Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0165.Seq
(764 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 71 1e-12
At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 71 1e-12
At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 68 6e-12
At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 66 2e-11
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 57 1e-08
At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 56 3e-08
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 55 5e-08
At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 53 2e-07
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 38 0.006
At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 37 0.017
At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 32 0.48
At2g28880.1 68415.m03510 para-aminobenzoate (PABA) synthase fami... 32 0.48
At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) fa... 30 1.5
At2g15090.1 68415.m01720 fatty acid elongase, putative similar ... 30 1.5
At4g21900.1 68417.m03166 MATE efflux family protein similar to r... 29 2.6
At4g34250.1 68417.m04868 fatty acid elongase, putative similar t... 29 3.4
At1g04980.1 68414.m00497 thioredoxin family protein similar to S... 29 3.4
>At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5
[Arabidopsis thaliana] GI:4240122; strong similarity to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 140
Score = 70.5 bits (165), Expect = 1e-12
Identities = 32/73 (43%), Positives = 47/73 (64%)
Frame = +1
Query: 19 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 198
+T +EV+ + K I+ID VYDV FL+DHPGG EV++ + G DA++ F +VGHS
Sbjct: 8 FTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSST 67
Query: 199 AIEWRNTFKVGEI 237
A + + VG+I
Sbjct: 68 AKAMLDEYYVGDI 80
>At2g32720.1 68415.m04004 cytochrome b5, putative similar to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 134
Score = 70.5 bits (165), Expect = 1e-12
Identities = 33/73 (45%), Positives = 47/73 (64%)
Frame = +1
Query: 19 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 198
+T +EV+ + I+I+ VY+V FLEDHPGG +VL+ + G DA++ F +VGHSE
Sbjct: 8 FTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSES 67
Query: 199 AIEWRNTFKVGEI 237
A E + VGEI
Sbjct: 68 AREMMEQYYVGEI 80
>At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to
SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana]
Length = 134
Score = 68.1 bits (159), Expect = 6e-12
Identities = 30/69 (43%), Positives = 44/69 (63%)
Frame = +1
Query: 31 EVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEIAIEW 210
EV+ + K +II VYDV F++DHPGG EVL+ + G DA+ F +VGHS+ A +
Sbjct: 12 EVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTARDM 71
Query: 211 RNTFKVGEI 237
+ + +GEI
Sbjct: 72 MDKYFIGEI 80
>At1g26340.1 68414.m03212 cytochrome b5, putative similar to
cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis
thaliana]
Length = 135
Score = 66.1 bits (154), Expect = 2e-11
Identities = 30/79 (37%), Positives = 49/79 (62%)
Frame = +1
Query: 19 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 198
Y+ E A + ++ID VYDV++++++HPGG +VL+ AG DA++ F + GHS+
Sbjct: 8 YSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKD 67
Query: 199 AIEWRNTFKVGEIVDEEKL 255
A E + +GE+ DE L
Sbjct: 68 ARELMEKYFIGEL-DESSL 85
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 56.8 bits (131), Expect = 1e-08
Identities = 26/74 (35%), Positives = 45/74 (60%)
Frame = +1
Query: 19 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 198
Y+ +EV + II+ +YD FL+DHPGGT+ ++ NAG+D +E F + HS+
Sbjct: 548 YSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAI-HSDK 606
Query: 199 AIEWRNTFKVGEIV 240
A + +++GE++
Sbjct: 607 AKKLLEDYRIGELI 620
>At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein
contains InterPro accession IPR001199: Cytochrome b5
Length = 121
Score = 55.6 bits (128), Expect = 3e-08
Identities = 24/73 (32%), Positives = 45/73 (61%)
Frame = +1
Query: 19 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 198
Y+++EVA + III + VYD+ +++E+HPGG + ++D+AG D+++ F H+
Sbjct: 49 YSKSEVAVHNKRNDCWIIIKDKVYDITSYVEEHPGG-DAILDHAGDDSTDGFFGPQHATR 107
Query: 199 AIEWRNTFKVGEI 237
+ F +GE+
Sbjct: 108 VFDMIEDFYIGEL 120
>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
(NR2) {Arabidopsis thaliana}
Length = 917
Score = 55.2 bits (127), Expect = 5e-08
Identities = 25/74 (33%), Positives = 45/74 (60%)
Frame = +1
Query: 19 YTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEI 198
Y+ +EV ++ II+ +YD FL DHPGG++ ++ NAG+D +E F + HS+
Sbjct: 545 YSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAI-HSDK 603
Query: 199 AIEWRNTFKVGEIV 240
A + +++GE++
Sbjct: 604 AKKMLEDYRIGELI 617
>At2g46650.1 68415.m05820 cytochrome b5, putative similar to
cytochome b5 GI:2695711 from [Olea europaea]
Length = 132
Score = 53.2 bits (122), Expect = 2e-07
Identities = 23/57 (40%), Positives = 36/57 (63%)
