Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0181.Seq
(804 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q7KT91 Cluster: Non-lysosomal glucosylceramidase; n=5; ... 62 1e-08
UniRef50_UPI00015B5FEB Cluster: PREDICTED: similar to CG33090-PB... 61 4e-08
UniRef50_UPI0000D576AC Cluster: PREDICTED: similar to CG33090-PB... 59 1e-07
UniRef50_UPI0000ECBFCA Cluster: Non-lysosomal glucosylceramidase... 53 7e-06
UniRef50_Q9LRN1 Cluster: Emb|CAB39630.1; n=3; Magnoliophyta|Rep:... 52 2e-05
UniRef50_Q4TD68 Cluster: Chromosome undetermined SCAF6472, whole... 52 2e-05
UniRef50_Q7XDG7 Cluster: Expressed protein; n=5; Oryza sativa|Re... 51 4e-05
UniRef50_Q0D6T0 Cluster: Os07g0444000 protein; n=2; Oryza sativa... 50 5e-05
UniRef50_Q9HCG7 Cluster: Non-lysosomal glucosylceramidase; n=27;... 48 4e-04
UniRef50_A5BBB7 Cluster: Putative uncharacterized protein; n=1; ... 47 5e-04
UniRef50_A7NXU8 Cluster: Chromosome chr5 scaffold_2, whole genom... 46 8e-04
UniRef50_Q8LPR0 Cluster: AT4g10060/T5L19_190; n=3; Arabidopsis t... 46 0.001
UniRef50_A3BJA3 Cluster: Putative uncharacterized protein; n=1; ... 46 0.001
UniRef50_A0MNZ0 Cluster: NADPH oxidoreductase; n=1; Bombyx mori|... 43 0.008
UniRef50_Q9LQ24 Cluster: F14M2.16 protein; n=1; Arabidopsis thal... 43 0.010
UniRef50_Q9LTY0 Cluster: Arabidopsis thaliana genomic DNA, chrom... 41 0.042
UniRef50_Q0LJ08 Cluster: Putative uncharacterized protein; n=1; ... 40 0.073
UniRef50_A7QC94 Cluster: Chromosome undetermined scaffold_77, wh... 40 0.073
UniRef50_Q8WQB2 Cluster: Putative uncharacterized protein; n=4; ... 40 0.097
UniRef50_Q1IR11 Cluster: Putative uncharacterized protein precur... 38 0.22
UniRef50_Q7NHS5 Cluster: Gll2460 protein; n=1; Gloeobacter viola... 36 0.90
UniRef50_A0JP97 Cluster: LOC100036642 protein; n=7; Eumetazoa|Re... 34 3.6
UniRef50_Q5LPC3 Cluster: Putative uncharacterized protein; n=1; ... 34 3.6
UniRef50_Q52UP0 Cluster: DNA polymerase; n=24; root|Rep: DNA pol... 34 4.8
UniRef50_O01893 Cluster: Putative uncharacterized protein R08F11... 34 4.8
>UniRef50_Q7KT91 Cluster: Non-lysosomal glucosylceramidase; n=5;
Diptera|Rep: Non-lysosomal glucosylceramidase -
Drosophila melanogaster (Fruit fly)
Length = 948
Score = 62.5 bits (145), Expect = 1e-08
Identities = 28/74 (37%), Positives = 41/74 (55%)
Frame = +1
Query: 79 FLRASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGRGTCTPSRCRARG 258
+L++ G+ ++P+ NVR ALK I+DNNVM F G +GA NGF+A P
Sbjct: 805 YLKSCGFDYEIYPKENVRTALKRIYDNNVMGFHEGNIGAANGFIANASEPTKPGHVDNSN 864
Query: 259 LDRSHVRPGLAHGL 300
+ V PG+ + L
Sbjct: 865 IQAEEVWPGVVYAL 878
>UniRef50_UPI00015B5FEB Cluster: PREDICTED: similar to CG33090-PB;
n=1; Nasonia vitripennis|Rep: PREDICTED: similar to
CG33090-PB - Nasonia vitripennis
Length = 826
Score = 60.9 bits (141), Expect = 4e-08
Identities = 27/46 (58%), Positives = 34/46 (73%)
Frame = +1
