Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0223.Seq
(782 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd... 89 4e-18
At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd... 88 5e-18
At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd... 79 3e-15
At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd... 79 4e-15
At1g21200.1 68414.m02650 expressed protein 31 0.86
At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 fac... 29 4.6
At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 fac... 29 4.6
At5g64560.2 68418.m08114 magnesium transporter CorA-like family ... 28 6.1
At5g64560.1 68418.m08113 magnesium transporter CorA-like family ... 28 6.1
At2g29250.1 68415.m03554 lectin protein kinase, putative similar... 28 6.1
At3g45950.1 68416.m04972 splicing factor-related similar to step... 28 8.0
>At4g00620.1 68417.m00086 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
(C1-THF synthase) [Includes: Methylenetetrahydrofolate
dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
contains Pfam profiles PF02882: Tetrahydrofolate
dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
catalytic domain
Length = 360
Score = 88.6 bits (210), Expect = 4e-18
Identities = 42/85 (49%), Positives = 60/85 (70%), Gaps = 2/85 (2%)
Frame = +1
Query: 511 VQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLCVD--LPTIVPATALAVVEMLKRFN 684
VQLP+P ++E+ + +A++ EKD+DGFH +NIGRL + P VP T +E+L R+N
Sbjct: 163 VQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYN 222
Query: 685 IDTFGRNAVVIGRSKNVGMPIAMML 759
I+ G+ AVVIGRS VGMP A++L
Sbjct: 223 IEIKGKRAVVIGRSNIVGMPAALLL 247
Score = 56.0 bits (129), Expect = 3e-08
Identities = 28/87 (32%), Positives = 49/87 (56%)
Frame = +2
Query: 248 QSIAMEIKKELKQKIAQWVSLGNRAPTIRCIIVGDDPASHTYVRNKVEAAKFVGIDALTI 427
+++A +I+ E+ ++++ P + I+VGD S TYVRNK +A VGI + +
Sbjct: 75 KAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSATYVRNKKKACDSVGIKSFEV 134
Query: 428 NRDSDITEEQLLSEIQNLNEDNNVDGI 508
D +EE++L + N+D +V GI
Sbjct: 135 RLAEDSSEEEVLKSVSGFNDDPSVHGI 161
>At4g00600.1 68417.m00084 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
precursor (C1-THF synthase) [Includes:
Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
Formyltetrahydrofolate synthetase (EC 6.3.4.3)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
NAD(P)-binding domain, PF00763: Tetrahydrofolate
dehydrogenase/cyclohydrolase, catalytic domain
Length = 310
Score = 88.2 bits (209), Expect = 5e-18
Identities = 42/85 (49%), Positives = 60/85 (70%), Gaps = 2/85 (2%)
Frame = +1
Query: 511 VQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLCVD--LPTIVPATALAVVEMLKRFN 684
VQLP+P ++E+ + +A++ EKD+DGFH +NIGRL + P VP T +E+L R+N
Sbjct: 113 VQLPLPSHMDEQNILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYN 172
Query: 685 IDTFGRNAVVIGRSKNVGMPIAMML 759
I+ G+ AVVIGRS VGMP A++L
Sbjct: 173 IEFKGKRAVVIGRSNIVGMPAALLL 197
>At3g12290.1 68416.m01534 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
(C1-THF synthase) [Includes: Methylenetetrahydrofolate
dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
contains Pfam profiles PF02882: Tetrahydrofolate
dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
catalytic domain
Length = 299
Score = 79.0 bits (186), Expect = 3e-15
Identities = 37/85 (43%), Positives = 56/85 (65%), Gaps = 2/85 (2%)
Frame = +1
Query: 511 VQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLCVD--LPTIVPATALAVVEMLKRFN 684
VQLP+P INE + AI+ +KD+DGFH +NIG+L + P +P T +E+L R
Sbjct: 103 VQLPLPKHINEEHILGAISIDKDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSG 162
Query: 685 IDTFGRNAVVIGRSKNVGMPIAMML 759
+ G+ AVV+GRS VG+P++++L
Sbjct: 163 VKIKGQRAVVVGRSNIVGLPVSLLL 187
Score = 51.2 bits (117), Expect = 8e-07
Identities = 27/101 (26%), Positives = 49/101 (48%)
Frame = +2
Query: 206 LCKHSKHHGAYPRWQSIAMEIKKELKQKIAQWVSLGNRAPTIRCIIVGDDPASHTYVRNK 385
+ S H ++IA I+ E+ +++ + P + +IVG S TYV K
Sbjct: 1 MASSSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTK 60
Query: 386 VEAAKFVGIDALTINRDSDITEEQLLSEIQNLNEDNNVDGI 508
+A VGI + + +++E L+S++ LN + +V GI
Sbjct: 61 RKACAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGI 101
>At2g38660.1 68415.m04748 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
precursor (C1-THF synthase) [Includes:
Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
Formyltetrahydrofolate synthetase (EC 6.3.4.3)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
NAD(P)-binding domain, PF00763: Tetrahydrofolate
