Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0185X.Seq
(623 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 174 7e-45
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 105 4e-24
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 100 3e-22
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 97 2e-21
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 66 3e-12
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 64 2e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 56 5e-09
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 39 5e-04
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 30 0.24
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 28 1.3
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 27 2.2
SPCC306.02c |||Rab GTPase binding |Schizosaccharomyces pombe|chr... 27 2.9
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 27 2.9
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.9
SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr... 27 2.9
SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos... 26 5.1
SPBC3H7.04 |||mitochondrial ribosomal protein subunit S26|Schizo... 25 8.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 174 bits (424), Expect = 7e-45
Identities = 78/88 (88%), Positives = 84/88 (95%)
Frame = +3
Query: 252 HNWDDMEKIWHHTFYNELRVAPEEDPVLVTEAPLNPKANREKMTQIMFETFNSPAMYVAI 431
+NWDDMEKIWHHTFYNELRVAPEE P L+TEAPLNPK+NREKMTQI+FETFN+PA YVAI
Sbjct: 77 NNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAI 136
Query: 432 QAVLSLYASGRTTGIVLDSGDGVSHTYP 515
QAVLSLYASGRTTGIVLDSGDGV+HT P
Sbjct: 137 QAVLSLYASGRTTGIVLDSGDGVTHTVP 164
Score = 150 bits (363), Expect = 2e-37
Identities = 66/75 (88%), Positives = 73/75 (97%)
Frame = +1
Query: 28 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 207
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 208 RRILTLKYPIEHGII 252
R ILTLKYPIEHGI+
Sbjct: 62 RGILTLKYPIEHGIV 76
Score = 76.2 bits (179), Expect = 4e-15
Identities = 33/38 (86%), Positives = 36/38 (94%)
Frame = +2
Query: 509 VPIYEGYALPHAILRLDLAGRDFTDYLMKILTEKGYSF 622
VPIYEGYALPHAI+RLDLAGRD TDYLMKIL E+GY+F
Sbjct: 163 VPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTF 200
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 105 bits (253), Expect = 4e-24
Identities = 44/87 (50%), Positives = 66/87 (75%)
Frame = +3
Query: 255 NWDDMEKIWHHTFYNELRVAPEEDPVLVTEAPLNPKANREKMTQIMFETFNSPAMYVAIQ 434
++++M ++W +TF+ +L++ P +L+TE P+NP ANREKM + MFE + +YVAIQ
Sbjct: 78 DFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQ 137
Query: 435 AVLSLYASGRTTGIVLDSGDGVSHTYP 515
AVLSLYA G ++G+V+DSGDGV+H P
Sbjct: 138 AVLSLYAQGLSSGVVVDSGDGVTHIVP 164
Score = 72.5 bits (170), Expect = 4e-14
Identities = 37/73 (50%), Positives = 50/73 (68%), Gaps = 2/73 (2%)
Frame = +1
Query: 40 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRR 213
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 214 ILTLKYPIEHGII 252
+L +KYP+E+GII
Sbjct: 64 LLQVKYPMENGII 76
Score = 49.6 bits (113), Expect = 4e-07
Identities = 21/38 (55%), Positives = 27/38 (71%)
Frame = +2
Query: 509 VPIYEGYALPHAILRLDLAGRDFTDYLMKILTEKGYSF 622
VP+YE L H + RLD+AGRD T YL+ +L KGY+F
Sbjct: 163 VPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAF 200
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 99.5 bits (237), Expect = 3e-22
Identities = 44/88 (50%), Positives = 63/88 (71%), Gaps = 1/88 (1%)
Frame = +3
Query: 255 NWDDMEKIWHHTFYNE-LRVAPEEDPVLVTEAPLNPKANREKMTQIMFETFNSPAMYVAI 431
NW DME+IW + + ++ L PEE P+L+TE PL N+EK+ + +ET N PA+ ++
Sbjct: 82 NWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSL 141
Query: 432 QAVLSLYASGRTTGIVLDSGDGVSHTYP 515
Q VL+LYAS RTTGIVL+ GDG++H+ P
