BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0135X.Seq (548 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g31812.1 68414.m03905 acyl-CoA binding protein / ACBP identic... 63 1e-10 At3g05420.2 68416.m00594 acyl-CoA binding family protein similar... 29 2.0 At3g05420.1 68416.m00593 acyl-CoA binding family protein similar... 29 2.0 At3g13620.1 68416.m01714 amino acid permease family protein weak... 29 2.7 At4g04070.1 68417.m00576 hypothetical protein low similarity to ... 27 6.2 At2g42790.1 68415.m05298 citrate synthase, glyoxysomal, putative... 27 8.3 At1g61370.1 68414.m06916 S-locus lectin protein kinase family pr... 27 8.3 >At1g31812.1 68414.m03905 acyl-CoA binding protein / ACBP identical to acyl-CoA-binding protein (ACBP) [Arabidopsis thaliana] SWISS-PROT:P57752 Length = 92 Score = 63.3 bits (147), Expect = 1e-10 Identities = 30/72 (41%), Positives = 45/72 (62%) Frame = +1 Query: 31 MSLQEKFDQAAANVKNLKALPTDAQLLNLYAHFKQATVGDADPANRPGLLDLKGKAKFDA 210 M L+E+F++ A V L LP++ LL LY +KQA G D +RPG+ +K +AK+DA Sbjct: 1 MGLKEEFEEHAEKVNTLTELPSNEDLLILYGLYKQAKFGPVD-TSRPGMFSMKERAKWDA 59 Query: 211 WHKLAGTSKDDA 246 W + G S ++A Sbjct: 60 WKAVEGKSSEEA 71 >At3g05420.2 68416.m00594 acyl-CoA binding family protein similar to PIR|S68824|S68824 rngB protein, cytosolic (Dictyostelium discoideum); contains Pfam profiles PF00887: Acyl CoA binding protein, PF01344: Kelch motif Length = 669 Score = 29.1 bits (62), Expect = 2.0 Identities = 21/67 (31%), Positives = 33/67 (49%) Frame = +1 Query: 52 DQAAANVKNLKALPTDAQLLNLYAHFKQATVGDADPANRPGLLDLKGKAKFDAWHKLAGT 231 D +A NV + P D LL LYA ++QATVG + +P ++K+ +W L Sbjct: 29 DSSAKNV--ISKFPDDTALL-LYALYQQATVGPCN-TPKPSAWRPVEQSKWKSWQGLGTM 84 Query: 232 SKDDAQK 252 +A + Sbjct: 85 PSIEAMR 91 >At3g05420.1 68416.m00593 acyl-CoA binding family protein similar to PIR|S68824|S68824 rngB protein, cytosolic (Dictyostelium discoideum); contains Pfam profiles PF00887: Acyl CoA binding protein, PF01344: Kelch motif Length = 668 Score = 29.1 bits (62), Expect = 2.0 Identities = 21/67 (31%), Positives = 33/67 (49%) Frame = +1 Query: 52 DQAAANVKNLKALPTDAQLLNLYAHFKQATVGDADPANRPGLLDLKGKAKFDAWHKLAGT 231 D +A NV + P D LL LYA ++QATVG + +P ++K+ +W L Sbjct: 29 DSSAKNV--ISKFPDDTALL-LYALYQQATVGPCN-TPKPSAWRPVEQSKWKSWQGLGTM 84 Query: 232 SKDDAQK 252 +A + Sbjct: 85 PSIEAMR 91 >At3g13620.1 68416.m01714 amino acid permease family protein weak similarity to SP|Q9WTR6 Cystine/glutamate transporter (Amino acid transport system xc-) {Mus musculus}; contains Pfam profile PF00324: Amino acid permease Length = 478 Score = 28.7 bits (61), Expect = 2.7 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -1 Query: 122 AYKLRSWASVGRAFRFFTLAAAWSNFSWRDILI 24 AY+L A +G +FF + + W N W ILI Sbjct: 314 AYQLEGMAELGFLPKFFGVRSKWFNTPWVGILI 346 >At4g04070.1 68417.m00576 hypothetical protein low similarity to protective antigen [Streptococcus pyogenes] GI:8996050, fibrinogen-binding protein [Streptococcus equi] GI:3093478 Length = 728 Score = 27.5 bits (58), Expect = 6.2 Identities = 19/67 (28%), Positives = 31/67 (46%) Frame = +2 Query: 95 LMPNSLTCMPISNRPQLEMPIQPIDLVF*T*RVKPNLTLGTNWPALRRMMPRKLHRDRRR 274 ++P + PI P++E P+ DL + P+ + + P +RRM LH R Sbjct: 224 IVPPTAQLDPIEEVPRIE-PVPQADLPI----IPPDSSSTSELPLIRRMRCPSLHVQRAN 278 Query: 275 AHSFHRA 295 A + RA Sbjct: 279 AEAHARA 285 >At2g42790.1 68415.m05298 citrate synthase, glyoxysomal, putative strong similarity to SP|P49299 Citrate synthase, glyoxysomal precursor {Cucurbita maxima}; contains Pfam profile PF00285: Citrate synthase Length = 509 Score = 27.1 bits (57), Expect = 8.3 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +3 Query: 336 DNFHYRVYYINTRAFSHHQRTSDFFDAIFILH 431 DNF Y + + R++ + R + D +FILH Sbjct: 250 DNFLYMLDSLGNRSYKPNPRLARVLDILFILH 281 >At1g61370.1 68414.m06916 S-locus lectin protein kinase family protein contains Pfam domains, PF00954: S-locus glycoprotein family, PF00069: Protein kinase domain, and PF01453: Lectin (probable mannose binding) Length = 814 Score = 27.1 bits (57), Expect = 8.3 Identities = 15/41 (36%), Positives = 16/41 (39%) Frame = -1 Query: 248 WASSFEVPASLCQASNLALPFKSRRPGLLAGSASPTVACLK 126 W + E P S C N PF GL S P CLK Sbjct: 275 WVTDLEAPVSSCDVYNTCGPF-----GLCIRSNPPKCECLK 310 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,871,137 Number of Sequences: 28952 Number of extensions: 241937 Number of successful extensions: 586 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 573 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 585 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1033331880 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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