Query: 82 LRASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEG 219
LRA G++ VFPQ VR +L+T+F NNVMK+K G+ GAVNGF G
Sbjct: 674 LRACGFSYEVFPQDRVRSSLETVFQNNVMKYKNGQQGAVNGFSPTG 719
>UniRef50_UPI0000D576AC Cluster: PREDICTED: similar to CG33090-PB;
n=1; Tribolium castaneum|Rep: PREDICTED: similar to
CG33090-PB - Tribolium castaneum
Length = 812
Score = 59.3 bits (137), Expect = 1e-07
Identities = 28/67 (41%), Positives = 40/67 (59%), Gaps = 1/67 (1%)
Frame = +1
Query: 13 CVTYFCCDFVFK-VAIDVFSSTRFLRASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKM 189
C + C D + + D +LR+ G+ VFPQ V+ +LKTI++NNV F G+M
Sbjct: 654 CYNFDCSDKECRSIMADQLCGQWYLRSCGFNYEVFPQDRVKTSLKTIYENNVQSFCDGRM 713
Query: 190 GAVNGFV 210
GAVNGF+
Sbjct: 714 GAVNGFI 720
>UniRef50_UPI0000ECBFCA Cluster: Non-lysosomal glucosylceramidase
(EC 3.2.1.45) (NLGase) (Glucosylceramidase 2)
(Beta-glucocerebrosidase 2) (Beta-glucosidase 2).; n=1;
Gallus gallus|Rep: Non-lysosomal glucosylceramidase (EC
3.2.1.45) (NLGase) (Glucosylceramidase 2)
(Beta-glucocerebrosidase 2) (Beta-glucosidase 2). -
Gallus gallus
Length = 687
Score = 53.2 bits (122), Expect = 7e-06
Identities = 23/37 (62%), Positives = 29/37 (78%)
Frame = +1
Query: 109 VFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEG 219
VFP++++ ALKTIF+ NVM F GGKMGAVNG +G
Sbjct: 549 VFPKSHIVSALKTIFEKNVMSFAGGKMGAVNGMRPDG 585
>UniRef50_Q9LRN1 Cluster: Emb|CAB39630.1; n=3; Magnoliophyta|Rep:
Emb|CAB39630.1 - Arabidopsis thaliana (Mouse-ear cress)
Length = 937
Score = 52.0 bits (119), Expect = 2e-05
Identities = 26/54 (48%), Positives = 34/54 (62%), Gaps = 2/54 (3%)
Frame = +1
Query: 88 ASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR--GTCTPSR 243
AS +F ++ +R ++ IFD NVMK KGGKMGAVNG +G+ TC SR
Sbjct: 760 ASSGLPPLFEESKIRSTMQKIFDFNVMKTKGGKMGAVNGMHPDGKVDDTCMQSR 813
>UniRef50_Q4TD68 Cluster: Chromosome undetermined SCAF6472, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF6472,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 239
Score = 51.6 bits (118), Expect = 2e-05
Identities = 23/40 (57%), Positives = 28/40 (70%)
Frame = +1
Query: 100 TEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEG 219
T FP+ ++ ALK+IFD NVM F GG+MGAVNG EG
Sbjct: 172 TRQAFPKEKIQTALKSIFDLNVMSFAGGQMGAVNGMRPEG 211
>UniRef50_Q7XDG7 Cluster: Expressed protein; n=5; Oryza sativa|Rep:
Expressed protein - Oryza sativa subsp. japonica (Rice)
Length = 974
Score = 50.8 bits (116), Expect = 4e-05
Identities = 29/73 (39%), Positives = 39/73 (53%), Gaps = 2/73 (2%)
Frame = +1
Query: 88 ASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR--GTCTPSRCRARGL 261
AS +F + +R AL+ IF+ NVMK KGG++GAVNG G+ TC SR G+
Sbjct: 795 ASSGLPPLFDENKIRSALQKIFEFNVMKVKGGRLGAVNGMTPNGKVDETCMQSREIWTGV 854
Query: 262 DRSHVRPGLAHGL 300
L HG+
Sbjct: 855 TYGVAANMLLHGM 867
>UniRef50_Q0D6T0 Cluster: Os07g0444000 protein; n=2; Oryza
sativa|Rep: Os07g0444000 protein - Oryza sativa subsp.
