Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0184.Seq
(710 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 54 2e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 44 2e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 43 5e-05
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 31 0.21
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.50
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 28 1.5
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.6
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.6
SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces... 26 6.1
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 6.1
SPAC16C9.01c ||SPAC4G8.14c|carbohydrate kinase |Schizosaccharomy... 26 6.1
SPCC1494.01 ||SPCC965.15|iron/ascorbate oxidoreductase family|Sc... 25 8.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 54.0 bits (124), Expect = 2e-08
Identities = 27/75 (36%), Positives = 44/75 (58%)
Frame = +2
Query: 248 SKEDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 427
+++ KD E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 75 ARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DT 132
Query: 428 DDDGMIPYAAFLKKV 472
D DG+I Y F + +
Sbjct: 133 DGDGVINYEEFSRVI 147
Score = 31.1 bits (67), Expect = 0.16
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +3
Query: 30 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI 173
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAEL 50
Score = 29.1 bits (62), Expect = 0.66
Identities = 15/61 (24%), Positives = 32/61 (52%)
Frame = +2
Query: 281 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 460
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 461 L 463
L
Sbjct: 71 L 71
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 44.0 bits (99), Expect = 2e-05
Identities = 23/70 (32%), Positives = 42/70 (60%)
Frame = +2
Query: 269 GAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIP 448
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+
Sbjct: 74 GDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVN 130
Query: 449 YAAFLKKVMA 478
Y F++ ++A
Sbjct: 131 YHDFVQMILA 140
Score = 42.3 bits (95), Expect = 7e-05
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = +3
Query: 72 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI 173
AFS++D G G+I ++GDLLRA NPTLA I
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI 44
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 42.7 bits (96), Expect = 5e-05
Identities = 21/77 (27%), Positives = 46/77 (59%)
Frame = +2
Query: 248 SKEDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 427
S + ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP
Sbjct: 68 SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP- 125
Query: 428 DDDGMIPYAAFLKKVMA 478
+ G Y F++++MA
Sbjct: 126 TNSGSFDYYDFVQRIMA 142
Score = 30.7 bits (66), Expect = 0.21
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = +2
Query: 278 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 457
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 30.7 bits (66), Expect = 0.21
Identities = 21/80 (26%), Positives = 38/80 (47%)
Frame = +1
Query: 34 ATSARTTLKGRLSPSQSTTLKAKARSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSC 213
++S+ ++ S S S++ + + S S+ + S S+ + S +S V + S
Sbjct: 157 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSS 216
Query: 214 SHSKSSFPSTAKQRRQRPGS 273
SHS SS S++ RP S
Sbjct: 217 SHSSSSSSSSSSSSSSRPSS 236
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.5 bits (63), Expect = 0.50
Identities = 18/74 (24%), Positives = 34/74 (45%)
Frame = +2
Query: 191 KEEGREAAHTRRVPSHLQPSKEDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALG 370
K R ++ T P + + + +D E KL+D +++ + EL + ALG
Sbjct: 8 KRRSRASSPTPARLGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
Query: 371 EKLDDSEVAEVTKD 412
+ SEV ++ +D
Sbjct: 68 FNAEKSEVLKILRD 81
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +3
Query: 39 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 155
+ ++ +E AF ++D + GKI NL + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
Score = 26.6 bits (56), Expect = 3.5
Identities = 17/66 (25%), Positives = 29/66 (43%)
Frame = +2
Query: 278 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 457
E+ +L+D +E G + L L E +DD E+ + ++ D DG I
Sbjct: 110 EEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLDQDGEINEQE 167
Query: 458 FLKKVM 475
F+ +M
Sbjct: 168 FIAIMM 173
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.9 bits (59), Expect = 1.5
Identities = 20/71 (28%), Positives = 33/71 (46%)
Frame = +1
Query: 70 SPSQSTTLKAKARSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHSKSSFPSTAK 249
SP Q T+ MP +A + ++P + SRN+ + RR+ + S S PS
Sbjct: 536 SPVQPTSYNRTLPPMPEVVAYKGDE--ESP--RVSRNTSLARRKPLPDTESHSPSPSATS 591
Query: 250 QRRQRPGSV*R 282
++ P S+ R
Sbjct: 592 SIKKNPSSIFR 602
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 2.6
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +3
Query: 30 MSDLSKNDVER-ASFAFSIYDFEGKGKID 113
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 26.2 bits (55), Expect = 4.6
Identities = 20/71 (28%), Positives = 36/71 (50%)
Frame = +1
Query: 49 TTLKGRLSPSQSTTLKAKARSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHSKS 228
++L +L+P T KAK+ S+ S S S+ + W + V++ A S S++
Sbjct: 348 SSLSRKLAPPFLRTHKAKSNSLFSMKRPSSSSSSLSGSWHGDTENSVKQSLA---SPSEA 404
Query: 229 SFPSTAKQRRQ 261
S P+ +K R+
Sbjct: 405 SLPNLSKYSRK 415
>SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 897
Score = 25.8 bits (54), Expect = 6.1
Identities = 15/55 (27%), Positives = 24/55 (43%)
Frame = +1
Query: 109 SMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHSKSSFPSTAKQRRQRPGS 273
++P+T+ I+ ST QPS Q+ + + SS+P Q P S
Sbjct: 244 NLPATVPITQAASTANAFQQPSNQFQTQKLPSGLDTRPVSSYPDELTQLESNPDS 298
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 6.1
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +2
Query: 365 LGEKLDDSEVAEVTKDCMDPED 430
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPAC16C9.01c ||SPAC4G8.14c|carbohydrate kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 361
Score = 25.8 bits (54), Expect = 6.1
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 529 CDECAMAGTLKDRSPGLKEPAPEA 600
C+E A G LK+R + EP PE+
Sbjct: 144 CEELAKYGNLKNRPKIVWEPTPES 167
>SPCC1494.01 ||SPCC965.15|iron/ascorbate oxidoreductase
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 321
Score = 25.4 bits (53), Expect = 8.1
Identities = 9/34 (26%), Positives = 20/34 (58%)
Frame = +2
Query: 227 VPSHLQPSKEDKDQGAYEDFLECLKLYDKNENGL 328
+P ++ ++D+D GA+ D+ L+ ++ GL
Sbjct: 170 IPEGVERREDDEDAGAHSDYGSITLLFQRDAAGL 203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,507,923
Number of Sequences: 5004
Number of extensions: 43377
Number of successful extensions: 138
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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