Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0143.Seq
         (754 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom...   108   1e-24
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom...   106   3e-24
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ...    44   3e-05
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe...    33   0.044
SPAC29B12.14c |||purine transporter |Schizosaccharomyces pombe|c...    27   2.9  
SPAC1952.07 |rad1||checkpoint clamp complex protein Rad1|Schizos...    27   2.9  
SPBC146.09c |lsd1|swm1, saf110|histone demethylase SWIRM1|Schizo...    27   3.8  
SPBC1861.01c |cnp3|SPBC56F2.13|CENP-C|Schizosaccharomyces pombe|...    26   6.6  
SPAC977.12 |||L-asparaginase |Schizosaccharomyces pombe|chr 1|||...    25   8.8  
SPAC17G6.04c |cpp1||protein farnesyltransferase beta subunit Cpp...    25   8.8  
SPBPB8B6.05c |||L-asparaginase |Schizosaccharomyces pombe|chr 2|...    25   8.8  
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar...    25   8.8  

>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score =  108 bits (259), Expect = 1e-24
 Identities = 46/81 (56%), Positives = 66/81 (81%)
 Frame = +1

Query: 511 IRQNTEGEYAMLEHESVNGVVESMKVVTADNSERVARFAFEFAKKNGRKKVTTVHKANIM 690
           IR+NTEGEY+ +EHE + GVV+S+K++T   SERV R+AF++A++ G+  +T VHKA IM
Sbjct: 158 IRENTEGEYSGIEHEVIPGVVQSIKLITRAASERVIRYAFQYARQTGKNNITVVHKATIM 217

Query: 691 KLSDGLFLETSRRLAKEYPDI 753
           +++DGLFLE ++ LA EYPDI
Sbjct: 218 RMADGLFLECAKELAPEYPDI 238



 Score = 31.1 bits (67), Expect = 0.18
 Identities = 14/26 (53%), Positives = 16/26 (61%)
 Frame = +3

Query: 174 GRHAVTMLPGGGIGPECMGYVRDIFK 251
           G + VTM+ G GIGPE    V  IFK
Sbjct: 44  GNYTVTMIAGDGIGPEIAQSVERIFK 69


>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 356

 Score =  106 bits (255), Expect = 3e-24
 Identities = 50/81 (61%), Positives = 64/81 (79%)
 Frame = +1

Query: 511 IRQNTEGEYAMLEHESVNGVVESMKVVTADNSERVARFAFEFAKKNGRKKVTTVHKANIM 690
           IR+NTEGEY+ LEH+SV GVVES+K++T   S+R+A+FAF+FA +NGRK VT +HKANIM
Sbjct: 136 IRENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNGRKSVTCIHKANIM 195

Query: 691 KLSDGLFLETSRRLAKEYPDI 753
           KL+DGLF  T   +A  Y  I
Sbjct: 196 KLADGLFRRTFYDVANGYDAI 216



 Score = 69.7 bits (163), Expect = 4e-13
 Identities = 39/93 (41%), Positives = 57/93 (61%), Gaps = 5/93 (5%)
 Frame = +2

Query: 263 PIDFEVVDIDPTMDND----DDVQYAITTIKRNGVGLKGNIETKSEAAYVTSRNVALRNE 430
           PI+FE +D+     N+    D +  AI ++KRN VGLKG + T  E    TS NVALR E
Sbjct: 49  PIEFEEIDVTGMEKNNKSSGDALHEAIQSLKRNKVGLKGILFTPFEKGGHTSFNVALRKE 108

Query: 431 LDMYAYILNCKSYPGVATRHKDID-VVLSDRTQ 526
           LD+YA ++  K+ PG  TRH ++D  ++ + T+
Sbjct: 109 LDIYASLVLIKNIPGFKTRHDNVDFAIIRENTE 141



 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 17/40 (42%), Positives = 25/40 (62%)
 Frame = +3

Query: 132 VIRKQKLIPKAQYGGRHAVTMLPGGGIGPECMGYVRDIFK 251
           ++RK       +YGG++ VT++PG GIG E    V +IFK
Sbjct: 5   LVRKSSAFQPLKYGGKYTVTLIPGDGIGRETSNAVTEIFK 44


>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 362

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 28/88 (31%), Positives = 49/88 (55%), Gaps = 16/88 (18%)
 Frame = +1

Query: 511 IRQNTEGEYA----MLEHESVNGVVESMKVVTADNSERVARFAFEFAKKNGR-------- 654
           +R+NTE  Y     M+++     V E+++ ++ + S ++ + AFE AK   +        
Sbjct: 125 VRENTECLYVKEERMVQNTPGKRVAEAIRRISEEASTKIGKMAFEIAKSRQKIRESGTYS 184

Query: 655 --KK--VTTVHKANIMKLSDGLFLETSR 726
             KK  VT +HK+N+M ++DGLF E+ R
Sbjct: 185 IHKKPLVTIIHKSNVMSVTDGLFRESCR 212


>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 734

 Score = 33.1 bits (72), Expect = 0.044
 Identities = 19/74 (25%), Positives = 36/74 (48%)
 Frame = +2

Query: 230 IRSRHIQILGAPIDFEVVDIDPTMDNDDDVQYAITTIKRNGVGLKGNIETKSEAAYVTSR 409
           +R R  ++  + +   V+     M +DD VQY I  +K  G+ L  +++T  E   +  +
Sbjct: 664 VRDRQFELQASIV--RVMKQKEKMKHDDLVQYVINNVKDRGIPLVSDVKTAIEK--LLEK 719

Query: 410 NVALRNELDMYAYI 451
               R + D+Y Y+
Sbjct: 720 EYLEREDNDIYTYV 733


>SPAC29B12.14c |||purine transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 581

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
 Frame = +2

Query: 347 NGVGLKGNIETKSEAAYVTSRNVA--LRNELDMYAYILNCKSYPGVATRHKDID 502
           N VGL G +  K   A +T  N+A  L       ++++ CK +P  A   K +D
Sbjct: 470 NVVGLAGAVGDKVPKAALTMNNIAYLLGIVTSFLSHLIICKIFPVTACGEKFLD 523


>SPAC1952.07 |rad1||checkpoint clamp complex protein
           Rad1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 323

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 11/46 (23%), Positives = 23/46 (50%), Gaps = 2/46 (4%)
 Frame = -3

Query: 575 STTPFTDSCSNIAYSPSVFCLIKQHQ--CPYVWSQRQGKIYSSECK 444
           ST+ +     NI +   V C ++ +   CP++W   +   Y++ C+
Sbjct: 93  STSAWDQPTVNIMHKRGVICKVQYNGPGCPFIWEVEEMAGYATACE 138


>SPBC146.09c |lsd1|swm1, saf110|histone demethylase
           SWIRM1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1000

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 13/49 (26%), Positives = 25/49 (51%)
 Frame = +1

Query: 574 ESMKVVTADNSERVARFAFEFAKKNGRKKVTTVHKANIMKLSDGLFLET 720
           E++ +V    +  +A  AFEF  +NG      ++  + +KL + L  +T
Sbjct: 211 EALGIVREKKAFPLASLAFEFLSRNGHINYGCIYIISSLKLDESLSQKT 259


>SPBC1861.01c |cnp3|SPBC56F2.13|CENP-C|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 643

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 27/94 (28%), Positives = 46/94 (48%), Gaps = 6/94 (6%)
 Frame = +2

Query: 122 QDSRYQETEADS*GAIRRSSRGDHAP---WRRYRS*VHGIRSRHIQILGAPIDFEVVDID 292
           Q+ +  E E D+   +RRS R   AP   W+  R  V+ +     +I   P   +++ +D
Sbjct: 401 QEIKQVERE-DTLVGVRRSKRTRIAPLAFWKNERV-VYELHRDENRIPALPEVKQIIRVD 458

Query: 293 ---PTMDNDDDVQYAITTIKRNGVGLKGNIETKS 385
              P++      ++A    KR+GV +K N+E KS
Sbjct: 459 DPSPSIRQGRKKRHA----KRSGVEIKSNLEAKS 488


>SPAC977.12 |||L-asparaginase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 356

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 13/34 (38%), Positives = 17/34 (50%)
 Frame = -3

Query: 515 LIKQHQCPYVWSQRQGKIYSSECKHTCPVRF*EP 414
           LI++H  P V+S R  + YSS      P  F  P
Sbjct: 296 LIREHNIPVVYSHRTAEGYSSNSCLGIPSYFLNP 329


>SPAC17G6.04c |cpp1||protein farnesyltransferase beta subunit
           Cpp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 382

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 4/39 (10%)
 Frame = -2

Query: 381 LVSMLPFSPTPFLLMVV----IAYWTSSSLSIVGSMSTT 277
           L  ML   P+PF ++      + YW  SSL+I+G + ++
Sbjct: 38  LTKMLDPLPSPFTVLDASRAWMVYWELSSLAILGKLDSS 76


>SPBPB8B6.05c |||L-asparaginase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 356

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 13/34 (38%), Positives = 17/34 (50%)
 Frame = -3

Query: 515 LIKQHQCPYVWSQRQGKIYSSECKHTCPVRF*EP 414
           LI++H  P V+S R  + YSS      P  F  P
Sbjct: 296 LIREHNIPVVYSHRTAEGYSSNSCLGIPSYFLNP 329


>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1131

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = -3

Query: 635 NSKANLATLSELSAVTTFIDSTTPFTDSCSNIAYSPS 525
           NS  + ++ S+  + TT+ D TT  T   S+I  SP+
Sbjct: 689 NSTGSSSSDSQSFSGTTYSDPTTTITSEVSSILSSPT 725


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,249,266
Number of Sequences: 5004
Number of extensions: 69203
Number of successful extensions: 216
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)