BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0215.Seq (688 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 69 5e-13 SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 28 1.5 SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 28 1.5 SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 27 2.5 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 27 3.4 SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizo... 25 7.8 SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 25 7.8 SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 25 7.8 SPBC28F2.11 |||INO80 complex subunit |Schizosaccharomyces pombe|... 25 7.8 >SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1009 Score = 69.3 bits (162), Expect = 5e-13 Identities = 31/71 (43%), Positives = 42/71 (59%), Gaps = 1/71 (1%) Frame = +1 Query: 7 TAAVSVNANRLKSSTAAEPFLNGSSSAYVETMYNAWLADPNSVHASWDAFFRNA-TNGAQ 183 T S+ NR ++ + FL G ++ YV+ MY+AW DPNSVHASW A+F+N G Sbjct: 28 TCLSSLQQNRTFATQPTDDFLTGGAADYVDEMYDAWKKDPNSVHASWQAYFKNVQERGVS 87 Query: 184 PGAAYTPPPNL 216 P A+ PP L Sbjct: 88 PSKAFQAPPLL 98 Score = 47.6 bits (108), Expect = 2e-06 Identities = 36/118 (30%), Positives = 59/118 (50%), Gaps = 3/118 (2%) Frame = +3 Query: 324 HLAVQAIIRSYQARGHLAADVDPLGITTATLPELGMRAPSSELIMRKYFNFDEADMDRVF 503 ++ VQ ++R+YQ+RGH A +DPLGI + P SEL + +++ F E+D++R Sbjct: 125 YMKVQLLVRAYQSRGHHLAKLDPLGI------NVNHNRP-SELTL-EHYGFTESDLNRTI 176 Query: 504 KL-PQLRLSAXXXXXXXXXXF*IV--WSKRTATNIGIEFMFINSLEQCNWIRQRMEPP 668 L P + + IV K + +EF I+S ++ NWI +E P Sbjct: 177 HLGPGILPNFREAGRKTMTLREIVETCEKIYCGSFAVEFTHISSRKRSNWILSHLETP 234 >SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase Fab1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1932 Score = 27.9 bits (59), Expect = 1.5 Identities = 23/71 (32%), Positives = 31/71 (43%) Frame = -1 Query: 574 QTIQNFSQRERFFFFADKRS*GSLNTLSMSASSKLKYFLMMSSELGARMPSSGSVAVVMP 395 QT N SQR+ + KR SAS+ + F+ SS G MP S S++ + Sbjct: 1336 QTYPNISQRQGVNMLSHKRK---------SASTSDRRFVNASSTSGMNMPISSSISAKIS 1386 Query: 394 SGSTSAAR*PR 362 S S PR Sbjct: 1387 SIQNSTKYSPR 1397 >SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 732 Score = 27.9 bits (59), Expect = 1.5 Identities = 16/45 (35%), Positives = 26/45 (57%) Frame = +1 Query: 445 PSSS*GNISISMKPTWTGYSNSLNYVYRRKRKSAPVERNSESFGA 579 PSSS +I+ +PT + +N+ ++ R+ R SA R+ E F A Sbjct: 631 PSSSINLANITSRPTNSSAANTPSWGVRKARASALNARSEEDFPA 675 >SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Schizosaccharomyces pombe|chr 1|||Manual Length = 794 Score = 27.1 bits (57), Expect = 2.5 Identities = 16/57 (28%), Positives = 29/57 (50%) Frame = -1 Query: 589 AVRLLQTIQNFSQRERFFFFADKRS*GSLNTLSMSASSKLKYFLMMSSELGARMPSS 419 A+ LLQ S +RF + ++ G L LS +++ +K +M + G ++ SS Sbjct: 174 AISLLQAYATISTNKRFMVYHQTKNSGKLLQLSTNSNKDMKLNIM--NVFGTKVSSS 228 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/55 (23%), Positives = 29/55 (52%) Frame = +1 Query: 463 NISISMKPTWTGYSNSLNYVYRRKRKSAPVERNSESFGASVLQQTSVLSSCS*IP 627 ++S K + + S+ V +R +++ +++FG+S+ + VLS C +P Sbjct: 1094 DVSNDRKSSLSSVSDKDAAVLQRMGAQLTLQQMAQNFGSSLFSRVPVLSQCLFVP 1148 >SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizosaccharomyces pombe|chr 3|||Manual Length = 650 Score = 25.4 bits (53), Expect = 7.8 Identities = 20/78 (25%), Positives = 31/78 (39%), Gaps = 1/78 (1%) Frame = +1 Query: 1 PQTAAVSVNANRLKSSTAAEPFLNGSSSAYVETM-YNAWLADPNSVHASWDAFFRNATNG 177 P + + N N + + + NG++S+ V Y ++ S F T G Sbjct: 361 PMASPMLPNVNSMPYLSNGDHNNNGNTSSPVSRFSYGQHTSNVPSTQKLPSNF--RVTQG 418 Query: 178 AQPGAAYTPPPNLAPYNK 231 A P Y PPP + P K Sbjct: 419 APPSHTYGPPPPVQPKPK 436 >SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.4 bits (53), Expect = 7.8 Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 1/49 (2%) Frame = +1 Query: 61 PFLNGSSSAYVETMYNAWLAD-PNSVHASWDAFFRNATNGAQPGAAYTP 204 P N + SA + + ++D PN H DAF ++ + QP +A P Sbjct: 9 PVGNETKSAALNALPKIKISDSPNRHHNLVDAFMQSPSYSTQPKSAVEP 57 >SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2100 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = -1 Query: 505 LNTLSMSASSKLKYFLMMSSELGARMPSS 419 ++T++ S SK +YFL+ + R PSS Sbjct: 1966 ISTMNRSCDSKCRYFLLTAIANQLRYPSS 1994 >SPBC28F2.11 |||INO80 complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 310 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = +2 Query: 44 APPRRNPSSMAPVQLTSRPCTMHGSPTQTPYTR 142 +P + P+++APV+ TS T + TP R Sbjct: 77 SPKKATPAAVAPVEATSAVDTSEAVASMTPNKR 109 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,006,769 Number of Sequences: 5004 Number of extensions: 62217 Number of successful extensions: 222 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 207 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 220 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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