Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0157.Seq
         (727 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22...    66   5e-12
SPAC926.06c |||leucine-rich repeat protein, unknown|Schizosaccha...    38   0.001
SPBC1861.08c |||U2 snRNP-associated protein Lea1 |Schizosaccharo...    32   0.073
SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz...    30   0.29 
SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo...    28   1.2  
SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces...    27   2.7  
SPBC800.06 |brx1||ribosome biogenesis protein Brx1|Schizosacchar...    26   4.8  
SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4 |Schi...    26   4.8  
SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces...    26   4.8  
SPAC11E3.14 |||conserved protein|Schizosaccharomyces pombe|chr 1...    26   6.3  
SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|ch...    26   6.3  
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo...    25   8.3  

>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 332

 Score = 66.1 bits (154), Expect = 5e-12
 Identities = 34/89 (38%), Positives = 52/89 (58%)
 Frame = +1

Query: 256 NLEILVLQANRLTKIENLQQLNKLDQLYISENGITVIENLENQIKLQTLDLAVNRITVIE 435
           NL  L L  N +  I N+  L  L+ L+  +N I  IENLE   +L  L+L  N+I VIE
Sbjct: 105 NLTYLDLSFNNIKTIRNINHLKGLENLFFVQNRIRRIENLEGLDRLTNLELGGNKIRVIE 164

Query: 436 NVRHMSDLEELWLNDNQITEWSSIDICNK 522
           N+  + +LE+LW+  N+IT++ + +   K
Sbjct: 165 NLDTLVNLEKLWVGKNKITKFENFEKLQK 193



 Score = 65.7 bits (153), Expect = 6e-12
 Identities = 36/90 (40%), Positives = 53/90 (58%), Gaps = 1/90 (1%)
 Frame = +1

Query: 247 GFDNLEILVLQANRLTKIENLQQLNKLDQLYISENGITVIENLENQIKLQTLDLAVNRIT 426
           G D L  L L  N++  IENL  L  L++L++ +N IT  EN E   KL  L +  NRIT
Sbjct: 146 GLDRLTNLELGGNKIRVIENLDTLVNLEKLWVGKNKITKFENFEKLQKLSLLSIQSNRIT 205

Query: 427 VIENVRHMSD-LEELWLNDNQITEWSSIDI 513
             EN+  +S  L EL+++ N +T +S I++
Sbjct: 206 QFENLACLSHCLRELYVSHNGLTSFSGIEV 235



 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 34/94 (36%), Positives = 55/94 (58%)
 Frame = +1

Query: 250 FDNLEILVLQANRLTKIENLQQLNKLDQLYISENGITVIENLENQIKLQTLDLAVNRITV 429
           F NL+ L L+ N++ KIE++ +   L +L + +N I  IENL+N   L  LDL+ N I  
Sbjct: 61  FKNLQSLCLRQNQIKKIESVPET--LTELDLYDNLIVRIENLDNVKNLTYLDLSFNNIKT 118

Query: 430 IENVRHMSDLEELWLNDNQITEWSSIDICNKTTN 531
           I N+ H+  LE L+   N+I    +++  ++ TN
Sbjct: 119 IRNINHLKGLENLFFVQNRIRRIENLEGLDRLTN 152



 Score = 60.1 bits (139), Expect = 3e-10
 Identities = 31/83 (37%), Positives = 49/83 (59%)
 Frame = +1

Query: 259 LEILVLQANRLTKIENLQQLNKLDQLYISENGITVIENLENQIKLQTLDLAVNRITVIEN 438
           LE L    NR+ +IENL+ L++L  L +  N I VIENL+  + L+ L +  N+IT  EN
Sbjct: 128 LENLFFVQNRIRRIENLEGLDRLTNLELGGNKIRVIENLDTLVNLEKLWVGKNKITKFEN 187

Query: 439 VRHMSDLEELWLNDNQITEWSSI 507
              +  L  L +  N+IT++ ++
Sbjct: 188 FEKLQKLSLLSIQSNRITQFENL 210



 Score = 59.7 bits (138), Expect = 4e-10
 Identities = 29/85 (34%), Positives = 53/85 (62%), Gaps = 1/85 (1%)
 Frame = +1

Query: 259 LEILVLQANRLTKIENLQQLNK-LDQLYISENGITVIENLENQIKLQTLDLAVNRITVIE 435
           L +L +Q+NR+T+ ENL  L+  L +LY+S NG+T    +E    L+ LD++ N I  + 
Sbjct: 194 LSLLSIQSNRITQFENLACLSHCLRELYVSHNGLTSFSGIEVLENLEILDVSNNMIKHLS 253

Query: 436 NVRHMSDLEELWLNDNQITEWSSID 510
            +  + +L ELW ++N+++ +  I+
Sbjct: 254 YLAGLKNLVELWASNNELSSFQEIE 278



 Score = 46.0 bits (104), Expect = 6e-06
 Identities = 29/86 (33%), Positives = 42/86 (48%)
 Frame = +1

Query: 253 DNLEILVLQANRLTKIENLQQLNKLDQLYISENGITVIENLENQIKLQTLDLAVNRITVI 432
           + L  L L  N + +IENL  +  L  L +S N I  I N+ +   L+ L    NRI  I
Sbjct: 82  ETLTELDLYDNLIVRIENLDNVKNLTYLDLSFNNIKTIRNINHLKGLENLFFVQNRIRRI 141

Query: 433 ENVRHMSDLEELWLNDNQITEWSSID 510
           EN+  +  L  L L  N+I    ++D
Sbjct: 142 ENLEGLDRLTNLELGGNKIRVIENLD 167



 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 22/47 (46%), Positives = 31/47 (65%), Gaps = 2/47 (4%)
 Frame = +3

Query: 501 IDRYLQQNNKLSTIYLERNPIA-SDPA-YRRKLKILLPTLHQIDATL 635
           I+  L    KL T+Y E NP+  ++PA YR K+++ LP L QIDAT+
Sbjct: 277 IEDELSGLKKLETVYFEGNPLQKTNPAVYRNKVRLCLPQLRQIDATI 323



 Score = 35.1 bits (77), Expect = 0.010
 Identities = 15/29 (51%), Positives = 23/29 (79%)
 Frame = +2

Query: 167 IKNLEGLRTLKQLYLGKNKISKIQNLEDL 253
           I+NL+ L  L++L++GKNKI+K +N E L
Sbjct: 163 IENLDTLVNLEKLWVGKNKITKFENFEKL 191



 Score = 34.7 bits (76), Expect = 0.014
 Identities = 19/38 (50%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
 Frame = +2

Query: 161 QEIKNLEGLRTLKQLYLGKNKISKIQNLEDLTTW-KYW 271
           + I+NLEGL  L  L LG NKI  I+NL+ L    K W
Sbjct: 139 RRIENLEGLDRLTNLELGGNKIRVIENLDTLVNLEKLW 176



 Score = 29.5 bits (63), Expect = 0.51
 Identities = 12/29 (41%), Positives = 21/29 (72%)
 Frame = +2

Query: 167 IKNLEGLRTLKQLYLGKNKISKIQNLEDL 253
           I+N+  L+ L+ L+  +N+I +I+NLE L
Sbjct: 119 IRNINHLKGLENLFFVQNRIRRIENLEGL 147



 Score = 29.1 bits (62), Expect = 0.68
 Identities = 13/45 (28%), Positives = 26/45 (57%)
 Frame = +1

Query: 253 DNLEILVLQANRLTKIENLQQLNKLDQLYISENGITVIENLENQI 387
           +NLEIL +  N +  +  L  L  L +L+ S N ++  + +E+++
Sbjct: 237 ENLEILDVSNNMIKHLSYLAGLKNLVELWASNNELSSFQEIEDEL 281



 Score = 26.6 bits (56), Expect = 3.6
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = +2

Query: 161 QEIKNLEGLRTLKQLYLGKNKISKIQNLED 250
           + +  L GL+ L +L+   N++S  Q +ED
Sbjct: 250 KHLSYLAGLKNLVELWASNNELSSFQEIED 279


>SPAC926.06c |||leucine-rich repeat protein,
           unknown|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 621

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 32/98 (32%), Positives = 49/98 (50%), Gaps = 8/98 (8%)
 Frame = +1

Query: 268 LVLQANRLTKIENLQQ--LNKLDQLYISENGITVIENLENQIKLQTLDLAVNRITVIENV 441
           L L +N++T         L+ L  L +S N +T +  LEN   L+ LD+  N IT +   
Sbjct: 384 LNLASNKITGCRTFYHISLSHLQILVLSRNHLTSLSGLENVPSLEKLDIRDNSITDVVEF 443

Query: 442 RHM---SDLEELWLNDNQITE-WSS--IDICNKTTNYP 537
           R +   ++ EE +L+ N  T+ +SS  I I N    YP
Sbjct: 444 RRLVGNTNFEEAYLSLNPFTKTYSSYRITIFNYFREYP 481


>SPBC1861.08c |||U2 snRNP-associated protein Lea1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 239

 Score = 32.3 bits (70), Expect = 0.073
 Identities = 27/84 (32%), Positives = 42/84 (50%), Gaps = 6/84 (7%)
 Frame = +1

Query: 298 IENLQQLNKL-DQLYISENGITVIENLENQIKLQTLDLAVNRITVI--ENVRHMSDLEEL 468
           IENL  L  + D +  ++N I  + N     +LQTL    NRIT I  +  + + +L+ L
Sbjct: 33  IENLGVLRDVHDAIDFTDNDIRYLGNFPRMKRLQTLLCGNNRITAIAPDIGKVLPNLKTL 92

Query: 469 WLNDNQITEWSSID---ICNKTTN 531
            L  N + E + +D    C + TN
Sbjct: 93  SLAQNHLQEIADLDPLASCPQLTN 116


>SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog
           Chs2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 926

 Score = 30.3 bits (65), Expect = 0.29
 Identities = 19/38 (50%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = +3

Query: 342 LREWYNSYRK-FGESNKTSDLGPGREQDHCYRKCSAYE 452
           L   YNS  K F  SN TS       QDHCYRK  +YE
Sbjct: 807 LNSVYNSSLKNFSSSNDTSVYHD--VQDHCYRKPQSYE 842


>SPCC1739.11c |cdc11||SIN component scaffold protein
           Cdc11|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1045

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 12/30 (40%), Positives = 23/30 (76%)
 Frame = +2

Query: 164 EIKNLEGLRTLKQLYLGKNKISKIQNLEDL 253
           +I++L+ L+ + +LYL  N+I K++NL D+
Sbjct: 905 DIESLKPLQMIHRLYLVGNRIKKMRNLCDI 934


>SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 609

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -3

Query: 221 YFYPSIIV*GSSNLPSFLFPVKIQNTYSQY 132
           Y  PSI V G  NLP  + PV++ N +S +
Sbjct: 264 YDNPSIFVIGILNLPLKVSPVELYNEFSNH 293


>SPBC800.06 |brx1||ribosome biogenesis protein
           Brx1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 295

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 19/69 (27%), Positives = 35/69 (50%)
 Frame = +1

Query: 283 NRLTKIENLQQLNKLDQLYISENGITVIENLENQIKLQTLDLAVNRITVIENVRHMSDLE 462
           ++L   + L QLN+L +LY + N I   E+   +     +  A N  TV  +V ++  ++
Sbjct: 75  SKLDSKDRLYQLNELAELY-NCNNIFFFESRRREDLYLHIARAPNGPTVKFHVENLHTMD 133

Query: 463 ELWLNDNQI 489
           EL +  N +
Sbjct: 134 ELNMTGNAL 142


>SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 377

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 9/23 (39%), Positives = 15/23 (65%)
 Frame = -1

Query: 172 FYFLLKYKIPTLNIYYTKKLCEV 104
           +Y +L+YK+P   + Y + LC V
Sbjct: 198 YYRVLQYKVPGNELIYRENLCSV 220


>SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 844

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 14/47 (29%), Positives = 28/47 (59%)
 Frame = +2

Query: 137 ESRYFVF*QEIKNLEGLRTLKQLYLGKNKISKIQNLEDLTTWKYWFF 277
           + RY+++ Q+I N+ GL+  K +YL K  ++++ N +      Y+ F
Sbjct: 618 KDRYYMYKQDIDNVVGLKDFK-VYL-KTTLNRLYNSKLTRKVLYYLF 662


>SPAC11E3.14 |||conserved protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 588

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 4/44 (9%)
 Frame = +2

Query: 161 QEIKNLEGLRTLKQLYLGKNKISK----IQNLEDLTTWKYWFFR 280
           ++I  ++G  T   LY+G  K  +    I  LEDL +W + F+R
Sbjct: 324 EQIIAMKGFDTA-MLYVGMRKFKQAADAIIELEDLNSWSHAFYR 366


>SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 658

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 10/28 (35%), Positives = 14/28 (50%)
 Frame = +2

Query: 188 RTLKQLYLGKNKISKIQNLEDLTTWKYW 271
           R  K+  +    ISK+Q   D+  W YW
Sbjct: 569 RDFKRTLITMKAISKLQKNFDVRIWNYW 596


>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
           component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 536

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 2/75 (2%)
 Frame = +1

Query: 418 RITVIENVRHMSDLEELWLNDNQITEWSSIDICNKTTNYPQYI--WRGTR*LQIQLTEGN 591
           RI  +   + M  LEELW ++N ++   ++ + NK     Q I  W+    +QI   E N
Sbjct: 229 RIEFLRLSKRMKKLEELWTSEN-VSMLDAL-LLNKAKRLEQSIVHWQDDSVVQI-CPECN 285

Query: 592 *RYCFQHYTRSTRHC 636
             +     TR  RHC
Sbjct: 286 NSFTL---TRRRRHC 297


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,162,093
Number of Sequences: 5004
Number of extensions: 70688
Number of successful extensions: 229
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)