Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0240.Seq
(704 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 149 3e-37
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 146 2e-36
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 81 2e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 55 1e-08
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.21
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.5
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 27 3.5
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.6
SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe... 26 4.6
SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 26 4.6
SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.6
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 4.6
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.0
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 26 6.0
SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 6.0
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 8.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 149 bits (362), Expect = 3e-37
Identities = 67/82 (81%), Positives = 74/82 (90%)
Frame = +3
Query: 258 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 437
Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV EIT
Sbjct: 233 QVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEIT 292
Query: 438 NACFEPANQMVKCDPRHGKYMA 503
N CFEP NQMVKCDPR G+YMA
Sbjct: 293 NQCFEPYNQMVKCDPRAGRYMA 314
Score = 141 bits (341), Expect = 1e-34
Identities = 61/82 (74%), Positives = 75/82 (91%)
Frame = +1
Query: 1 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 180
SGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDN
Sbjct: 147 SGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDN 206
Query: 181 EAIYDICRRNLDIERPTYTNLN 246
E+ YDICRRNLDIERP+Y NLN
Sbjct: 207 ESCYDICRRNLDIERPSYENLN 228
Score = 108 bits (259), Expect = 9e-25
Identities = 46/65 (70%), Positives = 55/65 (84%)
Frame = +2
Query: 509 MLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 688
+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++AKV RAVC
Sbjct: 317 LLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVC 376
Query: 689 MLSNT 703
MLSNT
Sbjct: 377 MLSNT 381
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 146 bits (355), Expect = 2e-36
Identities = 66/82 (80%), Positives = 73/82 (89%)
Frame = +3
Query: 258 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 437
Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV EIT
Sbjct: 237 QVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEIT 296
Query: 438 NACFEPANQMVKCDPRHGKYMA 503
N CFEP NQMVKCDPR G+YMA
Sbjct: 297 NQCFEPYNQMVKCDPRTGRYMA 318
Score = 145 bits (352), Expect = 5e-36
Identities = 63/82 (76%), Positives = 75/82 (91%)
Frame = +1
Query: 1 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 180
SG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDN
Sbjct: 151 SGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN 210
Query: 181 EAIYDICRRNLDIERPTYTNLN 246
EA YDICRRNLDIERPTY NLN
Sbjct: 211 EACYDICRRNLDIERPTYENLN 232
Score = 112 bits (270), Expect = 4e-26
Identities = 47/65 (72%), Positives = 57/65 (87%)
Frame = +2
Query: 509 MLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 688
+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV RAVC
Sbjct: 321 LLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVC 380
Query: 689 MLSNT 703
MLSNT
Sbjct: 381 MLSNT 385
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 80.6 bits (190), Expect = 2e-16
Identities = 33/80 (41%), Positives = 52/80 (65%)
Frame = +3
Query: 261 IVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITN 440
+++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ + + + +SV E+T
Sbjct: 232 VMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQ 291
Query: 441 ACFEPANQMVKCDPRHGKYM 500
F+ N MV DPRHG+Y+
Sbjct: 292 QMFDANNMMVAADPRHGRYL 311
Score = 72.5 bits (170), Expect = 5e-14
Identities = 33/82 (40%), Positives = 51/82 (62%)
Frame = +1
Query: 1 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 180
SG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DN
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDN 204
Query: 181 EAIYDICRRNLDIERPTYTNLN 246
EA+ I L I+ P+Y +LN
Sbjct: 205 EALSSIFANTLKIKSPSYDDLN 226
Score = 35.1 bits (77), Expect = 0.010
Identities = 15/43 (34%), Positives = 24/43 (55%)
Frame = +2
Query: 512 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 640
L+RG V K+V+ I +++TK + FV+W P + PP
Sbjct: 316 LFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPP 358
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 54.8 bits (126), Expect = 1e-08
Identities = 30/83 (36%), Positives = 45/83 (54%), Gaps = 1/83 (1%)
Frame = +1
Query: 1 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVD 177
