Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0167.Seq
(787 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64... 87 1e-17
At2g15230.1 68415.m01737 lipase family protein similar to SP|P07... 76 2e-14
At1g73920.2 68414.m08561 lipase family protein similar to lipase... 60 2e-09
At1g73920.1 68414.m08560 lipase family protein similar to lipase... 60 2e-09
At1g18460.1 68414.m02303 lipase family protein similar to triacy... 56 3e-08
At1g73750.1 68414.m08539 expressed protein contains Pfam profile... 45 7e-05
At1g15060.1 68414.m01800 expressed protein 44 2e-04
At1g51440.1 68414.m05790 lipase class 3 family protein similar t... 34 0.12
At1g24220.1 68414.m03054 paired amphipathic helix repeat-contain... 33 0.21
At2g31490.1 68415.m03846 expressed protein 30 2.0
At1g60360.1 68414.m06796 zinc finger (C3HC4-type RING finger) fa... 29 3.5
At1g61400.1 68414.m06919 S-locus protein kinase, putative simila... 29 4.6
At1g06800.2 68414.m00725 lipase class 3 family protein similar t... 29 4.6
At1g06800.1 68414.m00724 lipase class 3 family protein similar t... 29 4.6
>At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194
Lysosomal acid lipase/cholesteryl ester hydrolase
precursor (EC 3.1.1.13) {Rattus norvegicus}; contains
Pfam profile PF04083: ab-hydrolase associated lipase
region
Length = 418
Score = 87.0 bits (206), Expect = 1e-17
Identities = 39/85 (45%), Positives = 56/85 (65%), Gaps = 5/85 (5%)
Frame = +2
Query: 269 GYRCETHSLISQ-GYVLNIHRIPQARSG---GDTPSNT-VILQHGLFASSADWVLNGPGK 433
GY+CE H +++Q GY+LN+ RIP+ R+G GD V++QHG+ W+LN +
Sbjct: 60 GYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGMSWLLNPADQ 119
Query: 434 SLAFVLADAGYDVWMPNIRGNRYSR 508
+L +LAD G+DVWM N RG R+SR
Sbjct: 120 NLPLILADQGFDVWMGNTRGTRFSR 144
Score = 63.7 bits (148), Expect = 1e-10
Identities = 31/82 (37%), Positives = 48/82 (58%)
Frame = +1
Query: 511 HTTLKSSSTQYWNFSWHEVAQHDIPAIIDYIRERKGSDTKIAYMGHSMGSTMLFAMLALR 690
H L S +WN++W E+ +D+PA+ D+I G KI Y+GHS+G+ + FA + +
Sbjct: 146 HKYLNPSQRAFWNWTWDELVSYDLPAMFDHIHGLTGQ--KIHYLGHSLGTLIGFASFSEK 203
Query: 691 PEYNAVLRAGLALGPVVYLSHI 756
+ V R+ L PV YLSH+
Sbjct: 204 GLVDQV-RSAAMLSPVAYLSHM 224
>At2g15230.1 68415.m01737 lipase family protein similar to SP|P07098
Triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Homo sapiens}; contains Pfam profile PF04083:
ab-hydrolase associated lipase region
Length = 393
Score = 76.2 bits (179), Expect = 2e-14
Identities = 40/88 (45%), Positives = 54/88 (61%), Gaps = 2/88 (2%)
Frame = +2
Query: 248 ARVITRRGYRCETHSLISQ-GYVLNIHRIPQARSGGDTPSNT-VILQHGLFASSADWVLN 421
A +I Y C HS+ ++ GY+L + R+ A G S V+LQHGLF + W LN
Sbjct: 34 ADLIHPANYSCTEHSIQTKDGYILALQRV--ASLGPRLQSGPPVLLQHGLFMAGDVWFLN 91
Query: 422 GPGKSLAFVLADAGYDVWMPNIRGNRYS 505
P +SL F+LAD G+DVW+ N+RG RYS
Sbjct: 92 SPKESLGFILADHGFDVWVGNVRGTRYS 119
Score = 61.3 bits (142), Expect = 7e-10
Identities = 28/82 (34%), Positives = 48/82 (58%)
Frame = +1
Query: 511 HTTLKSSSTQYWNFSWHEVAQHDIPAIIDYIRERKGSDTKIAYMGHSMGSTMLFAMLALR 690
H TL + ++W++SW ++A +D+ +I Y+ S++KI +GHS G+ M FA L +
Sbjct: 122 HVTLSDTDKEFWDWSWQDLAMYDLAEMIQYLYSI--SNSKIFLVGHSQGTIMSFAALT-Q 178
Query: 691 PEYNAVLRAGLALGPVVYLSHI 756
P ++ A L P+ YL H+
Sbjct: 179 PHVAEMVEAAALLCPISYLDHV 200
