Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0163.Seq
(720 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 173 2e-44
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 104 1e-23
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 100 2e-22
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 99 6e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 72 1e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 67 3e-12
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 58 1e-09
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 46 5e-06
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 44 2e-05
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.17
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.29
SPAC2E1P3.04 |||copper amine oxidase |Schizosaccharomyces pombe|... 28 1.2
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 28 1.2
SPBC14C8.07c |cdc18||MCM loader|Schizosaccharomyces pombe|chr 2|... 27 2.0
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.7
SPCP1E11.10 |||ankyrin repeat protein, unknown biological role|S... 26 4.7
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.7
SPCC306.02c |||Rab GTPase binding |Schizosaccharomyces pombe|chr... 26 6.2
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 25 8.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 173 bits (422), Expect = 2e-44
Identities = 78/89 (87%), Positives = 83/89 (93%)
Frame = +1
Query: 256 ITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVA 435
+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETFN+PA YVA
Sbjct: 76 VNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVA 135
Query: 436 IQAVLSLYASGRTTGIVLDSGDGVSTPYP 522
IQAVLSLYASGRTTGIVLDSGDGV+ P
Sbjct: 136 IQAVLSLYASGRTTGIVLDSGDGVTHTVP 164
Score = 150 bits (364), Expect = 2e-37
Identities = 66/73 (90%), Positives = 72/73 (98%)
Frame = +2
Query: 35 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 214
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 215 RGILTLKYPIEHG 253
RGILTLKYPIEHG
Sbjct: 62 RGILTLKYPIEHG 74
Score = 122 bits (295), Expect = 4e-29
Identities = 56/60 (93%), Positives = 59/60 (98%)
Frame = +3
Query: 510 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVA 689
HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVA
Sbjct: 161 HTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVA 220
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 104 bits (249), Expect = 1e-23
Identities = 45/89 (50%), Positives = 66/89 (74%)
Frame = +1
Query: 256 ITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVA 435
I ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE + +YVA
Sbjct: 76 IRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVA 135
Query: 436 IQAVLSLYASGRTTGIVLDSGDGVSTPYP 522
IQAVLSLYA G ++G+V+DSGDGV+ P
Sbjct: 136 IQAVLSLYAQGLSSGVVVDSGDGVTHIVP 164
Score = 77.0 bits (181), Expect = 3e-15
Identities = 33/60 (55%), Positives = 44/60 (73%)
Frame = +3
Query: 510 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVA 689
H VP+YE L H + RLD+AGRD T YL+ +L +GY+F TA+ E VR++KEKLCYV+
Sbjct: 161 HIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVS 220
Score = 69.7 bits (163), Expect = 4e-13
Identities = 35/71 (49%), Positives = 48/71 (67%), Gaps = 2/71 (2%)
Frame = +2
Query: 47 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 220
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 221 ILTLKYPIEHG 253
+L +KYP+E+G
Sbjct: 64 LLQVKYPMENG 74
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 100 bits (240), Expect = 2e-22
Identities = 46/90 (51%), Positives = 63/90 (70%), Gaps = 1/90 (1%)
Frame = +1
Query: 256 ITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYV 432
I NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET N PA+
Sbjct: 80 IQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSF 139
Query: 433 AIQAVLSLYASGRTTGIVLDSGDGVSTPYP 522
++Q VL+LYAS RTTGIVL+ GDG++ P
Sbjct: 140 SLQPVLALYASARTTGIVLECGDGLTHSVP 169
Score = 85.0 bits (201), Expect = 1e-17
Identities = 38/71 (53%), Positives = 50/71 (70%)
Frame = +2
Query: 41 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 220
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 221 ILTLKYPIEHG 253
+L ++ PIE G
Sbjct: 68 LLKIQRPIERG 78
Score = 78.2 bits (184), Expect = 1e-15
Identities = 33/61 (54%), Positives = 48/61 (78%)
Frame = +3
Query: 510 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVA 689
H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EIVR+IKEK CYVA
Sbjct: 166 HSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVA 225
Query: 690 S 692
S
Sbjct: 226 S 226
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 99.1 bits (236), Expect = 6e-22
Identities = 50/105 (47%), Positives = 64/105 (60%), Gaps = 8/105 (7%)
Frame = +1
Query: 232 EVPHRARYITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETF 411
+ P R I NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+F
Sbjct: 91 DYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESF 150
Query: 412 NSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSTPYP 522
N +Y+A+QAVL+L AS + TG V+DSGDGV+ P
Sbjct: 151 NCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIP 195
Score = 44.8 bits (101), Expect = 1e-05
Identities = 25/61 (40%), Positives = 36/61 (59%), Gaps = 2/61 (3%)
Frame = +3
Query: 510 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCY 683
H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER IKE+ CY
Sbjct: 192 HIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCY 246
Query: 684 V 686
V
Sbjct: 247 V 247
Score = 42.7 bits (96), Expect = 5e-05
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +2
Query: 53 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 202
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ +
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 71.7 bits (168), Expect = 1e-13
Identities = 34/94 (36%), Positives = 52/94 (55%), Gaps = 2/94 (2%)