SG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++D
Sbjct: 147 SGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLD 206
Query: 178 NEAIYDICRRNLDIERPTYTNLN 246
N A+ I L + PT+ N
Sbjct: 207 NAALAHIAADRLHTQNPTFHQQN 229
Score = 54.0 bits (124), Expect = 2e-08
Identities = 28/89 (31%), Positives = 43/89 (48%), Gaps = 3/89 (3%)
Frame = +3
Query: 225 PNLHQPESSHGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---A 395
P HQ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P +
Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVE 282
Query: 396 EKAYHEQLSVAEITNACFEPANQMVKCDP 482
E + +V ++ P NQMV +P
Sbjct: 283 EAKAIRKTTVLDVMRRLLLPKNQMVSVNP 311
Score = 33.5 bits (73), Expect = 0.030
Identities = 12/48 (25%), Positives = 27/48 (56%)
Frame = +2
Query: 503 LLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTV 646
+L + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P +
Sbjct: 320 ILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYI 367
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 30.7 bits (66), Expect = 0.21
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = +3
Query: 519 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 653
VV P + RP++P P LS V PV+ V + PP P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.9 bits (59), Expect = 1.5
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = +1
Query: 319 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 465
P+++ P S + T +SS+P P N+ PSP S + AS+ P R
Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279
Score = 25.4 bits (53), Expect = 8.0
Identities = 23/63 (36%), Positives = 29/63 (46%), Gaps = 3/63 (4%)
Frame = +3
Query: 525 TSYPRM*TRPSLP-SKPSVLSNSS--TGVQPVSRSVSTTSHPPWCPEATWPRFNVPSACC 695
TS P PS P SKP S ++ V PV + +S+ + P P A P VPS
Sbjct: 983 TSEPPKDHPPSAPLSKPVSTSPAAPLARVPPVPK-LSSKAPPVPLPSADAPPIPVPSTAP 1041
Query: 696 PTP 704
P P
Sbjct: 1042 PVP 1044
>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 793
Score = 26.6 bits (56), Expect = 3.5
Identities = 18/64 (28%), Positives = 24/64 (37%)
Frame = +2
Query: 488 WQVHGLLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLA 667
W+ Y + KDVN + TI +W PTG ++ Y V D
Sbjct: 141 WRHSSFAQYYLYNTETKDVNM-LGQDNEHWTISLAEWSPTGHQLSFVYNNDLYVRKND-G 198
Query: 668 KVQR 679
VQR
Sbjct: 199 NVQR 202
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 26.2 bits (55), Expect = 4.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 382 QSSLPRRPTMNSFPSPRSQTHASSPPTR 465
+ SLPRRP+ +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765
>SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 526
Score = 26.2 bits (55), Expect = 4.6
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 551 PRSHPWVRRHHGTASASHVLAM 486
P + WV RH G+ SA H L +
Sbjct: 468 PWVYEWVSRHRGSISAEHGLGL 489
>SPAC3C7.09 |set8||lysine methyltransferase Set8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 429
Score = 26.2 bits (55), Expect = 4.6
Identities = 7/17 (41%), Positives = 13/17 (76%)
Frame = -1
Query: 686 RRHVEPWPSRLRAPRWV 636
+R+ + WPS++ P+WV
Sbjct: 153 QRYSDSWPSKITLPKWV 169
>SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1339
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/47 (29%), Positives = 27/47 (57%)
Frame = +2
Query: 494 VHGLLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQ 634
V G + L R ++ + N AIA+I +I+F ++ P GF ++++
Sbjct: 294 VRGAVRLMRFLMLQEVKNDAIASITLSSSIEFTEF-PLGFNENVDFE 339
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = +3
Query: 555 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 641
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 751
Score = 25.8 bits (54), Expect = 6.0
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +1
Query: 316 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 456
SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 4/40 (10%)
Frame = +2
Query: 170 WSTMKPSMTSAAVISTLSAQPTPT*IVSWTD----CLLDH 277
+S+ KP+ + ++ S+Q TPT + W + CL+ H
Sbjct: 1904 FSSSKPAEVAKLLLEVYSSQDTPTKLNEWANYFMLCLIRH 1943
>SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr
1|||Manual
Length = 474
Score = 25.8 bits (54), Expect = 6.0
Identities = 16/51 (31%), Positives = 31/51 (60%)
Frame = +1
Query: 343 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 495
LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++
Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 8.0
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = +3
Query: 552 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 647
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,084,444
Number of Sequences: 5004
Number of extensions: 66374
Number of successful extensions: 245
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 244
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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