>At1g73920.2 68414.m08561 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 635
Score = 59.7 bits (138), Expect = 2e-09
Identities = 37/81 (45%), Positives = 44/81 (54%), Gaps = 1/81 (1%)
Frame = +2
Query: 254 VITRRGYRCETHSLI-SQGYVLNIHRIPQARSGGDTPSNTVILQHGLFASSADWVLNGPG 430
VIT GY E +I S GYVL + RIP+ + V LQHG+ SS WV NG
Sbjct: 230 VITELGYPYEAIRVITSDGYVLVLERIPRRDA-----RKAVFLQHGVLDSSMGWVSNGVV 284
Query: 431 KSLAFVLADAGYDVWMPNIRG 493
S AF D GYDV++ N RG
Sbjct: 285 GSPAFAAYDQGYDVFLGNFRG 305
Score = 36.3 bits (80), Expect = 0.023
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +1
Query: 499 IFEEHTTLKSSSTQYWNFSWHEVAQHDIPAIIDYIRERKGSDTKI 633
+ +H SS ++W +S +E DIPA+I+ I E K ++ K+
Sbjct: 307 VSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEKIHEIKTTELKL 351
>At1g73920.1 68414.m08560 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 704
Score = 59.7 bits (138), Expect = 2e-09
Identities = 37/81 (45%), Positives = 44/81 (54%), Gaps = 1/81 (1%)
Frame = +2
Query: 254 VITRRGYRCETHSLI-SQGYVLNIHRIPQARSGGDTPSNTVILQHGLFASSADWVLNGPG 430
VIT GY E +I S GYVL + RIP+ + V LQHG+ SS WV NG
Sbjct: 299 VITELGYPYEAIRVITSDGYVLVLERIPRRDA-----RKAVFLQHGVLDSSMGWVSNGVV 353
Query: 431 KSLAFVLADAGYDVWMPNIRG 493
S AF D GYDV++ N RG
Sbjct: 354 GSPAFAAYDQGYDVFLGNFRG 374
Score = 36.3 bits (80), Expect = 0.023
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +1
Query: 499 IFEEHTTLKSSSTQYWNFSWHEVAQHDIPAIIDYIRERKGSDTKI 633
+ +H SS ++W +S +E DIPA+I+ I E K ++ K+
Sbjct: 376 VSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEKIHEIKTTELKL 420
>At1g18460.1 68414.m02303 lipase family protein similar to
triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Canis familiaris} [SP|P80035]
Length = 701
Score = 56.0 bits (129), Expect = 3e-08
Identities = 35/81 (43%), Positives = 43/81 (53%), Gaps = 1/81 (1%)
Frame = +2
Query: 254 VITRRGYRCETHSLI-SQGYVLNIHRIPQARSGGDTPSNTVILQHGLFASSADWVLNGPG 430
VIT GY E ++ S GY L + RIP+ + V LQHG+ SS WV NG
Sbjct: 297 VITELGYPYEAIRVVTSDGYGLLLERIPRRDA-----RKAVYLQHGVMDSSMGWVSNGVV 351
Query: 431 KSLAFVLADAGYDVWMPNIRG 493
S AF D GYDV++ N RG
Sbjct: 352 GSPAFAAYDQGYDVFLGNFRG 372
Score = 37.9 bits (84), Expect = 0.008
Identities = 17/45 (37%), Positives = 26/45 (57%)
Frame = +1
Query: 499 IFEEHTTLKSSSTQYWNFSWHEVAQHDIPAIIDYIRERKGSDTKI 633
+ +H SS +W +S +E A DIPA+I+ I E K S+ K+
Sbjct: 374 VSRDHVKKNISSKDFWRYSINEHATEDIPAMIEKIHEIKTSELKL 418
>At1g73750.1 68414.m08539 expressed protein contains Pfam profile:
PF00561 alpha/beta hydrolase fold
Length = 452
Score = 44.8 bits (101), Expect = 7e-05
Identities = 17/49 (34%), Positives = 33/49 (67%), Gaps = 1/49 (2%)
Frame = +1
Query: 541 YWNFSWHEVAQHDIPAIIDYIR-ERKGSDTKIAYMGHSMGSTMLFAMLA 684
++N+ + + D+P+ +DY+R + K D K+ +GHSMG +L+A+L+
Sbjct: 200 HYNWDFDNYLEEDVPSAMDYVRTQTKSKDGKLLAVGHSMGGILLYALLS 248
>At1g15060.1 68414.m01800 expressed protein
Length = 578
Score = 43.6 bits (98), Expect = 2e-04
Identities = 20/48 (41%), Positives = 32/48 (66%), Gaps = 1/48 (2%)
Frame = +1
Query: 544 WNFSWHEVAQHDIPAIIDYIR-ERKGSDTKIAYMGHSMGSTMLFAMLA 684
W+F + + D+PA I+Y+R + K D K+ +GHSMG +L+AML+
Sbjct: 329 WDFDHY--LEEDVPAAIEYVRAQSKPKDGKLFAIGHSMGGILLYAMLS 374
>At1g51440.1 68414.m05790 lipase class 3 family protein similar to
DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana]