Sbjct: 142 QPVLALYASARTTGIVLECGDGLTHSVP 169
Score = 85.0 bits (201), Expect = 8e-18
Identities = 39/73 (53%), Positives = 51/73 (69%)
Frame = +1
Query: 34 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRR 213
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ R
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 214 ILTLKYPIEHGII 252
+L ++ PIE GII
Sbjct: 68 LLKIQRPIERGII 80
Score = 42.3 bits (95), Expect = 5e-05
Identities = 17/36 (47%), Positives = 26/36 (72%)
Frame = +2
Query: 509 VPIYEGYALPHAILRLDLAGRDFTDYLMKILTEKGY 616
VPIY+G+++P AI + ++ GRD TDYL L + G+
Sbjct: 168 VPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGH 203
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 96.7 bits (230), Expect = 2e-21
Identities = 48/105 (45%), Positives = 63/105 (60%), Gaps = 8/105 (7%)
Frame = +3
Query: 225 EVPHRARYHHNWDDMEKIWHHTFYNELRVAPEEDPVLVTEAPLNPKANREKMTQIMFETF 404
+ P R NWD ME+ W + + LR PE+ L+TE PLNP NRE +IMFE+F
Sbjct: 91 DYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESF 150
Query: 405 NSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTYP 515
N +Y+A+QAVL+L AS + TG V+DSGDGV+H P
Sbjct: 151 NCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIP 195
Score = 42.7 bits (96), Expect = 4e-05
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +1
Query: 46 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 195
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ +
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
Score = 32.3 bits (70), Expect = 0.059
Identities = 12/34 (35%), Positives = 22/34 (64%)
Frame = +2
Query: 509 VPIYEGYALPHAILRLDLAGRDFTDYLMKILTEK 610
+P+ EGY + +I + LAGRD T ++ +L ++
Sbjct: 194 IPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDR 227
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 66.5 bits (155), Expect = 3e-12
Identities = 33/94 (35%), Positives = 50/94 (53%), Gaps = 2/94 (2%)
Frame = +3
Query: 216 PHSEVPHRAR--YHHNWDDMEKIWHHTFYNELRVAPEEDPVLVTEAPLNPKANREKMTQI 389
P E+ + R + NWD +W + +L+ P E P+L+TE NP NR K +
Sbjct: 61 PGMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120
Query: 390 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 491
MFE+ PA Y+A Q + +ASG+ T ++D G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIG 154
Score = 40.7 bits (91), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +1
Query: 31 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 132
D+V+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 64.1 bits (149), Expect = 2e-11
Identities = 32/89 (35%), Positives = 53/89 (59%), Gaps = 2/89 (2%)
Frame = +3
Query: 255 NWDDMEKIWHHTFYNELRVAPEEDPVLVTEAPLNPKANREKMTQIMFETFNSPAMYVAIQ 434
NWD ME++ +TF +L + E P+ +TE NP R MT+++FE +N+P++ I
Sbjct: 94 NWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGID 152
Query: 435 AVLSLYASGR--TTGIVLDSGDGVSHTYP 515
+ S Y + + ++GIVL+ G+ SH P
Sbjct: 153 GLFSFYHNTKPSSSGIVLNLGNAASHVIP 181
Score = 36.7 bits (81), Expect = 0.003
Identities = 17/42 (40%), Positives = 27/42 (64%)
Frame = +1
Query: 16 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 141
F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 55.6 bits (128), Expect = 5e-09
Identities = 26/86 (30%), Positives = 48/86 (55%), Gaps = 1/86 (1%)
Frame = +3
Query: 255 NWDDMEKIWHHTFYNELRVAPEEDPVLVTEAPLNPKANREKMTQIMFETFNSPAMYVAIQ 434
+W+ W +L+V P E +++TE NP++ R+++ + FE + PA Y+ Q
Sbjct: 79 DWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQ 138
Query: 435 AVLSLYASGRTTGIVLDSG-DGVSHT 509
AV +A+ ++T +++D G D S T
Sbjct: 139 AVCVAFANSKSTALIVDIGSDNASVT 164
Score = 38.3 bits (85), Expect = 9e-04
Identities = 27/88 (30%), Positives = 46/88 (52%), Gaps = 6/88 (6%)
Frame = +1
Query: 31 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 210
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 211 RI--LTLKYPIEHGII----TTGMTWRR 276
I + +K +GII +T TW R
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWER 89
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 39.1 bits (87), Expect = 5e-04
Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 4/83 (4%)
Frame = +3
Query: 255 NWDDMEKIWHHTFYNELRVAPEED----PVLVTEAPLNPKANREKMTQIMFETFNSPAMY 422
+W+ ++ W H Y+ L P + PV + +RE TQ FE P
Sbjct: 121 DWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFEECQVPGFT 179
Query: 423 VAIQAVLSLYASGRTTGIVLDSG 491
+A + ++ LYA G G+V+D G
Sbjct: 180 IAYEPLMGLYAIGILHGLVIDIG 202
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 30.3 bits (65), Expect = 0.24
Identities = 15/32 (46%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
Frame = +3
Query: 315 PEEDPVLVTE-APLNPKANREKMTQIMFETFN 407
PEED V E APL+ + +KM +++FET+N
Sbjct: 174 PEEDFFRVLETAPLHAEEVSKKMKELLFETYN 205
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 27.9 bits (59), Expect = 1.3
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +1
Query: 91 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRRILTLKYP 234
G+ PRA F ++ P H G+++ M KD G+E S + + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 27.1 bits (57), Expect = 2.2
Identities = 9/37 (24%), Positives = 23/37 (62%)
Frame = +2
Query: 509 VPIYEGYALPHAILRLDLAGRDFTDYLMKILTEKGYS 619
+P+ + A+ R+D+ G+ T+YL ++++ + Y+
Sbjct: 171 IPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYN 207
>SPCC306.02c |||Rab GTPase binding |Schizosaccharomyces pombe|chr
3|||Manual
Length = 171
Score = 26.6 bits (56), Expect = 2.9
Identities = 17/57 (29%), Positives = 28/57 (49%)
Frame = +3
Query: 357 PKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTYPSTKV 527
P+ E ++I F + Y+AI A+L +YA R +++ G GV Y K+
Sbjct: 44 PRNFSEAQSRISFNFSRFSSNYLAIIAMLVIYALIRNPLLLIVIGIGVGGVYGIRKL 100
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 26.6 bits (56), Expect = 2.9
Identities = 24/92 (26%), Positives = 46/92 (50%), Gaps = 4/92 (4%)
Frame = +3
Query: 306 RVAPE----EDPVLVTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG 473
+VAP+ D + VT L+P +R+ + + + ++ + + L+L +GR G
Sbjct: 249 KVAPDGSNPADGITVTGNLLSPPNSRDLQVEKL--SVSAWGKLLIKDSELNLI-NGRRYG 305
Query: 474 IVLDSGDGVSHTYPSTKVTLCPTPSSVWTWLV 569
++ +G G S + L PTPSS+ +L+
Sbjct: 306 LIAPNGSGKSTLLHAIACGLIPTPSSLDFYLL 337
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 2.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 516 STKVTLCPTPSSVWTW 563
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 2.9
Identities = 7/15 (46%), Positives = 10/15 (66%)
Frame = +3
Query: 219 HSEVPHRARYHHNWD 263
H+++ H YHH WD
Sbjct: 364 HTKLMHELEYHHGWD 378
>SPBP35G2.05c |cki2||serine/threonine protein kinase
Cki2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 435
Score = 25.8 bits (54), Expect = 5.1
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = -2
Query: 595 LHEVVGKVATSQVQTEDGVGQSVTFVDGYVWDTPSPESSTIPVVRPEAY 449
L E+ V + T DGV + DG W++ S S + + R + Y
Sbjct: 274 LQELFDDVLRANGDTNDGVYDWMLLNDGKGWESSSSHFSVVAMKRRKNY 322
>SPBC3H7.04 |||mitochondrial ribosomal protein subunit
S26|Schizosaccharomyces pombe|chr 2|||Manual
Length = 220
Score = 25.0 bits (52), Expect = 8.9
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = -2
Query: 541 VGQSVTFVDGYVWDTPSPESSTIPVVRPEAYSESTAW 431
VG SV F DG++W SPE S ++ S + W
Sbjct: 123 VGNSV-FGDGWLWLVYSPEKSLFSLLCTYNASNAFLW 158
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,427,236
Number of Sequences: 5004
Number of extensions: 48704
Number of successful extensions: 201
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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