Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0216.Seq
         (443 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R...    60   7e-10
At4g28470.1 68417.m04073 26S proteasome regulatory subunit, puta...    53   1e-07
At1g64680.1 68414.m07332 expressed protein                             28   2.5  
At3g21540.1 68416.m02717 transducin family protein / WD-40 repea...    27   4.3  
At1g51100.1 68414.m05745 expressed protein                             26   10.0 

>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
           (RPN1) contains an APC-complex (cyclosome) and
           proteasome component repeat ( PS50248)
          Length = 891

 Score = 60.1 bits (139), Expect = 7e-10
 Identities = 28/65 (43%), Positives = 42/65 (64%)
 Frame = +3

Query: 249 PALSVIDVLNKYSHDSDNDVAYNAIFAMGLVGAGTNNXXXXXXXXXXXXYHGKSPVHLFM 428
           P ++V+D L++ SHD+D++VA +AI ++GL+GAGTNN            Y+ K    LF 
Sbjct: 684 PKVTVMDTLSRLSHDTDSEVAMSAIISLGLIGAGTNNARIAGMLRNLSSYYYKDMSLLFC 743

Query: 429 VRLAQ 443
           VR+AQ
Sbjct: 744 VRIAQ 748



 Score = 54.4 bits (125), Expect = 3e-08
 Identities = 25/53 (47%), Positives = 37/53 (69%)
 Frame = +1

Query: 94  QAVATLGVAVIALAEETGAEMCTRIFGQLGRYGEPVVRRAVPLAIALCSVSNP 252
           Q  A LG+A++A++EE G +M  R   ++ +YGE  +RRAVPLA+ L  +SNP
Sbjct: 632 QGPAVLGLAMVAMSEELGVDMEIRSLERMLQYGEQNIRRAVPLALGLLCISNP 684



 Score = 30.7 bits (66), Expect = 0.46
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = +3

Query: 258 SVIDVLNKYSHDSDNDVAYNAIFAMGLVGAGTNN 359
           S +  L+KY H +DN +   A+  +G+V  G  N
Sbjct: 429 SGLSQLDKYFHSNDNPIIAGALLGVGIVNCGIKN 462


>At4g28470.1 68417.m04073 26S proteasome regulatory subunit,
           putative contains Pfam domain PF01851:
           Proteasome/cyclosome repeat
          Length = 1103

 Score = 52.8 bits (121), Expect = 1e-07
 Identities = 24/53 (45%), Positives = 37/53 (69%)
 Frame = +1

Query: 94  QAVATLGVAVIALAEETGAEMCTRIFGQLGRYGEPVVRRAVPLAIALCSVSNP 252
           Q  A +G+A++A++EE G +M  R   ++ +YGE  +RRAVPLA+ L  +SNP
Sbjct: 704 QGPAVIGLAMVAMSEELGLDMEIRSLERVLQYGEQNIRRAVPLALGLLCISNP 756



 Score = 52.8 bits (121), Expect = 1e-07
 Identities = 25/61 (40%), Positives = 37/61 (60%)
 Frame = +3

Query: 249 PALSVIDVLNKYSHDSDNDVAYNAIFAMGLVGAGTNNXXXXXXXXXXXXYHGKSPVHLFM 428
           P ++V+D L++ SHD+D++VA  AI ++GL+GAGTNN            Y+ K    LF 
Sbjct: 756 PKVTVMDTLSRLSHDTDSEVAMAAIISLGLIGAGTNNARIAGMLRNLSSYYYKDASLLFC 815

Query: 429 V 431
           V
Sbjct: 816 V 816



 Score = 31.1 bits (67), Expect = 0.35
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +3

Query: 273 LNKYSHDSDNDVAYNAIFAMGLVGAGTNN 359
           L+KY H +DN V   A+  +G+V  G  N
Sbjct: 477 LDKYFHSNDNPVVAGALLGVGIVNCGIKN 505


>At1g64680.1 68414.m07332 expressed protein
          Length = 250

 Score = 28.3 bits (60), Expect = 2.5
 Identities = 14/35 (40%), Positives = 17/35 (48%)
 Frame = +1

Query: 157 CTRIFGQLGRYGEPVVRRAVPLAIALCSVSNPHSP 261
           C  I+GQ     E  V    P    +CS+SNP SP
Sbjct: 209 CEMIYGQAPPAFEEDVATKQPCLADICSMSNPSSP 243


>At3g21540.1 68416.m02717 transducin family protein / WD-40 repeat
           family protein contains Pfam profile: PF00400 WD domain,
           G-beta repeat (10 copies); similar to WD-repeat protein
           3 (SP:Q9UNX4) [Homo sapiens]
          Length = 955

 Score = 27.5 bits (58), Expect = 4.3
 Identities = 13/27 (48%), Positives = 17/27 (62%)
 Frame = -2

Query: 310 ATSLSESWEYLLSTSMTESAGWTPSTG 230
           + +LSE    L+STS +E   W PSTG
Sbjct: 411 SVTLSEDNTLLMSTSHSEVKIWNPSTG 437


>At1g51100.1 68414.m05745 expressed protein
          Length = 211

 Score = 26.2 bits (55), Expect = 10.0
 Identities = 10/38 (26%), Positives = 21/38 (55%)
 Frame = -1

Query: 203 TTGSPYLPSCPKMRVHISAPVSSANAMTATPRVATACT 90
           +T +  LPS     +HI+ P+ + + +  T + +T C+
Sbjct: 3   STSTLLLPSLSSKNLHIAVPIRTNSFVRRTTKFSTKCS 40


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,003,067
Number of Sequences: 28952
Number of extensions: 110284
Number of successful extensions: 339
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 332
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 339
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)