dehydrogenase/cyclohydrolase, catalytic domain
Length = 352
Score = 78.6 bits (185), Expect = 4e-15
Identities = 37/85 (43%), Positives = 56/85 (65%), Gaps = 2/85 (2%)
Frame = +1
Query: 511 VQLPVPDTINERRVCDAIAPEKDIDGFHIINIGRLCV--DLPTIVPATALAVVEMLKRFN 684
VQLP+P +NE ++ + + EKD+DGFH +N+G L + P V T VE+L R
Sbjct: 157 VQLPLPQHLNESKILNMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTG 216
Query: 685 IDTFGRNAVVIGRSKNVGMPIAMML 759
++ G+NAVVIGRS VG+P++++L
Sbjct: 217 VEIAGKNAVVIGRSNIVGLPMSLLL 241
Score = 60.1 bits (139), Expect = 2e-09
Identities = 27/85 (31%), Positives = 49/85 (57%)
Frame = +2
Query: 254 IAMEIKKELKQKIAQWVSLGNRAPTIRCIIVGDDPASHTYVRNKVEAAKFVGIDALTINR 433
IA EI+ ++ ++ + + P + ++VG+ S TYVRNK++A + GI ++
Sbjct: 71 IAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKIKACEETGIKSVLAEL 130
Query: 434 DSDITEEQLLSEIQNLNEDNNVDGI 508
D TE Q++S ++ NED ++ GI
Sbjct: 131 PEDCTEGQIISVLRKFNEDTSIHGI 155
>At1g21200.1 68414.m02650 expressed protein
Length = 443
Score = 31.1 bits (67), Expect = 0.86
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = -3
Query: 714 HDRVATECVDVKSFQHFHYSQCCSRHNGR*IHTQPSDVDYVETIDIFFRS 565
+D+ + + S +H Y + CS HNG +H P D+ ++ + RS
Sbjct: 216 NDKEKDDVRKIMSSKHLFYEEMCSYHNGNRLHL-PHDLALQRSLQLALRS 264
>At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 575
Score = 28.7 bits (61), Expect = 4.6
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = -1
Query: 734 TFLERPITTALRPNVSMLNLFSISTTANAVA 642
T + PI+ L NVS LNLFS ST+++ +
Sbjct: 212 TVSQPPISNDLMNNVSSLNLFSSSTSSSTAS 242
>At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 498
Score = 28.7 bits (61), Expect = 4.6
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = -1
Query: 734 TFLERPITTALRPNVSMLNLFSISTTANAVA 642
T + PI+ L NVS LNLFS ST+++ +
Sbjct: 135 TVSQPPISNDLMNNVSSLNLFSSSTSSSTAS 165
>At5g64560.2 68418.m08114 magnesium transporter CorA-like family
protein (MRS2-2) weak similarity to SP|Q01926 RNA
splicing protein MRS2, mitochondrial precursor
{Saccharomyces cerevisiae}; contains Pfam profile
PF01544: CorA-like Mg2+ transporter protein
Length = 378
Score = 28.3 bits (60), Expect = 6.1
Identities = 20/85 (23%), Positives = 40/85 (47%)
Frame = +2
Query: 341 IVGDDPASHTYVRNKVEAAKFVGIDALTINRDSDITEEQLLSEIQNLNEDNNVDGISSNY 520
+V DP++ V+ K A + IDA + D+ + +++ +Q D + + N
Sbjct: 7 LVPADPSAVVTVKKKTPQASWALIDATGQSEPLDVDKYEIMHRVQIHARDLRI--LDPNL 64
Query: 521 LYLIQLMRGEFAMLLLLKKISMVST 595
Y ++ E A++L L+ I + T
Sbjct: 65 SYPSTILGRERAIVLNLEHIKAIIT 89
>At5g64560.1 68418.m08113 magnesium transporter CorA-like family
protein (MRS2-2) weak similarity to SP|Q01926 RNA
splicing protein MRS2, mitochondrial precursor
{Saccharomyces cerevisiae}; contains Pfam profile
PF01544: CorA-like Mg2+ transporter protein
Length = 394
Score = 28.3 bits (60), Expect = 6.1
Identities = 20/85 (23%), Positives = 40/85 (47%)
Frame = +2
Query: 341 IVGDDPASHTYVRNKVEAAKFVGIDALTINRDSDITEEQLLSEIQNLNEDNNVDGISSNY 520
+V DP++ V+ K A + IDA + D+ + +++ +Q D + + N
Sbjct: 7 LVPADPSAVVTVKKKTPQASWALIDATGQSEPLDVDKYEIMHRVQIHARDLRI--LDPNL 64
Query: 521 LYLIQLMRGEFAMLLLLKKISMVST 595
Y ++ E A++L L+ I + T
Sbjct: 65 SYPSTILGRERAIVLNLEHIKAIIT 89
>At2g29250.1 68415.m03554 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 623
Score = 28.3 bits (60), Expect = 6.1
Identities = 23/63 (36%), Positives = 26/63 (41%)
Frame = -1
Query: 704 LRPNVSMLNLFSISTTANAVAGTMVGRSTHSLPMLIMWKPSISFSGAIASQTLLSLIVSG 525
L PN S L F S G G H L +I PS+ FSGA+ S L S
Sbjct: 75 LNPNSSNLVSFPTSFVFAITPGP--GAPGHGLAFVI--SPSLDFSGALPSNYLGLFNTSN 130
Query: 524 TGN 516
GN
Sbjct: 131 NGN 133
>At3g45950.1 68416.m04972 splicing factor-related similar to step II
splicing factor SLU7 [Homo sapiens] GI:4249705
Length = 385
Score = 27.9 bits (59), Expect = 8.0
Identities = 17/70 (24%), Positives = 38/70 (54%)
Frame = +2
Query: 347 GDDPASHTYVRNKVEAAKFVGIDALTINRDSDITEEQLLSEIQNLNEDNNVDGISSNYLY 526
G DP+S+ +VR++ EA + R+ + E+QL+++++ N D + + +
Sbjct: 147 GYDPSSYCHVRDRHEAKENA--------REKYLNEQQLIAKLEEKNIDGEEEDLRVDEAK 198
Query: 527 LIQLMRGEFA 556
+ + M+ +FA
Sbjct: 199 IDESMQVDFA 208
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,808,182
Number of Sequences: 28952
Number of extensions: 350595
Number of successful extensions: 921
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 885
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 917
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1755792000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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