japonica (Rice)
Length = 500
Score = 50.4 bits (115), Expect = 5e-05
Identities = 29/73 (39%), Positives = 39/73 (53%), Gaps = 2/73 (2%)
Frame = +1
Query: 88 ASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR--GTCTPSRCRARGL 261
AS +F + ++ L+ IFD NVM+ KGG+MGAVNG G+ TC SR G+
Sbjct: 321 ASSGLPPLFDEGRIKCTLQKIFDYNVMRVKGGRMGAVNGMHPNGKVDETCMQSREIWTGV 380
Query: 262 DRSHVRPGLAHGL 300
S L HG+
Sbjct: 381 TYSLAATMLLHGM 393
>UniRef50_Q9HCG7 Cluster: Non-lysosomal glucosylceramidase; n=27;
Eumetazoa|Rep: Non-lysosomal glucosylceramidase - Homo
sapiens (Human)
Length = 927
Score = 47.6 bits (108), Expect = 4e-04
Identities = 26/50 (52%), Positives = 31/50 (62%), Gaps = 3/50 (6%)
Frame = +1
Query: 79 FLRASGWTEA---VFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEG 219
FL+A G E VFP +V +AL+TIF+ NV F GG MGAVNG G
Sbjct: 759 FLKACGLGEGDTEVFPTQHVVRALQTIFELNVQAFAGGAMGAVNGMQPHG 808
>UniRef50_A5BBB7 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 900
Score = 47.2 bits (107), Expect = 5e-04
Identities = 24/54 (44%), Positives = 32/54 (59%), Gaps = 2/54 (3%)
Frame = +1
Query: 88 ASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR--GTCTPSR 243
AS ++F ++ +L I+D NVMK KGGKMGAVNG G+ +C SR
Sbjct: 685 ASSGLPSLFDDCKIKSSLHKIYDFNVMKVKGGKMGAVNGMHPNGKVDESCMQSR 738
>UniRef50_A7NXU8 Cluster: Chromosome chr5 scaffold_2, whole genome
shotgun sequence; n=1; Vitis vinifera|Rep: Chromosome
chr5 scaffold_2, whole genome shotgun sequence - Vitis
vinifera (Grape)
Length = 903
Score = 46.4 bits (105), Expect = 8e-04
Identities = 24/54 (44%), Positives = 32/54 (59%), Gaps = 2/54 (3%)
Frame = +1
Query: 88 ASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR--GTCTPSR 243
AS ++F ++ +L I+D NVMK KGGKMGAVNG G+ +C SR
Sbjct: 724 ASSGLPSLFDDYKIKSSLHKIYDFNVMKVKGGKMGAVNGMHPNGKVDESCMQSR 777
>UniRef50_Q8LPR0 Cluster: AT4g10060/T5L19_190; n=3; Arabidopsis
thaliana|Rep: AT4g10060/T5L19_190 - Arabidopsis thaliana
(Mouse-ear cress)
Length = 922
Score = 46.0 bits (104), Expect = 0.001
Identities = 23/58 (39%), Positives = 35/58 (60%)
Frame = +1
Query: 49 VAIDVFSSTRFLRASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR 222
+ D + + RA G + + + ++KAL+TI++ NVMK KGG GAVNG EG+
Sbjct: 718 ILADQLAGQWYARACG-LKPITKEEWIKKALETIYEFNVMKVKGGTRGAVNGMSTEGK 774
>UniRef50_A3BJA3 Cluster: Putative uncharacterized protein; n=1;
Oryza sativa (japonica cultivar-group)|Rep: Putative
uncharacterized protein - Oryza sativa subsp. japonica
(Rice)
Length = 737
Score = 45.6 bits (103), Expect = 0.001
Identities = 21/49 (42%), Positives = 29/49 (59%)
Frame = +1
Query: 88 ASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGRGTCT 234
AS +F + ++ L+ IFD NVM+ KGG+MGAVNG G+ T
Sbjct: 589 ASSGLPPLFDEGRIKCTLQKIFDYNVMRVKGGRMGAVNGMHPNGKAFTT 637
>UniRef50_A0MNZ0 Cluster: NADPH oxidoreductase; n=1; Bombyx
