Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0247.Seq
(705 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g47480.1 68418.m05863 expressed protein 33 0.24
At5g45520.1 68418.m05591 hypothetical protein 32 0.32
At5g24880.1 68418.m02946 expressed protein ; expression supporte... 31 0.56
At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein... 30 1.3
At4g26130.1 68417.m03761 expressed protein 29 2.3
At3g28770.1 68416.m03591 expressed protein 29 2.3
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro... 29 2.3
At3g51070.1 68416.m05592 dehydration-responsive protein-related ... 29 4.0
At4g31880.1 68417.m04531 expressed protein 28 5.2
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 28 5.2
At5g01380.1 68418.m00051 expressed protein 28 6.9
At5g64500.1 68418.m08103 membrane protein-related contains weak ... 27 9.2
At5g62550.1 68418.m07850 expressed protein 27 9.2
At5g11710.1 68418.m01368 epsin N-terminal homology (ENTH) domain... 27 9.2
At4g39840.1 68417.m05645 expressed protein 27 9.2
At1g75310.1 68414.m08748 DNAJ heat shock N-terminal domain-conta... 27 9.2
At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /... 27 9.2
>At5g47480.1 68418.m05863 expressed protein
Length = 1350
Score = 32.7 bits (71), Expect = 0.24
Identities = 23/80 (28%), Positives = 35/80 (43%)
Frame = +1
Query: 22 PTKNQTKIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSFGIQEEDK 201
P ++T I DQT+SS KS ++ S+ G GI KNT F + ++
Sbjct: 1032 PDFSRTPIQDQTDSSKDKAPDGVTQVKSTRKVPSSRFSRFGIGILKNTVGKVFPSRSSNE 1091
Query: 202 VKSPEEEALSAFVYPETSRR 261
K E + F Y + +R
Sbjct: 1092 AKLGNE---NQFYYDDNLKR 1108
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 32.3 bits (70), Expect = 0.32
Identities = 21/91 (23%), Positives = 38/91 (41%)
Frame = +1
Query: 1 EKEPTKIPTKNQTKIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSF 180
E+E ++ QT +++ N ++ I+ + + KK + S S
Sbjct: 584 EREDDQVQNYGQTSKEEKGNVEETGKQEDGDQGDGINEEANLEDGKKHDEGKEERSLKSD 643
Query: 181 GIQEEDKVKSPEEEALSAFVYPETSRRSKSH 273
+ EE+K SP EEA F ++ KS+
Sbjct: 644 EVVEEEKKTSPSEEATEKFQNKPGDQKGKSN 674
>At5g24880.1 68418.m02946 expressed protein ; expression supported
by MPSS
Length = 443
Score = 31.5 bits (68), Expect = 0.56
Identities = 29/131 (22%), Positives = 52/131 (39%), Gaps = 2/131 (1%)
Frame = +1
Query: 40 KIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKK-GKGIYKNTSTDSFGIQEEDKVKSPE 216
KID+ + E + + +TQE+ + K+ GK + + ++E+D+ + E
Sbjct: 308 KIDENETPEKVDTESKEVESVEETTQEKEEEVKEEGKERVEEEEKEKEKVKEDDQKEKVE 367
Query: 217 EEALSAFVYPETSRRSKSH*TFYSTRKARTE-PTESPKLRK**TVEQLRVLPAKRKVGRM 393
EE E + K + +K + ESP +++ P K KV +
Sbjct: 368 EEEKEKVKGDEEKEKVKEEESAEGKKKEVVKGKKESPSAYNDVIASKMQENPRKNKVLAL 427
Query: 394 RGEIQTCTHQE 426
G QT E
Sbjct: 428 AGAFQTVIDYE 438
>At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 551
Score = 30.3 bits (65), Expect = 1.3
Identities = 22/88 (25%), Positives = 39/88 (44%)
Frame = +1
Query: 73 NNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSFGIQEEDKVKSPEEEALSAFVYPET 252
N+ Q + + ST K+ KK K +N+ DS + K K +++ P+T
Sbjct: 373 NSSQVSKRDRETSTTSH-KSRKKNK---ENSEHDSTPHESNGKTKKKKKKKTHKEEQPQT 428
Query: 253 SRRSKSH*TFYSTRKARTEPTESPKLRK 336
S R + H + T + E + K+R+
Sbjct: 429 SPRKRKHRGGWITEEPEEESFQRGKMRR 456
>At4g26130.1 68417.m03761 expressed protein
Length = 286
Score = 29.5 bits (63), Expect = 2.3
Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +1
Query: 82 QTTMPTKSISTQEQTKTSKKG-KGIYKNTSTDSFGIQEEDKVKSPEE 219
QT TKS S++ TK KK K + K+ S G +EE+ V++ E+
Sbjct: 185 QTHTRTKSESSKPATKKKKKATKKMMKSASERHIGREEEETVEAVEK 231
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 29.5 bits (63), Expect = 2.3
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = +1
Query: 1 EKEPTKIPTKNQTKIDDQTNSSTKNNEQTTMPTKSISTQEQTK--TSKKGKGIYKNTSTD 174
E + + + NQ D +N ++ NN+ T IST T+ TSK+ N
Sbjct: 1838 ETKESNVSMNNQNMQDVGSNENSMNNQTTGTGDDIISTTTDTESNTSKEVTSFISNLEEK 1897
Query: 175 SFGIQE 192
S G QE
Sbjct: 1898 SPGTQE 1903
>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
protein GI:1279562 from [Medicago sativa]
Length = 557
Score = 29.5 bits (63), Expect = 2.3
Identities = 18/75 (24%), Positives = 38/75 (50%)
Frame = +1
Query: 1 EKEPTKIPTKNQTKIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSF 180
EK TK K ++SS +++++ + K + TK SKK + S++S
Sbjct: 198 EKPATKKAAPAAAKAASSSDSSDEDSDEESEDEKPAQKKADTKASKKSS---SDESSESE 254
Query: 181 GIQEEDKVKSPEEEA 225
+ ED+ ++P++++
Sbjct: 255 EDESEDEEETPKKKS 269
>At3g51070.1 68416.m05592 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 895
Score = 28.7 bits (61), Expect = 4.0
Identities = 17/67 (25%), Positives = 32/67 (47%)
Frame = +1
Query: 1 EKEPTKIPTKNQTKIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSF 180
E P K+P D+Q S+ + +E T+ T++ TQ Q K + K+ ++
Sbjct: 88 EDNPGKLPDDAVKSEDEQRKSAKEKSETTSSKTQTQETQ-QNNDDKISEEKEKDNGKENQ 146
Query: 181 GIQEEDK 201
+QE ++
Sbjct: 147 TVQESEE 153
>At4g31880.1 68417.m04531 expressed protein
Length = 873
Score = 28.3 bits (60), Expect = 5.2
Identities = 33/133 (24%), Positives = 58/133 (43%), Gaps = 8/133 (6%)
Frame = +1
Query: 16 KIPTKNQTKIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSFGIQEE 195
K T Q+K+D+ +S+ K + + K+ + +KTS+ K K+ + +EE
Sbjct: 689 KAKTGKQSKMDN--SSAKKGSGAGSSKAKATPASKSSKTSQDDKTASKSKDSKEASREEE 746
Query: 196 -DKVKSPEEEALSAFVYPETSRRSKSH*TFYS-------TRKARTEPTESPKLRK**TVE 351
+ EEE V S RSK + S + K + EP+++ K
Sbjct: 747 ASSEEESEEEEPPKTVGKSGSSRSKKDISSVSKSGKSKASSKKKEEPSKATTSSK-SKSG 805
Query: 352 QLRVLPAKRKVGR 390
++ +PAK K G+
Sbjct: 806 PVKSVPAKSKTGK 818
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 28.3 bits (60), Expect = 5.2
Identities = 16/67 (23%), Positives = 33/67 (49%)
Frame = +1
Query: 49 DQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSFGIQEEDKVKSPEEEAL 228
++T+ + N ++ P IS + S KGK + ++ D+ G ++D+ +EE +
Sbjct: 116 EETDDNESNGDKDDEPV--ISFTGKKNASNKGKKGFAVSAFDALGGDKDDEEVDGDEEQV 173
Query: 229 SAFVYPE 249
S + E
Sbjct: 174 SPITFSE 180
>At5g01380.1 68418.m00051 expressed protein
Length = 323
Score = 27.9 bits (59), Expect = 6.9
Identities = 16/61 (26%), Positives = 29/61 (47%)
Frame = +1
Query: 1 EKEPTKIPTKNQTKIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSF 180
+ E ++ NQ ++ + +T + T S ST + K +KKGKG+ T ++
Sbjct: 165 DDEEEEVDEPNQDINEELLSLVETQKRETEVITTSTSTNPR-KRAKKGKGVASGTKAETA 223
Query: 181 G 183
G
Sbjct: 224 G 224
>At5g64500.1 68418.m08103 membrane protein-related contains weak
similarity to spinster type IV (GI:12003976) [Drosophila
melanogaster]
Length = 484
Score = 27.5 bits (58), Expect = 9.2
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
Frame = -3
Query: 157 YIYLCLSSTFSFVPVSK*TLLASLF---VRCFSSMNLFDRLFSFGSSLVF 17
Y+ +S+ F + VS T + ++F CF SM F LF+ G LVF
Sbjct: 333 YMDATISNAFKVLSVS--TFIGAIFCFAAFCFKSMYAFLALFAVGELLVF 380
>At5g62550.1 68418.m07850 expressed protein
Length = 487
Score = 27.5 bits (58), Expect = 9.2
Identities = 20/79 (25%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +1
Query: 37 TKIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDSFGIQEEDKVKSPE 216
TK +D S+ +TT +++ T Q +T K + I N DS +QE ++ P
Sbjct: 317 TKEEDALQDSSVT--ETTKEEQALETVTQGRTRKSLEVINVNQENDSEVVQESEEGLRPS 374
Query: 217 EEALS--AFVYPETSRRSK 267
+ + V P +R++
Sbjct: 375 ADGVQIVTVVKPSDKKRAR 393
>At5g11710.1 68418.m01368 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related contains Pfam PF01417: ENTH domain. ENTH
(Epsin N-terminal homology) domain; similar to
Af10-protein (GI:1724114) [Avena fatua]; similar to
clathrin assembly protein AP180 (GI:6492344) [Xenopus
laevis]; Af10-protein, Avena fatua, EMBL:U80041
Length = 560
Score = 27.5 bits (58), Expect = 9.2
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 3/64 (4%)
Frame = +1
Query: 25 TKNQTKIDDQTNSSTKNNEQTTMPTK-SISTQEQTKTSKKGKGIYKNTSTD--SFGIQEE 195
+ N D+ + KN+ ++ ++ + T+EQ+ TSKK Y +T D S G +
Sbjct: 188 SSNFDSYKDRDSREDKNDYESFQKSRRGVKTEEQSYTSKKSFSRYGSTDHDNLSSGKKSP 247
Query: 196 DKVK 207
D K
Sbjct: 248 DSAK 251
>At4g39840.1 68417.m05645 expressed protein
Length = 451
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = +1
Query: 25 TKNQTKIDDQTNSSTKNNEQTTMPTKSISTQEQTKTSKKGKGIYKNTSTDS 177
TKNQTK+ T T + +T + S +T+ ++ K G ST S
Sbjct: 107 TKNQTKLAKTTTMGTSHKLNST-KSSSNTTKTSSELKKLNSGTKSTNSTSS 156
>At1g75310.1 68414.m08748 DNAJ heat shock N-terminal
domain-containing protein low similarity to SP|Q27974
Auxilin {Bos taurus}; contains Pfam profile PF00226:
DnaJ domain
Length = 1448
Score = 27.5 bits (58), Expect = 9.2
Identities = 22/85 (25%), Positives = 40/85 (47%), Gaps = 12/85 (14%)
Frame = +1
Query: 7 EPTKIPTKNQTKIDDQTNSSTKNNEQ----TTMPTKSISTQEQTKTSKKGK-------GI 153
+ +KI K TK++ T S NN Q P++ + E + +K+ + G+
Sbjct: 356 DDSKIENKGNTKVEGITEESRDNNSQILGEMVKPSEQSFSNEGDQHAKRARKLWGVPEGL 415
Query: 154 YKNTSTDS-FGIQEEDKVKSPEEEA 225
K+TS ++E+D + EE+A
Sbjct: 416 LKSTSDHKPEELEEQDIITLEEEQA 440
>At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
SEC14-like protein 2 (Alpha-tocopherol associated
protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos
taurus}; similar to GI:807956 from [Saccharomyces
cerevisiae]; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 573
Score = 27.5 bits (58), Expect = 9.2
Identities = 17/57 (29%), Positives = 28/57 (49%)
Frame = +1
Query: 97 TKSISTQEQTKTSKKGKGIYKNTSTDSFGIQEEDKVKSPEEEALSAFVYPETSRRSK 267
T+ T+E+TK +K + + T+T+ +E+ V + EEE S ET K
Sbjct: 125 TEEKKTEEETKEEEKTEEKKEETTTEVKVEEEKPAVPAAEEEKSSEAAPVETKSEEK 181
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,231,125
Number of Sequences: 28952
Number of extensions: 249455
Number of successful extensions: 791
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 790
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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