Frame = +1
Query: 223 PHSEVPHRAR--YITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 396
P E+ + R ++ NWD +W + +L+ P EHP+L+TE NP NR K +
Sbjct: 61 PGMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120
Query: 397 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 498
MFE+ PA Y+A Q + +ASG+ T ++D G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIG 154
Score = 40.7 bits (91), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +2
Query: 38 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 139
D+V+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 66.9 bits (156), Expect = 3e-12
Identities = 34/97 (35%), Positives = 56/97 (57%), Gaps = 2/97 (2%)
Frame = +1
Query: 238 PHRARYITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNS 417
P I+NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N+
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 418 PAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSTPYP 522
P++ I + S Y + + ++GIVL+ G+ S P
Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIP 181
Score = 36.7 bits (81), Expect = 0.003
Identities = 17/42 (40%), Positives = 27/42 (64%)
Frame = +2
Query: 23 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 148
F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 58.4 bits (135), Expect = 1e-09
Identities = 25/89 (28%), Positives = 50/89 (56%)
Frame = +1
Query: 256 ITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVA 435
I +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Y+
Sbjct: 77 IQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLT 136
Query: 436 IQAVLSLYASGRTTGIVLDSGDGVSTPYP 522
QAV +A+ ++T +++D G ++ P
Sbjct: 137 KQAVCVAFANSKSTALIVDIGSDNASVTP 165
Score = 36.3 bits (80), Expect = 0.004
Identities = 18/57 (31%), Positives = 33/57 (57%)
Frame = +2
Query: 38 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 208
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 46.0 bits (104), Expect = 5e-06
Identities = 21/60 (35%), Positives = 37/60 (61%)
Frame = +3
Query: 510 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVA 689
H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV+
Sbjct: 169 HIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKESVCYVS 226
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 44.0 bits (99), Expect = 2e-05
Identities = 27/96 (28%), Positives = 44/96 (45%), Gaps = 4/96 (4%)
Frame = +1
Query: 223 PHSEVPHRARYITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMT 390
P+ P + + +W+ ++ W H Y+ L P + +PV L +RE T
Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166
Query: 391 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 498
Q FE P +A + ++ LYA G G+V+D G
Sbjct: 167 QFFFEECQVPGFTIAYEPLMGLYAIGILHGLVIDIG 202
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.17
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +2
Query: 98 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 241
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.29
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +1
Query: 298 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 441
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC2E1P3.04 |||copper amine oxidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 712
Score = 28.3 bits (60), Expect = 1.2
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +3
Query: 612 ERGYSFTTTAEREIVRDIKEKLCYVASTSSRRW 710
E Y T ER + + +KE +C S +SR W
Sbjct: 493 ENYYGNAFTTERTVPKTVKEAICDYNSDTSRTW 525
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 28.3 bits (60), Expect = 1.2
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +1
Query: 463 SGRTTGIVLDSGDGVSTPYPSTKVTLCPTPSSVWTWLV 576
+GR G++ +G G ST + L PTPSS+ +L+
Sbjct: 300 NGRRYGLIAPNGSGKSTLLHAIACGLIPTPSSLDFYLL 337
>SPBC14C8.07c |cdc18||MCM loader|Schizosaccharomyces pombe|chr
2|||Manual
Length = 577
Score = 27.5 bits (58), Expect = 2.0
Identities = 18/51 (35%), Positives = 25/51 (49%)
Frame = +3
Query: 561 LDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVASTSSRRWP 713
LD+ R L K +T + SFT +EI IK +L A+TS + P
Sbjct: 322 LDMTDRFLPRLRTKHITPKLLSFTPYTAQEISTIIKARLKTAATTSEKNNP 372
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 27.1 bits (57), Expect = 2.7
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +1
Query: 505 VSTPYPSTKVTLCPTPSSVWTW 570
V++P STK CP PS W W
Sbjct: 47 VASPV-STKTATCPDPSWEWAW 67
>SPCP1E11.10 |||ankyrin repeat protein, unknown biological
role|Schizosaccharomyces pombe|chr 3|||Manual
Length = 207
Score = 26.2 bits (55), Expect = 4.7
Identities = 14/56 (25%), Positives = 31/56 (55%)
Frame = +1
Query: 226 HSEVPHRARYITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 393
+ E P A+Y+ ++ D+E +T N+ ++ E+ L+TE ++ +A + + Q
Sbjct: 111 NGEFPELAKYLYSFTDLEPKDVNTLPNDTKI---EYAKLMTEQEMDEEAGQPLLDQ 163
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 4.7
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 340 LLTEAPLNPKANREKMTQIMFETFN 414
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC306.02c |||Rab GTPase binding |Schizosaccharomyces pombe|chr
3|||Manual
Length = 171
Score = 25.8 bits (54), Expect = 6.2
Identities = 17/57 (29%), Positives = 28/57 (49%)
Frame = +1
Query: 364 PKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSTPYPSTKV 534
P+ E ++I F + Y+AI A+L +YA R +++ G GV Y K+
Sbjct: 44 PRNFSEAQSRISFNFSRFSSNYLAIIAMLVIYALIRNPLLLIVIGIGVGGVYGIRKL 100
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.4 bits (53), Expect = 8.2
Identities = 9/36 (25%), Positives = 21/36 (58%)
Frame = +3
Query: 522 IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 629
+ EG +P++ ++++ G D+T MK+L + +
Sbjct: 308 VEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,801,209
Number of Sequences: 5004
Number of extensions: 56902
Number of successful extensions: 237
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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