mori|Rep: NADPH oxidoreductase - Bombyx mori (Silk moth)
Length = 191
Score = 43.2 bits (97), Expect = 0.008
Identities = 16/18 (88%), Positives = 18/18 (100%)
Frame = -1
Query: 612 KWVDDLTAHLVLSGYWSP 559
+WVD+LTAHLVLSGYWSP
Sbjct: 158 RWVDELTAHLVLSGYWSP 175
>UniRef50_Q9LQ24 Cluster: F14M2.16 protein; n=1; Arabidopsis
thaliana|Rep: F14M2.16 protein - Arabidopsis thaliana
(Mouse-ear cress)
Length = 790
Score = 42.7 bits (96), Expect = 0.010
Identities = 20/58 (34%), Positives = 35/58 (60%)
Frame = +1
Query: 49 VAIDVFSSTRFLRASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR 222
+ D + + RA G + + + ++KAL+T++D NVM+ + G GAVNG + +GR
Sbjct: 588 ILADQMAGQWYARACG-LKPIAKEEWIKKALETVYDFNVMRVRDGTRGAVNGMLPDGR 644
>UniRef50_Q9LTY0 Cluster: Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K9P8; n=13; Magnoliophyta|Rep:
Arabidopsis thaliana genomic DNA, chromosome 5, TAC
clone:K9P8 - Arabidopsis thaliana (Mouse-ear cress)
Length = 928
Score = 40.7 bits (91), Expect = 0.042
Identities = 22/58 (37%), Positives = 31/58 (53%)
Frame = +1
Query: 49 VAIDVFSSTRFLRASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR 222
+ D + + RASG V + R AL+ +++ NVMK K GK GAVNG G+
Sbjct: 724 IQADQLAGQWYARASGLLPIV-DEDKARTALEKVYNYNVMKIKDGKRGAVNGMHPNGK 780
>UniRef50_Q0LJ08 Cluster: Putative uncharacterized protein; n=1;
Herpetosiphon aurantiacus ATCC 23779|Rep: Putative
uncharacterized protein - Herpetosiphon aurantiacus ATCC
23779
Length = 774
Score = 39.9 bits (89), Expect = 0.073
Identities = 17/38 (44%), Positives = 26/38 (68%)
Frame = +1
Query: 106 AVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEG 219
AV P+ +R AL+T++ NVM++ G +GAVNG +G
Sbjct: 648 AVAPREMIRSALQTVYRFNVMQYANGALGAVNGMHPDG 685
>UniRef50_A7QC94 Cluster: Chromosome undetermined scaffold_77, whole
genome shotgun sequence; n=1; Vitis vinifera|Rep:
Chromosome undetermined scaffold_77, whole genome
shotgun sequence - Vitis vinifera (Grape)
Length = 925
Score = 39.9 bits (89), Expect = 0.073
Identities = 21/67 (31%), Positives = 35/67 (52%)
Frame = +1
Query: 49 VAIDVFSSTRFLRASGWTEAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEGRGT 228
+ D + + RA G + V + + AL+ ++ NV+K GGK GAVNG + +G+
Sbjct: 728 IQADQLAGQWYARACGLSPIV-DEDKAKSALEKVYHYNVLKVGGGKRGAVNGMLPDGKVD 786
Query: 229 CTPSRCR 249
T + R
Sbjct: 787 TTTMQSR 793
>UniRef50_Q8WQB2 Cluster: Putative uncharacterized protein; n=4;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 959
Score = 39.5 bits (88), Expect = 0.097
Identities = 19/39 (48%), Positives = 23/39 (58%)
Frame = +1
Query: 103 EAVFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEG 219
+ + P VR AL TI+ NV KF G+MGAVNG G
Sbjct: 772 DELLPNHMVRSALDTIYRLNVCKFGNGRMGAVNGMKPSG 810
>UniRef50_Q1IR11 Cluster: Putative uncharacterized protein
precursor; n=1; Acidobacteria bacterium Ellin345|Rep:
Putative uncharacterized protein precursor -
Acidobacteria bacterium (strain Ellin345)
Length = 811
Score = 38.3 bits (85), Expect = 0.22
Identities = 17/35 (48%), Positives = 22/35 (62%)
Frame = +1
Query: 115 PQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEG 219
P+ ALK ++D NVMK + G MGA+NG A G
Sbjct: 692 PRDMQHSALKKVYDFNVMKLQNGTMGALNGISASG 726
>UniRef50_Q7NHS5 Cluster: Gll2460 protein; n=1; Gloeobacter
violaceus|Rep: Gll2460 protein - Gloeobacter violaceus
Length = 867
Score = 36.3 bits (80), Expect = 0.90
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 109 VFPQANVRKALKTIFDNNVMKFKGGKMGAVNGFVAEG 219
+ P+ R AL+T++ +KF GG+ G NG A+G
Sbjct: 736 IVPEPQARSALETVYQTCFVKFYGGRFGCANGTNADG 772
>UniRef50_A0JP97 Cluster: LOC100036642 protein; n=7; Eumetazoa|Rep:
LOC100036642 protein - Xenopus tropicalis (Western
clawed frog) (Silurana tropicalis)
Length = 1110
Score = 34.3 bits (75), Expect = 3.6
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = -3
Query: 139 VLFAHSLAGTPLRSIRTPAGTLWKRTHQWLP*KRNHN 29
V AHS AG TP +WKR +W P +R N
Sbjct: 69 VFLAHSQAGDSSEEAPTPDALVWKRERKWKPSERGFN 105
>UniRef50_Q5LPC3 Cluster: Putative uncharacterized protein; n=1;
Silicibacter pomeroyi|Rep: Putative uncharacterized
protein - Silicibacter pomeroyi
Length = 412
Score = 34.3 bits (75), Expect = 3.6
Identities = 17/36 (47%), Positives = 24/36 (66%)
Frame = +1
Query: 190 GAVNGFVAEGRGTCTPSRCRARGLDRSHVRPGLAHG 297
G V+ +AEGRG TP+RCR RG +R+ + P + G
Sbjct: 189 GLVDVTLAEGRGIGTPARCRRRG-NRARLDPRRSRG 223
>UniRef50_Q52UP0 Cluster: DNA polymerase; n=24; root|Rep: DNA
polymerase - Koi herpesvirus
Length = 1709
Score = 33.9 bits (74), Expect = 4.8
Identities = 19/70 (27%), Positives = 33/70 (47%)
Frame = +2
Query: 482 SRCNYT*DLELIKSQDGWRIYVADVYGLQ*PLNTRWAVRSSTHLSNKKSRILEKFPKIES 661
SRC T L S DG R+Y+ D++ N + ++ ++ K + ++E
Sbjct: 951 SRCPETKHFYLTDSVDGQRVYLKDIFDRTLITNLAYENTATVSINMAKKNYIYTNHELED 1010
Query: 662 VIITKRKRKL 691
++TK K KL
Sbjct: 1011 GVLTKTKEKL 1020
>UniRef50_O01893 Cluster: Putative uncharacterized protein R08F11.1;
n=2; Caenorhabditis|Rep: Putative uncharacterized
protein R08F11.1 - Caenorhabditis elegans
Length = 884
Score = 33.9 bits (74), Expect = 4.8
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = +1
Query: 127 VRKALKTIFDNNVMKFKGGKMGAVNGFVAEGR 222
++ AL TIF NV + G+ GAVNG++ R
Sbjct: 745 MKSALDTIFKYNVQMYNNGRCGAVNGYLTSER 776
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 854,780,137
Number of Sequences: 1657284
Number of extensions: 17643614
Number of successful extensions: 45732
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 43988
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 45717
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 69143070360
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -