BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0149.Seq (623 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 32 0.059 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.10 SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar... 31 0.14 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 30 0.24 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.31 SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 27 2.9 SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces pomb... 26 3.8 SPAC3H8.08c |||transcription factor|Schizosaccharomyces pombe|ch... 26 5.1 SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 25 6.7 >SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 494 Score = 32.3 bits (70), Expect = 0.059 Identities = 16/29 (55%), Positives = 20/29 (68%) Frame = +1 Query: 388 PVPPVVKAPRSPMIPPSAPRSLGSASCDS 474 P PP V ++P IPP APR+L +AS DS Sbjct: 379 PKPPSVPTIQAP-IPPEAPRNLTNASVDS 406 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 31.5 bits (68), Expect = 0.10 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 2/40 (5%) Frame = +1 Query: 388 PVPPVVKAPRSPMIPPSAPRSL--GSASCDSVPGSPGVSP 501 P PP + P +PPSAP SL G+ + +P S ++P Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457 Score = 27.9 bits (59), Expect = 1.3 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +1 Query: 364 PPGMELDRPVPPVVKAPRSPMIPPSAPRSLGSASCDSVPGSPGVSPYA 507 PP + P + AP +P +PPSAP + +P +P + P A Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAP--IAPPLPAGMPAAPPLPPAA 473 Score = 26.6 bits (56), Expect = 2.9 Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 9/46 (19%) Frame = +1 Query: 391 VPPVVKA--------PRSPMIPPSAPRSLGSASCDSVP-GSPGVSP 501 +PP+ A P P +PPSAP SL ++ S+P G+P P Sbjct: 403 LPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPP 448 Score = 25.0 bits (52), Expect = 8.9 Identities = 12/28 (42%), Positives = 15/28 (53%), Gaps = 1/28 (3%) Frame = +1 Query: 364 PPGMELDRPVPPVVKA-PRSPMIPPSAP 444 P GM P+PP A P +P P+AP Sbjct: 460 PAGMPAAPPLPPAAPAPPPAPAPAPAAP 487 >SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 653 Score = 31.1 bits (67), Expect = 0.14 Identities = 17/43 (39%), Positives = 22/43 (51%) Frame = +1 Query: 367 PGMELDRPVPPVVKAPRSPMIPPSAPRSLGSASCDSVPGSPGV 495 PGM PVP A PM+ P + S G A ++VP PG+ Sbjct: 530 PGMNARGPVP----AQGRPMMMPGSVPSAGPAEAEAVPAVPGM 568 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 30.3 bits (65), Expect = 0.24 Identities = 19/48 (39%), Positives = 23/48 (47%), Gaps = 3/48 (6%) Frame = +1 Query: 364 PPGMELDRPVPPVVKA-PRSP--MIPPSAPRSLGSASCDSVPGSPGVS 498 PPGM P P V A P P PP AP S ++ ++ PGVS Sbjct: 532 PPGMPAPFPGYPAVPAMPGIPGATAPPGAPGSYNTSESSNLNAPPGVS 579 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 29.9 bits (64), Expect = 0.31 Identities = 22/50 (44%), Positives = 25/50 (50%), Gaps = 7/50 (14%) Frame = +1 Query: 364 PPGMELDRP------VPPVVKAPRSPMIPPSA-PRSLGSASCDSVPGSPG 492 PP M P VPP AP P PPSA P L ++S SVP +PG Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP-NPG 1747 Score = 27.1 bits (57), Expect = 2.2 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 4/48 (8%) Frame = +1 Query: 358 IQP--PGMELDRPVPPVVKAPRSPM--IPPSAPRSLGSASCDSVPGSP 489 +QP PGM + P P AP +P+ +PP+ P + SV P Sbjct: 1458 VQPKAPGMVTNAPAPSSAPAPPAPVSQLPPAVPNVPVPSMIPSVAQQP 1505 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 26.6 bits (56), Expect = 2.9 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -3 Query: 84 EPIDTIAGSSSAVYAAKLARVVTHAQ 7 EP+ IA +YAAK+A +HA+ Sbjct: 145 EPVSIIADHIEIIYAAKIAAHASHAK 170 >SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 478 Score = 26.2 bits (55), Expect = 3.8 Identities = 12/34 (35%), Positives = 15/34 (44%) Frame = +1 Query: 430 PPSAPRSLGSASCDSVPGSPGVSPYAHRRQCLRR 531 PP PR+L S+ D+ VS H R R Sbjct: 292 PPEVPRNLNSSLVDAAESLANVSQQQHHRHPFAR 325 >SPAC3H8.08c |||transcription factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 563 Score = 25.8 bits (54), Expect = 5.1 Identities = 13/46 (28%), Positives = 21/46 (45%) Frame = +2 Query: 122 SEPGASSSGVPRAPPNCARCRNHRLKIELKGHKRYCKYQHCTCESA 259 S P A R+P +C CR ++K + + CK ++ C A Sbjct: 3 SSPPALKKFRKRSPKSCLICRRRKVKCDRQQPCSRCKERNEVCTYA 48 >SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.4 bits (53), Expect = 6.7 Identities = 9/23 (39%), Positives = 13/23 (56%) Frame = +1 Query: 364 PPGMELDRPVPPVVKAPRSPMIP 432 PPGM + P P P +P++P Sbjct: 293 PPGMVMASPSPAAATIPGAPVMP 315 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,311,850 Number of Sequences: 5004 Number of extensions: 42953 Number of successful extensions: 176 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 275671126 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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