindle checkpoint protein-related similar to spindle checkpoint protein BubR1 (GI:22128593) [Xenopus laevis]; similar to Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (EC 2.7.1.-) (MAD3/BUB1-related protein kinase) (Mitotic checkpoint kinase MAD3L) (Swiss-Prot:Q9Z1S0) [Mus musculus] Length = 395 Score = 26.6 bits (56), Expect = 8.3 Identities = 20/88 (22%), Positives = 38/88 (43%), Gaps = 2/88 (2%) Frame = -2 Query: 326 GSTGPLRVVYVPIVLHYWHHERHLAKVAVTLLLERY*RNCLR*DE-KKYVSVNKL-NDK* 153 G L V+Y V +WH ER+ + + Y +C + K++ VN++ Sbjct: 100 GECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEHCADAEVIYKFLEVNEIGKTHA 159 Query: 152 *Y*FYFSLNAYISGKFQVSVEIYRSNLS 69 Y ++L+ K + + EI+ +S Sbjct: 160 VYYIAYALHIEFKNKVKTANEIFNLGIS 187 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,659,636 Number of Sequences: 28952 Number of extensions: 183740 Number of successful extensions: 562 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 553 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 562 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 782033640 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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