GI:16215706, lipase [Dianthus caryophyllus] GI:4103627;
contains Pfam profile PF01764: Lipase
Length = 527
Score = 33.9 bits (74), Expect = 0.12
Identities = 17/56 (30%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = +1
Query: 502 FEEHTTLKSSSTQYWNFSWHEVAQHDIPAIIDYI-RERKGSDTKIAYMGHSMGSTM 666
F + T K S ++ +FS E ++ +I+Y E +G T I GHS+G+++
Sbjct: 252 FHDLYTKKEDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASL 307
>At1g24220.1 68414.m03054 paired amphipathic helix repeat-containing
protein weak similarity to transcription co-repressor
Sin3 [Xenopus laevis] GI:4960210; contains Pfam profile
PF02671: Paired amphipathic helix repeat
Length = 744
Score = 33.1 bits (72), Expect = 0.21
Identities = 15/43 (34%), Positives = 26/43 (60%)
Frame = +3
Query: 57 LFGSSRSLVNNFEDARNYIETQKDKVIEEWSSYMEDIKLSSSW 185
+FG S L +DAR+YI++ K+ +E + Y E +KL + +
Sbjct: 163 MFGKSVPLEKTLDDARSYIDSVKEAFHDEPAKYAEFLKLLNDY 205
>At2g31490.1 68415.m03846 expressed protein
Length = 71
Score = 29.9 bits (64), Expect = 2.0
Identities = 11/26 (42%), Positives = 18/26 (69%)
Frame = +3
Query: 111 IETQKDKVIEEWSSYMEDIKLSSSWT 188
+ET K+K IE+W S E+++ + WT
Sbjct: 5 METNKNKFIEDWGSARENLEHNFRWT 30
>At1g60360.1 68414.m06796 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 327
Score = 29.1 bits (62), Expect = 3.5
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = -2
Query: 735 GAEREPRPQHRVVLRPQRQHREQHRGTHRVAHVRDLCVRALPLPDIVY 592
GA+ R + R +L P+ + R QHR H + +V + LPLP Y
Sbjct: 98 GADPFLRARSRNILEPESRPRPQHRRRHSLDNVNN---GGLPLPRRTY 142
>At1g61400.1 68414.m06919 S-locus protein kinase, putative similar
to KI domain interacting kinase 1 [Zea mays]
gi|2735017|gb|AAB93834; contains S-locus glycoprotein
family domain, Pfam:PF00954
Length = 821
Score = 28.7 bits (61), Expect = 4.6
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 337 GSKRRRHSIEYRDTSARLVCELCRLGPERAW 429
G R H EY+D + R+V L + PE AW
Sbjct: 652 GLARMFHGTEYQDKTRRVVGTLGYMSPEYAW 682
>At1g06800.2 68414.m00725 lipase class 3 family protein similar to
DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana]
GI:16215706; contains Pfam profile PF01764: Lipase
Length = 444
Score = 28.7 bits (61), Expect = 4.6
Identities = 12/50 (24%), Positives = 24/50 (48%)
Frame = +1
Query: 517 TLKSSSTQYWNFSWHEVAQHDIPAIIDYIRERKGSDTKIAYMGHSMGSTM 666
T K +S + FS E ++ +++ + +G + I GHS+G +
Sbjct: 259 TDKDTSCNFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGAL 308
>At1g06800.1 68414.m00724 lipase class 3 family protein similar to
DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana]
GI:16215706; contains Pfam profile PF01764: Lipase
Length = 515
Score = 28.7 bits (61), Expect = 4.6
Identities = 12/50 (24%), Positives = 24/50 (48%)
Frame = +1
Query: 517 TLKSSSTQYWNFSWHEVAQHDIPAIIDYIRERKGSDTKIAYMGHSMGSTM 666
T K +S + FS E ++ +++ + +G + I GHS+G +
Sbjct: 259 TDKDTSCNFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGAL 308
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,552,684
Number of Sequences: 28952
Number of extensions: 313005
Number of successful extensions: 960
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 911
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 953
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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