Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0226.Seq
         (544 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R...    79   2e-15
At4g28470.1 68417.m04073 26S proteasome regulatory subunit, puta...    50   8e-07
At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase fa...    29   1.5  
At1g64680.1 68414.m07332 expressed protein                             28   3.5  
At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-...    27   8.1  
At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-...    27   8.1  

>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
           (RPN1) contains an APC-complex (cyclosome) and
           proteasome component repeat ( PS50248)
          Length = 891

 Score = 79.0 bits (186), Expect = 2e-15
 Identities = 38/88 (43%), Positives = 56/88 (63%)
 Frame = +3

Query: 249 PALSVIEVLNIYSHDSDNDVAYNSIFAMGLVGAGTNNXXXXXXXXXXXXYHGKSPVHLFM 428
           P ++V++ L+  SHD+D++VA ++I ++GL+GAGTNN            Y+ K    LF 
Sbjct: 684 PKVTVMDTLSRLSHDTDSEVAMSAIISLGLIGAGTNNARIAGMLRNLSSYYYKDMSLLFC 743

Query: 429 VLLAQGLCHAGKGTVTLCPAHANRRLLT 512
           V +AQGL H GKG +TL P H+ R LL+
Sbjct: 744 VRIAQGLVHMGKGLLTLSPFHSERFLLS 771



 Score = 52.0 bits (119), Expect = 2e-07
 Identities = 24/53 (45%), Positives = 36/53 (67%)
 Frame = +1

Query: 94  QAVATLGVAVIALAAETGAEMCTRIFGQLGRYGEPVVRRAVPLAIALCSVSNP 252
           Q  A LG+A++A++ E G +M  R   ++ +YGE  +RRAVPLA+ L  +SNP
Sbjct: 632 QGPAVLGLAMVAMSEELGVDMEIRSLERMLQYGEQNIRRAVPLALGLLCISNP 684


>At4g28470.1 68417.m04073 26S proteasome regulatory subunit,
           putative contains Pfam domain PF01851:
           Proteasome/cyclosome repeat
          Length = 1103

 Score = 50.4 bits (115), Expect = 8e-07
 Identities = 23/53 (43%), Positives = 36/53 (67%)
 Frame = +1

Query: 94  QAVATLGVAVIALAAETGAEMCTRIFGQLGRYGEPVVRRAVPLAIALCSVSNP 252
           Q  A +G+A++A++ E G +M  R   ++ +YGE  +RRAVPLA+ L  +SNP
Sbjct: 704 QGPAVIGLAMVAMSEELGLDMEIRSLERVLQYGEQNIRRAVPLALGLLCISNP 756



 Score = 48.0 bits (109), Expect = 4e-06
 Identities = 23/61 (37%), Positives = 36/61 (59%)
 Frame = +3

Query: 249 PALSVIEVLNIYSHDSDNDVAYNSIFAMGLVGAGTNNXXXXXXXXXXXXYHGKSPVHLFM 428
           P ++V++ L+  SHD+D++VA  +I ++GL+GAGTNN            Y+ K    LF 
Sbjct: 756 PKVTVMDTLSRLSHDTDSEVAMAAIISLGLIGAGTNNARIAGMLRNLSSYYYKDASLLFC 815

Query: 429 V 431
           V
Sbjct: 816 V 816


>At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein similar to flavanone 3-hydroxylase
           [Persea americana][GI:727410]; contains PF03171
           2OG-Fe(II) oxygenase superfamily domain
          Length = 341

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 18/70 (25%), Positives = 28/70 (40%), Gaps = 1/70 (1%)
 Frame = -3

Query: 299 IRIVGVYIEHFDDGECGLDTEHRAMASGTARRTTGSPYLPSCPKMRVHISAPV-SAANAM 123
           +R VG  IE       GL+ ++     G   +     Y P CP+  +    P  +  NA+
Sbjct: 159 VREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNAL 218

Query: 122 TATPRVATAC 93
           T   +  T C
Sbjct: 219 TILLQDTTVC 228


>At1g64680.1 68414.m07332 expressed protein
          Length = 250

 Score = 28.3 bits (60), Expect = 3.5
 Identities = 14/35 (40%), Positives = 17/35 (48%)
 Frame = +1

Query: 157 CTRIFGQLGRYGEPVVRRAVPLAIALCSVSNPHSP 261
           C  I+GQ     E  V    P    +CS+SNP SP
Sbjct: 209 CEMIYGQAPPAFEEDVATKQPCLADICSMSNPSSP 243


>At4g31490.1 68417.m04473 coatomer beta subunit, putative /
           beta-coat protein, putative / beta-COP, putative similar
           to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
           from {Rattus norvegicus} SP|P23514, {Mus musculus}
           SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 948

 Score = 27.1 bits (57), Expect = 8.1
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = -1

Query: 433 STMNRCTGDLPWYSASARS 377
           ST+ +C G+LP+YS S  S
Sbjct: 472 STITQCLGELPFYSVSEES 490


>At4g31480.1 68417.m04472 coatomer beta subunit, putative /
           beta-coat protein, putative / beta-COP, putative similar
           to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
           from {Rattus norvegicus} SP|P23514, {Mus musculus}
           SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 971

 Score = 27.1 bits (57), Expect = 8.1
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = -1

Query: 433 STMNRCTGDLPWYSASARS 377
           ST+ +C G+LP+YS S  S
Sbjct: 495 STITQCLGELPFYSVSEES 513


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,684,715
Number of Sequences: 28952
Number of extensions: 174664
Number of successful extensions: 471
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 461
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 471
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1013649368
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)