Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0125.Seq
(652 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 136 1e-32
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 134 8e-32
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 132 2e-31
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 117 1e-26
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 71 6e-13
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 65 4e-11
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 55 4e-08
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 6e-04
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.003
SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08) 33 0.20
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.81
SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.5
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.5
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 4.3
SB_53017| Best HMM Match : Kazal_1 (HMM E-Value=0) 28 5.7
SB_42630| Best HMM Match : Ets (HMM E-Value=0) 28 7.6
SB_34715| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.6
SB_7343| Best HMM Match : EGF (HMM E-Value=0) 28 7.6
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 136 bits (329), Expect = 1e-32
Identities = 61/86 (70%), Positives = 71/86 (82%)
Frame = +1
Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEVVNYGGKPKIKVAYKGEDKTFFPEEVSSM 429
P NT+FDAKRLIGRKF+D V DM HWPF V+ G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 430 VLTKMKETAEAYLGKTVQNAVITVPA 507
VLTKMKETAEAYLG V +AV+TVPA
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPA 148
Score = 132 bits (318), Expect = 3e-31
Identities = 61/63 (96%), Positives = 62/63 (98%)
Frame = +2
Query: 65 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 244
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 245 MNP 253
MNP
Sbjct: 61 MNP 63
Score = 85.0 bits (201), Expect = 5e-17
Identities = 42/47 (89%), Positives = 44/47 (93%)
Frame = +3
Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 650
F+DSQRQATKDAGTI+GLNVLRIINEPTAAAIAYGLDKK ERNVL
Sbjct: 150 FHDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGVERNVL 196
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 134 bits (323), Expect = 8e-32
Identities = 62/86 (72%), Positives = 74/86 (86%)
Frame = +1
Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEVVNYGGKPKIKVAYKGEDKTFFPEEVSSM 429
P NTIFDAKRLIGR+++DATVQ+DMK WPF++++ KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 430 VLTKMKETAEAYLGKTVQNAVITVPA 507
VL KMKETAEAYLG+ V +AVITVPA
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPA 148
Score = 127 bits (306), Expect = 9e-30
Identities = 57/63 (90%), Positives = 61/63 (96%)
Frame = +2
Query: 65 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 244
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 245 MNP 253
+NP
Sbjct: 61 LNP 63
Score = 87.0 bits (206), Expect = 1e-17
Identities = 40/47 (85%), Positives = 45/47 (95%)
Frame = +3
Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 650
FNDSQRQATKDAGTI+GLNVLR+INEPTAAA+AYGLDK +GE+NVL
Sbjct: 150 FNDSQRQATKDAGTIAGLNVLRVINEPTAAALAYGLDKSLSGEKNVL 196
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 132 bits (319), Expect = 2e-31
Identities = 60/64 (93%), Positives = 62/64 (96%)
Frame = +2
Query: 65 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 244
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 245 MNPT 256
MNPT
Sbjct: 61 MNPT 64
Score = 114 bits (274), Expect = 7e-26
Identities = 65/111 (58%), Positives = 72/111 (64%), Gaps = 25/111 (22%)
Frame = +1
Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEVVNYGGKPKIKVAYKGEDKTFFPEEV--- 420
P NTIFDAKRLIGR+F+D V+ DMKHW FEVVN G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 421 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPA 507
SSMVL KMKETAEAYLG V +AV+TVPA
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPA 173
Score = 86.6 bits (205), Expect = 2e-17
Identities = 43/47 (91%), Positives = 43/47 (91%)
Frame = +3
Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 650