Frame = +1
Query: 67 IIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEIAIEWRNTFKVGEI 237
I+I VYD++ F+++HPGG VL+ G DAS F +V HS+ A E + +G++
Sbjct: 21 ILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAKELMKKYCIGDV 77
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 38.3 bits (85), Expect = 0.006
Identities = 18/54 (33%), Positives = 31/54 (57%), Gaps = 1/54 (1%)
Frame = +1
Query: 16 KYTRAEVAARDNNKQNL-IIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECF 174
+Y +E + N +L I I VYDV+ +++ HPGG +++ AG D ++ F
Sbjct: 8 RYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61
>At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1)
identical to delta-8 sphingolipid desaturase GI:3819710
from [Arabidopsis thaliana]; contains Pfam profile
PF00487: Fatty acid desaturase; contains Pfam profile
PF00173: Heme/Steroid binding domain
Length = 449
Score = 36.7 bits (81), Expect = 0.017
Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +1
Query: 16 KYTRAEVAARDNNKQNL-IIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECF 174
KY E + N +L I I VY+V+ +++ HPGG V+++ G D ++ F
Sbjct: 8 KYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61
>At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein
flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803
Length = 211
Score = 31.9 bits (69), Expect = 0.48
Identities = 14/63 (22%), Positives = 36/63 (57%)
Frame = +1
Query: 70 IIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHEVGHSEIAIEWRNTFKVGEIVDEE 249
++ VY+++ ++ HPGG ++L+ G D + F++ H+ + ++ + ++D+
Sbjct: 146 VLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKY-HAWVNVDILLEKCLVGVLDDT 204
Query: 250 KLK 258
K+K
Sbjct: 205 KVK 207
>At2g28880.1 68415.m03510 para-aminobenzoate (PABA) synthase family
protein similar to PABA synthase from Streptomyces
griseus [SP|P32483], Streptomyces pristinaespiralis
[gi:1575336]; contains Pfam profiles PF00425:
chorismate binding enzyme, PF00117: glutamine
amidotransferase class-I, PF04715: Anthranilate synthase
component I, N terminal region
Length = 919
Score = 31.9 bits (69), Expect = 0.48
Identities = 20/45 (44%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = -1
Query: 263 FHFNFSSSTIS-PTLNVLRHSMAISLWPTSWKHSEASEPALSTRT 132
F F +SS +S P+ NVLR S+A L+ WK S S P S T
Sbjct: 5 FSFCSTSSELSYPSENVLRFSVASRLFSPKWKKSFISLPCRSKTT 49
>At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) family
protein contains Pfam profile: PF00097 zinc finger, C3HC4
type (RING finger)
Length = 4706
Score = 30.3 bits (65), Expect = 1.5
Identities = 16/36 (44%), Positives = 23/36 (63%), Gaps = 2/36 (5%)
Frame = -1
Query: 212 RHSMAISL--WPTSWKHSEASEPALSTRTSVPPGWS 111
R+S A+ L WP +W +S P LST+ +PPG+S
Sbjct: 4015 RYSEAVLLRDWPLAW----SSVPILSTQRFIPPGFS 4046
>At2g15090.1 68415.m01720 fatty acid elongase, putative similar to
fatty acid elongase 1 [GI:881615]
Length = 481
Score = 30.3 bits (65), Expect = 1.5
Identities = 20/64 (31%), Positives = 31/64 (48%)
Frame = +1
Query: 1 IMSVHKYTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHE 180
++ VH+ T A V + +N QNL + N V L GG VL+ N D + +E
Sbjct: 226 LLQVHRNTYAIVVSTENITQNLYLGKNKSMLVTNCL-FRVGGAAVLLSNRSRDRNRAKYE 284
Query: 181 VGHS 192
+ H+
Sbjct: 285 LVHT 288
>At4g21900.1 68417.m03166 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
MatE
Length = 1094
Score = 29.5 bits (63), Expect = 2.6
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 491 KWVIKKSGWAIVVQCLSCDVRKPCL 565
KW++KK + +CLSCD C+
Sbjct: 726 KWIVKKGNVSSAGKCLSCDEHLACV 750
>At4g34250.1 68417.m04868 fatty acid elongase, putative similar to
fatty acid elongase 1 (Fae1), Arabidopsis thaliana,
U29142 [GI:881615]
Length = 493
Score = 29.1 bits (62), Expect = 3.4
Identities = 19/64 (29%), Positives = 31/64 (48%)
Frame = +1
Query: 1 IMSVHKYTRAEVAARDNNKQNLIIIDNVVYDVAAFLEDHPGGTEVLVDNAGSDASECFHE 180
++ VH+ T A V + +N QNL + +N V L GG +L+ N D +E
Sbjct: 234 LLQVHRNTYALVVSTENITQNLYMGNNKSMLVTNCL-FRIGGAAILLSNRSIDRKRAKYE 292
Query: 181 VGHS 192
+ H+
Sbjct: 293 LVHT 296
>At1g04980.1 68414.m00497 thioredoxin family protein similar to
SP|Q63081 Protein disulfide isomerase A6 precursor (EC
5.3.4.1) {Rattus norvegicus}; contains Pfam profile
PF00085: Thioredoxin
Length = 443
Score = 29.1 bits (62), Expect = 3.4
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +2
Query: 395 PVQCALFAKTTSGHLQHASSSDIRMDLT*SGFKWVIKKSGWAIVVQ 532
P+ C LFA G+ + SSS + + LT S FK + S ++V+
Sbjct: 8 PICCLLFALFDRGNALYGSSSPV-LQLTPSNFKSKVLNSNGVVLVE 52
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,792,913
Number of Sequences: 28952
Number of extensions: 259572
Number of successful extensions: 631
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 618
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 630
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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