FNDSQRQATKDAG ISGLNVLRIINEPTAAAIAYGLDKK ERNVL
Sbjct: 243 FNDSQRQATKDAGVISGLNVLRIINEPTAAAIAYGLDKKVGQERNVL 289
Score = 49.2 bits (112), Expect = 3e-06
Identities = 23/31 (74%), Positives = 27/31 (87%)
Frame = +1
Query: 415 EVSSMVLTKMKETAEAYLGKTVQNAVITVPA 507
++SSMVL KMKETAEAYLG V +AV+TVPA
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPA 241
Score = 31.1 bits (67), Expect = 0.81
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +3
Query: 510 FNDSQRQATKDAG 548
FNDSQRQATKDAG
Sbjct: 175 FNDSQRQATKDAG 187
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 117 bits (281), Expect = 1e-26
Identities = 55/86 (63%), Positives = 69/86 (80%)
Frame = +1
Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEVVNYGGKPKIKVAYKGEDKTFFPEEVSSM 429
P NT+FDAKRLIGR ++D +VQ D+K +PF+VV+ KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 430 VLTKMKETAEAYLGKTVQNAVITVPA 507
VLTKMKETAEAYLGK V +AV+TVPA
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPA 846
Score = 99 bits (238), Expect = 2e-21
Identities = 46/58 (79%), Positives = 52/58 (89%), Gaps = 1/58 (1%)
Frame = +2
Query: 83 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNP 253
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNP 761
Score = 79.0 bits (186), Expect = 3e-15
Identities = 37/47 (78%), Positives = 43/47 (91%)
Frame = +3
Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 650
FND+QRQATKDAG I+GL V+RIINEPTAAAIAYG+DKK GE+N+L
Sbjct: 848 FNDAQRQATKDAGAIAGLTVMRIINEPTAAAIAYGMDKK-EGEKNIL 893
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 71.3 bits (167), Expect = 6e-13
Identities = 37/85 (43%), Positives = 52/85 (61%), Gaps = 1/85 (1%)
Frame = +1
Query: 256 NTIFDAKRLIGRKFEDATVQADMKHWPFEVVNYGGKP-KIKVAYKGEDKTFFPEEVSSMV 432
NTI KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M+
Sbjct: 121 NTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAML 180
Query: 433 LTKMKETAEAYLGKTVQNAVITVPA 507
T++K TAE L V + VI+VP+
Sbjct: 181 FTRLKTTAEIALKTKVTDCVISVPS 205
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +2
Query: 83 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 238
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 42.3 bits (95), Expect = 3e-04
Identities = 18/48 (37%), Positives = 29/48 (60%)
Frame = +3
Query: 483 ECSYHGSRVFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 626
+C + D QR+ DA +GLN LR++N+ TA ++AYG+ K+
Sbjct: 198 DCVISVPSYYTDRQRRCMLDASATAGLNCLRLMNDTTAVSLAYGIYKQ 245
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 65.3 bits (152), Expect = 4e-11
Identities = 29/63 (46%), Positives = 43/63 (68%), Gaps = 2/63 (3%)
Frame = +2
Query: 71 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 244
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 245 MNP 253
+NP
Sbjct: 90 INP 92
Score = 55.2 bits (127), Expect = 4e-08
Identities = 33/87 (37%), Positives = 44/87 (50%), Gaps = 1/87 (1%)
Frame = +1
Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEVV-NYGGKPKIKVAYKGEDKTFFPEEVSS 426
P NTI+DAKR IG+ F + K + F V + G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 427 MVLTKMKETAEAYLGKTVQNAVITVPA 507
V+ K++ AE L TV AV++VPA
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPA 178
Score = 54.8 bits (126), Expect = 6e-08
Identities = 27/47 (57%), Positives = 35/47 (74%)
Frame = +3
Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVL 650
F+ QR AT A +++G+ VLR+INEPTAAA+AYGL KK G NV+
Sbjct: 180 FDIKQRNATVKAASLAGITVLRVINEPTAAALAYGLHKK-EGVANVM 225
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 55.2 bits (127), Expect = 4e-08
Identities = 39/71 (54%), Positives = 42/71 (59%), Gaps = 26/71 (36%)
Frame = +3
Query: 510 FNDSQRQATKDAGTISGLNVL------------------------RIINEPTAAAIAYGL 617
FND+QRQATKDAGTI+GL+VL RIINEPTAAAIAYGL
Sbjct: 49 FNDAQRQATKDAGTIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGL 108
Query: 618 DKK--GTGERN 644
DK G ERN
Sbjct: 109 DKARGGKAERN 119
Score = 52.0 bits (119), Expect = 4e-07
Identities = 24/46 (52%), Positives = 35/46 (76%)
Frame = +1
Query: 370 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPA 507
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPA 47
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 6e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +2
Query: 134 GKVEIIANDQGNRTTPSYVAFTDTERLI 217
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 39.1 bits (87), Expect = 0.003
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = +3
Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 617
FN ++R+A A + GLNVL+I+N TA A+ YGL
Sbjct: 116 FNQAERRALLRAAELVGLNVLQIMNSNTAVALNYGL 151
Score = 34.3 bits (75), Expect = 0.087
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +1
Query: 364 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 504
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVP 113
>SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08)
Length = 1011
Score = 33.1 bits (72), Expect = 0.20
Identities = 25/90 (27%), Positives = 41/90 (45%), Gaps = 4/90 (4%)
Frame = +1
Query: 289 RKFEDATVQADM----KHWPFEVVNYGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETA 456
+K+ ++ VQAD K +V Y +PK K + E+ EE +M ++K
Sbjct: 626 KKWFNSRVQADSHYSGKAHLTQVDLYNMQPKFKKTKEEEEFDKLLEEADAMTQQEVKNVK 685
Query: 457 EAYLGKTVQNAVITVPASSMTLKDKPQKMQ 546
LGK V+ + T ++TL + Q Q
Sbjct: 686 NIKLGKNVKTQLYTCEPCNITLNSQKQMSQ 715
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.81
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +2
Query: 143 EIIANDQGNRTTPSYVAFT-DTERL 214
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 665
Score = 29.5 bits (63), Expect = 2.5
Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Frame = +1
Query: 52 LKIQNGKSTRSRN-RSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCR 228
L++ N + ++ + R GY L R C R + +R + H++L R +RC
Sbjct: 225 LRLVNTRDDKTHDKRPGYRKLSRVC-GGRRPNEPYERTARV-HTILSTETETRFCAKRCA 282
Query: 229 QEPGGDEPHNTIFDA 273
G +P NT F+A
Sbjct: 283 GTASGMDPKNTPFNA 297
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.5
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -3
Query: 251 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 153
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 11 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 97
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_53017| Best HMM Match : Kazal_1 (HMM E-Value=0)
Length = 1488
Score = 28.3 bits (60), Expect = 5.7
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +3
Query: 408 PRGSQFHGAYENEGNCRSLSRQNCAECSYHGSRVFN 515
PRG Q Y G+C S+S++NC + S+ + +N
Sbjct: 1142 PRGKQI--PYGTAGDCYSVSQENCRKGSFKVTYQYN 1175
>SB_42630| Best HMM Match : Ets (HMM E-Value=0)
Length = 631
Score = 27.9 bits (59), Expect = 7.6
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +3
Query: 93 IWVPRTLALVSSSTGRWRSSPTTRATGPLRLMLRSQTPSVSSEMPPRT 236
IW P+ L++S R+S + PLR L P VSS +P T
Sbjct: 42 IWPPKKQHLMASYPSTSRASASRSHLFPLRTGLPPVPPLVSSPLPSPT 89
>SB_34715| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 977
Score = 27.9 bits (59), Expect = 7.6
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +3
Query: 93 IWVPRTLALVSSSTGRWRSSPTTRATGPLRLMLRSQTPSVSSEMPPRT 236
IW P+ L++S R+S + PLR L P VSS +P T
Sbjct: 388 IWPPKKQHLMASYPSTSRASASRSHLFPLRTGLPPVPPLVSSPLPSPT 435
>SB_7343| Best HMM Match : EGF (HMM E-Value=0)
Length = 1233
Score = 27.9 bits (59), Expect = 7.6
Identities = 17/72 (23%), Positives = 29/72 (40%)
Frame = +2
Query: 104 TYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTTQYSMPNVS 283
T++ + H I+ N G +TP + T A + + M PT V+
Sbjct: 559 THTTTSITLHPSSAILDNSLGPASTPRTTISSTTHVSSTPIASSSMVMTPTAINPSATVT 618
Query: 284 SDVSSKMLLCKP 319
+ V S ++ KP
Sbjct: 619 TTVPSTSVVVKP 630
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,590,909
Number of Sequences: 59808
Number of extensions: 534424
Number of successful extensions: 1715
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1569
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1709
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1657237625
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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